LOCUS       sequence-313127-        4357 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  p5E with promoter sequence 1.7kb upstream of zebrafish fabp6 gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4357)
  AUTHORS   Wen J, Mercado GP, Volland A, Doden HL, Lickwar CR, Crooks T, 
            Kakiyama G, Kelly C, Cocchiaro JL, Ridlon JM, Rawls JF
  TITLE     Fxr signaling and microbial metabolism of bile salts in the 
            zebrafish intestine.
  JOURNAL   Sci Adv. 2021 Jul 23;7(30). pii: 7/30/eabg1371. doi: 
            10.1126/sciadv.abg1371. Print 2021 Jul.
  PUBMED    34301599
REFERENCE   2  (bases 1 to 4357)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2021-07-23"; volume: "7(30). pii: 7/30/eabg1371. doi"; pages:
            " 10.1126/sciadv.abg1371. Print 2021 Jul"
COMMENT     Sequence Label: p5E-1.7kbfabp6
FEATURES             Location/Qualifiers
     source          1..4357
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1518..1641
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1713..1732)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1714..1732)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1737..1753)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1737..1753)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1855..1874)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1866..2675
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1933..1952)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2822..3410
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3311..3330
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3564..3581
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3740..3767)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3859..3945)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3994..4016
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4008..4025
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4009..4025
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    4065..4160
                     /gene="mutant version of attL"
                     /label=attL4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 gttttacaca caaaaacatt gaaaccagta gcattgacgt ccgttttccc atatactcaa
       61 aaatattttc acagcttgtt caaactactt ctttaatatg agctaaatca acacaactgc
      121 tgaggtgttt tttgacagct taatagtttt attttcaatc cattgtaact agtaaaaaca
      181 tttacagttg aaaccagaag tttacataca ctgtataata aggcacataa caattaaaaa
      241 aaaaaagtca aatgttaacg tgaccaagct ttttcttttt taggtagggt aggattatca
      301 catttgtttc tgttatactt aatagcagaa taatgagaga taattttttg agaaattatt
      361 gtaacttttc ttgaaagtca agtttacata caataagatt attatgcctt tgaaaaagct
      421 cagatgatgg tgtcaaggtt ttggaagttt ctgattggct aattgacaac atttgagtta
      481 attggaagct gttgaatagt attcaagaaa aacctcaaac acactgcttc cttgtgtgac
      541 aacatgggac aataaacaag ccagaatcaa caaaaaagcc agattacagt ttgctaaatt
      601 acactgggta aaagacaaat ttttggagac atgtcctgtg gtctgataga actaagattg
      661 aactgtttcg ccataatgac cagtgctaca tttggaggat gaaagggaaa gtttacaagc
      721 ctcggaacat catctctact gtgatgtatg gggctggcag catcatgttg ttgggctgtt
      781 ttgctgcagg agggactggt ccacttcaca gcatagatgg catcatgatg aaagcatttt
      841 gtggaaatac tgaggcaaca tctcaagaca tcagcaagga aattaaaact tggccacaaa
      901 tgggtcttcc aaacatacca tgaccctaag catgctgcca aattagttaa aatgtacttt
      961 aaggacaaca gagtgaatgt tttggagtgg ccatcacaaa gccctgagct caatcctata
     1021 gaaaatttgt gggtagattt gaaaaagctt gtgcgagcaa aacagccaac aaatttgact
     1081 tagttacacc aattccgtca ggaggaatga gccaaaattc cttcaaacta ttgtgagaag
     1141 cttgtggaag gatacccaaa acatttgacc aaagttatac agtttaaaag caaagctaaa
     1201 aaattcttat ggtaattttt ttgtattgta ttataattgt attgcacgat tattatgaag
     1261 tgattttgcc catgtatggc atatcatcaa ccatgtattt tgcccatgta aggcatatca
     1321 tcactaccca ggtgagcagc tgtgcggtca tgcactttat gacttactct attttgtggg
     1381 gccatcataa atgtttatgg cttgattatg atgctggtgt accactaagg ccatgagaaa
     1441 gagtcagagt tgaggaacag ttgggtatat aaacaccgca cacttcaccc acagtacctc
     1501 agctctcaag gcgcgcccaa gtttgtacaa aaaagttgaa cgagaaacgt aaaatgatat
     1561 aaatatcaat atattaaatt agattttgca taaaaaacag actacataat actgtaaaac
     1621 acaacatatg cagtcactat gaaccaacta cttagatggt attagtgacc tgtagaattc
     1681 gagctctaga gctgcagggc ggccgcgata tcccctatag tgagtcgtat tacatggtca
     1741 tagctgtttc ctggcagctc tggcccgtgt ctcaaaatct ctgatgttac attgcacaag
     1801 ataaaaatat atcatcatga acaataaaac tgtctgctta cataaacagt aatacaaggg
     1861 gtgttatgag ccatattcaa cgggaaacgt cgaggccgcg attaaattcc aacatggatg
     1921 ctgatttata tgggtataaa tgggctcgcg ataatgtcgg gcaatcaggt gcgacaatct
     1981 atcgcttgta tgggaagccc gatgcgccag agttgtttct gaaacatggc aaaggtagcg
     2041 ttgccaatga tgttacagat gagatggtca gactaaactg gctgacggaa tttatgcctc
     2101 ttccgaccat caagcatttt atccgtactc ctgatgatgc atggttactc accactgcga
     2161 tccccggaaa aacagcattc caggtattag aagaatatcc tgattcaggt gaaaatattg
     2221 ttgatgcgct ggcagtgttc ctgcgccggt tgcattcgat tcctgtttgt aattgtcctt
     2281 ttaacagcga tcgcgtattt cgtctcgctc aggcgcaatc acgaatgaat aacggtttgg
     2341 ttgatgcgag tgattttgat gacgagcgta atggctggcc tgttgaacaa gtctggaaag
     2401 aaatgcataa acttttgcca ttctcaccgg attcagtcgt cactcatggt gatttctcac
     2461 ttgataacct tatttttgac gaggggaaat taataggttg tattgatgtt ggacgagtcg
     2521 gaatcgcaga ccgataccag gatcttgcca tcctatggaa ctgcctcggt gagttttctc
     2581 cttcattaca gaaacggctt tttcaaaaat atggtattga taatcctgat atgaataaat
     2641 tgcagtttca tttgatgctc gatgagtttt tctaatcaga attggttaat tggttgtaac
     2701 actggcagag cattacgctg acttgacggg acggcgcaag ctcatgacca aaatccctta
     2761 acgtgagtta cgcgtcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     2821 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     2881 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     2941 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
     3001 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     3061 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     3121 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     3181 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     3241 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     3301 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     3361 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     3421 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     3481 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     3541 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa
     3601 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt
     3661 ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tacgcgtacc gctagccagg
     3721 aagagtttgt agaaacgcaa aaaggccatc cgtcaggatg gccttctgct tagtttgatg
     3781 cctggcagtt tatggcgggc gtcctgcccg ccaccctccg ggccgttgct tcacaacgtt
     3841 caaatccgct cccggcggat ttgtcctact caggagagcg ttcaccgaca aacaacagat
     3901 aaaacgaaag gcccagtctt ccgactgagc ctttcgtttt atttgatgcc tggcagttcc
     3961 ctactctcgc gttaacgcta gcatggatgt tttcccagtc acgacgttgt aaaacgacgg
     4021 ccagtcttaa gctcgggccc gagttaacgc taccatggag ctccaaataa tgattttatt
     4081 ttgactgata gtgacctgtt cgttgcaaca aattgataag caatgctttt ttataatgcc
     4141 aactttgtat agaaaagttg ggccggccga tgatcccaac cctgtaatga gttcctggtt
     4201 tttaaatgta ttttatttgt tggtaaccat ttatagcatt gactcaagca actattcttt
     4261 ttaaaagaaa tcttttttta agctcaccca aactgaaaat tgtgtcataa gtttctctcc
     4321 tttgctcatc acaaacctgt ttgtgtttct attttct
//
