LOCUS       sequence-31313-e         884 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 884)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 884)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PRDM2
FEATURES             Location/Qualifiers
     source          1..884
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             60..77
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             87..104
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             575..592
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(645..663)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      659..706
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(830..849)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 cgacattccc tctgaatatt ttgtttactt taagaaggag atataccatg ggcagcagcc
       61 atcatcatca tcatcacagc agcggcctgg ttccgcgtgg tagtaatcag aacactactg
      121 agcctgtggc ggccaccgag accctggctg aggtacccga acatgtgctg cgaggacttc
      181 cggaggaagt gaggcttttc ccttctgctg ttgacaagac ccggattggt gtctgggcca
      241 ctaaaccaat tttaaaaggc aaaaaatttg ggccatttgt tggtgataag aaaaaaagat
      301 ctcaggttaa gaataatgta tacatgtggg aggtgtatta cccaaatttg ggatggatgt
      361 gcattgatgc cactgatcca gagaagggaa actggctgcg atatgtgaat tgggcttgct
      421 caggagaaga gcaaaattta ttcccactgg aaatcaacag agccatttac tataaaactt
      481 taaagccaat cgcgccgggc gaggagctcc tggtctggta caatggggaa gacaaccctg
      541 agatatgatg acgaagcttg cggccgcact cgagcaccac caccaccacc actgagatcc
      601 ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact
      661 agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac
      721 tatatccgga ttggcgaatg ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
      781 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
      841 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtca
//
