LOCUS       sequence-313091-       13415 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 2 Golden Gate vector, 
            pL2M-HvHSP17-CREU5-UBQ-loxmCHERRYER-eGFPER.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13415)
  AUTHORS   Harrington SA, Backhaus AE, Fox S, Rogers C, Borrill P, Uauy C, 
            Richardson A
  TITLE     A heat-shock inducible system for flexible gene expression in 
            cereals.
  JOURNAL   Plant Methods. 2020 Oct 14;16:137. doi: 10.1186/s13007-020-00677-3. 
            eCollection 2020.
  PUBMED    33072173
REFERENCE   2  (bases 1 to 13415)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Methods."; date: "2020-10-14"; volume: "16:137. doi"; pages: " 
            10.1186/s13007-020-00677-3. eCollection 2020"
COMMENT     Sequence Label: EC71174
FEATURES             Location/Qualifiers
     source          1..13415
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    91..115
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        166..2162
                     /label=Ubi promoter
                     /note="maize polyubiquitin gene promoter"
     protein_bind    complement(2173..2206)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             2291..2995
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     polyA_signal    3051..3225
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     protein_bind    complement(3235..3268)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             join(3353..3355,3356..3358,3359..4069)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3353..3355,3356..3358,3359..4069)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3659..3678)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4006..4027
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     intron          5499..5687
                     /label=IV2 intron
                     /note="modified second intron of the potato ST-LS1 gene 
                     (Vancanneyt et al., 1990)"
     terminator      6488..6736
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     promoter        6838..7183
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     7126..7146
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     intron          7527..7716
                     /label=cat1 intron
                     /note="castor bean catalase intron, modified"
     terminator      8416..8668
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    8763..8787
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             9051..9680
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             10109..11182
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGASSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      11248..11442
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     promoter        11725..11829
                     /gene="bla"
                     /label=AmpR promoter
     CDS             11830..12624
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCAKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      12795..13383
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     13284..13303
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gtggttggca catacaaatg gacgaacgga taaacctttt cacgcccttt taaatatccg
       61 attattctaa taaacgctct tttctcttag gtttacccgc caatatatcc tgtcaaacac
      121 tgatagttta aactccctcg gtcacatgtg catcctcgta aggagctgca gtgcagcgtg
      181 acccggtcgt gcccctctct agagataatg agcattgcat gtctaagtta taaaaaatta
      241 ccacatattt tttttgtcac acttgtttga agtgcagttt atctatcttt atacatatat
      301 ttaaacttta ctctacgaat aatataatct atagtactac aataatatca gtgttttaga
      361 gaatcatata aatgaacagt tagacatggt ctaaaggaca attgagtatt ttgacaacag
      421 gactctacag ttttatcttt ttagtgtgca tgtgttctcc tttttttttg caaatagctt
      481 cacctatata atacttcatc cattttatta gtacatccat ttagggttta gggttaatgg
      541 tttttataga ctaatttttt tagtacatct attttattct attttagcct ctaaattaag
      601 aaaactaaaa ctctatttta gtttttttat ttaataattt agatataaaa tagaataaaa
      661 taaagtgact aaaaattaaa caaataccct ttaagaaatt aaaaaaacta aggaaacatt
      721 tttcttgttt cgagtagata atgccagcct gttaaacgcc gtcgacgagt ctaacggaca
      781 ccaaccagcg aaccagcagc gtcgcgtcgg gccaagcgaa gcagacggca cggcatctct
      841 gtcgctgcct ctggacccct ctcgagagtt ccgctccacc gttggacttg ctccgctgtc
      901 ggcatccaga aattgcgtgg cggagcggca gacgtgagcc ggcacggcag gcggcctcct
      961 cctcctctca cggcaccggc agctacgggg gattcctttc ccaccgctcc ttcgctttcc
     1021 cttcctcgcc cgccgtaata aatagacacc ccctccacac cctctttccc caacctcgtg
     1081 ttgttcggag cgcacacaca cacaaccaga tctcccccaa atccacccgt cggcacctcc
     1141 gcttcaaggt acgccgctcg tcctcccccc ccccccctct ctaccttctc tagatcggcg
     1201 ttccggtcca tggttagggc ccggtagttc tacttctgtt catgtttgtg ttagatccgt
     1261 gtttgtgtta gatccgtgct gctagcgttc gtacacggat gcgacctgta cgtcagacac
     1321 gttctgattg ctaacttgcc agtgtttctc tttggggaat cctgggatgg ctctagccgt
     1381 tccgcagacg ggatcgattt catgattttt tttgtttcgt tgcatagggt ttggtttgcc
     1441 cttttccttt atttcaatat atgccgtgca cttgtttgtc gggtcatctt ttcatgcttt
     1501 tttttgtctt ggttgtgatg atgtggtctg gttgggcggt cgttctagat cggagtagaa
     1561 ttaattctgt ttcaaactac ctggtggatt tattaatttt ggatctgtat gtgtgtgcca
     1621 tacatattca tagttacgaa ttgaagatga tggatggaaa tatcgatcta ggataggtat
     1681 acatgttgat gcgggtttta ctgatgcata tacagagatg ctttttgttc gcttggttgt
     1741 gatgatgtgg tgtggttggg cggtcgttca ttcgttctag atcggagtag aatactgttt
     1801 caaactacct ggtgtattta ttaattttgg aactgtatgt gtgtgtcata catcttcata
     1861 gttacgagtt taagatggat ggaaatatcg atctaggata ggtatacatg ttgatgtggg
     1921 ttttactgat gcatatacat gatggcatat gcagcatcta ttcatatgct ctaaccttga
     1981 gtacctatct attataataa acaagtatgt tttataatta ttttgatctt gatatacttg
     2041 gatgatggca tatgcagcag ctatatgtgg atttttttag ccctgccttc atacgctatt
     2101 tatttgcttg gtactgtttc ttttgtcgat gctcaccctg ttgtttggtg ttacttctgc
     2161 agtactgacc taataacttc gtatagcata cattatacga agttatatta agggttgaat
     2221 gaaaactaat ctttttctct ttctcatctt ttcacttctc ctatcattat cctcggccga
     2281 attcggaggt gtgagcaagg gcgaggagga taacatggcc atcatcaagg agttcatgcg
     2341 cttcaaggtg cacatggagg gctccgtgaa cggccacgag ttcgagatcg agggcgaggg
     2401 cgagggccgc ccctacgagg gcacccagac cgccaagctg aaggtgacca agggtggccc
     2461 cctgcccttc gcctgggaca tcctgtcccc tcagttcatg tacggctcca aggcctacgt
     2521 gaagcacccc gccgacatcc ccgactactt gaagctgtcc ttccccgagg gcttcaagtg
     2581 ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc gtgacccagg actcctccct
     2641 gcaggacggc gagttcatct acaaggtgaa gctgcgcggc accaacttcc cctccgacgg
     2701 ccccgtaatg cagaagaaaa ccatgggctg ggaggcctcc tccgagcgga tgtaccccga
     2761 ggacggcgcc ctgaagggcg agatcaagca gaggctgaag ctgaaggacg gcggccacta
     2821 cgacgctgag gtcaagacca cctacaaggc caagaagccc gtgcagctgc ccggcgccta
     2881 caacgtcaac atcaagttgg acatcacctc ccacaacgag gactacacca tcgtggaaca
     2941 gtacgaacgc gccgagggcc gccactccac cggcggcatg gacgagctgt acaagggtgg
     3001 ccatgatgag ctttaagctt ctctagctag agtcgatcga caagctcgag tttctccata
     3061 ataatgtgtg agtagttccc agataaggga attagggttc ctatagggtt tcgctcatgt
     3121 gttgagcata taagaaaccc ttagtatgta tttgtatttg taaaatactt ctatcaataa
     3181 aatttctaat tcctaaaacc aaaatccagt actaaaatcc agatcgctga cctaataact
     3241 tcgtatagca tacattatac gaagttatat taagggttga atgaaaacta atctttttct
     3301 ctttctcatc ttttcacttc tcctatcatt atcctcggcc gaattcggag gtatggtgag
     3361 caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt
     3421 aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct
     3481 gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac
     3541 caccctgacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga agcagcacga
     3601 cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga
     3661 cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg
     3721 catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga
     3781 gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa
     3841 ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta
     3901 ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag
     3961 cacccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga
     4021 gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagg gtggccatga
     4081 tgagctttaa gcttgctctc aagatcaaag gcttaaaaag ctggggtttt atgaatggga
     4141 tcaaagtttc tttttttctt ttatatttgc ttctccattt gtttgtttca tttccctttt
     4201 tgttttcgtt tctatgatgc acttgtgtgt gacaaactct ctgggttttt acttacgtct
     4261 gcgtttcaaa aaaaaaaacc gctttcgttt tgcgttttag tcccattgtt ttgtagctct
     4321 gagtgatcga attgatgcct ctttattcct tttgttccct ataatttctt tcaaaactca
     4381 gaagaaaaac cttgaaactc tttgcaatgt taatataagt attgtataag atttttattg
     4441 atttggttat tagtcttact tttgctacct ccatcttcac ttggaactga tattctgaat
     4501 agttaaagcg ttacatgtgt tccattcaca aatgaactta aactagcaca aagtcagata
     4561 ttttaagatc gcaccatttc gctgagctcg aattctagtg gagaatttta agatggtgca
     4621 tggaccaccc ggaccacccg ctagctgcgt cattgtgtag tactctttct gttttaaaat
     4681 aagtgtctga actttatact aactttaata taaaattgta ctaaacttga gacatttatt
     4741 ttaggacgga gggagtagta tttatttacg tttaactatt gtacttgctc gctgtaactt
     4801 taatgacaga taacctgtac tgttaatcat gctattctgg aaacggcccc cacaacccat
     4861 tcttgaatct agactagtcg cccccttgct ggaattatac aattatttat agaaaaatct
     4921 cacaaaaaaa ttattaacaa aaaaaaaagg gagaaggcgc cctaacggca ggttcgtgag
     4981 tcccctgcaa gacgatagac ccttcgaggg ttgggaacag gaaagaacga atggctcaca
     5041 gcctacctgg aaagcgaaag agaaacaaac actcgtcgat tggacagtcc tcgagattct
     5101 tcaagatcag tacctacgat gctataagta gtggctcacc ttccatcgcc ctccatccca
     5161 actcaagcca gcgtcaagca aaagcgaaca acatcctacc tacaatccga tacagattaa
     5221 tttcaacgcg agctacaaac caaagcaagc aacaccgacc aatgtccaat ttactgaccg
     5281 tacaccaaaa tttgcctgca ttaccggtcg atgcaacgag tgatgaggtt cgcaagaacc
     5341 tgatggacat gttcagggat cgccaggcgt tttctgagca tacctggaaa atgctcctgt
     5401 ccgtttgccg gtcgtgggcg gcatggtgca agttgaataa ccggaaatgg tttcccgcag
     5461 aacctgaaga tgttcgcgat tatcttctat atcttcaggt aagtttctgc ttctaccttt
     5521 gatatatata taataattat cattaattag tagtaatata atatttcaaa tatttttttc
     5581 aaaataaaag aatgtggtat atagcaattg cttttctgta gtttataagt gtgtatattt
     5641 taatttataa cttttctaat atatgaccaa aatttgttga tgtgcaggcg cgcggtctgg
     5701 cagtaaaaac tatccagcaa catttgggcc agctaaacat gcttcatcgt cggtccgggc
     5761 tgccacgacc aagtgacagc aatgctgttt cactggttat gcggcggatc cgaaaagaaa
     5821 acgttgatgc cggtgaacgt gcaaaacagg ctctagcgtt cgaacgcact gatttcgacc
     5881 aggttcgttc actcatggaa aatagcgatc gctgccagga tatacgtaat ctggcatttc
     5941 tggggattgc ttataacacc ctgttacgta tagccgaaat tgccaggatc agggttaaag
     6001 atatctcacg tactgacggt gggagaatgt taatccatat tggcagaacg aaaacgctgg
     6061 ttagcaccgc aggtgtagag aaggcactta gcctgggggt aactaaactg gtcgagcgat
     6121 ggatttccgt ttctggtgta gctgatgatc cgaataacta cctgttttgc cgggtcagaa
     6181 aaaatggtgt tgccgcgcca tctgccacca gccagctatc aactcgcgcc ctggaaggga
     6241 tttttgaagc aactcatcga ttgatttacg gcgctaagga tgactctggt cagagatacc
     6301 tggcctggtc tggacacagt gcccgtgtcg gagccgcgcg agatatggcc cgcgctggag
     6361 tttcaatacc ggagatcatg caagctggtg gctggaccaa tgtaaatatt gtcatgaact
     6421 atatccgtaa cctggatagt gaaacagggg caatggtgcg cctgctggaa gatggcgatt
     6481 aggcttatat gaagatgaag atgaaatatt tggtgtgtca aataaaaagc ttgtgtgctt
     6541 aagtttgtgt ttttttcttg gcttgttgtg ttatgaattt gtggcttttt ctaatattaa
     6601 atgaatgtaa gatctcatta taatgaataa acaaatgttt ctataatcca ttgtgaatgt
     6661 tttgttggat ctcttctgca gcatataact actgtatgtg ctatggtatg gactatggaa
     6721 tatgattaaa gataagcgct aagcttgaat tcttgcggag gtcaacatgg tggagcacga
     6781 cactctggtc tactccaaaa atgtcaaaga tacagtctca gaagatcaaa gggctattga
     6841 gacttttcaa caaaggataa tttcgggaaa cctcctcgga ttccattgcc cagctatctg
     6901 tcacttcatc gaaaggacag tagaaaagga aggtggctcc tacaaatgcc atcattgcga
     6961 taaaggaaag gctatcattc aagatctctc tgccgacagt ggtcccaaag atggaccccc
     7021 acccacgagg agcatcgtgg aaaaagaaga ggttccaacc acgtctacaa agcaagtgga
     7081 ttgatgtgac atctccactg acgtaaggga tgacgcacaa tcccactatc cttcgcaaga
     7141 cccttcctct atataaggaa gttcatttca tttggagagg acacgcaatg aaaaagcctg
     7201 aactcaccgc gacgtctgtc gagaagtttc tgatcgaaaa gttcgacagc gtgtccgacc
     7261 tgatgcagct ctcggagggc gaagaatctc gtgctttcag cttcgatgta ggagggcgtg
     7321 gatatgtcct gcgggtaaat agctgcgccg atggtttcta caaagatcgt tatgtttatc
     7381 ggcactttgc atcggccgcg ctcccgattc cggaagtgct tgacattggg gaattcagcg
     7441 agagcctgac ctattgcatc tcccgccgtg cccagggtgt cacgttgcaa gacctgcctg
     7501 aaaccgaact gcccgctgtt ctgcaggtaa atttctagtt tttctccttc attttcttgg
     7561 ttaggaccct tttctctttt tatttttttg agctttgatc tttctttaaa ctgatctatt
     7621 ttttaattga ttggttatgg tgtaaatatt acatagcttt aactgataat ctgattactt
     7681 tatttcgtgt gtctatgatg atgatgataa ctgcagccgg tcgcggaggc catggatgcg
     7741 atcgctgcgg ccgatcttag ccagacgagc gggttcggcc cattcggacc gcaaggaatc
     7801 ggtcaataca ctacatggcg tgatttcata tgcgcgattg ctgatcccca tgtgtatcac
     7861 tggcaaactg tgatggacga caccgtcagt gcgtccgtcg cgcaggctct cgatgagctg
     7921 atgctttggg ccgaggactg ccccgaagtc cggcatctcg tgcacgcgga tttcggctcc
     7981 aacaatgtcc tgacggacaa tggccgcata acagcggtca ttgactggag cgaggcgatg
     8041 ttcggggatt cccaatacga ggtcgccaac atcttcttct ggaggccgtg gttggcttgt
     8101 atggagcagc agacgcgcta cttcgagcgg aggcatccgg agcttgcagg atcgccgcgg
     8161 ctccgggcgt atatgctccg cattggtctt gaccaactct atcagagctt ggttgacggc
     8221 aatttcgatg atgcagcttg ggcgcagggt cgatgcgacg caatcgtccg atccggagcc
     8281 gggactgtcg ggcgtacaca aatcgcccgc agaagcgcgg ccgtctggac cgatggctgt
     8341 gtagaagtac tcgccgatag tggaaaccga cgccccagca ctcgtccgag ggcaaaggaa
     8401 taggcttgtc aagcagatcg ttcaaacatt tggcaataaa gtttcttaag attgaatcct
     8461 gttgccggtc ttgcgatgat tatcatataa tttctgttga attacgttaa gcatgtaata
     8521 attaacatgt aatgcatgac gttatttatg agatgggttt ttatgattag agtcccgcaa
     8581 ttatacattt aatacgcgat agaaaacaaa atatagcgcg caaactagga taaattatcg
     8641 cgcgcggtgt catctatgtt actagatcga cgctggatcc gaattcggca gggcccactg
     8701 catccacccc agtacattaa aaacgtccgc aatgtgttat taagttgtct aagcgtcaat
     8761 ttgtttacac cacaatatat cctgccacca gccagccaac agctccccga ccggcagctc
     8821 ggcacaaaat caccactcga tacaggcagc ccatcagccg tgcggctgca tgaaatcctg
     8881 gccggtttgt ctgatgccaa gctggcggcc tggccggcca gcttggccgc tgaagaaacc
     8941 gagcgccgcc gtctaaaaag gtgatgtgta tttgagtaaa acagcttgcg tcatgcggtc
     9001 gctgcgtata tgatgcgatg agtaaataaa caaatacgca aggggaacgc atgaaggtta
     9061 tcgctgtact taaccagaaa ggcgggtcag gcaagacgac catcgcaacc catctagccc
     9121 gcgccctgca actcgccggg gccgatgttc tgttagtcga ttccgatccc cagggcagtg
     9181 cccgcgattg ggcggccgtg cgggaagatc aaccgctaac cgttgtcggc atcgaccgcc
     9241 cgacgattga ccgcgacgtg aaggccatcg gccggcgcga cttcgtagtg atcgacggag
     9301 cgccccaggc ggcggacttg gctgtgtccg cgatcaaggc agccgacttc gtgctgattc
     9361 cggtgcagcc aagcccttac gacatatggg ccaccgccga cctggtggag ctggttaagc
     9421 agcgcattga ggtcacggat ggaaggctac aagcggcctt tgtcgtgtcg cgggcgatca
     9481 aaggcacgcg catcggcggt gaggttgccg aggcgctggc cgggtacgag ctgcccattc
     9541 ttgagtcccg tatcacgcag cgcgtgagct acccaggcac tgccgccgcc ggcacaaccg
     9601 ttcttgaatc agaacccgag ggcgacgctg cccgcgaggt ccaggcgctg gccgctgaaa
     9661 ttaaatcaaa actcatttga gttaatgagg taaagagaaa atgagcaaaa gcacaaacac
     9721 gctaagtgcc ggccgtccga gcgcacgcag cagcaaggct gcaacgttgg ccagcctggc
     9781 agacacgcca gccatgaagc gggtcaactt tcagttgccg gcggaggatc acaccaagct
     9841 gaagatgtac gcggtacgcc aaggcaagac cattaccgag ctgctatctg aatacatcgc
     9901 gcagctacca gagtaaatga gcaaatgaat aaatgagtag atgaatttta gcggctaaag
     9961 gaggcggcat ggaaaatcaa gaacaaccag gcaccgacgc cgtggaatgc cccatgtgtg
    10021 gaggaacggg cggttggcca ggcgtaagcg gctgggttgt ctgccggccc tgcaatggca
    10081 ctggaacccc caagcccgag gaatcggcgt gagcggtcgc aaaccatccg gcccggtaca
    10141 aatcggcgcg gcgctgggtg atgacctggt ggagaagttg aaggccgcgc aggccgccca
    10201 gcggcaacgc atcgaggcag aagcacgccc cggtgaatcg tggcaagcgg ccgctgatcg
    10261 aatccgcaaa gaatcccggc aaccgccggc agccggtgcg tcgtcgatta ggaagccgcc
    10321 caagggcgac gagcaaccag attttttcgt tccgatgctc tatgacgtgg gcacccgcga
    10381 tagtcgcagc atcatggacg tggccgtttt ccgtctgtcg aagcgtgacc gacgagctgg
    10441 cgaggtgatc cgctacgagc ttccagacgg gcacgtagag gtttccgcag ggccggccgg
    10501 catggccagt gtgtgggatt acgacctggt actgatggcg gtttcccatc taaccgaatc
    10561 catgaaccga taccgggaag ggaagggaga caagcccggc cgcgtgttcc gtccacacgt
    10621 tgcggacgta ctcaagttct gccggcgagc cgatggcgga aagcagaaag acgacctggt
    10681 agaaacctgc attcggttaa acaccacgca cgttgccatg cagcgtacga agaaggccaa
    10741 gaacggccgc ctggtgacgg tatccgaggg tgaagccttg attagccgct acaagatcgt
    10801 aaagagcgaa accgggcggc cggagtacat cgagatcgag ctagctgatt ggatgtaccg
    10861 cgagatcaca gaaggcaaga acccggacgt gctgacggtt caccccgatt actttttgat
    10921 cgatcccggc atcggccgtt ttctctaccg cctggcacgc cgcgccgcag gcaaggcaga
    10981 agccagatgg ttgttcaaga cgatctacga acgcagtggc agcgccggag agttcaagaa
    11041 gttctgtttc accgtgcgca agctgatcgg gtcaaatgac ctgccggagt acgatttgaa
    11101 ggaggaggcg gggcaggctg gcccgatcct agtcatgcgc taccgcaacc tgatcgaggg
    11161 cgaagcatcc gccggttcct aatgtacgga gcagatgcta gggcaaattg ccctagcagg
    11221 ggaaaaaggt cgaaaaagct tctttcctgt ggatagcacg tacattggga acccaaagcc
    11281 gtacattggg aaccggaacc cgtacattgg gaacccaaag ccgtacattg ggaaccggtc
    11341 acacatgtaa gtgactgata taaaagagaa aaaaggcgat ttttccgcct aaaactcttt
    11401 aaaacttatt aaaactctta aaacccgcct ggcctgtgca taactgtctg gccagcgcac
    11461 agccgaagag ctgcaaaaag cgcctaccct tcggtcgctg cgctccctac gccccgccgc
    11521 ttcgcgtcgg cctatcgcgg ccgctggccg ctcaaaaatg gctggcctac ggccaggcaa
    11581 tctaccaggg cgcggacaag ccgcgccgtc gccactcgac cgccggcgcc cacatcaagg
    11641 caccgagctc ccatcccccc aggggctgcg cccctcggcc gcgaacggcc tcaccccaaa
    11701 aatggcagcg ctggccaatt cgtgcgcgga acccctattt gtttattttt ctaaatacat
    11761 tcaaatatgt atccgctcat gagacaataa ccttgataaa tgcttcaata atattgaaaa
    11821 aggaagagta tggctaaaat gagaatatca ccggaattga aaaaactgat cgaaaaatac
    11881 cgctgcgcaa aagatacgga aggaatgtct cctgctaagg tatataagct ggtgggagaa
    11941 aatgaaaacc tatatttaaa aatgacggac agccggtata aagggaccac ctatgatgtg
    12001 gaacgggaaa aggacatgat gctatggctg gaaggaaagc tgcctgttcc aaaggtcctg
    12061 cactttgaac ggcatgatgg ctggagcaat ctgctcatga gtgaggccga tggcgtcctt
    12121 tgctcggaag agtatgaaga tgaacaaagc cctgaaaaga ttatcgagct gtatgcggag
    12181 tgcatcaggc tctttcactc catcgacata tcggattgtc cctatacgaa tagcttagac
    12241 agccgcttag ccgaattgga ttacttactg aataacgatc tggccgatgt ggattgcgaa
    12301 aactgggaag aggacactcc atttaaagat ccgcgcgagc tgtatgattt tttaaagacg
    12361 gaaaagcccg aagaggaact tgtcttttcc cacggcgacc tgggagacag caacatcttt
    12421 gtgaaagatg gcaaagtaag tggctttatt gatcttggga gaagcggcag ggcggacaag
    12481 tggtatgaca ttgccttctg cgtccggtcg atcagggagg atatcgggga agaacagtat
    12541 gtcgagctat tttttgactt actggggatc aagcctgatt gggagaaaat aaaatattat
    12601 attttactgg atgaattgtt ttagctgtca gaccaagttt actcatatat actttagatt
    12661 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
    12721 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
    12781 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
    12841 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
    12901 aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag
    12961 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
    13021 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
    13081 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
    13141 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
    13201 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
    13261 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
    13321 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
    13381 aaaaacgcca gcaacgcggc ctttttacgg ttcct
//
