LOCUS       sequence-313094-        3222 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 Golden Gate vector, pHvHSP17.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3222)
  AUTHORS   Harrington SA, Backhaus AE, Fox S, Rogers C, Borrill P, Uauy C, 
            Richardson A
  TITLE     A heat-shock inducible system for flexible gene expression in 
            cereals.
  JOURNAL   Plant Methods. 2020 Oct 14;16:137. doi: 10.1186/s13007-020-00677-3. 
            eCollection 2020.
  PUBMED    33072173
REFERENCE   2  (bases 1 to 3222)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Methods."; date: "2020-10-14"; volume: "16:137. doi"; pages: " 
            10.1186/s13007-020-00677-3. eCollection 2020"
COMMENT     Sequence Label: EC71100
FEATURES             Location/Qualifiers
     source          1..3222
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     87..109
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     101..118
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     102..118
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     129..148
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        129..147
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1021..1038)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1131..1719)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1211..1230)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        complement(2904..3008)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ttcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg
       61 attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtga
      121 gcgcgacgta atacgactca ctatagggcg aattgagtga aggccgtcaa ggccacgtgt
      181 cttgtccaga gctccactct gtggtctcag gagaatttta agatggtgca tggaccaccc
      241 ggaccacccg ctagctgcgt cattgtgtag tactctttct gttttaaaat aagtgtctga
      301 actttatact aactttaata taaaattgta ctaaacttga gacatttatt ttaggacgga
      361 gggagtagta tttatttacg tttaactatt gtacttgctc gctgtaactt taatgacaga
      421 taacctgtac tgttaatcat gctattctgg aaacggcccc cacaacccat tcttgaatct
      481 agactagtcg cccccttgct ggaattatac aattatttat agaaaaatct cacaaaaaaa
      541 ttattaacaa aaaaaaaagg gagaaggcgc cctaacggca ggttcgtgag tcccctgcaa
      601 gacgatagac ccttcgaggg ttgggaacag gaaagaacga atggctcaca gcctacctgg
      661 aaagcgaaag agaaacaaac actcgtcgat tggacagtcc tcgagattct tcaagatcag
      721 tacctacgat gctataagta gtggctcacc ttccatcgcc ctccatccca actcaagcca
      781 gcgtcaagca aaagcgaaca acatcctacc tacaatccga tacagattaa tttcaacgcg
      841 agctacaaac caaagcaagc aacaccgacc aatgtgagac cacgaagtgg gtacctggag
      901 cacaagactg gcctcatggg ccttcacttc actgcccgct ttccagtcgg gaaacctgtc
      961 gtgccagctg cattaacatg gtcatagctg tttccttgcg tattgggcgc tctccgcttc
     1021 ctcgctcact gactcgctgc gctcggtcgt tcgggtaaag cctggggtgc ctaatgagca
     1081 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     1141 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     1201 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     1261 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     1321 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     1381 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     1441 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     1501 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     1561 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     1621 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     1681 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     1741 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     1801 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     1861 agtatatatg agtaaacttg gtctgacagt tattatttac ccaccacttt ggtaatttcg
     1921 cctttcacgt aatgcacaaa ttcttccagc tgatccgcac ggctggccag acgatcttct
     1981 tcctggccca gatacgcctg acgcgcttcc agaatcaccg gctgatactg cgccggcaga
     2041 cgttccatgg cccaatccgc cgccacatct ttcggcgcaa ttttgccggt caccgcgcta
     2101 taccaaatac ggctcagggt cagcaccacg ttgcgttcat cacccgccca atccggcggg
     2161 ctgttccaca gggtcagggt ttcgttcagc gcttcaaaca ggtcctgttc cggcaccgga
     2221 tcaaacagtt cttccgctgc cggaccaacc agcgccacgc tatgttcacg cgctttggtc
     2281 agcagaatgg ccagatcgat atcaatggtc gccggttcaa aaatgccggc cagaatatcg
     2341 ttacgctgcc attcgccaaa ctgcagctca cgtttcgccg gataacgcca cggaataatg
     2401 tcatcatgca ccacgatggt cacttccacc gcacgcagaa tttcgctttc acccgggctc
     2461 gcgctggttt ccagcagatc gttaatcagc gcacgacggg tggtttcatc cagacgcacg
     2521 gtcacggtca ccagcagatc aatatcgcta tgcggtttca ggccgccatc caccgcgctg
     2581 ccatacagat gcacggccag cagggtcggt tccagatgac gttcaatcac gcccaccact
     2641 tcagacagct gggtgctcac ttccgcaatc accgcttcac gcataatgtt cagtttggtt
     2701 ttcggacggc tgccctgctg ggtcacatcg ttgctgctcc acagcatcag gcaacggccc
     2761 accgcataac gcgcctgctg tttgctcgca cgcggcatgc tctgcacgcc aaaaaagcaa
     2821 tcatagcacg ccataaacac cgccaccgca ccattaccgc cgctacgttc ggtcagggta
     2881 cggctccaat tacggctacg catactcttc ctttttcaat attattgaag catttatcag
     2941 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     3001 gttccgcgca catttccccg aaaagtgcca cctaaattgt aagcgttaat attttgttaa
     3061 aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca
     3121 aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtggccgc tacagggcgc
     3181 tcccattcgc cattcaggct gcgcaactgt tgggaagggc gt
//
