LOCUS       sequence-313143-       19032 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expresses ß--glucuronidase in cells expressing ZmSWEET13a.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 19032)
  AUTHORS   Bezrutczyk M, Zollner NR, Kruse CPS, Hartwig T, Lautwein T, Kohrer 
            K, Frommer WB, Kim JY
  TITLE     Evidence for phloem loading via the abaxial bundle sheath cells in 
            maize leaves.
  JOURNAL   Plant Cell. 2021 May 5;33(3):531-547. doi: 10.1093/plcell/koaa055.
  PUBMED    33955497
REFERENCE   2  (bases 1 to 19032)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Plant Cell"; date: "2021-05-5- 5"; volume: "33"; issue: "3"; pages:
            "531-547"
COMMENT     Sequence Label: pZmSWEET13a:ZmSWEET13a:GUS
FEATURES             Location/Qualifiers
     source          1..19032
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    2502..2526
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             3857..4486
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             4915..5988
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      6054..6248
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(6484..6506)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    6592..6732
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     6647..6666
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(6747..6764)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6918..7506)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6998..7017)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(7752..8543)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     misc_feature    9064..9088
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(9181..9433)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(9453..10004)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(10025..10370)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(10062..10082)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             complement(11600..11617)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ctgtaggggg cactctacgg ccgcagcggt gccccctaca gcgcccccta cacgtcggcc
       61 cccttctctc cccccagatc tagcgtgtca ctattcatcc ctctaaacac tatgatatga
      121 tgtaggtcta cgagtaattg ttagtagata aaaaattgat agttgatata gaaaatgata
      181 ttttctagtt gtagtggggt atagggggag tatttagcgg gaaccgctgc gggagatgaa
      241 aaaatagggg gaaaataggg ggaaaaagtg atatagagga aaaaatttag aggtaacggt
      301 tgtggatagc ctaacccccc tcccctttag tactcgatcc acaaacacat gtactaaaga
      361 ccactcagcc atgaaattaa aactaaagat ttatgtcttt agtcctagtt gtctagtacc
      421 aaatatgaat acaatacaat agccgatttt caatcggtat tgatggccct ttctactgta
      481 ataggttaaa tcttctaaca acagcaagat aacattctta atttgattgt gattttgctc
      541 ctgagtagaa cctaatgata aattagaata ttctttttgg tcatgtaatt aaacatgaaa
      601 attaagttgt tctaaccact ggttaaacat tgttatgtgt attaccgagt ttttcaccat
      661 gaaatatttt ttgcagctta aaaacacccc atgtgttgct tcaaatgaat aatatagcat
      721 caaatatggt tgtacacggc accagtaact gcgactcaac aacagtagat cttcgtcggc
      781 atgtatagat cattcggaat gcctttgctt ctctaattct actttcctca cgtcgctgcg
      841 gttagtggac atacatagct ttatgccagc ttttattttt ctatattgcg tcgtttcaaa
      901 tcaattcaat gattagaaca ctaagcatat ataattaaac atattaaagt ttacgcgtgc
      961 tcctaagtaa catacatgtg atactaatta tttatggtgt cgtgtgaaaa aaaaactttt
     1021 tctttaatgt ttacatatat ttttcataaa attgtccttt tctcacagta aaataccgtc
     1081 aaaaggccat gatacagtta atcaagtaaa aatatttata gaatgaatac aatttatgta
     1141 gattatagga acacatattg taaaagtatg gtcaaagcgg aagtttaaag cgctagctgt
     1201 gcccaagttt tttcatgtag cttgacgaat gcgagaggag agagagtgtt ccacgtacgt
     1261 tcctcttcct aatcacgatt gttggaatca aactagagtg gttaattaaa atgatcattg
     1321 atgcagcatc agcaaaggat gttttcagct ataaatggtt gacgtttgga cagctcattt
     1381 caatgatatc ttgattcatg gcgtcaaaac ggcatgtatt cattcatgac tttgtatatt
     1441 actcctgccc tcataaaata gtactccacc cattctaaaa tatagttctt tctagtctac
     1501 tcttttcacc cacattcatt caaatgataa tgaatataga catatacata caaactgcat
     1561 tcatatatta cttaatgaat gcatgattag tttaaaatga attatatttt ggatcgggac
     1621 gaaatattcg ttttaggtgt tagttttttt atctatattc aaatggatga taatgaatct
     1681 agagacatat ataaaacaca tacatcaagt attgtatgaa tccattaatt atctaatacg
     1741 aattttattt aaatttggga cagagagagt attaggagtt gtgtgtgagc tgttcttggt
     1801 tttcacctct gctttgttct tggtgtaaaa agggtgtgca ttaattatcc tgtttgaagc
     1861 attataagac aaacgatgca ggacatcttt gcatgagagt catacacacg ggaggcaaat
     1921 atttcgtaca taaattatac aggaagaaga gaaccggatg caaatggtca aggataattt
     1981 aaggattgcg tacagtcacg ataaaagagt tataccgata ataggagaag agatttagct
     2041 tttgaggtag gagaatttgt atatcttaag gtgtcaccta tctaaggctt acgcaggatt
     2101 gaagtaaagg aaaaaatatc accagagtac gttagtacgt tcacaatctt agaatgaaaa
     2161 aatgaagtag cataccagtt gaagttgcat gagaggttag cagatgtaca tgacgtattt
     2221 catgtgtaat aattcaagtg gtggttgagg gtgctagagg aatagttgca aatgaagatc
     2281 taaatgttta tgatgatgtg acatgtacta agcccaatgg aaattttggg tacaactaag
     2341 agtggcaaaa atatgcaaaa ttaggtggag taaccacacc acatatcagg ctactaggta
     2401 aagagagaat aaccttaaag ccacataccc cgatcaggta agcttagctt gagcttggat
     2461 cagattgtcg tttcccgcct tcagtttaaa ctatcagtgt ttgacaggat atattggcgg
     2521 gtaaacctaa gagaaaagag cgtttattag aataatcgga tatttaaaag ggcgtgaaaa
     2581 ggtttatccg ttcgtccatt tgtatgtgca tgccaaccac agggttcccc tcgggatcaa
     2641 agtactttaa agtactttaa agtactttaa agtactttga tccaacccct ccgctgctat
     2701 agtgcagtcg gcttctgacg ttcagtgcag ccctcttctg aaaacgacat gtcgcacaag
     2761 tcctaagtta cgcgacaggc tgccgccctg cccttttcct ggcgttttct tgtcgcgtgt
     2821 tttagtcgca taaagtagaa tacttgcgac tagaaccgga gacattacgc catgaacaag
     2881 agcgccgccg ctggcctgct gggctatgcc cgcgtcagca ccgacgacca ggacttgacc
     2941 aaccaacggg ccgaactgca cgcggccggc tgcaccaagc tgttttccga gaagatcacc
     3001 ggcaccaggc gcgaccgccc ggagctggcc aggatgcttg accacctacg ccctggcgac
     3061 gttgtgacag tgaccaggct agaccgcctg gcccgcagca cccgcgacct actggacatt
     3121 gccgagcgca tccaggaggc cggcgcgggc ctgcgtagcc tggcagagcc gtgggccgac
     3181 accaccacgc cggccggccg catggtgttg accgtgttcg ccggcattgc cgagttcgag
     3241 cgttccctaa tcatcgaccg cacccggagc gggcgcgagg ccgccaaggc ccgaggcgtg
     3301 aagtttggcc cccgccctac cctcaccccg gcacagatcg cgcacgcccg cgagctgatc
     3361 gaccaggaag gccgcaccgt gaaagaggcg gctgcactgc ttggcgtgca tcgctcgacc
     3421 ctgtaccgcg cacttgagcg cagcgaggaa gtgacgccca ccgaggccag gcggcgcggt
     3481 gccttccgtg aggacgcatt gaccgaggcc gacgccctgg cggccgccga gaatgaacgc
     3541 caagaggaac aagcatgaaa ccgcaccagg acggccagga cgaaccgttt ttcattaccg
     3601 aagagatcga ggcggagatg atcgcggccg ggtacgtgtt cgagccgccc gcgcacgtct
     3661 caaccgtgcg gctgcatgaa atcctggccg gtttgtctga tgccaagctg gcggcctggc
     3721 cggccagctt ggccgctgaa gaaaccgagc gccgccgtct aaaaaggtga tgtgtatttg
     3781 agtaaaacag cttgcgtcat gcggtcgctg cgtatatgat gcgatgagta aataaacaaa
     3841 tacgcaaggg gaacgcatga aggttatcgc tgtacttaac cagaaaggcg ggtcaggcaa
     3901 gacgaccatc gcaacccatc tagcccgcgc cctgcaactc gccggggccg atgttctgtt
     3961 agtcgattcc gatccccagg gcagtgcccg cgattgggcg gccgtgcggg aagatcaacc
     4021 gctaaccgtt gtcggcatcg accgcccgac gattgaccgc gacgtgaagg ccatcggccg
     4081 gcgcgacttc gtagtgatcg acggagcgcc ccaggcggcg gacttggctg tgtccgcgat
     4141 caaggcagcc gacttcgtgc tgattccggt gcagccaagc ccttacgaca tatgggccac
     4201 cgccgacctg gtggagctgg ttaagcagcg cattgaggtc acggatggaa ggctacaagc
     4261 ggcctttgtc gtgtcgcggg cgatcaaagg cacgcgcatc ggcggtgagg ttgccgaggc
     4321 gctggccggg tacgagctgc ccattcttga gtcccgtatc acgcagcgcg tgagctaccc
     4381 aggcactgcc gccgccggca caaccgttct tgaatcagaa cccgagggcg acgctgcccg
     4441 cgaggtccag gcgctggccg ctgaaattaa atcaaaactc atttgagtta atgaggtaaa
     4501 gagaaaatga gcaaaagcac aaacacgcta agtgccggcc gtccgagcgc acgcagcagc
     4561 aaggctgcaa cgttggccag cctggcagac acgccagcca tgaagcgggt caactttcag
     4621 ttgccggcgg aggatcacac caagctgaag atgtacgcgg tacgccaagg caagaccatt
     4681 accgagctgc tatctgaata catcgcgcag ctaccagagt aaatgagcaa atgaataaat
     4741 gagtagatga attttagcgg ctaaaggagg cggcatggaa aatcaagaac aaccaggcac
     4801 cgacgccgtg gaatgcccca tgtgtggagg aacgggcggt tggccaggcg taagcggctg
     4861 ggttgtctgc cggccctgca atggcactgg aacccccaag cccgaggaat cggcgtgagc
     4921 ggtcgcaaac catccggccc ggtacaaatc ggcgcggcgc tgggtgatga cctggtggag
     4981 aagttgaagg ccgcgcaggc cgcccagcgg caacgcatcg aggcagaagc acgccccggt
     5041 gaatcgtggc aagcggccgc tgatcgaatc cgcaaagaat cccggcaacc gccggcagcc
     5101 ggtgcgccgt cgattaggaa gccgcccaag ggcgacgagc aaccagattt tttcgttccg
     5161 atgctctatg acgtgggcac ccgcgatagt cgcagcatca tggacgtggc cgttttccgt
     5221 ctgtcgaagc gtgaccgacg agctggcgag gtgatccgct acgagcttcc agacgggcac
     5281 gtagaggttt ccgcagggcc ggccggcatg gccagtgtgt gggattacga cctggtactg
     5341 atggcggttt cccatctaac cgaatccatg aaccgatacc gggaagggaa gggagacaag
     5401 cccggccgcg tgttccgtcc acacgttgcg gacgtactca agttctgccg gcgagccgat
     5461 ggcggaaagc agaaagacga cctggtagaa acctgcattc ggttaaacac cacgcacgtt
     5521 gccatgcagc gtacgaagaa ggccaagaac ggccgcctgg tgacggtatc cgagggtgaa
     5581 gccttgatta gccgctacaa gatcgtaaag agcgaaaccg ggcggccgga gtacatcgag
     5641 atcgagctag ctgattggat gtaccgcgag atcacagaag gcaagaaccc ggacgtgctg
     5701 acggttcacc ccgattactt tttgatcgat cccggcatcg gccgttttct ctaccgcctg
     5761 gcacgccgcg ccgcaggcaa ggcagaagcc agatggttgt tcaagacgat ctacgaacgc
     5821 agtggcagcg ccggagagtt caagaagttc tgtttcaccg tgcgcaagct gatcgggtca
     5881 aatgacctgc cggagtacga tttgaaggag gaggcggggc aggctggccc gatcctagtc
     5941 atgcgctacc gcaacctgat cgagggcgaa gcatccgccg gttcctaatg tacggagcag
     6001 atgctagggc aaattgccct agcaggggaa aaaggtcgaa aagctctctt tcctgtggat
     6061 agcacgtaca ttgggaaccc aaagccgtac attgggaacc ggaacccgta cattgggaac
     6121 ccaaagccgt acattgggaa ccggtcacac atgtaagtga ctgatataaa agagaaaaaa
     6181 ggcgattttt ccgcctaaaa ctctttaaaa cttattaaaa ctcttaaaac ccgcctggcc
     6241 tgtgcataac tgtctggcca gcgcacagcc gaagagctgc aaaaagcgcc tacccttcgg
     6301 tcgctgcgct ccctacgccc cgccgcttcg cgtcggccta tcgcggccgc tggccgctca
     6361 aaaatggctg gcctacggcc aggcaatcta ccagggcgcg gacaagccgc gccgtcgcca
     6421 ctcgaccgcc ggcgcccaca tcaaggcacc ctgcctcgcg cgtttcggtg atgacggtga
     6481 aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg
     6541 gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gcgcagccat
     6601 gacccagtca cgtagcgata gcggagtgta tactggctta actatgcggc atcagagcag
     6661 attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa
     6721 taccgcatca ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     6781 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     6841 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     6901 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     6961 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     7021 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     7081 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     7141 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     7201 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     7261 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     7321 ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct
     7381 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     7441 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     7501 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     7561 cgttaaggga ttttggtcat gcatgatata tctcccaatt tgtgtagggc ttattatgca
     7621 cgcttaaaaa taataaaagc agacttgacc tgatagtttg gctgtgagca attatgtgct
     7681 tagtgcatct aacgcttgag ttaagccgcg ccgcgaagcg gcgtcggctt gaacgaattt
     7741 ctagctagac attatttgcc gactaccttg gtgatctcgc ctttcacgta gtggacaaat
     7801 tcttccaact gatctgcgcg cgaggccaag cgatcttctt cttgtccaag ataagcctgt
     7861 ctagcttcaa gtatgacggg ctgatactgg gccggcaggc gctccattgc ccagtcggca
     7921 gcgacatcct tcggcgcgat tttgccggtt actgcgctgt accaaatgcg ggacaacgta
     7981 agcactacat ttcgctcatc gccagcccag tcgggcggcg agttccatag cgttaaggtt
     8041 tcatttagcg cctcaaatag atcctgttca ggaaccggat caaagagttc ctccgccgct
     8101 ggacctacca aggcaacgct atgttctctt gcttttgtca gcaagatagc cagatcaatg
     8161 tcgatcgtgg ctggctcgaa gatacctgca agaatgtcat tgcgctgcca ttctccaaat
     8221 tgcagttcgc gcttagctgg ataacgccac ggaatgatgt cgtcgtgcac aacaatggtg
     8281 acttctacag cgcggagaat ctcgctctct ccaggggaag ccgaagtttc caaaaggtcg
     8341 ttgatcaaag ctcgccgcgt tgtttcatca agccttacgg tcaccgtaac cagcaaatca
     8401 atatcactgt gtggcttcag gccgccatcc actgcggagc cgtacaaatg tacggccagc
     8461 aacgtcggtt cgagatggcg ctcgatgacg ccaactacct ctgatagttg agtcgatact
     8521 tcggcgatca ccgcttcccc catgatgttt aactttgttt tagggcgact gccctgctgc
     8581 gtaacatcgt tgctgctcca taacatcaaa catcgaccca cggcgtaacg cgcttgctgc
     8641 ttggatgccc gaggcataga ctgtacccca aaaaaacagt cataacaagc catgaaaacc
     8701 gccactgcgc cgttaccacc gctgcgttcg gtcaaggttc tggaccagtt gcgtgagcgc
     8761 atacgctact tgcattacag cttacgaacc gaacaggctt atgtccactg ggttcgtgcc
     8821 cgaattgatc acaggcagca acgctctgtc atcgttacaa tcaacatgct accctccgcg
     8881 agatcatccg tgtttcaaac ccggcagctt agttgccgtt cttccgaata gcatcggtaa
     8941 catgagcaaa gtctgccgcc ttacaacggc tctcccgctg acgccgtccc ggactgatgg
     9001 gctgcctgta tcgagtggtg attttgtgcc gagctgccgg tcggggagct gttggctggc
     9061 tggtggcagg atatattgtg gtgtaaacaa attgacgctt agacaactta ataacacatt
     9121 gcggacgttt ttaatgtact gaattaacgc cgaattgaat tatcagcttg catgccggtc
     9181 gatctagtaa catagatgac accgcgcgcg ataatttatc ctagtttgcg cgctatattt
     9241 tgttttctat cgcgtattaa atgtataatt gcgggactct aatcataaaa acccatctca
     9301 taaataacgt catgcattac atgttaatta ttacatgctt aacgtaattc aacagaaatt
     9361 atatgataat catcgcaaga ccggcaacag gattcaatct taagaaactt tattgccaaa
     9421 tgtttgaacg atctgcttga ctctaggggt catcagattt cggtgacggg caggaccgga
     9481 cggggcggca ccggcaggct gaagtccagc tgccagaaac ccacgtcatg ccagttcccg
     9541 tgcttgaagc cggccgcccg cagcatgccg cggggggcat atccgagcgc ctcgtgcatg
     9601 cgcacgctcg ggtcgttggg cagcccgatg acagcgacca cgctcttgaa gccctgtgcc
     9661 tccagggact tcagcaggtg ggtgtagagc gtggagccca gtcccgtccg ctggtggcgg
     9721 ggggagacgt acacggtcga ctcggccgtc cagtcgtagg cgttgcgtgc cttccaggga
     9781 cccgcgtagg cgatgccggc gacctcgccg tccacctcgg cgacgagcca gggatagcgc
     9841 tcccgcagac ggacgaggtc gtccgtccac tcctgcggtt cctgcggctc ggtacggaag
     9901 ttgaccgtgc ttgtctcgat gtagtggttg acgatggtgc agaccgccgg catgtccgcc
     9961 tcggtggcac ggcggatgtc ggccgggcgt cgttctgggc tcatctgcag gagctcgtcc
    10021 cccgtgttct ctccaaatga aatgaacttc cttatataga ggaagggtct tgcgaaggat
    10081 agtgggattg tgcgtcatcc cttacgtcag tggagatatc acatcaatcc acttgctttg
    10141 aagacgtggt tggaacgtct tctttttcca cgatgctcct cgtgggtggg ggtccatctt
    10201 tgggaccact gtcggcagag gcatcttcaa cgatggcctt tcctttatcg caatgatggc
    10261 atttgtagga gccaccttcc ttttccacta tcttcacaat aaagtgacag atagctgggc
    10321 aatggaatcc gaggaggttt ccggatatta ccctttgttg aaaagtctca attgcccttt
    10381 ggtcttctga gactgtatct ttgatatttt tggagtagac aagtgtgtcg tgctccacca
    10441 tgttgacgaa gattttcttc ttgtcattga gtcgtaagag actctgtatg aactgttcgc
    10501 cagtctttac ggcgagttct gttaggtcct ctatttgaat ctttgactcc atggcctttg
    10561 attcagtggg aactaccttt ttagagactc caatctctat tacttgcctt ggtttgtgaa
    10621 gcaagccttg aatcgtccat actggaatag tacttctgat cttgagaaat atatctttct
    10681 ctgtgttctt gatgcagtta gtcctgaatc ttttgactgc atctttaacc ttcttgggaa
    10741 ggtatttgat ttcctggaga ttattgctcg ggtagatcgt cttgatgaga cgtgctgcgt
    10801 aagcctctct aaccatctgt gggttagcat tctttctgaa attgaaaagg ctaatctggg
    10861 gaccgaatgg atcggattcg cggtacccgg ggatcctcta gagggcccat acgccaaatc
    10921 tgggagtagc cgcccgtggt gcctctagag gccgccaccg cgcttggctt cgcggtcgcg
    10981 cgagtctctg ggcgccgtcg cctttcgctc tcggggaact gccgtcgcct cgcgctctcg
    11041 ggtaaccgct gccacactcg cgtctgggtg ggcgttgtcg ctcatttata ggggtgggga
    11101 gtcaaaaact gaaacttagc aatttgaggc tccttttata aacgtccggg acctagtgtg
    11161 attgaaccgg atgacaactt tgggccaggg ctttcccgcc cgaacacaca gaagccattt
    11221 ttgggaaact tgtggtagct aacaacagta gtgtgggttc aattacattt tattagaaga
    11281 cgttacacat aaaaaggaat aacagatatc catgcaaatt aatacatagc tgcaaagatg
    11341 aacacaaagc ccatatatct atattaatta cacacatata tgtatagctt cccccttcat
    11401 cgttctggcc tctgcctcca tccattagct agcttccatg catggcagtt aattacttat
    11461 atatatacta cttaatcatt ttctccatca tatatatgct cacgaccagc agcaagtcgt
    11521 ccttcgaata tataaaagct agcgtacgtg tggcgggcgc atgcatgggc catgcacgcg
    11581 atcgatcgag aatctacacg tgatggtgat ggtgatggct agcgttcttg tagccgaaat
    11641 ctggaatgtt ggtccagcgc tcgcgaaaga cgtgcgcggc gagcttcggc ttgcggtcac
    11701 gagtgaacac gcccttcttg tttccttgga cgcgcatcac gccctgagag gtcgcgaagt
    11761 ccgcgaagtt ccacgcttgc tcacccacga agttctcaaa ctcatcgaac acgacgtggt
    11821 tcgcctggta gtactcgact tgatattcct cggtgaacat cactggatca atgtcgtgaa
    11881 agcccgcaac ggtgtctgcg ccgtactcag tgatcatgat cggctttcct gggcaacgct
    11941 tgttccacgc gtgaaattcc tggcggagat ggactttggc cgcttcgaga tcaccgccat
    12001 cgaagtacca tccgttatag cgattgagcg cgatgacgtc aatcagttcg gcgactttgt
    12061 ccgtctccgg ggtagccatc acaaacagca cgatcgtgac cggacgcttc tgtgggtcga
    12121 gttccttggt cagctccacc aacggcttga agtactcgta cgcgccctct tcctcagtcg
    12181 ccgcctcgtt ggcgatgctc cacatcacga cgcttggatg gttcttgtca cgagacacca
    12241 gttcacggag aacgtcttga tggtgctcaa acgtccgaat cttctcccag gtactgacgc
    12301 gctcgctgcc ttcgccgagt cccgtggtgg ccatgaagtt gaggtgcacg ccaactgccg
    12361 gagtctcgtc gatcacgacc agaccctcgc gatccgcaag acgcatcaac tcttcagagt
    12421 acggatagtg tgcggtccgg aagctgttgg cgccgatcca tttgaggata ttgaaatcca
    12481 tcacattgct cgcttcgtta aagccacggc cgttgatagg agtgtcctca tgtttgccaa
    12541 agcccttgaa gtagaacggt ttgttgttga tgaggaactt gccgtcgttg acttccacgg
    12601 tccgcacgcc gaacggctct tcatagacat cgatggtcag tccgtcgttc accagttcca
    12661 ctttgatctg gtagagatac gtgttcagtg gttcccagag gatgacattc ggaatctcca
    12721 cgttaccgct caggccctcg gtgcttgcga ccactttgcc ttcctcatcc acgaccgaca
    12781 ctttcacggt ctcggctttg ccttgaaagt ccaccgtata ggtcacagtc ccggttgggc
    12841 cattgaagtc ggtcacaacc gagatgtcct cgacgtacgt aaacggggtc gtgtagattt
    12901 tcaccggacg gtgcaggcct gcatagttga agaagtcgaa gttcggcttg ttacgaatga
    12961 cttttccgag gccctcttcg tggcgctcgc tgtacagccc caccgggagg gtgctatcgt
    13021 cgaggatgtt gtccacggcg acggtgacgc gattcatgcc atcacgcagc gagttgttga
    13081 tttccgcttc gaatggcagg aatccgccct tgtgctccac gaccagctca ccattgacat
    13141 agacaattgc tttgtgagtt gcagagccga agcggagcac gatacgctga tccttcagat
    13201 aggccggcac cgtgaactca cgttcgtacc agacatatcc gatatggttg cggatttcct
    13261 tggtcacgcc aatgtcattg taactgcttg ggacggccat actaatagtg tcggtcagct
    13321 tgctttcgta ccacttctct tccagtcctt tcccgtagtc cagcttgaag ttccagacgc
    13381 cattgaggtc gaagacgcca cgggtctcgg tgttgatcgg gtacagacta gttcgtcggt
    13441 tggcggcagc agccgctgcg gcggctgctg ccacgtggac ggcggggctg tgtcggctga
    13501 cgaagacgtc gaccacgtcc ctgttgggca gcatcgccgg cgcggttgct tcagcagcgc
    13561 agctcctgac aggaacgccc gccatctggg tgactgggtg caccccggag ctcctctccg
    13621 ggagggctgg gctgagcttg gcgatgttga cgaggacgtg ctcgtctgca gctgaggaaa
    13681 gcttgccggc atccttgccc tcggcagtcg ccgccaccgg cgtcttgttc atgtacaaca
    13741 cgtagagcac catctggacc acgccgaagg tgaaccccag gatgtttgga agctgcatac
    13801 gtacagagat cgtatgtagt ttatcagtaa agaccagtga gagataccaa taccatggcg
    13861 gttgcagcag attagatgaa acaattaccg cgacgtattt gtccttgatg aggaggccgt
    13921 agaggaacca gacgacggcg ctgagggtga gcgagaggga gagggagaag ggcatgtact
    13981 ccacgctctt cgtctggatc actcgtctct gcacacatat ataaacacgg tgcattagaa
    14041 acgatacaca tggatcaaaa ctgattgggt tgatcgatct gtcgtccatg catcttggca
    14101 cagttttata cgcgtgctca gggctcacca tgatgctgag tggcgcgacg aagacactga
    14161 cggagaagcc gacgcagatc cagccaagca gcacaacgcg cttactgccc ttgaagagga
    14221 gaagggtgag caggaggatg accccaaaga cgccgccatt gaggaggacc atgatcttgg
    14281 ccgtgaacag cttggctttc ttgggcgcgt agacgaagta catgacgacg tagatggtct
    14341 cgatgacgca gccggcggcg ttgatggtga tgaggaaggt ctcgttggac ttgatcagtg
    14401 cgtagaagat ccacagcatg gcgctgaaca gggcaaccac gtagggaacc gactggaagc
    14461 cttccgtcga cttgctcttg tagatgcggt agaacgtcgg tctgttatac gatgcataca
    14521 tatccacata attaattaaa ataaccgatg aatatatatc tgaagaagca gtgataatta
    14581 attaagcaat atatatatat atatatgtca cttacatcgg ggccaggaag gtcatgaagg
    14641 agatgacgtt acctgcaaag caaggagaaa aaaaaggcgc caagatcagc catttgtatg
    14701 gcaaaagtgt gcccaaataa agcagagata agttgtgata agcagagctg acactttaca
    14761 cctccgtggc ctgtctaatt tgccttgttt tgtgactaaa gattcgtgag gctttattgt
    14821 ttgtataaag acagatgcct atctaaacac tctaaataaa ggaccgattg ctagctcgac
    14881 aggtagttca tgacggaaat gaataggata aactaaagga tcgaccatgg atggcagagg
    14941 taatacagta cataaaatct agtggtgcat gcttaatctg gtgcaaaact aagtgattgt
    15001 gagcgcaaga tcgagcgtct tgtttttggg attgttttgg aaggtatatc tcaaagtggc
    15061 ttctatgaag ggtatagatt gattctatgc atatgtgcac gaaggaggcc tgcttgttgt
    15121 gcatataata tatacttaca tagtagtata tatatgtaaa gagaaaacaa attaaacaga
    15181 atagaaagat aaatatataa tatatacatg cgcaataatt aagggcctgc ttagttcgtg
    15241 gctaaccatg tcacactttg gctaagatta atcgttcgaa ttgaagaact ataaccttag
    15301 acagaaaagt tagacaaagt atggtaaaat aggtagcaaa ccaaacatgc ctgcccttaa
    15361 ttcatagctt tgcggcgggc gcttgtttag ctattagtga aggtgtagta tgtacctagt
    15421 agaccgaaag caaacgccca ggggtgttgc agagacatgc cagccatttg atgggtacaa
    15481 aggggggaat gaagctagta gctacacgga taataatata acaggggtca caagaatttc
    15541 ccaacacaca aagcagttgc ttggattagg gtgatgagga gatcgagctt gtttttcttg
    15601 tgttgggcta gggaactact tctcctactc tctaaccctt ttggccccta caggagggaa
    15661 gggagagttg tgaggagttg tcctctctct gtgtgtgttg gctcaaactc ttgcaatgat
    15721 aggcagggct gtgggtggct ttatataggc acgcgaggtg gcacatgcac gcatgatttg
    15781 gtgtacatgt gttgtgtcat cgtgtacaag caagctcagg gctcttcaat cacacacata
    15841 tgatattaac actttttttt gtgaaattat atatagacgc aaagatcctt ttcaatgcat
    15901 gtatatatat agcagcttta taatagatga catttttttc tggccatgta tatatataac
    15961 cagagtgtat aaattgttac attaattgat tgagataatc ttctacattt tcgaaaaaag
    16021 cgggagctgc tttgcgtgca tgctttttag tcaattatac atcggtcagt gccagagtcg
    16081 tacactacaa attgttgtgc accgcgtggg gttctcgatc tccgacctgc tttggactct
    16141 gtggtaaggg ccgtctctgg taaaggaact tttgtacact atacaaattg ttgtgcacta
    16201 aagtaaatta aatcaaatta gtacctatta ttgcattaaa atcggtactc actcaattcg
    16261 tcagcaccgg ttttaactaa tgcgtcctcg tagagctagg gcattaatac tgattaatgt
    16321 ctctaaccgg aactaatatt aacttatcac tatcggttga cgacaccaat tggtactaat
    16381 ataattctat tagtaccgat tggtgttttt aactggaatt tgatgacata acattattat
    16441 cgattggtgt tttcaactag tgttaatgta cctgctttat aacctccaac cctcgctccc
    16501 cgagctggcg ttaggaacaa agaggagctt agtgtaataa gcttcaagta gagattgtaa
    16561 cttgctttgt tttgtctacc atcgctctaa tgaacgctcg tagtttagtt tgccatctct
    16621 ctaaagaact ctttagtatt ggttcttaca cgaacggtac taaaggttct ttttcaattc
    16681 catcctgaag gtctttagca ttggttcttt gtaagaactg gtattaatgg cccctttcca
    16741 gttcttggtc agaggtcttt agtactggtt ctttgcaaga atcgataagt gcccaatata
    16801 acttcagcct tcgttatgcc cttcttcttc tcttcttcta gcacctttct tggtgagctc
    16861 gtcgagccct ctccccacac caccatttcc catgtcgagc atacatgcaa gggccctcct
    16921 cgcgccacca cttctcccat cgaccctgca ccatcgccat agaagtgact ccctaaccct
    16981 acaatgagca cattagaaac cgaagctttg ttatatcatt gcaatatatc tatctatctt
    17041 tataattata tataatcatg gtgtattaaa ctcatggaaa gattattgtg tgtctttgct
    17101 atagaagatt cttatgtgcc tttgctatat agtttccctt ttccacgatc acttgcaagc
    17161 ttgatgtata gaaattgttg tttctctctc ttttttcttt tggaccataa tgcccacaat
    17221 gcgtatatag agccacattt ctccaatgaa cataatacat tatcaagaaa gtcgtgattc
    17281 gaagtagttc gatataatgt tattttaaaa aggacattca ttcttgagct caaccttaaa
    17341 ccctattttt agttctatat attttgtggg agatagatat cctatgggtc gccaaggcat
    17401 aaatgtactc cttgggccat gggagaggac cctaataaga cgtccggtgg ggcgtactga
    17461 tgggcctcgt cctgacgaaa taaaagtcaa atgcagcaac ggagcactgg atacgaggag
    17521 atctcgaaga acactgcacc aacataccat ccgtgcacgt atatcgcata agaattacca
    17581 aatttctgaa gacacgtttc tcacgcggct aggtacttga taactcggat gataaaccag
    17641 agaagccaac caagcaccgg aaaaacaggc atactcatag caatagaaag tagatttaga
    17701 taagctcgat gaaatttaca ggtataactc tccagtcgac tatataaggc agtagagata
    17761 cccctaccaa aaactctctc tctcactctc gttcaccatt agattgattg gagaccttat
    17821 cttccgcccc taaaccacct gtaatttgtg aactcaaggc aattcaatac tcatcaccac
    17881 acatgacgta gggtattaca cattcacgtg gcccgtactt gtataaactt cctgtgttct
    17941 ggcatgctcc tgtacatacc atcgagccgc gatcgacgtc accgtcctcc caaaagcact
    18001 gcgtgggcaa ccccgtagtg cgctatcggg actaaacatc gacatatttt tcataggaat
    18061 catgctaaga tggatcgcca aataatggag aagttaaatc atgaacgaac ccataagcag
    18121 gaaactagcc cttagggcta catgtgttgt tccattaaac tagcctttcg aactatgagt
    18181 gatgttacag taatctagcc cttagggcta tgagtgatgc ctaagttgga tgcacctttt
    18241 tatttcttat actaagggaa actttatcca taaggtatgt tgctgcaaca atgtctcctt
    18301 gcacatcttt gtttttgaac gttgtcatca acaccgaggt aatggaacat tgccatatat
    18361 acaaatcgaa caataagaaa gaacacataa ttgaatgttg ccgtcagcgc cgagttaaag
    18421 gaacatttca agaagaaatt atctatgaag gaagcaccaa tagactcctc cttagcaaag
    18481 tctacaatat taatgttgat tttatgataa taaagtgtct tagctttgaa tttaaaatta
    18541 ttattaattt tttgataaat catagtttat gaaaacgaag gaagttcctt aggaaaagat
    18601 tgaaacaatt caaggacaac taatatggtt tatatataat aagatcgtat cgaaaaacga
    18661 agagttctat tatgatggac gaaatctttt ggtcaaatga gaaggcgaag atgatgaata
    18721 ttgagaatat agaaacgaat aaatatttaa tactcagtaa acatcatatt atttgttgtt
    18781 acatatgtat acatttaata ttaatattca tgaactagga ctattaataa atttgtaaat
    18841 attaatgtac atatattaat taaagcatat atattaaatg tttatcaatt aatatttttt
    18901 aaatgtacat aataaaagtt ccctagacac tataatgtgc cttgaaccag tactaaaagg
    18961 tcccaatagg agcgcacact aagactaact gcaacgacag cccctacgca gccccctccc
    19021 cttgcatgcg gc
//
