LOCUS       sequence-31317-e         897 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 897)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 897)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: CBX4
FEATURES             Location/Qualifiers
     source          1..897
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             57..74
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             84..101
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             305..322
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(375..393)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      389..436
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      473..853
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(560..579)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     770..791
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 aattccctct gaatattttg tttactttaa gaaggagata taccatgggc agcagccatc
       61 atcatcatca tcacagcagc ggcctggttc cgcgtggtag tgagcacgtc ttcgcggtgg
      121 agagcatcga gaagaagcgg atccgcaagg gcagagtgga gtatctggtg aaatggagag
      181 gctggtcgcc caaatataac acgtgggaac cggaggagaa catcctggac cccaggctgc
      241 tgatcgcctt ccagaacagg gaacggcagg agcagtgatg acgaagcttg cggccgcact
      301 cgagcaccac caccaccacc actgagatcc ggctgctaac aaagcccgaa aggaagctga
      361 gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt
      421 cttgaggggt tttttgctga aaggaggaac tatatccgga ttggcgaatg ggacgcgccc
      481 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
      541 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
      601 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
      661 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
      721 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
      781 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
      841 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcg
//
