LOCUS       sequence-31314-e         923 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 923)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 923)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PRDM4
FEATURES             Location/Qualifiers
     source          1..923
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             58..75
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             85..102
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             585..602
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(655..673)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      669..716
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(840..859)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 gaattccctc tgaatatttt gtttacttta agaaggagat ataccatggg cagcagccat
       61 catcatcatc atcacagcag cggcctggtt ccgcgtggta gtgaacatgg accagtgact
      121 tttgttcctg acactccaat agagagcaga gcaaggcttt ctctcccaaa gcagcttgtt
      181 ctccgtcagt caattgtggg agcagaagtt ggtgtatgga ctggagaaac cattcctgtg
      241 cggacttgct ttggacctct aattggccag cagagtcact ccatggaagt agcagaatgg
      301 acagacaagg cagttaacca tatctggaag atataccaca atggtgtcct agaattctgc
      361 atcattacaa ctgatgaaaa tgaatgtaat tggatgatgt ttgtgcgcaa agccaggaac
      421 cgggaagagc agaatttggt ggcttatcct catgatggaa aaatcttttt ctgcacctca
      481 caagatatcc ctcctgaaaa tgaactgctt ttttattata gccgagatta tgctcaacag
      541 attggtgttc ctgaatgatg acgaagcttg cggccgcact cgagcaccac caccaccacc
      601 actgagatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg
      661 agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga
      721 aaggaggaac tatatccgga ttggcgaatg ggacgcgccc tgtagcggcg cattaagcgc
      781 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
      841 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
      901 aatcgggggc tccctttagg gtc
//
