LOCUS       sequence-313109-       10541 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 1, Position 3 Golden Gate vector. 
            ZmUbi-5'UTR:loxP-GUS-nosT-loxP-NAM-B1-nosT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10541)
  AUTHORS   Harrington SA, Backhaus AE, Fox S, Rogers C, Borrill P, Uauy C, 
            Richardson A
  TITLE     A heat-shock inducible system for flexible gene expression in 
            cereals.
  JOURNAL   Plant Methods. 2020 Oct 14;16:137. doi: 10.1186/s13007-020-00677-3. 
            eCollection 2020.
  PUBMED    33072173
REFERENCE   2  (bases 1 to 10541)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Methods."; date: "2020-10-14"; volume: "16:137. doi"; pages: " 
            10.1186/s13007-020-00677-3. eCollection 2020"
COMMENT     Sequence Label: pNB1-LOX
FEATURES             Location/Qualifiers
     source          1..10541
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1332..1356
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     rep_origin      1448..2079
                     /label=oriV
                     /note="incP origin of replication"
     promoter        2166..2270
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2271..2339,2340..3131)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2489..2508)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3302..3890
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3791..3810
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             4226..5374
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     misc_feature    5530..5554
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     terminator      complement(5602..5854)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     protein_bind    7395..7428
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     terminator      complement(7441..7693)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     protein_bind    9734..9767
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        complement(join(9779..10541,1..1228))
                     /label=Ubi promoter
                     /note="maize polyubiquitin gene promoter"
ORIGIN
        1 gatcccgtct gcggaacggc tagagccatc ccaggattcc ccaaagagaa acactggcaa
       61 gttagcaatc agaacgtgtc tgacgtacag gtcgcatccg tgtacgaacg ctagcagcac
      121 ggatctaaca caaacacgga tctaacacaa acatgaacag aagtagaact accgggccct
      181 aaccatggac cggaacgccg atctagagaa ggtagagagg gggggggggg aggacgagcg
      241 gcgtaccttg aagcggaggt gccgacgggt ggatttgggg gagatctggt tgtgtgtgtg
      301 tgcgctccga acaacacgag gttggggaaa gagggtgtgg agggggtgtc tatttattac
      361 ggcgggcgag gaagggaaag cgaaggagcg gtgggaaagg aatcccccgt agctgccgtg
      421 ccgtgagagg aggaggaggc cgcctgccgt gccggctcac gtctgccgct ccgccacgca
      481 atttctggat gccgacagcg gagcaagtcc aacggtggag cggaactctc gagaggggtc
      541 cagaggcagc gacagagatg ccgtgccgtc tgcttcgctt ggcccgacgc gacgctgctg
      601 gttcgctggt tggtgtccgt tagactcgtc gacggcgttt aacaggctgg cattatctac
      661 tcgaaacaag aaaaatgttt ccttagtttt tttaatttct taaagggtat ttgtttaatt
      721 tttagtcact ttattttatt ctattttata tctaaattat taaataaaaa aactaaaata
      781 gagttttagt tttcttaatt tagaggctaa aatagaataa aatagatgta ctaaaaaaat
      841 tagtctataa aaaccattaa ccctaaaccc taaatggatg tactaataaa atggatgaag
      901 tattatatag gtgaagctat ttgcaaaaaa aaaggagaac acatgcacac taaaaagata
      961 aaactgtaga gtcctgttgt caaaatactc aattgtcctt tagaccatgt ctaactgttc
     1021 atttatatga ttctctaaaa cactgatatt attgtagtac tatagattat attattcgta
     1081 gagtaaagtt taaatatatg tataaagata gataaactgc acttcaaaca agtgtgacaa
     1141 aaaaaatatg tggtaatttt ttataactta gacatgcaat gctcattatc tctagagagg
     1201 ggcacgaccg ggtcacgctg cacctccgag ctcgaattct agtttgtctt cacagagtgg
     1261 ggcccactgc atccacccca gtacattaaa aacgtccgca atgtgttatt aagttgtcta
     1321 agcgtcaatt tgtttacacc acaatatatc ctgccaccag ccagccaaca gctccccgac
     1381 cggcagctcg gcacaaaatc accactcgat acaggcagcc catcagtcag atcaggatct
     1441 cctttgcgac gctcaccggg ctggttgccc tcgccgctgg gctggcggcc gtctatggcc
     1501 ctgcaaacgc gccagaaacg ccgtcgaagc cgtgtgcgag acaccgcggc cgccggcgtt
     1561 gtggatacct cgcggaaaac ttggccctca ctgacagatg aggggcggac gttgacactt
     1621 gaggggccga ctcacccggc gcggcgttga cagatgaggg gcaggctcga tttcggccgg
     1681 cgacgtggag ctggccagcc tcgcaaatcg gcgaaaacgc ctgattttac gcgagtttcc
     1741 cacagatgat gtggacaagc ctggggataa gtgccctgcg gtattgacac ttgaggggcg
     1801 cgactactga cagatgaggg gcgcgatcct tgacacttga ggggcagagt gctgacagat
     1861 gaggggcgca cctattgaca tttgaggggc tgtccacagg cagaaaatcc agcatttgca
     1921 agggtttccg cccgtttttc ggccaccgct aacctgtctt ttaacctgct tttaaaccaa
     1981 tatttataaa ccttgttttt aaccagggct gcgccctgtg cgcgtgaccg cgcacgccga
     2041 aggggggtgc ccccccttct cgaaccctcc cggcccgcta acgcgggcct cccatccccc
     2101 caggggctgc gcccctcggc cgcgaacggc ctcaccccaa aaatggcagc gctggccaat
     2161 tcgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     2221 tgagacaata accttgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     2281 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     2341 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
     2401 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
     2461 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
     2521 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
     2581 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
     2641 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
     2701 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
     2761 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
     2821 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
     2881 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
     2941 cggctggctg gtttattgct gataaatctg gagccggtga gcgtggttct cgcggtatca
     3001 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     3061 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     3121 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
     3181 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     3241 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     3301 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     3361 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     3421 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
     3481 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     3541 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     3601 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     3661 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     3721 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     3781 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     3841 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     3901 acgcggcctt tttacggttc ctggcagatc ctagatgtgg cgcaacgatg ccggcgacaa
     3961 gcaggagcgc accgacttct tccgcatcaa gtgttttggc tctcaggccg aggcccacgg
     4021 caagtatttg ggcaaggggt cgctggtatt cgtgcagggc aagattcgga ataccaagta
     4081 cgagaaggac ggccagacgg tctacgggac cgacttcatt gccgataagg tggattatct
     4141 ggacaccaag gcaccaggcg ggtcaaatca ggaataaggg cacattgccc cggcgtgagt
     4201 cggggcaatc ccgcaaggag ggtgaatgaa tcggacgttt gaccggaagg catacaggca
     4261 agaactgatc gacgcggggt tttccgccga ggatgccgaa accatcgcaa gccgcaccgt
     4321 catgcgtgcg ccccgcgaaa ccttccagtc cgtcggctcg atggtccagc aagctacggc
     4381 caagatcgag cgcgacagcg tgcaactggc tccccctgcc ctgcccgcgc catcggccgc
     4441 cgtggagcgt tcgcgtcgtc ttgaacagga ggcggcaggt ttggcgaagt cgatgaccat
     4501 cgacacgcga ggaactatga cgaccaagaa gcgaaaaacc gccggcgagg acctggcaaa
     4561 acaggtcagc gaggccaagc aggccgcgtt gctgaaacac acgaagcagc agatcaagga
     4621 aatgcagctt tccttgttcg atattgcgcc gtggccggac acgatgcgag cgatgccaaa
     4681 cgacacggcc cgctctgccc tgttcaccac gcgcaacaag aaaatcccgc gcgaggcgct
     4741 gcaaaacaag gtcattttcc acgtcaacaa ggacgtgaag atcacctaca ccggcgtcga
     4801 gctgcgggcc gacgatgacg aactggtgtg gcagcaggtg ttggagtacg cgaagcgcac
     4861 ccctatcggc gagccgatca ccttcacgtt ctacgagctt tgccaggacc tgggctggtc
     4921 gatcaatggc cggtattaca cgaaggccga ggaatgcctg tcgcgcctac aggcgacggc
     4981 gatgggcttc acgtccgacc gcgttgggca cctggaatcg gtgtcgctgc tgcaccgctt
     5041 ccgcgtcctg gaccgtggca agaaaacgtc ccgttgccag gtcctgatcg acgaggaaat
     5101 cgtcgtgctg tttgctggcg accactacac gaaattcata tgggagaagt accgcaagct
     5161 gtcgccgacg gcccgacgga tgttcgacta tttcagctcg caccgggagc cgtacccgct
     5221 caagctggaa accttccgcc tcatgtgcgg atcggattcc acccgcgtga agaagtggcg
     5281 cgagcaggtc ggcgaagcct gcgaagagtt gcgaggcagc ggcctggtgg aacacgcctg
     5341 ggtcaatgat gacctggtgc attgcaaacg ctagggcctt gtggggtcag ttccggctgg
     5401 gggttcagca gccagcgcct gatctgggga accctgtggt tggcatgcac atacaaatgg
     5461 acgaacggat aaaccttttc acgccctttt aaatatccga ttattctaat aaacgctctt
     5521 ttctcttagg tttacccgcc aatatatcct gtcaaacact gatagtttaa accacttcgt
     5581 gcagaagaca agtaaagcgt cgatctagta acatagatga caccgcgcgc gataatttat
     5641 cctagtttgc gcgctatatt ttgttttcta tcgcgtatta aatgtataat tgcgggactc
     5701 taatcataaa aacccatctc ataaataacg tcatgcatta catgttaatt attacatgct
     5761 taacgtaatt caacagaaat tatatgataa tcatcgcaag accggcaaca ggattcaatc
     5821 ttaagaaact ttattgccaa atgtttgaac gatctgcttg acaagctcag ggattccagt
     5881 tcacgccgga tatttgaacg ggatgctgga atccagaggt ggcggcggcg gcttctggca
     5941 ccagcggagc gccgaggatg acatgctgct gatggtacgt cccgtcttgc acgggaagat
     6001 aggtagacat ggcgttcacc gcattgcctc caccgttgtc cactgcgcca gccatgtcgg
     6061 ccgtcgatga cagcgacata ttgccgacgt ttctgctcct gttaaaccca ggaaaattcc
     6121 ttgcctgtgg gaggatgccg actggtgacg catcgagcca gttgaacggg gtcgacgatg
     6181 acggggcgag aaactgtttg gtcggagccg ggcttgccct cgccgccgct gctaggtggc
     6241 tcagcgaggt ggcgcccgcc gagaggccgg cgtcgtctgc tgtgaacagg ccgtccagga
     6301 aatggctgtc ctgatgatgg agcagtgaag gtgaagcgta gttgctgcca tgcgcacctg
     6361 caccggtcat gcccgccgtg gcatagagcg ggtacgcggt gaccgcgtcc tccacggagt
     6421 cctcgcactc cgtgttcctc tgctgatcgc cggccgcggc cttgttgatc ttcttgtaga
     6481 tgcggcacag cacccagtcg tccaacttca tcaaccatca acaataccct aattaagctg
     6541 gagagcaatt aaatactgct agaccgtgcg atcgacacgt gtacgtaccc tgagagaggc
     6601 agcagccctg ctcccgccgg tcaccggcgg cggtcggttg gtggcggtgg tggagccaga
     6661 tgcgtcggtg aggcggtact cgtgcatgat ccagttggtt ttgaggccct tgggcggctt
     6721 cccgcggtag aacacgagcg ccttcttgac gccgagcttc tcccggacca ggccgcaccc
     6781 cgtccccgag gccaggatag gcttgtccgt gccggtggcc ttccagtacc ccgacgtcgc
     6841 cgcccggttc ggccgcgcgc cgttggggta cttgcggtcg cgcgggctga agaagtacca
     6901 ctcctgctcc ccgatggtcg ccttctctga tggatccgga tcccgagcgc atgaacaagc
     6961 acgacaggat cacatacata aaccatctac acacatgtgt actggaagaa tataaaaata
     7021 ctacttgtgc acaggagtat aagcatgaga tggatggatt attcgattat ttaattgcac
     7081 ctaaaatttg cggcgcttga taaagtaagc acggccggcc gacgagatag acataacata
     7141 ccggggagct cccatgggtc gaacttgtag agatccacct cggcgatgat gttgacgggg
     7201 agcggcgcct tgtcggcctt cttcttgagg tagtgcacca ccagctcctc gtccgtcggg
     7261 tggaaccgga agcccggcgg gagctccggc gccgagcccc gctgcggagg cggcggctga
     7321 tgctgatggt gataccgcgt tgctttttgc gccgagccgg aagatgagtc ggagctgccc
     7381 attcaaccct taatataact tcgtataatg tatgctatac gaagttatta ggtcagcgtc
     7441 gatctagtaa catagatgac accgcgcgcg ataatttatc ctagtttgcg cgctatattt
     7501 tgttttctat cgcgtattaa atgtataatt gcgggactct aatcataaaa acccatctca
     7561 taaataacgt catgcattac atgttaatta ttacatgctt aacgtaattc aacagaaatt
     7621 atatgataat catcgcaaga ccggcaacag gattcaatct taagaaactt tattgccaaa
     7681 tgtttgaacg atctgcttga caagctcatt gtttgcctcc ctgctgcggt ttttcaccga
     7741 agttcatgcc agtccagcgt ttttgcagca gaaaagccgc cgacttcggt ttgcggtcgc
     7801 gagtgaagat ccctttcttg ttaccgccaa cgcgcaatat gccttgcgag gtcgcaaaat
     7861 cggcgaaatt ccatacctgt tcaccgacga cggcgctgac gcgatcaaag acgcggtgat
     7921 acatatccag ccatgcacac tgatactctt cactccacat gtcggtgtac attgagtgca
     7981 gcccggctaa cgtatccacg ccgtattcgg tgatgataat cggctgatgc agtttctcct
     8041 gccaggccag aagttctttt tccagtacct tctctgccgt ttccaaatcg ccgctttgga
     8101 cataccatcc gtaataacgg ttcaggcaca gcacatcaaa gagatcgctg atggtatcgg
     8161 tgtgagcgtc gcagaacatt acattgacgc aggtgatcgg acgcgtcggg tcgagtttac
     8221 gcgttgcttc cgccagtggc gcgaaatatt cccgtgcacc ttgcggacgg gtatccggtt
     8281 cgttggcaat actccacatc accacgcttg ggtggttttt gtcacgcgct atcagctctt
     8341 taatcgcctg taagtgcgct tgctgagttt ccccgttgac tgcctcttcg ctgtacagtt
     8401 ctttcggctt gttgcccgct tcgaaaccaa tgcctaaaga gaggttaaag ccgacagcag
     8461 cagtttcatc aatcaccacg atgccatgtt catctgccca gtcgagcatc tcttcagcgt
     8521 aagggtaatg cgaggtacgg taggagttgg ccccaatcca gtccattaat gcgtggtcgt
     8581 gcaccatcag cacgttatcg aatcctttgc cacgcaagtc cgcatcttca tgacgaccaa
     8641 agccagtaaa gtagaacggt ttgtggttaa tcaggaactg ttcgcccttc actgccactg
     8701 accggatgcc gacgcgaagc gggtagatat cacactctgt ctggcttttg gctgtgacgc
     8761 acagttcata gagataacct tcacccggtt gccagaggtg cggattcacc acttgcaaag
     8821 tcccgctagt gccttgtcca gttgcaacca cctgttgatc cgcatcacgc agttcaacgc
     8881 tgacatcacc attggccacc acctgtaagg ggaaaaaaat tgacaaaaag caaaggtcaa
     8941 gcaaatgtat ggaaatataa aatttcacac agtatctctt aagttacacg ttgaagcatg
     9001 aagtacctgc cagtcaacag acgcgtggtt acagtcttgc gcgacatgcg tcaccacggt
     9061 gatatcgtcc acccaggtgt tcggcgtggt gtagagcatt acgctgcgat ggattccggc
     9121 atagttaaag aaatcatgga agtaagactg ctttttcttg ccgttttcgt cggtaatcac
     9181 cattcccggc gggatagtct gccagttcag ttcgttgttc acacaaacgg tgatacgtac
     9241 acttttcccg gcaataacat acggcgtgac atcggcttca aatggcgtat agccgccctg
     9301 atgctccatc acttcctgat tattgaccca cactttgccg taatgagtga ccgcatcgaa
     9361 acgcagcacg atacgctggc ctgcccaacc tgaaaaaaga ttattagcgt cagtacaaat
     9421 agtatacttc aaaaaattaa aaatacagac caaaaaagag agagtaagac ttacctttcg
     9481 gtataaagac ttcgcgctga taccagacgt tgcccgcata attacgaata tctgcatcgg
     9541 cgaactgatc gttaaaactg cctggcacag caattgcccg gctttcttgt aacgcgcttt
     9601 cccaccaacg ctgatcaatt ccacagtttt cgcgatccag actgaatgcc cacaggccgt
     9661 cgagtttttt gatttcacgg gttggggttt ctacaggacg taacataagg gactgaccca
     9721 ttcaaccctt aatataactt cgtataatgt atgctatacg aagttattag gtcagtacct
     9781 gcagaagtaa caccaaacaa cagggtgagc atcgacaaaa gaaacagtac caagcaaata
     9841 aatagcgtat gaaggcaggg ctaaaaaaat ccacatatag ctgctgcata tgccatcatc
     9901 caagtatatc aagatcaaaa taattataaa acatacttgt ttattataat agataggtac
     9961 tcaaggttag agcatatgaa tagatgctgc atatgccatc atgtatatgc atcagtaaaa
    10021 cccacatcaa catgtatacc tatcctagat cgatatttcc atccatctta aactcgtaac
    10081 tatgaagatg tatgacacac acatacagtt ccaaaattaa taaatacacc aggtagtttg
    10141 aaacagtatt ctactccgat ctagaacgaa tgaacgaccg cccaaccaca ccacatcatc
    10201 acaaccaagc gaacaaaaag catctctgta tatgcatcag taaaacccgc atcaacatgt
    10261 atacctatcc tagatcgata tttccatcca tcatcttcaa ttcgtaacta tgaatatgta
    10321 tggcacacac atacagatcc aaaattaata aatccaccag gtagtttgaa acagaattct
    10381 actccgatct agaacgaccg cccaaccaga ccacatcatc acaaccaaga caaaaaaaag
    10441 catgaaaaga tgacccgaca aacaagtgca cggcatatat tgaaataaag gaaaagggca
    10501 aaccaaaccc tatgcaacga aacaaaaaaa atcatgaaat c
//
