LOCUS       sequence-313112-       11560 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  parental, all-in-one CRISPRi vector for B. burgdorferi, parental for
            gRNA cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11560)
  AUTHORS   Takacs CN, Scott M, Chang Y, Kloos ZA, Irnov I, Rosa PA, Liu J, 
            Jacobs-Wagner C
  TITLE     A CRISPR interference platform for selective downregulation of gene 
            expression in Borrelia burgdorferi.
  JOURNAL   Appl Environ Microbiol. 2020 Nov 30. pii: AEM.02519-20. doi: 
            10.1128/AEM.02519-20.
  PUBMED    33257311
REFERENCE   2  (bases 1 to 11560)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Environ Microbiol. 2020 Nov 30. pii: AEM.02519-20. doi: 
            10.1128/AEM.02519-20."
COMMENT     Sequence Label: pBbdCas9H(RBSmut)_arr2
FEATURES             Location/Qualifiers
     source          1..11560
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(160..174,175..180,181..197,198..204)
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA 
                     polymerase, with embedded lac operator"
     protein_bind    180..196
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    212..228
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             join(265..291,292..294,295..2781,2782..2784,2785..4371)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TILERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             4558..5577
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV
                     LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET
                     ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV
                     MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD
                     SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF
                     DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"
     rep_origin      5700..6288
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6189..6208
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgcaaatagc aatagcatta aaaataatta ttattattta tttctttgat taaaatcaaa
       61 agcctttaat gtattttctt gatctttcat gcgcttaacg gtaaaatcca aggtattaag
      121 ttcaccttcc acttttctct ctctatcttc tctagaaaat cataaaaaat ttatttgctt
      181 tgtgagcgga taacaattat aatagattca attgtgagcg gataacaatt tcacacagaa
      241 ttcattaaag aagagaaatt acatatggat aagaaatact caataggctt agctatcggc
      301 acaaatagcg tcggatgggc ggtgatcact gatgaatata aggttccgtc taaaaagttc
      361 aaggttctgg gaaatacaga ccgccacagt atcaaaaaaa atcttatagg ggctctttta
      421 tttgacagtg gagagacagc ggaagcgact cgtctcaaac ggacagctcg tagaaggtat
      481 acacgtcgga agaatcgtat ttgttatcta caggagattt tttcaaatga gatggcgaaa
      541 gtagatgata gtttctttca tcgacttgaa gagtcttttt tggtggaaga agacaagaag
      601 catgaacgtc atcctatttt tggaaatata gtagatgaag ttgcttatca tgagaaatat
      661 ccaactatct atcatctgcg aaaaaaattg gtagattcta ctgataaagc ggatttgcgc
      721 ttaatctatt tggccttagc gcatatgatt aagtttcgtg gtcatttttt gattgaggga
      781 gatttaaatc ctgataatag tgatgtggac aaactattta tccagttggt acaaacctac
      841 aatcaattat ttgaagaaaa ccctattaac gcaagtggag tagatgctaa agcgattctt
      901 tctgcacgat tgagtaaatc aagacgatta gaaaatctca ttgctcagct ccccggtgag
      961 aagaaaaatg gcttatttgg gaatctcatt gctttgtcat tgggtttgac ccctaatttt
     1021 aaatcaaatt ttgatttggc agaagatgct aaattacagc tttcaaaaga tacttacgat
     1081 gatgatttag ataatttatt ggcgcaaatt ggagatcaat atgctgattt gtttttggca
     1141 gctaagaatt tatcagatgc tattttactt tcagatatcc taagagtaaa tactgaaata
     1201 actaaggctc ccctatcagc ttcaatgatt aaacgctacg atgaacatca tcaagacttg
     1261 actcttttaa aagctttagt tcgacaacaa cttccagaaa agtataaaga aatctttttt
     1321 gatcaatcaa aaaacggata tgcaggttat attgatgggg gagctagcca agaagaattt
     1381 tataaattta tcaaaccaat tttagaaaaa atggatggta ctgaggaatt attggtgaaa
     1441 ctaaatcgtg aagatttgct gcgcaagcaa cggacctttg acaacggctc tattccccat
     1501 caaattcact tgggtgagct gcatgctatt ttgagaagac aagaagactt ttatccattt
     1561 ttaaaagaca atcgtgagaa gattgaaaaa atcttgactt ttcgaattcc ttattatgtt
     1621 ggtccattgg cgcgtggcaa tagtcgtttt gcatggatga ctcggaagtc tgaagaaaca
     1681 attaccccat ggaattttga agaagttgtc gataaaggtg cttcagctca atcatttatt
     1741 gaacgcatga caaactttga taaaaatctt ccaaatgaaa aagtactacc aaaacatagt
     1801 ttgctttatg agtattttac ggtttataac gaattgacaa aggtcaaata tgttactgaa
     1861 ggaatgcgaa aaccagcatt tctttcaggt gaacagaaga aagccattgt tgatttactc
     1921 ttcaaaacaa atcgaaaagt aaccgttaag caattaaaag aagattattt caaaaaaata
     1981 gaatgttttg atagtgttga aatttcagga gttgaagata gatttaatgc ttcattaggt
     2041 acctaccatg atttgctaaa aattattaaa gataaagatt ttttggataa tgaagaaaat
     2101 gaagatatct tagaggatat tgttttaaca ttgaccttat ttgaagatag ggagatgatt
     2161 gaggaaagac ttaaaacata tgctcacctc tttgatgata aggtgatgaa acagcttaaa
     2221 cgtcgccgtt atactggttg gggacgtttg tctcgaaaat tgattaatgg tattagggat
     2281 aagcaatctg gcaaaacaat attagatttt ttgaaatcag atggttttgc caatcgcaat
     2341 tttatgcagc tgatccatga tgatagtttg acatttaaag aagacattca aaaagcacaa
     2401 gtgtctggac aaggcgatag tttacatgaa catattgcaa atttagctgg tagccctgct
     2461 attaaaaaag gtattttaca gactgtaaaa gttgttgatg aattggtcaa agtaatgggg
     2521 cggcataagc cagaaaatat cgttattgaa atggcacgtg aaaatcagac aactcaaaag
     2581 ggccagaaaa attcgcgaga gcgtatgaaa cgaatcgaag aaggtatcaa agaattagga
     2641 agtcagattc ttaaagagca tcctgttgaa aatactcaat tgcaaaatga aaagctctat
     2701 ctctattatc tccaaaatgg aagagacatg tatgtggacc aagaattaga tattaatcgt
     2761 ttaagtgatt atgatgtcga tgccattgtt ccacaaagtt tccttaaaga cgattcaata
     2821 gacaataagg tcttaacgcg ttctgataaa aatcgtggta aatcggataa cgttccaagt
     2881 gaagaagtag tcaaaaagat gaaaaactat tggagacaac ttctaaacgc caagttaatc
     2941 actcaacgta agtttgataa tttaacgaaa gctgaacgtg gaggtttgag tgaacttgat
     3001 aaagctggtt ttatcaaacg ccaattggtt gaaactcgcc aaatcactaa gcatgtggca
     3061 caaattttgg atagtcgcat gaatactaaa tacgatgaaa atgataaact tattcgagag
     3121 gttaaagtga ttaccttaaa atctaaatta gtttctgact tccgaaaaga tttccaattc
     3181 tataaagtac gtgagattaa caattaccat catgcccatg atgcgtatct aaatgccgtc
     3241 gttggaactg ctttgattaa gaaatatcca aaacttgaat cggagtttgt ctatggtgat
     3301 tataaagttt atgatgttcg taaaatgatt gctaagtctg agcaagaaat aggcaaagca
     3361 accgcaaaat atttctttta ctctaatatc atgaacttct tcaaaacaga aattacactt
     3421 gcaaatggag agattcgcaa acgccctcta atcgaaacta atggggaaac tggagaaatt
     3481 gtctgggata aagggcgaga ttttgccaca gtgcgcaaag tattgtccat gccccaagtc
     3541 aatattgtca agaaaacaga agtccagaca ggcggattct ccaaggagtc aattttacca
     3601 aaaagaaatt cggacaagct tattgctcgt aaaaaagact gggatccaaa aaaatatggt
     3661 ggttttgata gtccaacggt agcttattca gtcctagtgg ttgctaaggt ggaaaaaggg
     3721 aaatcgaaga agttaaaatc cgttaaagag ttactaggga tcacaattct ggaaagaagt
     3781 tcctttgaaa aaaatccgat tgacttttta gaagctaaag gatataagga agttaaaaaa
     3841 gacttaatca ttaaactacc taaatatagt ctttttgagt tagaaaacgg tcgtaaacgg
     3901 atgctggcta gtgccggaga attacaaaaa ggaaatgagc tggctctgcc aagcaaatat
     3961 gtgaattttt tatatttagc tagtcattat gaaaagttga agggtagtcc agaagataac
     4021 gaacaaaaac aattgtttgt ggagcagcat aagcattatt tagatgagat tattgagcaa
     4081 atcagtgaat tttctaagcg tgttatttta gcagatgcca atttagataa agttcttagt
     4141 gcatataaca aacatagaga caaaccaata cgtgaacaag cagaaaatat tattcattta
     4201 tttacgttga cgaatcttgg agctcccgct gcttttaaat attttgatac aacaattgat
     4261 cgtaaacgat atacgtctac aaaagaagtt ttagatgcca ctcttatcca tcaatccatc
     4321 actggtcttt atgaaacacg cattgatttg agtcagctag gaggtgacta ataaaagctt
     4381 gatcagatcc cagctttttt tgaagtgcct ggcagtaagt tgaaaaaatt tgaaataaac
     4441 ttggactatg ttaataaaaa aatacaaatt aacgaacaaa aagatttgaa ttattatata
     4501 atttagttaa aagcaatttt aaatgaggga ggtttccatc atatggatag aagtggtaaa
     4561 cctgaattaa ctgctactag tgttgaaaaa tttttaattg aaaaatttga tagtgttagt
     4621 gatttaatgc aattaagtga aggtgaagaa agtagagctt ttagttttga tgttggtggt
     4681 agaggttatg ttttaagagt taatagttgt gctgatggtt tttataaaga tagatatgtt
     4741 tatagacatt ttgctagtgc tgctttacct attcctgaag ttttagatat tggtgaattt
     4801 agtgaaagtt taacttattg tattagtaga agagcacaag gtgttacttt acaagattta
     4861 cctgaaactg aattacctgc tgttttacaa cctgttgctg aagctatgga tgctattgcg
     4921 gcggctgatc tcagtcaaac ttcgggtttt ggtccttttg gtcctcaagg tattggtcaa
     4981 tatactactt ggagagattt tatttgtgct attgctgatc ctcatgttta tcattggcaa
     5041 actgttatgg atgatactgt tagtgctagt gttgctcaag cattagatga attaatgtta
     5101 tgggctgaag attgtcctga agttagacat ttagttcatg ctgattttgg tagtaataat
     5161 gttttaactg ataatggtag aattactgct gttattgatt ggagtgaagc tatgtttggt
     5221 gatagtcaat atgaagttgc taatattttt ttttggagac cttggttagc ttgtatggaa
     5281 caacaaacta gatattttga aagaagacat cctgaattag ctggtagtcc tagattaaga
     5341 gcttatatgt taagaattgg tttagatcaa ttatatcaaa gtttagttga tggtaatttt
     5401 gatgatgctg cttgggctca aggtagatgt gatgctattg ttagaagtgg tgctggtact
     5461 gttggtagaa ctcaaattgc tagaagaagt gctgctgttt ggactgatgg ttgtgttgaa
     5521 gttttagctg atagtggtaa tagaagacct agtactagac ctagagctaa agaataatga
     5581 cgtcgccgga gcggtcgagt tctggaccga ccggctcggg ttctcatgac caaaatccct
     5641 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     5701 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     5761 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     5821 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     5881 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     5941 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     6001 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     6061 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     6121 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     6181 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     6241 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     6301 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgca
     6361 ctagatcccc ggcaagttca tccttagggt tttcatatct cttaaaaagt caatttctgc
     6421 ttgattaatt tcttgtggat tattgttttt gcggtttttc attttttatc tccttgagta
     6481 ttgaattttt tcttatatat aaatatatag caaaaactat ttttgccaac ttttttacaa
     6541 aaaatttttt attaaaacac ttagggcttt gctaaattct cttttaaaag aacttagtaa
     6601 agccctatgg atttaagaac tgattatact tacgtagtaa aaatactaca gatattaata
     6661 gtatagacca tatcaaaatc ataatcaatt tatatttatc taaaattgta gtccatacaa
     6721 aatacaaaaa aattaggttt ttctaaattc tattttaaaa gaacttagta acacctaatc
     6781 tctaaattac ttacaaaatt ctaaaattag atttttagtt cttcatattt ctttaaaagt
     6841 tttttaagaa aatctttttg attttcaaaa atttcatgca tcataaaact agtaaatttg
     6901 gatttttttt tgtaaaaatc ataactttct tgggttttaa gttgaaatct taatggtttt
     6961 attggatttt gcctagattt ttttaacgcc ggactttctt tatcttttat aaaattataa
     7021 gaatttttaa taccattttc tattaaataa tcttctttta taagcccctc ttgcagtgca
     7081 gttgctaatc ttaaatactt atacgcttga gtttttgcta catcaaagtc ttttataaaa
     7141 tcaagaaaag ttttataacc atcaaattta taatattgat tatcttttat ttcttttaaa
     7201 attttaatat tttccaccct gtggaatatt tcttttttga aattgtcatt taacttttcc
     7261 tttagtgtct tgtaataaga aattttactt tcattaatca ttaaattttg ttcaacataa
     7321 gtatctatct tttttgttaa aactttaaca acacgttttt tttccataaa aattttcctt
     7381 tatttttgca acattttcct taattgatat aattgttact attttagatt ttttaatatc
     7441 cacttaagaa ttaatccctc actagaatat tttttattat agaattcata tacatgtttt
     7501 tctaaatcta taaatttatc aacaattgct ttattatagg gtgtatctat cttttctttt
     7561 cttaataatc gtctcatatt ctttaaataa caacaaacac tgccggtttt aaacctaaat
     7621 tctatgtaat atatttttga aaaagtatat acattttcaa ccccgtttat ttcatacttt
     7681 acaaaaacat tatttattgg ttttctataa ccatagtaaa tgcctaaaaa tttgtccccg
     7741 tctttcatag gatatagata aagtttttga atctctgatt tatctaataa tttttcaaaa
     7801 aaaattatac accgcttttg tttgttacta actactctat gcaatgcctt taatattttt
     7861 gtgtgatata tatttttatc accttttttt tctttcagca ggaagatact ttcttttgga
     7921 aatttaattt tttgttcatt tattcttgca gccttaagtc cgattttcat atttaatcct
     7981 tatatagcct ttctatttaa ttcttttaaa ctcaataaac ttttttggtt tttcattagt
     8041 tctaataatt cgcaataata tgtgcaaaac actttattgt attctatttt cttttggtta
     8101 ttcaaatatt cttttataat tggctttaga agttcaatat ttacttcttt ttttagttgt
     8161 tcaataagga tattgaaaat gtttgttttt aaatcttgat aattttgttg agaattttct
     8221 ttttttcttt caattgactt ctctaattta cgctttatat tgtttaaatc gctatattta
     8281 tgactttcaa taataaaatg aggcttaaat ttgtaatttt cgtatatttt ttggaaactt
     8341 gtttctaatt gtttagaatt atattcattt ttttcaaatt ctttttgagt gttgcataga
     8401 atttccttta atttttcttg tttttctttc atacaagatt tagtaaaatg tatattttta
     8461 ttttttagta atttaatttc tattattttt atgattttaa gtattttgat tatattatct
     8521 ttatcaatat ttaaattgaa taaaattgta agaaaaattg aaagaatttt tttacataaa
     8581 aagttacatt tattgaagta atttcttact tgatactttt ctatttcatt aatctttctt
     8641 tcttctttta tattattttt attatttaaa cactccttta aatctacact cccattttta
     8701 gtaaatctat ctttaaggcc ccttgaaact ctattttgaa atcttaaatc ttttttttct
     8761 tgaaagtatt tatagatttt ttggtaacat tctttttttg aaaaattaag ttgatagtat
     8821 atttcggtcc ccaaatttac ccctaagtgt ttgtgataat tagttgtgac tttaaattct
     8881 ttttctaatt tgtaaaggta attttgcagt gtttttagtt taacgggtgt ttgactattt
     8941 cttttaaggt tttcattaaa gtaatagagt atgttttttt gtgtgtattt cttatatgtt
     9001 ttatttatgt acgctagtgt tgagataagg actactaatt tatactgtaa tttattgtag
     9061 caagttgtgg ttttactggt tttgtttata ttagataagt tttccattta tagctccttg
     9121 aataccggat tttttatcta taaagtatat atatcacaat tttgatttaa aagtaaattc
     9181 tttttttaaa aatacaaaaa ttttttataa attatataga ccatgatatt gtattaatac
     9241 taattgattt taatttaagg ttgaactaaa ataaacatag ttttgtagaa aataattttt
     9301 cattattttg ctacttgata ttggatcgta aaaatattag ggctttacaa agttctttta
     9361 aaagagaatt tagtaaagcc ctaagtcttt taataaaaaa ttttttgtaa aaaagttggc
     9421 aaaaatagtt tttgctatat atttatatat aagaaaaaat tcaatactca aggagataaa
     9481 aaatggaaaa cttaaacaat aatccacaaa aaattaatca aataaataag caaactaatt
     9541 ttattggttt tgaaaatttt atagaacttg ataatcaagt taatcaaact aaagaaaaaa
     9601 gtaaattaag taagataggt aagaaattac ccggaataag cagtcaagtc tagtgcggcc
     9661 gcatggcttg ttatgactgt ttttttgggg tacagtctat gcctcgggca tccaagcagc
     9721 aagcgcgtta cgccgtgggt cgatgtttga tgttatggag cagcaacgat gttacgcagc
     9781 agggcagtcg ccctaaaaca aagttaaaca tcatgaggag gtaacgtcat ggtaaaagat
     9841 tggattccta tttctcatga taattataaa caagtacaag gaccatttta tcatggaact
     9901 aaagctaatt tagcaattgg tgatttacta actacaggat ttatttctca ttttgaagat
     9961 ggtcgaattc ttaaacatat ttatttttca gctttaatgg aaccagcagt ttggggagct
    10021 gaacttgcta tgtcactatc tggtcttgaa ggtcgtggtt atatatatat agttgaacca
    10081 acaggaccat ttgaagatga tccaaatctt acaaataaaa aatttcctgg taatccaaca
    10141 caatcttata gaacttgtga acctttaaga attgttggtg ttgttgaaga ttgggaagga
    10201 catcctgttg aattaataag aggaatgtta gattcattag aagatttaaa acgtcgtggt
    10261 ttacatgtta ttgaagatta acctggcgcg ccaaataatg cccgtgtccc tgttgaatag
    10321 ggacacggtt ttttaacaat ttttttattc ctaggcctta gtggccggta cccggggatc
    10381 cttattactg gccgctttct agcctggata cttgtctggc tagttgcatt agagagtcag
    10441 ctagagccct agggctagct gtttgtgtat tcggagccag tgtagttttt ctttttacta
    10501 acgatacggg taataattga ttgcctttta cagcctgacc ttgagacaat tgcaaaagtc
    10561 tgtccacaga ggtttgacct aataatctaa aatcttgttt gatagtagta agtggaggta
    10621 tgtagcaact agaatcttct gtatcatcat aacccacaac tgaaatatca gccccaactc
    10681 taagtccact ttcagtaata gctcgcatag ctcctaaagc catttgatca ttagcaacta
    10741 acatagcagt agggacaatg ccttcattta acatttgcat tgtttgctga aatcctgaca
    10801 tagcagacca atctccttct ctttctgcta ttggttgtat ttgatttctt gttaaatact
    10861 tgtgccatcc tgctaatctc aatctggcac ttactgaact taagggacct gcaagtagtg
    10921 caatttgttg atggcctaat gcaacaaggt gttcaactcc taaccttgtt ccatcttcgt
    10981 gagaaaaaat tatagagttt attggggttt ggtccgacac atccaaaaac aatgctggaa
    11041 cattagtaca tgctgcttca actgcaatcg catcttggtc atcaagagga taattaatta
    11101 taagtccact aacacgttga gctaataaat tatgtactgc tgccttacaa gcctctacac
    11161 ctgatctctc aaccattgat acaacaacgc ttgcaccaag ttgatcagct ctcgatttaa
    11221 ttgcggctac aatttgacta ggcgcatgta atgcaagtga tgatgtggca actccaatta
    11281 aaagtgattg tttccccgca agttgctgag caacgcgatt tggtatataa ttcaactctg
    11341 ccatagctgc ttcaaccttc tctcttgttt ttgcagatac atgacttgct tggttcacaa
    11401 ctctcgaaac cgtttggtag ctaaccccgg catattcagc aacatcatac aaagtaacag
    11461 gtttcatgga tcattcctcc atgataaaat ttaaatttct gactttggca aatccttttg
    11521 cctgttaaaa agaatagcac aaaaattaaa aaaaaagaaa
//
