LOCUS       sequence-31312-e         865 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 865)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 865)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: ZNF289
FEATURES             Location/Qualifiers
     source          1..865
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             45..62
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             72..89
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             494..511
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(564..582)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      578..625
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(749..768)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 atattttgtt tactttaaga aggagatata ccatgggcag cagccatcat catcatcatc
       61 acagcagcgg cctggttccg cgtggtagtg cggagccgaa caagaccgaa atccagactc
      121 tttttaagag gcttcgcgca gttccaacca acaaggcctg tttcgactgc ggcgccaaga
      181 atccgagttg ggccagcatc acgtacggtg ttttcttgtg cattgactgt tccggggtgc
      241 accgctccct gggcgtccat ctgagcttca tcaggtccac agagttggat tccaactgga
      301 actggttcca gctgaggtgt atgcaggtcg gcgggaatgc caatgcgacg gctttttttc
      361 gccaacatgg atgcacagcc aatgatgcca acaccaaata taatagccga gctgcccaga
      421 tgtaccggga gaagatccgg cagctgggga gtgcggccct ggcttgatga cgaagcttgc
      481 ggccgcactc gagcaccacc accaccacca ctgagatccg gctgctaaca aagcccgaaa
      541 ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc
      601 taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat tggcgaatgg
      661 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
      721 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
      781 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
      841 agtgctttac ggcacctcga cccca
//
