LOCUS       sequence-31318-e         906 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 906)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 906)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PRDM12
FEATURES             Location/Qualifiers
     source          1..906
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             58..75
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             85..102
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             642..659
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(712..730)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      726..773
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 gaattccctc tgaatatttt gtttacttta agaaggagat ataccatggg cagcagccat
       61 catcatcatc atcacagcag cggcctggtt ccgcgtggta gtaaaactgc ctttacagct
      121 gaggtcttgg cgcaatcctt ctcgggagaa gtgcagaagc ttagctctct cgtcttaccc
      181 gccgaggtaa taattgcaca aagttcaatc ccgggcgaag ggttgggaat tttttccaaa
      241 acctggatca aagctggcac ggagatgggt ccattcactg gccgggtgat agcgcctgaa
      301 cacgttgata tttgcaagaa caataacctt atgtgggaag tctttaatga ggacggtaca
      361 gtgagatact tcatcgatgc ctcgcaggaa gaccatcgaa gctggatgac ctatattaaa
      421 tgtgcacgta acgagcagga acaaaatctc gaagtagttc agattgggac gtctatcttt
      481 tacaaagcta tagagatgat cccgcccgat caagaattat tggtgtggta tggaaacagt
      541 cacaatacct tccttggcat tccgggtgtc ccaggcctgg aggaagatca gaagaaaaac
      601 aaacatgaag actgatgacg aagcttgcgg ccgcactcga gcaccaccac caccaccact
      661 gagatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc
      721 aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag
      781 gaggaactat atccggattg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc
      841 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
      901 tttcgc
//
