LOCUS       sequence-313129-        8563 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  LR construct using backbone with Cry:mCherry insert to identify fish
            with transgene, and with p5E-1.7kbfabp6, pME-eGFP, and p3E-pA 
            inserts.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8563)
  AUTHORS   Wen J, Mercado GP, Volland A, Doden HL, Lickwar CR, Crooks T, 
            Kakiyama G, Kelly C, Cocchiaro JL, Ridlon JM, Rawls JF
  TITLE     Fxr signaling and microbial metabolism of bile salts in the 
            zebrafish intestine.
  JOURNAL   Sci Adv. 2021 Jul 23;7(30). pii: 7/30/eabg1371. doi: 
            10.1126/sciadv.abg1371. Print 2021 Jul.
  PUBMED    34301599
REFERENCE   2  (bases 1 to 8563)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2021-07-23"; volume: "7(30). pii: 7/30/eabg1371. doi"; pages:
            " 10.1126/sciadv.abg1371. Print 2021 Jul"
COMMENT     Sequence Label: pDest-1.7kbfabp6-eGFP-pA-CrymCherry
FEATURES             Location/Qualifiers
     source          1..8563
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(51..761)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEDDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGHHSTGGMDELYK"
     primer_bind     complement(131..154)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(329..348)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     598..616
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     regulatory      758..767
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     1409..1425
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1445..1465
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    3179..3203
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(3209..3211,3212..3214,3215..3925)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3209..3211,3212..3214,3215..3925)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3254..3275)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3515..3534)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3862..3883
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(3987..4011)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    4013..4147
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(4210..4230)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(4238..4254)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    4270..4404
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    join(4843..5030,5031..5042)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(5076..5092)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5076..5092)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(5129..5149)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(5129..5147)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5168..5184)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5181..5203)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5192..5208
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5216..5222,5223..5240,5241..5246))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5261..5282
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5399..5416)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5570..6158)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5650..5669)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6329..7120,7121..7189))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6952..6971
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7190..7294)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(7457..7478)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    join(7827..7838,7839..8001)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     polyA_signal    8370..8491
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 aacctctaca aatgtggtat ggctgattat gatctagagt cgcggccgct ttacttgtac
       61 agctcgtcca tgccgccggt ggagtggtgg ccctcggcgc gctcgtactg ttccacgatg
      121 gtgtagtcct cgttgtggga ggtgatgtcc agcttgatgt tgacgttgta ggcgccgggc
      181 agctgcacgg gcttcttggc cttgtaggtg gtcttgacct cggcgtcgta gtggccgccg
      241 tccttcagct tcagcctctg cttgatctcg cccttcaggg cgccgtcctc ggggtacatc
      301 cgctcggagg aggcctccca gcccatggtc ttcttctgca ttacggggcc gtcggagggg
      361 aagttggtgc cgcgcagctt caccttgtag atgaactcgc cgtcctgcag ggaggagtcc
      421 tgggtcacgg tcaccacgcc gccgtcctcg aagttcatca cgcgctccca cttgaagccc
      481 tcggggaagg acagcttcaa gtagtcgggg atgtcggcgg ggtgcttcac gtaggccttg
      541 gagccgtaca tgaactgagg ggacaggatg tcccaggcga agggcagggg gccgcccttg
      601 gtcaccttca gcttggcggt ctgggtgccc tcgtaggggc ggccctcgcc ctcgccctcg
      661 atctcgaact cgtggccgtt cacggagccc tccatgtgca ccttgaagcg catgaactcc
      721 ttgatgatgg ccatgttgtc atcctcgccc ttgctcacca tggtggcgac cggtaatgtc
      781 agacctggta actccttact gtaacttatt gcacctgatg aacaaaagat gtgaactgaa
      841 cgcaagtagg aggctaatag aggctagtac agtacaaaga tccccaaatg atgtgtgccg
      901 gccttagtgg aggcagaccc ctggttatat acctgtgctg cagaaagagg ggcttacaaa
      961 tggatctctc tggaatggca ttatctcatg gtgggaacag tgttgtcagc agaatcagcg
     1021 gggtggactc tgacctgaga atgacagtct agcactgaat ggctcagaca atactcttgt
     1081 ggccaactat caccggaaaa taagtgcctg ccaaaacaaa ccagaccaga tgaagctaca
     1141 tggctgtaga acgtcagtat ttaatgtgtt gtggccactt ggttcaccag atttaagagc
     1201 taattttttt aaatatcaca gataatcaat cgcttttttt tttcttgact gaaaagctta
     1261 gactggaaaa ttctttgaaa aagtttgcac tacagaaaat aaatacataa catcctgcac
     1321 tgaatgcaca ctattaatag aaaagagagc attattgtag gaaatgtatt gtatatgtac
     1381 attctgtgca tttcacaatt ttctcgagca ggaaacagct atgaccatga ttacgccaag
     1441 ctatcaactt tgtatagaaa agttgggccg gccgatgatc ccaaccctgt aatgagttcc
     1501 tggtttttaa atgtatttta tttgttggta accatttata gcattgactc aagcaactat
     1561 tctttttaaa agaaatcttt ttttaagctc acccaaactg aaaattgtgt cataagtttc
     1621 tctcctttgc tcatcacaaa cctgtttgtg tttctatttt ctgttttaca cacaaaaaca
     1681 ttgaaaccag tagcattgac gtccgttttc ccatatactc aaaaatattt tcacagcttg
     1741 ttcaaactac ttctttaata tgagctaaat caacacaact gctgaggtgt tttttgacag
     1801 cttaatagtt ttattttcaa tccattgtaa ctagtaaaaa catttacagt tgaaaccaga
     1861 agtttacata cactgtataa taaggcacat aacaattaaa aaaaaaaagt caaatgttaa
     1921 cgtgaccaag ctttttcttt tttaggtagg gtaggattat cacatttgtt tctgttatac
     1981 ttaatagcag aataatgaga gataattttt tgagaaatta ttgtaacttt tcttgaaagt
     2041 caagtttaca tacaataaga ttattatgcc tttgaaaaag ctcagatgat ggtgtcaagg
     2101 ttttggaagt ttctgattgg ctaattgaca acatttgagt taattggaag ctgttgaata
     2161 gtattcaaga aaaacctcaa acacactgct tccttgtgtg acaacatggg acaataaaca
     2221 agccagaatc aacaaaaaag ccagattaca gtttgctaaa ttacactggg taaaagacaa
     2281 atttttggag acatgtcctg tggtctgata gaactaagat tgaactgttt cgccataatg
     2341 accagtgcta catttggagg atgaaaggga aagtttacaa gcctcggaac atcatctcta
     2401 ctgtgatgta tggggctggc agcatcatgt tgttgggctg ttttgctgca ggagggactg
     2461 gtccacttca cagcatagat ggcatcatga tgaaagcatt ttgtggaaat actgaggcaa
     2521 catctcaaga catcagcaag gaaattaaaa cttggccaca aatgggtctt ccaaacatac
     2581 catgacccta agcatgctgc caaattagtt aaaatgtact ttaaggacaa cagagtgaat
     2641 gttttggagt ggccatcaca aagccctgag ctcaatccta tagaaaattt gtgggtagat
     2701 ttgaaaaagc ttgtgcgagc aaaacagcca acaaatttga cttagttaca ccaattccgt
     2761 caggaggaat gagccaaaat tccttcaaac tattgtgaga agcttgtgga aggataccca
     2821 aaacatttga ccaaagttat acagtttaaa agcaaagcta aaaaattctt atggtaattt
     2881 ttttgtattg tattataatt gtattgcacg attattatga agtgattttg cccatgtatg
     2941 gcatatcatc aaccatgtat tttgcccatg taaggcatat catcactacc caggtgagca
     3001 gctgtgcggt catgcacttt atgacttact ctattttgtg gggccatcat aaatgtttat
     3061 ggcttgatta tgatgctggt gtaccactaa ggccatgaga aagagtcaga gttgaggaac
     3121 agttgggtat ataaacaccg cacacttcac ccacagtacc tcagctctca aggcgcgccc
     3181 aagtttgtac aaaaaagcag gctggaccat ggtgagcaag ggcgaggagc tgttcaccgg
     3241 ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc
     3301 cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac
     3361 cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg
     3421 cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga
     3481 aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc
     3541 cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt
     3601 caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt
     3661 ctatatcatg gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa
     3721 catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga
     3781 cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccgccc tgagcaaaga
     3841 ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac
     3901 tctcggcatg gacgagctgt acaagggcgg tggaagatct gggaattcaa ggcctctcga
     3961 gcctctagat tctgcagccc tatagcaccc agctttcttg tacaaagtgg gggatccaga
     4021 catgataaga tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg
     4081 ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa
     4141 acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg aggtgtggga
     4201 ggttttttcc aactttatta tacatagttg ataattcact ggccgtcgtt ttacggtacc
     4261 atcgatgatg atccagacat gataagatac attgatgagt ttggacaaac cacaactaga
     4321 atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc
     4381 attataagct gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt
     4441 cagggggagg tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg tggtatggct
     4501 gattatgatc ctctagatca gatctgcgaa gatacggcca cgggtgctct tgatcctgtg
     4561 gctgattttg gactgtgctg ctcgcagctg ctgatgaatc acatacttcc tccattttct
     4621 tccactgatt gactgttata atttccctaa tttccaggtc aaggtgctgt gcattgtggt
     4681 aatagatgtg acatgacgtc acttccaaag gaccaatgaa catgtctgac caatttcata
     4741 taatgtgaaa acgattttca taggcagaat aaataacatt taaattaaac tgggcatcag
     4801 cgcaattcaa ttggtttggt aatagcaagg gaaaatagaa tgaagtgatc tccaaaaaat
     4861 aagtactttt tgactgtaaa taaaattgta aggagtaaaa agtacttttt tttctaaaaa
     4921 aatgtaatta agtaaaagta aaagtattga tttttaattg tactcaagta aagtaaaaat
     4981 ccccaaaaat aatacttaag tacagtaatc aagtaaaatt actcaagtac tttacacctc
     5041 tggttcttga ccccctacct tcagcaagcc cagcagatcc actagttcta gagcggccgc
     5101 caccgcggtg gagctccagc ttttgttccc tttagtgagg gttaattgcg cgcttggcgt
     5161 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
     5221 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
     5281 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
     5341 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
     5401 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     5461 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     5521 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     5581 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     5641 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     5701 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     5761 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     5821 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     5881 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     5941 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     6001 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     6061 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     6121 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     6181 cggggtctga cgctcagtgg aacgaaaact cacgttaagg cattttggtc atgagattat
     6241 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     6301 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     6361 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     6421 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     6481 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     6541 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     6601 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
     6661 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     6721 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     6781 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     6841 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     6901 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
     6961 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     7021 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     7081 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     7141 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     7201 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     7261 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccaccta
     7321 aattgtaagc gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt
     7381 ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat
     7441 agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa
     7501 cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca cgctggctaa gaactcatca
     7561 gcctccccgg tccatctacc cacgtaccaa tgcaccaatt ggccacaatg acggctacta
     7621 catggtgcca ttccttcctc tttataggaa tggagactac ctcctgtcca acaatgctct
     7681 tggatacgag tacgcctacc tgttggaccc aggtcattgc acaacaccag aaatgccctc
     7741 tgatctgcaa aagacgtgaa tatctgttca gacacccata tccactctgt tccacacagg
     7801 tcagaggttt gtccaggagt tcttgacaga ggtgtaaaaa gtactcaaaa attttactca
     7861 agtgaaagta caagtactta gggaaaattt tactcaatta aaagtaaaag tatctggcta
     7921 gaatcttact tgagtaaaag taaaaaagta ctccattaaa attgtacttg agtattaagg
     7981 aagtaaaagt aaaagcaaga aagaaaacta gagattcttg tttaagcttt taatctcaaa
     8041 aaacattaaa tgaaatgcat acaaggtttt atcctgcttt agaactgttt gtatttaatt
     8101 atcaaactat aagacagaca atctaatgcc agtacacgct actcaaagtt gtaaaacctc
     8161 agatttaact tcagtagaag ctgattctca aaattgttag tgtcaagcct agctcttttg
     8221 gggctgaaaa gcaatcctgc agtgctgaaa agcctctcac aggcagccga tgcgggaaga
     8281 ggtgtattag tcttgataga gaggctgcaa atagcaggaa acgtgagcag agactccctg
     8341 gtgtctgaaa cacaggccca tttacgcctt aagatacatt gatgagtttg gacaaaccac
     8401 aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt
     8461 tgtaaccatt ataagctgca ataaacaagt taacaacaac aattgcattc attttatgtt
     8521 tcaggttcag ggggaggtgt gggaggtttt ttaaagcaag taa
//
