LOCUS       sequence-313148-       10254 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of synaptophysin 1 variant with self-cleaving c-terminal 
            tail. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10254)
  AUTHORS   Vevea JD, Chapman ER
  TITLE     Acute disruption of the synaptic vesicle membrane protein 
            synaptotagmin 1 using knockoff in mouse hippocampal neurons.
  JOURNAL   eLife. 2020 Jun 9;9. pii: 56469. doi: 10.7554/eLife.56469.
  PUBMED    32515733
REFERENCE   2  (bases 1 to 10254)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "eLife."; 
            date: "2020-06-9"; volume: "9. pii: 56469. doi"; pages: " 
            10.7554/eLife.56469"
COMMENT     Sequence Label: pF(UG) hSyn SYP-SELF
FEATURES             Location/Qualifiers
     source          1..10254
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      479..488
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2276..2299
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    2345..2933
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2398..2418)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2816..2827)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(2936..2952)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2937..2953)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             3458..3638
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(3664..3681)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3670..3894
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3940..4368
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4027..4046)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4237..4258
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4377..4397)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4382..4711
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4562..4697
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4624..4643
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        4759..4806
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             4825..5199
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    5329..5450
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5366..5385)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5420..5439
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5499..5515)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5499..5515)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5512..5534)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5523..5539
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5547..5553,5554..5571,5572..5577))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5592..5613
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5730..5747)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5901..6489)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5981..6000)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6660..7451,7452..7520))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7283..7302
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7521..7625)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(7700..7719)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        7891..8270
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8272..8470
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     8425..8445
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             8488..8668
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8715..8840
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9333..9566
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9751..9795
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    10093..10210
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 agtgcaagtg gttttaggac caggatgagg cggggtgggg gtgcctacct gacgaccgac
       61 cccgacccac tggacaagca cccaaccccc attccccaaa ttgcgcatcc cctatcagag
      121 agggggaggg gaaacaggat gcggcgaggc gcgtgcgcac tgccagcttc agcaccgcgg
      181 acagtgcctt cgcccccgcc tggcggcgcg cgccaccgcc gcctcagcac tgaaggcgcg
      241 ctgacgtcac tcgccggtcc cccgtaaact ccccttcccg gccaccttgg tcgcgtccgc
      301 gccgccgccg gcccagccgg accgcaccac gcgaggcgcg agataggggg gcacgggcgc
      361 gaccatctgc gctgcggcgc cggcgactca gcgctgcctc agtctgcggt gggcagcgga
      421 ggagtcgtgt cgtgcctgag agcgcaggta cggctagcgg atccggtacc gcgggcccgc
      481 caccatggac gtggtgaatc agctggtggc tgggggtcag ttccgggtgg tcaaggagcc
      541 ccttggcttc gtgaaggtgc tgcagtgggt ctttgccatc ttcgcctttg ctacgtgtgg
      601 cagctacacc ggggagcttc ggctgagcgt ggagtgtgcc aacaagacgg agagtgccct
      661 caacatcgaa gttgaattcg agtacccctt caggctgcac caagtgtact ttgatgcacc
      721 ctcctgcgtc aaagggggca ctaccaagat cttcctggtt ggggactact cctcgtcggc
      781 tgaattcttt gtcaccgtgg ctgtgtttgc cttcctctac tccatggggg ccctggccac
      841 ctacatcttc ctgcagaaca agtaccgaga gaacaacaaa gggcctatga tggactttct
      901 ggctacagcc gtgttcgctt tcatgtggct agttagttca tcagcctggg ccaaaggcct
      961 gtccgatgtg aagatggcca cggacccaga gaacattatc aaggagatgc ccatgtgccg
     1021 ccagacaggg aacacatgca aggaactgag ggaccctgtg acttcaggac tcaacacctc
     1081 agtggtgttt ggcttcctga acctggtgct ctgggttggc aacttatggt tcgtgttcaa
     1141 ggagacaggc tgggcagccc cattcatgcg cgcacctcca ggcgccgctg acgtggtttg
     1201 ctgtcagatg agttatccgg aaaagcaacc agcacctggc gatgcctacg gcgatgcggg
     1261 ctacgggcag ggccccggag gctatgggcc ccaggactcc tacgggcctc agggtggtta
     1321 tcaacccgat tacgggcagc cagccagcgg tggcggtggc tacgggcctc agggcgacta
     1381 tgggcagcaa ggctatggcc aacagggtgc gcccacctcc ttctccaatc agatgaaagg
     1441 ttcagggtct agtggcagct ctggatcctc ggggtcagca ggcagtgctg ccggttccgg
     1501 aagcgggtcc agtggtagct caggctcttc cggaagcgga tccggaagtt cggggtccag
     1561 cggttctagt ggttcagccg gaagcgccgc tggaagtggg tcaggatcat ctggcagatc
     1621 ggggagtagc ggttcctcag gctgcgtggt gatcgtgggc aggatcgtgc tgagcggaag
     1681 cggcaccagc gcccccatca ccgcctacgc ccagcagacc aggggcctgc tgggctgcat
     1741 catcaccagc ctgaccggca gagacaagaa ccaggtggag ggcgaggtgc agatcgtgag
     1801 caccgccacc cagaccttcc tggccacctg catcaacgga gtgtgttgga ccgtgtacca
     1861 cggcgccggc accagaacca tcgccagccc caagggcccc gtgatccaga tgtacacaaa
     1921 cgtggaccag gacctggtgg gctggcccgc cccccagggc agcaggagcc tgaccccctg
     1981 cacatgcggc agcagcgacc tgtacctggt taccaggcac gccgacgtga tccccgtgag
     2041 gaggaggggc gacagcaggg gcagcctgct gagccccagg cccatcagct acttgaaggg
     2101 atctagcgga ggccccctgc tgtgccccgc cggccacgcc gtgggcctgt tcagggccgc
     2161 cgtgtgcacc aggggcgtgg ccaaggccgt ggacttcatc cccgtggaga acctggagac
     2221 caccatgagg agccccgtgt ttaccgacaa ctcctctcca ccagcaggat ccagtgacta
     2281 caaggatgac gacgataaat aaggccgcac tcctcagcta gaattcgata tcaagcttat
     2341 cgataatcaa cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt
     2401 tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc
     2461 ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga
     2521 gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc
     2581 cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct
     2641 ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg
     2701 gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct
     2761 gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc
     2821 cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg
     2881 tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcatcgata
     2941 ccgtcgacct cgagacctag aaaaacatgg agcaatcaca agtagcaata cagcagctac
     3001 caatgctgat tgtgcctggc tagaagcaca agaggaggag gaggtgggtt ttccagtcac
     3061 acctcaggta cctttaagac caatgactta caaggcagct gtagatctta gccacttttt
     3121 aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag atatccttga
     3181 tctgtggatc taccacacac aaggctactt ccctgattgg cagaactaca caccagggcc
     3241 agggatcaga tatccactga cctttggatg gtgctacaag ctagtaccag ttgagcaaga
     3301 gaaggtagaa gaagccaatg aaggagagaa cacccgcttg ttacaccctg tgagcctgca
     3361 tgggatggat gacccggaga gagaagtatt agagtggagg tttgacagcc gcctagcatt
     3421 tcatcacatg gcccgagagc tgcatccgga ctgtactggg tctctctggt tagaccagat
     3481 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     3541 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     3601 cctcagaccc ttttagtcag tgtggaaaat ctctagcagg gcccgtttaa acccgctgat
     3661 cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt
     3721 ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat
     3781 cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg
     3841 gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg
     3901 aggcggaaag aaccagctgg ggctctaggg ggtatcccca cgcgccctgt agcggcgcat
     3961 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
     4021 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
     4081 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
     4141 ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt
     4201 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
     4261 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg
     4321 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa
     4381 tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
     4441 catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag
     4501 aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc
     4561 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
     4621 ttttatttat gcagaggccg aggccgcctc tgcctctgag ctattccaga agtagtgagg
     4681 aggctttttt ggaggcctag gcttttgcaa aaagctcccg ggagcttgta tatccatttt
     4741 cggatctgat cagcacgtgt tgacaattaa tcatcggcat agtatatcgg catagtataa
     4801 tacgacaagg tgaggaacta aaccatggcc aagttgacca gtgccgttcc ggtgctcacc
     4861 gcgcgcgacg tcgccggagc ggtcgagttc tggaccgacc ggctcgggtt ctcccgggac
     4921 ttcgtggagg acgacttcgc cggtgtggtc cgggacgacg tgaccctgtt catcagcgcg
     4981 gtccaggacc aggtggtgcc ggacaacacc ctggcctggg tgtgggtgcg cggcctggac
     5041 gagctgtacg ccgagtggtc ggaggtcgtg tccacgaact tccgggacgc ctccgggccg
     5101 gccatgaccg agatcggcga gcagccgtgg gggcgggagt tcgccctgcg cgacccggcc
     5161 ggcaactgcg tgcacttcgt ggccgaggag caggactgac acgtgctacg agatttcgat
     5221 tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg
     5281 atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccccaa cttgtttatt
     5341 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     5401 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgt
     5461 ataccgtcga cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga
     5521 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
     5581 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
     5641 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
     5701 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     5761 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     5821 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     5881 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     5941 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     6001 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     6061 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     6121 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     6181 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     6241 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     6301 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
     6361 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     6421 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     6481 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     6541 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     6601 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     6661 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     6721 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     6781 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
     6841 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     6901 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     6961 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     7021 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     7081 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     7141 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     7201 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     7261 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     7321 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     7381 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     7441 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     7501 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     7561 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     7621 ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg gatcgggaga tctcccgatc
     7681 ccctatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatctgc
     7741 tccctgcttg tgtgttggag gtcgctgagt agtgcgcgag caaaatttaa gctacaacaa
     7801 ggcaaggctt gaccgacaat tgcatgaaga atctgcttag ggttaggcgt tttgcgctgc
     7861 ttcgcgatgt acgggccaga tatacgcgtt gacattgatt attgactagt tattaatagt
     7921 aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta
     7981 cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga
     8041 cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt
     8101 tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta
     8161 ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg
     8221 actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt
     8281 tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc
     8341 accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat
     8401 gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct
     8461 atataagcag cgcgttttgc ctgtactggg tctctctggt tagaccagat ctgagcctgg
     8521 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
     8581 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
     8641 ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag
     8701 ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag
     8761 gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc tagaaggaga
     8821 gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga tgggaaaaaa
     8881 ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta tgggcaagca
     8941 gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa ggctgtagac
     9001 aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt agatcattat
     9061 ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa gacaccaagg
     9121 aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg
     9181 ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag tgaattatat
     9241 aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc aaagagaaga
     9301 gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg gttcttggga
     9361 gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc cagacaatta
     9421 ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc gcaacagcat
     9481 ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct ggctgtggaa
     9541 agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa actcatttgc
     9601 accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca gatttggaat
     9661 cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt aatacactcc
     9721 ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt ggaattagat
     9781 aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta tataaaatta
     9841 ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt actttctata
     9901 gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct cccaaccccg
     9961 aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga cagagacaga
    10021 tccattcgat tagtgaacgg atcggcactg cgtgcgccaa ttctgcagac aaatggcagt
    10081 attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat
    10141 agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat
    10201 tcaaaatttt cgggtttatt acagggacag cagagatcca gtttggttaa ttaa
//
