LOCUS       sequence-313121-        4049 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 Golden Gate vector, containing the ZmUbi promoter and 5' UTR
            with Level 0.5-compatible overhangs (Wheat construct).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4049)
  AUTHORS   Harrington SA, Backhaus AE, Fox S, Rogers C, Borrill P, Uauy C, 
            Richardson A
  TITLE     A heat-shock inducible system for flexible gene expression in 
            cereals.
  JOURNAL   Plant Methods. 2020 Oct 14;16:137. doi: 10.1186/s13007-020-00677-3. 
            eCollection 2020.
  PUBMED    33072173
REFERENCE   2  (bases 1 to 4049)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Methods."; date: "2020-10-14"; volume: "16:137. doi"; pages: " 
            10.1186/s13007-020-00677-3. eCollection 2020"
COMMENT     Sequence Label: pZmUbi-5'UTR
FEATURES             Location/Qualifiers
     source          1..4049
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      10..67
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(91..108)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(262..850)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(342..361)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1033..1127)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1148..1807)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1722..1741
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     1969..1987
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(1991..2034)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
ORIGIN
        1 accctgcagt ccggcaaaaa agggcaaggt gtcaccaccc tgcccttttt ctttaaaacc
       61 gaaaagatta cttcgcgtta tgcaggcttc ctcgctcact gactcgctgc gctcggtcgt
      121 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
      181 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
      241 aaaggccgcg ttgctggcgt ttttccacag gctccgcccc cctgacgagc atcacaaaaa
      301 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
      361 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
      421 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
      481 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
      541 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
      601 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
      661 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
      721 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
      781 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
      841 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
      901 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
      961 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     1021 ctcgaggctt ggattctcac caataaaaaa cgcccggcgg caaccgagcg ttctgaacaa
     1081 atccagatgg agttctgagg tcattactgg atctatcaac aggagtccaa gcgagctcga
     1141 tatcaaatta cgccccgccc tgccactcat cgcagtactg ttgtaattca ttaagcattc
     1201 tgccgacatg gaagccatca caaacggcat gatgaacctg aatcgccagc ggcatcagca
     1261 ccttgtcgcc ttgcgtataa tatttgccca tggtgaaaac gggggcgaag aagttgtcca
     1321 tattggccac gtttaaatca aaactggtga aactcaccca gggattggct gagacgaaaa
     1381 acatattctc aataaaccct ttagggaaat aggccaggtt ttcaccgtaa cacgccacat
     1441 cttgcgaata tatgtgtaga aactgccgga aatcgtcgtg gtattcactc cagagcgatg
     1501 aaaacgtttc agtttgctca tggaaaacgg tgtaacaagg gtgaacacta tcccatatca
     1561 ccagctcacc gtctttcatt gccatacgaa attccggatg agcattcatc aggcgggcaa
     1621 gaatgtgaat aaaggccgga taaaacttgt gcttattttt ctttacggtc tttaaaaagg
     1681 ccgtaatatc cagctgaacg gtctggttat aggtacattg agcaactgac tgaaatgcct
     1741 caaaatgttc tttacgatgc cattgggata tatcaacggt ggtatatcca gtgatttttt
     1801 tctccatttt agcttcctta gctcctgaaa atctcgataa ctcaaaaaat acgcccggta
     1861 gtgatcttat ttcattatgg tgaaagttgg aacctcttac gtgcccgatc aactcgagtg
     1921 ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc
     1981 acgaggcaga atttcagata aaaaaaatcc ttagctttcg ctaaggatga tttctggaat
     2041 tcggtctcag gaggtgcagc gtgacccggt cgtgcccctc tctagagata atgagcattg
     2101 catgtctaag ttataaaaaa ttaccacata ttttttttgt cacacttgtt tgaagtgcag
     2161 tttatctatc tttatacata tatttaaact ttactctacg aataatataa tctatagtac
     2221 tacaataata tcagtgtttt agagaatcat ataaatgaac agttagacat ggtctaaagg
     2281 acaattgagt attttgacaa caggactcta cagttttatc tttttagtgt gcatgtgttc
     2341 tccttttttt ttgcaaatag cttcacctat ataatacttc atccatttta ttagtacatc
     2401 catttagggt ttagggttaa tggtttttat agactaattt ttttagtaca tctattttat
     2461 tctattttag cctctaaatt aagaaaacta aaactctatt ttagtttttt tatttaataa
     2521 tttagatata aaatagaata aaataaagtg actaaaaatt aaacaaatac cctttaagaa
     2581 attaaaaaaa ctaaggaaac atttttcttg tttcgagtag ataatgccag cctgttaaac
     2641 gccgtcgacg agtctaacgg acaccaacca gcgaaccagc agcgtcgcgt cgggccaagc
     2701 gaagcagacg gcacggcatc tctgtcgctg cctctggacc cctctcgaga gttccgctcc
     2761 accgttggac ttgctccgct gtcggcatcc agaaattgcg tggcggagcg gcagacgtga
     2821 gccggcacgg caggcggcct cctcctcctc tcacggcacg gcagctacgg gggattcctt
     2881 tcccaccgct ccttcgcttt cccttcctcg cccgccgtaa taaatagaca ccccctccac
     2941 accctctttc cccaacctcg tgttgttcgg agcgcacaca cacacaacca gatctccccc
     3001 aaatccaccc gtcggcacct ccgcttcaag gtacgccgct cgtcctcccc ccccccccct
     3061 ctctaccttc tctagatcgg cgttccggtc catggttagg gcccggtagt tctacttctg
     3121 ttcatgtttg tgttagatcc gtgtttgtgt tagatccgtg ctgctagcgt tcgtacacgg
     3181 atgcgacctg tacgtcagac acgttctgat tgctaacttg ccagtgtttc tctttgggga
     3241 atcctgggat ggctctagcc gttccgcaga cgggatcgat ttcatgattt tttttgtttc
     3301 gttgcatagg gtttggtttg cccttttcct ttatttcaat atatgccgtg cacttgtttg
     3361 tcgggtcatc ttttcatgct tttttttgtc ttggttgtga tgatgtggtc tggttgggcg
     3421 gtcgttctag atcggagtag aattctgttt caaactacct ggtggattta ttaattttgg
     3481 atctgtatgt gtgtgccata catattcata gttacgaatt gaagatgatg gatggaaata
     3541 tcgatctagg ataggtatac atgttgatgc gggttttact gatgcatata cagagatgct
     3601 ttttgttcgc ttggttgtga tgatgtggtg tggttgggcg gtcgttcatt cgttctagat
     3661 cggagtagaa tactgtttca aactacctgg tgtatttatt aattttggaa ctgtatgtgt
     3721 gtgtcataca tcttcatagt tacgagttta agatggatgg aaatatcgat ctaggatagg
     3781 tatacatgtt gatgtgggtt ttactgatgc atatacatga tggcatatgc agcatctatt
     3841 catatgctct aaccttgagt acctatctat tataataaac aagtatgttt tataattatt
     3901 ttgatcttga tatacttgga tgatggcata tgcagcagct atatgtggat ttttttagcc
     3961 ctgccttcat acgctattta tttgcttggt actgtttctt ttgtcgatgc tcaccctgtt
     4021 gtttggtgtt acttctgcag gtactcgag
//
