LOCUS       sequence-313118-       20471 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Human INS targeting vector for knockin of 2A separated luciferase 
            and tdTomato. Targeted cells will be puromycin resistant..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 20471)
  AUTHORS   Ma H, Jeppesen JF, Jaenisch R
  TITLE     Human T Cells Expressing a CD19 CAR-T Receptor Provide Insights into
            Mechanisms of Human CD19-Positive beta Cell Destruction.
  JOURNAL   Cell Rep Med. 2020 Sep 22;1(6):100097. doi: 
            10.1016/j.xcrm.2020.100097. eCollection 2020 Sep 22.
  PUBMED    33205073
REFERENCE   2  (bases 1 to 20471)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep 
            Med."; date: "2020-09-22"; volume: "1(6):100097. doi"; pages: " 
            10.1016/j.xcrm.2020.100097. eCollection 2020 Sep 22"
COMMENT     Sequence Label: INS-2A-luciferase-2A-tdT
FEATURES             Location/Qualifiers
     source          1..20471
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    15..136
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(52..71)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     106..125
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    181..214
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     enhancer        255..634
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        636..913
                     /label=chicken beta-actin promoter
     intron          914..1930
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1854..1876
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1938..1957
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             1991..2590
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(1991..2010)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2487..2507
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     2607..2626
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(2769..2786)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2775..2999
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    3056..3089
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        7151..7213
                     /label=Mini-TK promoter
                     /note="minimal herpes simplex virus (HSV) thymidine kinase 
                     promoter"
     5'UTR           7235..7274
                     /label=HSV TK 5'-UTR
                     /note="5' untranslated region from the herpes simplex virus
                     thymidine kinase gene"
     CDS             7292..8422
                     /codon_start=1
                     /product="herpes simplex virus thymidine kinase"
                     /label=HSV TK
                     /note="confers sensitivity to nucleoside analogs such as 
                     acyclovir or ganciclovir"
                     /translation="MASYPGHQHASAFDQAARSRGHSNRRTALRPRRQQEATEVRPEQK
                     MPTLLRVYIDGPHGMGKTTTTQLLVALGSRDDIVYVPEPMTYWRVLGASETIANIYTTQ
                     HRLDQGEISAGDAAVVMTSAQITMGMPYAVTDAVLAPHIGGEAGSSHAPPPALTLIFDR
                     HPIAALLCYPAARYLMGSMTPQAVLAFVALIPPTLPGTNIVLGALPEDRHIDRLAKRQR
                     PGERLDLAMLAAIRRVYGLLANTVRYLQGGGSWREDWGQLSGTAVPPQGAEPQSNAGPR
                     PHIGDTLFTLFRAPELLAPNGDLYNVFAWALDVLAKRLRPMHVFILDYDQSPAGCRDAL
                     LQLTSGMIQTHVTTPGSIPTICDLARTFAREMGEAN"
     primer_bind     complement(8420..8439)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    8464..8512
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     promoter        complement(9086..9104)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(9125..9141)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9125..9141)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9138..9160)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9149..9165
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9173..9179,9180..9197,9198..9203))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9218..9239
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9356..9373)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9527..10115)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9607..9626)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10286..11077,11078..11146))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10909..10928
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11147..11251)
                     /gene="bla"
                     /label=AmpR promoter
     promoter        complement(11445..11549)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      11576..12031
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11663..11682)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11873..11894
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     12157..12179
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     12171..12188
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     12172..12188
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12195..12214
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        12195..12213
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      15070..15079
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     sig_peptide     16104..16175
                     /codon_start=1
                     /product="signal sequence of human preproinsulin"
                     /label=insulin signal sequence
                     /translation="MALWMRLLPLLALLALWGPDPAAA"
     CDS             17227..17253
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             17263..17316
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             17317..18966
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTLVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(17383..17401)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     CDS             18973..19026
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             19033..20463
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
ORIGIN
        1 caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
       61 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
      121 tcatcaatgt atcttaaaat tcctgcagcc caattccgat catattcaat aacccttaat
      181 ataacttcgt ataatgtatg ctatacgaag ttattaggtc tgaagaggag tttacgtcca
      241 gccaagctag cctcgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc
      301 attagttcat agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc
      361 tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt
      421 aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca
      481 cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg
      541 taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca
      601 gtacatctac gtattagtca tcgctattac catggtcgag gtgagcccca cgttctgctt
      661 cactctcccc atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt
      721 attttgtgca gcgatggggg cggggggggg gggggggcgc gcgccaggcg gggcggggcg
      781 gggcgagggg cggggcgggg cgaggcggag aggtgcggcg gcagccaatc agagcggcgc
      841 gctccgaaag tttcctttta tggcgaggcg gcggcggcgg cggccctata aaaagcgaag
      901 cgcgcggcgg gcgggagtcg ctgcgcgctg ccttcgcccc gtgccccgct ccgccgccgc
      961 ctcgcgccgc ccgccccggc tctgactgac cgcgttactc ccacaggtga gcgggcggga
     1021 cggcccttct cctccgggct gtaattagcg cttggtttaa tgacggcttg tttcttttct
     1081 gtggctgcgt gaaagccttg aggggctccg ggagggccct ttgtgcgggg ggagcggctc
     1141 ggggggtgcg tgcgtgtgtg tgtgcgtggg gagcgccgcg tgcggctccg cgctgcccgg
     1201 cggctgtgag cgctgcgggc gcggcgcggg gctttgtgcg ctccgcagtg tgcgcgaggg
     1261 gagcgcggcc gggggcggtg ccccgcggtg cggggggggc tgcgagggga acaaaggctg
     1321 cgtgcggggt gtgtgcgtgg gggggtgagc agggggtgtg ggcgcgtcgg tcgggctgca
     1381 accccccctg cacccccctc cccgagttgc tgagcacggc ccggcttcgg gtgcggggct
     1441 ccgtacgggg cgtggcgcgg ggctcgccgt gccgggcggg gggtggcggc aggtgggggt
     1501 gccgggcggg gcggggccgc ctcgggccgg ggagggctcg ggggaggggc gcggcggccc
     1561 ccggagcgcc ggcggctgtc gaggcgcggc gagccgcagc cattgccttt tatggtaatc
     1621 gtgcgagagg gcgcagggac ttcctttgtc ccaaatctgt gcggagccga aatctgggag
     1681 gcgccgccgc accccctcta gcgggcgtgg ggcgaagcgg tgcggcgccg gcaggaagga
     1741 aatgggcggg gagggccttc gtgcgtcgcc gcgccgccgt ccccttctcc ctctccagcc
     1801 tcggggctgt ccgcgggggg acggctgcct tcggggggga cggggcaggg cggggttcgg
     1861 cttctggcgt gtgaccggcg gctctagagc ctctgctaac catgttcatg ccttcttctt
     1921 tttcctacag ctcctgggca acgtgctggt tattgtgctg tctcatcatt ttggcaaaga
     1981 ccggtccacc atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc
     2041 cagggccgta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt
     2101 cgatccggac cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt
     2161 cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac
     2221 cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggcccac gcatggccga
     2281 gttgagcggt tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg
     2341 gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa
     2401 gggtctgggc agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc
     2461 cgccttcctg gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac
     2521 cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc
     2581 cggtgcctga cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg
     2641 cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg
     2701 catcgccttc tatcgccttc ttgacgagtt cttctgaggg gatcaattct ctagagctcg
     2761 ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt
     2821 gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat
     2881 tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag
     2941 caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc
     3001 ttctgaggcg gaaagaacca gctggggctc gactagagct tgcggaaccc ttaatataac
     3061 ttcgtataat gtatgctata cgaagttatg aattcgccac tgagaagtga cttcttgttc
     3121 agtagctctg gactcttgga gtccccagag accttgttca ggaaagggaa tgagaacatt
     3181 ccagcaattt tccccccacc tagccctccc aggttctatt tttagagtta tttctgatgg
     3241 agtccctgtg gagggaggag gctgggctga gggagggggt cctgcagggc ggggggctgg
     3301 gaaggtgggg agaggctgcc gagagccacc cgctatcccc agctctgggc agccccggga
     3361 cagtcacaca ccctggcctc gcggcccaag ctggcagccg tctgcagcca cagcttatgc
     3421 cagcccaggt ccagccagac acctgaggga cccactggtg ccttggagga agcaggagag
     3481 gtcagatggc accatgagct ggggcaggtg cagggaccgt ggcagcacct ggcagggcct
     3541 cagaacccat gccttgggca ccccggccat gaggccctga ggattgcagc ccaggagaag
     3601 cagggaaccg ccagggccac aggggcagag accagggcca gggtccccct gcagcccctt
     3661 agcccacccc ctcccagtaa gcagggctgc ttggctggct tcctttgcta cagacctgct
     3721 gctcacccag aagggcccac gggccctggt gacaaggtcg ttgtggctcc aggtccttgg
     3781 gggtcctgac acagagcctc ttctgcagca cccctgagga cagggtggct ccgctgggca
     3841 cccagcctag tgggcagacg agaacctagg ggctgcctgg gcctactgtg gcctgggagg
     3901 tcagcgggtg accctagcta ccctgtggct gggccagtct gcctgccacc caggccaaac
     3961 caatctgcac ctttcctgag agctccaccc agggctgggc tggggatggc tgggcctggg
     4021 gctggcatgg gctgtggctg cagaccactg ccagcttggg cctcgaggcc aggagctcac
     4081 cctccagctg gggacctggc cactggggca gccctgttcc tgaagctctg agctcacccc
     4141 ttccccatga ccacatcagc ccccctccac ccagagatgt cacagccccc agctagcccc
     4201 gcctccagag tgggggccaa ggctgggcag gcgggtggac ggccggacac tggccccgga
     4261 agaggaggga ggcggtggct gggatcggca gcagccgtcc atgggaacac ccagccggcc
     4321 ccactcgcac gggtagagac aggggcgccc tgctggagct gaggtatgtg agctcgcgcg
     4381 gggctgggcc aaagcggggc ccggtgggcc ggctgggagg ctgcccacca gtcagccatc
     4441 ggccaagctg ttgccctggc tgaccctgat ggccaacaag gccgtaggga gtgatgggca
     4501 gaggcccctt ctgggagggg agggtcagtg ctctgtgggg gaccgtgtgt tggagtggag
     4561 ggcagcagga ggagcccttt ggtgtccagg gactcctgga gctgccccag ccttccagga
     4621 cttgcagggc agctggcact ggctggtgct gggggctgag gagtgtcttt tgaggggcca
     4681 aattttctgt gacttctgtc ctgggggacc tctgacctga ggcctcagga gagggcaagg
     4741 ccgcccaccc aaaagagatg cagccatggt tcgcggtgcc ctcggctgcc ctgggccaga
     4801 gctggggcta gctttcacct tgttgagacc caggactctg tcccccaagc ctgtcttcgc
     4861 cagcgccttg accccacccc tcatatactg tgtcctggaa aacgtggaca cgggagaccg
     4921 cagccagggc gaggtatcgc ccctccatcc ccccaggccc aatgagaagc agttggccaa
     4981 ggtgatccag gtggcagagg cagcatcaga cccagtctcc tgtcaggcac caccttgggt
     5041 gccggtcccc agatgccctg gcggggagtg tgcatgctcc cggagccccc aggtcacccc
     5101 atgtgagcca ggcccacaga gcttggctct gcaatgcctg ctgggctgct gcccatgctc
     5161 caccccttct gggaagctaa aagacagccc ttcagtgtcc agagacctgc ctggccttgg
     5221 agcctcggtt tcacatgccc accgggctgg caggggcact cagctgcctc cagccccggc
     5281 ggtcaccctg gcattgggtc catctaactg ctccccagtc acaaggcagc tgctccccaa
     5341 gtctccccaa acctgctggc ccctctagaa gcctctgtcc attcctggag gaccgagggc
     5401 agcctgcatg ccatcccgca cacagccttc tgtctgggcg tcctgccttc acacatgctg
     5461 cacagggagg aaactcttat accacattcc ttaagcagag actgaagcct ggagccaggc
     5521 acatggcacg tgctcccacc cacccaggac acactgcggt gtggctgcct ccaggctggc
     5581 cccctagatt gcgtctgctc ctggcatgga taactggcgc ctttgcctgg ccgttggggc
     5641 agtgtttgcc ttcccctgtc ggcagcaaat atttactgtc ctccgtctcc aggactctcc
     5701 aggcctgagc agaccccggg gggatgagtg tggactcagc ggtgctgagg gtagccccct
     5761 gcccttcggg tcctggtgcc cagcaggggt ccagcccagg gaagagactg aggccaggac
     5821 aggcagtgtt taagcctgag tttctgggaa aggtagccct gggcagaact tgggccgaac
     5881 gttggccagt gtctctctcc agccaggctg tgaggtagct gtttccagga tgggcacctt
     5941 tccacaccca gcaatgtggc caggagccgc cattcacggg tgcgaccagc agatggcatc
     6001 agagcctcac ttttgatgca ctccggccac cagccacggg tccaggttct ggccaccacc
     6061 cagggtctga gcagctgcat cctgcccctg ccgggcactc ccgggggctg tggggcctgt
     6121 gggggccctg ccagacactc ttgggggctg tggggggccc tgccaggcac tcccagggac
     6181 tatgggggct gtggggggcc ctgctgggca ctctgaaggg catggggctt aggaatgaga
     6241 ggagctgtct gatgatgatg gtgggggcat tgcagaggcc cccggcctgc tcaggtccag
     6301 tctcggcccc taagtcaagc ctcaggccag cctctcacca gcctgggttt ctcagagggc
     6361 cgggacaaat gttctgggtc tctaatattc caagaaagcc tctggctgga ctctgagccc
     6421 cacctgcgag cccctagaat cacagagagc tagggtgaga agaccagggg gactccgtcc
     6481 caccctcgtc gtggctgagc ccactgtggc cggtggtgga ccaggctgtg gcctttgctg
     6541 agggtcccca gggcccctgg gggctactga ggctggaggc cagcggtggc caggagggtc
     6601 cctccctcag ccactcaagc cagaaggtcg agtcctggtt tctatgtgag gagggggctt
     6661 caggggctgg gacctggggg caccgaaggc ctggagctgg ggtccaggcg gctgagggtt
     6721 agtgcgttcc cacgctcccc tccgccagcg ccgtgaggag agggaggtcc actctggaaa
     6781 gaatgtttga gggcaggggt agacagggtc tgggaacgcg gagatgacac actggggtgc
     6841 cgcctccgca ccctccgccc ttcctcccac tattgtttct ggcctcggcc gcaggaacag
     6901 caaggcagac tagtggatcc tctagagtcg agcagtgtgg ttttcaagag gaagcaaaaa
     6961 gcctctccac ccaggcctgg aatgtttcca cccaatgtcg agcagtgtgg ttttgcaaga
     7021 ggaagcaaaa agcctctcca cccaggcctg gaatgtttcc acccaatgtc gagcaaaccc
     7081 cgcccagcgt cttgtcattg gcgaattcga acacgcagat gcagtcgggg cggcgcggtc
     7141 ccaggtccac ttcgcatatt aaggtgacgc gtgtggcctc gaacaccgag cgaccctgca
     7201 gcgacccgct taacagcgtc aacagcgtgc cgcagatctt ggtggcgtga aactcccgca
     7261 cctcttcggc cagcgccttg tagaagcgcg tatggcttcg taccccggcc atcagcacgc
     7321 gtctgcgttc gaccaggctg cgcgttctcg cggccatagc aaccgacgta cggcgttgcg
     7381 ccctcgccgg cagcaagaag ccacggaagt ccgcccggag cagaaaatgc ccacgctact
     7441 gcgggtttat atagacggtc cccacgggat ggggaaaacc accaccacgc aactgctggt
     7501 ggccctgggt tcgcgcgacg atatcgtcta cgtacccgag ccgatgactt actggcgggt
     7561 gctgggggct tccgagacaa tcgcgaacat ctacaccaca caacaccgcc ttgaccaggg
     7621 tgagatatcg gccggggacg cggcggtggt aatgacaagc gcccagataa caatgggcat
     7681 gccttatgcc gtgaccgacg ccgttctggc tcctcatatc gggggggagg ctgggagctc
     7741 acatgccccg cccccggccc tcaccctcat cttcgaccgc catcccatcg ccgccctcct
     7801 gtgctacccg gccgcgcgat accttatggg cagcatgacc ccccaggccg tgctggcgtt
     7861 cgtggccctc atcccgccga ccttgcccgg cacaaacatc gtgttggggg cccttccgga
     7921 ggacagacac atcgaccgcc tggccaaacg ccagcgcccc ggcgagcggc ttgacctggc
     7981 tatgctggcc gcgattcgcc gcgtttacgg gctgcttgcc aatacggtgc ggtatctgca
     8041 gggcggcggg tcgtggcggg aggattgggg acagctttcg gggacggccg tgccgcccca
     8101 gggtgccgag ccccagagca acgcgggccc acgaccccat atcggggaca cgttatttac
     8161 cctgtttcgg gcccccgagt tgctggcccc caacggcgac ctgtacaacg tgtttgcctg
     8221 ggccttggac gtcttggcca aacgcctccg tcccatgcac gtctttatcc tggattacga
     8281 ccaatcgccc gccggctgcc gggacgccct gctgcaactt acctccggga tgatccagac
     8341 ccacgtcacc accccaggct ccataccgac gatctgcgac ctggcgcgca cgtttgcccg
     8401 ggagatgggg gaggctaact gaaacacgga aggagacaat accggaagga acccgcgcta
     8461 tgacggcaat aaaaagacag aataaaacgc acgggtgttg ggtcgtttgt tcataaacgc
     8521 ggggttcggt cccagggctg gcactctgtc gataccccac cgagacccca ttggggccaa
     8581 tacgcccgcg tttcttcctt ttccccaccc caccccccaa gttcgggtga aggcccaggg
     8641 ctcgcagcca acgtcggggc ggcaggccct gccatagcca cgggccccgt gggttaggga
     8701 cggggtcccc catggggaat ggtttatggt tcgtgggggt tattattttg ggcgttgcgt
     8761 ggggtcagtc cacgactgga ctgagcagac agacccatgg tttttggatg gcctgggcat
     8821 ggaccgcatg tactggcgcg acacgaacac cgggcgtctg tggctgccaa acacccccga
     8881 cccccaaaaa ccaccgcgcg gatttctggc gccgccggac gaactaaacc tgactacggc
     8941 atctctgccc cttcttcgct ggtacgagga gcgcttttgt tttgtattgg tcaccacggc
     9001 cgagtttccg cgggaccccg gccagatcaa gctagcttat cgataccgtc gatcgacctc
     9061 gagggggggc ccgcagcttt tgttcccttt agtgagggtt aatttcgagc ttggcgtaat
     9121 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
     9181 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
     9241 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
     9301 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
     9361 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     9421 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     9481 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     9541 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     9601 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     9661 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     9721 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     9781 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     9841 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     9901 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     9961 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
    10021 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
    10081 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
    10141 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
    10201 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
    10261 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
    10321 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
    10381 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
    10441 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
    10501 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
    10561 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
    10621 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
    10681 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
    10741 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
    10801 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
    10861 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
    10921 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
    10981 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
    11041 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
    11101 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
    11161 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
    11221 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgayg
    11281 tsyaagaaac cattattatc atgacattrr mswawawaaa tmrkcrkamc caactgatct
    11341 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
    11401 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
    11461 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
    11521 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgcg
    11581 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
    11641 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
    11701 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
    11761 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
    11821 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
    11881 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
    11941 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
    12001 aattttaaca aaatattaac gcttacaatt tccattcgcc attcaggctg cgcaactgtt
    12061 gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg
    12121 ctgcaaggcg attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga
    12181 cggccagtga attgtaatac gactcactat agggcgaatt ggagctccac cgcggtggat
    12241 gcatgtcgac aaggaaaaaa gcggccgccc ctctagcctc atcctcataa aaggtctcat
    12301 cattttccct ccagcctctt atgcactggg gaaactgagg ccaggggcta tgtgtccagc
    12361 ggacaggggt gctgaattcc acccacaggc ttagggatat ggtcaaggaa agcttcctgg
    12421 aggaggccca gtggaggttc agggagggat ggggtgcccg gcagtctcta gtggaaaagg
    12481 cgcctagcct atctccccca tgaaccccct cacccagccc tggaagaggc ctcagtgtcc
    12541 cgcctgtgac cagttggctc agaaaagccc tgggagctct gagccactgt gaaggtggaa
    12601 acgcggcccc tggcctcccc tctcctggag gctgcagact ctgcccgcca gttgacgagg
    12661 gctctgccgc tctcctcccc aggagctatg cctcacgcat ccagcgcccc ttctccgtga
    12721 agttcgaccc gtacacgctg gccatcgacg tgctggacag cccccaggcc gtgcggcgct
    12781 ccctggaggg tgtccaggat gagctggaca cccttgccca tgcgctgagt gccattggct
    12841 aggtgcacgg cgtccctgag ggcccttccc aacctcccct ggtcctgcac tgtcccggag
    12901 ctcaggccct ggtgaggggc tgggtcccgg gtgcccccca tgccctccct gctgccaggc
    12961 tcccactgcc cctgcacctg cttctcagcg caacagctgt gtgtgcccgt ggtgaggttg
    13021 tgctgcctgt ggtgaggtcc tgtcctggct cccagggtcc tgggggctgc tgcactgccc
    13081 tccgcccttc cctgacactg tctgctgccc caatcaccgt cacaataaaa gaaactgtgg
    13141 tctctacacc tgcctggccc cacatctgtg ccacagagac agaccctggg atcctcagac
    13201 tcccacaccc ccaccccagc ctcactcaga ggtttcgccc tggcctcctt cctcctctgg
    13261 gagatggctg gccgccctgg ccaggcagct ggcccctccg ggcctggttt ccccgctcac
    13321 cctgaggccc cgcccagctc tgagccccaa gcagctccag aggctcgggc accctggccg
    13381 agctgcccca tctccgtggg gtgccctccc aaggtgggga gccacgtgac agtgggaggg
    13441 cctctctcag gcctggcagg gagcaggggt cacaaactgt gctggctggg ggtggtctca
    13501 gaggtgggcc tgcaggccta accctccctg ctgacagggc tcccagccct tgagagaaac
    13561 agggatggag gaacagctgc cctgatgccc tcacccaccc ggagcaggcc ctgcgaacca
    13621 aggggaacct cagtgtggcc cccagcatgt gtgctgatgg ggagggtctg gctgagctgg
    13681 tgcccaggca gatggtctgg gcctgtctcc ccagcgaggc aggatggggg ctggatttca
    13741 gactctgtaa gatgcccctg gcttactcga ggggcctgga cattgccctc cagagagagc
    13801 acccaacacc ctccaggctt gaccggccag ggtgtcccct tcctaccttg gagagagcag
    13861 ccccagggca tcctgcaggg ggtgctggga caccagctgg ccttcaaggt ctctgcctcc
    13921 ctccagccac cccactacac gctgctggga tcctggatct cagctccccg gccgacaaca
    13981 ctggcaaact cctactcatc cacgaaggcc ctcctgggca tggtggtcct tcccagcctg
    14041 gcagtctgtt cctcacacac cttgttagtg cccagcccct gaggttgcag ctgggggtgt
    14101 ctctgaaggg ctgtgagccc ccaggaagcc ctggggaagt gcctgccttg cctccccccg
    14161 gccctgccag cgcctggctc tgccctccta cctgggctcc ccccatccag cctccctccc
    14221 tacacactcc tctcaaggag gcacccatgt cctctccagc tgccgggcct cagagcactg
    14281 tggcgtcctg gggcagccac cgcatgtcct gctgtggcat ggctcagggt ggaaagggcg
    14341 gaagggaggg gtcctgcaga tagctggtgc ccactaccaa acccgctcgg ggcaggagag
    14401 ccaaaggctg ggtgtgtgca gagcggcccc gagaggttcc gaggctgagg ccagggtggg
    14461 acatagggat gcgaggggcc ggggcacagg atactccaac ctgcctgccc ccatggtctc
    14521 atcctcctgc ttctgggacc tcctgatcct gcccctggtg ctaagaggca ggtaggggct
    14581 gcaggcagca gggctcggag cccatgcccc ctcaccatgg gtcaggctgg acctccaggt
    14641 gcctgttctg gggagctggg agggccggag gggtgtaccc caggggctca gcccagatga
    14701 cactatgggg gtgatggtgt catgggacct ggccaggaga ggggagatgg gctcccagaa
    14761 gaggagtggg ggctgagagg gtgcctgggg ggccaggacg gagctgggcc agtgcacagc
    14821 ttcccacacc tgcccacccc cagagtcctg ccgccacccc cagatcacac ggaagatgag
    14881 gtccgagtgg cctgctgagg acttgctgct tgtccccagg tccccaggtc atgccctcct
    14941 tctgccaccc tggggagctg agggcctcag ctggggctgc tgtcctaagg cagggtggga
    15001 actaggcagc cagcagggag gggacccctc cctcactccc actctcccac ccccaccacc
    15061 ttggcccatc catggcggca tcttgggcca tccgggactg gggacagggg tcctggggac
    15121 aggggtgtgg ggacaggggt cctggggaca ggggtctggg gacaggggtc ctggggacag
    15181 gggtgtgggg acaggggtgt ggggacaggg gtgtggggac aggggtcctg gggacagggg
    15241 tctggggaca ggggtctgag gacaggggtg tggggacagg ggtgtgggga caggggtgtg
    15301 gggacagggg tstggggaca ggggtctggg gacaggggtc cgggggacag gggtgtgggg
    15361 acaggggtgt ggggacaggg gtgtggggac aggggtctgg ggacaggggt gtggggacag
    15421 gggtcctggg gacaggggtg tggggatagg ggtgtgggga caggggtgtg gggacagggg
    15481 tgtggggaca ggggtctggg gacagcagcg caaagagccc cgccctgcag cctccagctc
    15541 tcctggtcta atgtggaaag tggcccaggt gagggctttg ctctcctgga gacatttgcc
    15601 cccagctgtg agcagggaca ggtctggcca ccgggcccct ggttaagact ctaatgaccc
    15661 gctggtcctg aggaagaggt gctgacgacc aaggagatct tcccacagac ccagcaccag
    15721 ggaaatggtc cggaaattgc agcctcagcc cccagccatc tgccgacccc cccaccccag
    15781 gccctaatgg gccaggcggc aggggttgag aggtagggga gatgggctct gagactataa
    15841 agccagcggg ggcccagcag ccctcagccc tccaggacag gctgcatcag aagaggccat
    15901 caagcaggtc tgttccaagg gcctttgcgt caggtgggct caggattcca gggtggctgg
    15961 accccaggcc ccagctctgc agcagggagg acgtggctgg gctcgtgaag catgtggggg
    16021 tgagcccagg ggccccaagg cagggcacct ggccttcagc ctgcctcagc cctgcctgtc
    16081 tcccagatca ctgtccttct gccatggccc tgtggatgcg cctcctgccc ctgctggcgc
    16141 tgctggccct ctggggacct gacccagccg cagcctttgt gaaccaacac ctgtgcggct
    16201 cacacctggt ggaagctctc tacctagtgt gcggggaacg aggcttcttc tacacaccca
    16261 agacccgccg ggaggcagag gacctgcagg gtgagccaac tgcccattgc tgcccctggc
    16321 cgcccccagc caccccctgc tcctggcgct cccacccagc atgggcagaa gggggcagga
    16381 ggctgccacc cagcaggggg tcaggtgcac ttttttaaaa agaagttctc ttggtcacgt
    16441 cctaaaagtg accagctccc tgtggcccag tcagaatctc agcctgagga cggtgttggc
    16501 ttcggcagcc ccgagataca tcagagggtg ggcacgctcc tccctccact cgcccctcaa
    16561 acaaatgccc cgcagcccat ttctccaccc tcatttgatg accgcagatt caagtgtttt
    16621 gttaagtaaa gtcctgggtg acctggggtc acagggtgcc ccacgctgcc tgcctctggg
    16681 cgaacacccc atcacgcccg gaggagggcg tggctgcctg cctgagtggg ccagacccct
    16741 gtcgccaggc ctcacggcag ctccatagtc aggagatggg gaagatgctg gggacaggcc
    16801 ctggggagaa gtactgggat cacctgttca ggctcccact gtgacgctgc cccggggcgg
    16861 gggaaggagg tgggacatgt gggcgttggg gcctgtaggt ccacacccag tgtgggtgac
    16921 cctccctcta acctgggtcc agcccggctg gagatgggtg ggagtgcgac ctagggctgg
    16981 cgggcaggcg ggcactgtgt ctccctgact gtgtcctcct gtgtccctct gcctcgccgc
    17041 tgttccggaa cctgctctgc gcggcacgtc ctggcagtgg ggcaggtgga gctgggcggg
    17101 ggccctggtg caggcagcct gcagcccttg gccctggagg ggtccctgca gaagcgtggc
    17161 attgtggaac aatgctgtac cagcatctgc tccctctacc agctggagaa ctactgcaac
    17221 ggtacctacc catacgatgt tccagattac gctggcagtg gagagggcag aggaagtctg
    17281 ctaacatgcg gtgacgtcga ggagaatcct ggcccaatgg aagacgccaa aaacataaag
    17341 aaaggcccgg cgccattcta tccgctggaa gatggaaccg ctggagagca actgcataag
    17401 gctatgaaga gatacgccct ggttcctgga acaattgctt ttacagatgc acatatcgag
    17461 gtggacatca cttacgctga gtacttcgaa atgtccgttc ggttggcaga agctatgaaa
    17521 cgatatgggc tgaatacaaa tcacagaatc gtcgtatgca gtgaaaactc tcttcaattc
    17581 tttatgccgg tgttgggcgc gttatttatc ggagttgcag ttgcgcccgc gaacgacatt
    17641 tataatgaac gtgaattgct caacagtatg ggcatttcgc agcctaccgt ggtgttcgtt
    17701 tccaaaaagg ggttgcaaaa aattttgaac gtgcaaaaaa agctcccaat catccaaaaa
    17761 attattatca tggattctaa aacggattac cagggatttc agtcgatgta cacgctcgtc
    17821 acatctcatc tacctcccgg ttttaatgaa tacgattttg tgccagagtc cttcgatagg
    17881 gacaagacaa ttgcactgat catgaactcc tctggatcta ctggtctgcc taaaggtgtc
    17941 gctctgcctc atagaactgc ctgcgtgaga ttctcgcatg ccagagatcc tatttttggc
    18001 aatcaaatca ttccggatac tgcgatttta agtgttgttc cattccatca cggttttgga
    18061 atgtttacta cactcggata tttgatatgt ggatttcgag tcgtcttaat gtatagattt
    18121 gaagaagagc tgtttctgag gagccttcag gattacaaga ttcaaagtgc gctgctggtg
    18181 ccaaccctat tctccttctt cgccaaaagc actctgattg acaaatacga tttatctaat
    18241 ttacacgaaa ttgcttctgg tggcgctccc ctctctaagg aagtcgggga agcggttgcc
    18301 aagaggttcc atctgccagg tatcaggcaa ggatatgggc tcactgagac tacatcagct
    18361 attctgatta cacccgaggg ggatgataaa ccgggcgcgg tcggtaaagt tgttccattt
    18421 tttgaagcga aggttgtgga tctggatacc gggaaaacgc tgggcgttaa tcaaagaggc
    18481 gaactgtgtg tgagaggtcc tatgattatg tccggttatg taaacaatcc ggaagcgacc
    18541 aacgccttga ttgacaagga tggatggcta cattctggag acatagctta ctgggacgaa
    18601 gacgaacact tcttcatcgt tgaccgcctg aagtctctga ttaagtacaa aggctatcag
    18661 gtggctcccg ctgaattgga atccatcttg ctccaacacc ccaacatctt cgacgcaggt
    18721 gtcgcaggtc ttcccgacga tgacgccggt gaacttcccg ccgccgttgt tgttttggag
    18781 cacggaaaga cgatgacgga aaaagagatc gtggattacg tcgccagtca agtaacaacc
    18841 gcgaaaaagt tgcgcggagg agttgtgttt gtggacgaag taccgaaagg tcttaccgga
    18901 aaactcgacg caagaaaaat cagagagatc ctcataaagg ccaagaaggg cggaaagatc
    18961 gccgtggtcg acgagggcag aggcagcctg ctgacatgtg gcgacgtgga agagaaccct
    19021 ggccccatgc atatggtgag caagggcgag gaggtcatca aagagttcat gcgcttcaag
    19081 gtgcgcatgg agggctccat gaacggccac gagttcgaga tcgagggcga gggcgagggc
    19141 cgcccctacg agggcaccca gaccgccaag ctgaaggtga ccaagggcgg ccccctgccc
    19201 ttcgcctggg acatcctgtc cccccagttc atgtacggct ccaaggcgta cgtgaagcac
    19261 cccgccgaca tccccgatta caagaagctg tccttccccg agggcttcaa gtgggagcgc
    19321 gtgatgaact tcgaggacgg cggtctggtg accgtgaccc aggactcctc cctgcaggac
    19381 ggcacgctga tctacaaggt gaagatgcgc ggcaccaact tcccccccga cggccccgta
    19441 atgcagaaga agaccatggg ctgggaggcc tccaccgagc gcctgtaccc ccgcgacggc
    19501 gtgctgaagg gcgagatcca ccaggccctg aagctgaagg acggcggcca ctacctggtg
    19561 gagttcaaga ccatctacat ggccaagaag cccgtgcaac tgcccggcta ctactacgtg
    19621 gacaccaagc tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgag
    19681 cgctccgagg gccgccacca cctgttcctg gggcatggca ccggcagcac cggcagcggc
    19741 agctccggca ccgcctcctc cgaggacaac aacatggccg tcatcaaaga gttcatgcgc
    19801 ttcaaggtgc gcatggaggg ctccatgaac ggccacgagt tcgagatcga gggcgagggc
    19861 gagggccgcc cctacgaggg cacccagacc gccaagctga aggtgaccaa gggcggcccc
    19921 ctgcccttcg cctgggacat cctgtccccc cagttcatgt acggctccaa ggcgtacgtg
    19981 aagcaccccg ccgacatccc cgattacaag aagctgtcct tccccgaggg cttcaagtgg
    20041 gagcgcgtga tgaacttcga ggacggcggt ctggtgaccg tgacccagga ctcctccctg
    20101 caggacggca cgctgatcta caaggtgaag atgcgcggca ccaacttccc ccccgacggc
    20161 cccgtaatgc agaagaagac catgggctgg gaggcctcca ccgagcgcct gtacccccgc
    20221 gacggcgtgc tgaagggcga gatccaccag gccctgaagc tgaaggacgg cggccactac
    20281 ctggtggagt tcaagaccat ctacatggcc aagaagcccg tgcaactgcc cggctactac
    20341 tacgtggaca ccaagctgga catcacctcc cacaacgagg actacaccat cgtggaacag
    20401 tacgagcgct ccgagggccg ccaccacctg ttcctgtacg gcatggacga gctgtacaag
    20461 taattaatta a
//
