LOCUS       sequence-313193-        9021 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insert: PlexAtet-citrine-tCyc1. The fluorescent protein Citrine is 
            inducible by LexA-ER-B112 upon addition of estradiol, and 
            repressible by TetR. Backbone: pRG207 (addGene). Marker:Lys2..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9021)
  AUTHORS   Lormeau C, Rudolf F, Stelling J
  TITLE     A rationally engineered decoder of transient intracellular signals.
  JOURNAL   Nat Commun. 2021 Mar 25;12(1):1886. doi: 10.1038/s41467-021-22190-4.
  PUBMED    33767179
REFERENCE   2  (bases 1 to 9021)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-03-25"; volume: "12(1):1886. doi"; pages: " 
            10.1038/s41467-021-22190-4"
COMMENT     Sequence Label: pCL12-decoder
FEATURES             Location/Qualifiers
     source          1..9021
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(16..35)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(17..35)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(42..59)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(42..58)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(51..73)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     promoter        175..414
                     /gene="S. cerevisiae LYS2"
                     /label=LYS2 promoter
     CDS             415..4593
                     /codon_start=1
                     /gene="S. cerevisiae LYS2"
                     /product="alpha-aminoadipate reductase, required for lysine
                     biosynthesis"
                     /label=LYS2
                     /note="yeast auxotrophic marker"
                     /translation="MTNEKVWIEKLDNPTLSVLPHDFLRPQQEPYTKQATYSLQLPQLD
                     VPHDSFSNKYAVALSVWAALIYRVTGDDDIVLYIANNKILRFNIQPTWSFNELYSTINN
                     ELNKLNSIEANFSFDELAEKIQSCQDLERTPQLFRLAFLENQDFKLDEFKHHLVDFALN
                     LDTSNNAHVLNLIYNSLLYSNERVTIVADQFTQYLTAALSDPSNCITKISLITASSKDS
                     LPDPTKNLGWCDFVGCIHDIFQDNAEAFPERTCVVETPTLNSDKSRSFTYRDINRTSNI
                     VAHYLIKTGIKRGDVVMIYSSRGVDLMVCVMGVLKAGATFSVIDPAYPPARQTIYLGVA
                     KPRGLIVIRAAGQLDQLVEDYINDELEIVSRINSIAIQENGTIEGGKLDNGEDVLAPYD
                     HYKDTRTGVVVGPDSNPTLSFTSGSEGIPKGVLGRHFSLAYYFNWMSKRFNLTENDKFT
                     MLSGIAHDPIQRDMFTPLFLGAQLYVPTQDDIGTPGRLAEWMSKYGCTVTHLTPAMGQL
                     LTAQATTPFPKLHHAFFVGDILTKRDCLRLQTLAENCRIVNMYGTTETQRAVSYFEVKS
                     KNDDPNFLKKLKDVMPAGKGMLNVQLLVVNRNDRTQICGIGEIGEIYVRAGGLAEGYRG
                     LPELNKEKFVNNWFVEKDHWNYLDKDNGEPWRQFWLGPRDRLYRTGDLGRYLPNGDCEC
                     CGRADDQVKIRGFRIELGEIDTHISQHPLVRENITLVRKNADNEPTLITFMVPRFDKPD
                     DLSKFQSDVPKEVETDPIVKGLIGYHLLSKDIRTFLKKRLASYAMPSLIVVMDKLPLNP
                     NGKVDKPKLQFPTPKQLNLVAENTVSETDDSQFTNVEREVRDLWLSILPTKPASVSPDD
                     SFFDLGGHSILATKMIFTLKKKLQVDLPLGTIFKYPTIKAFAAEIDRIKSSGGSSQGEV
                     VENVTANYAEDAKKLVETLPSSYPSREYFVEPNSAEGKTTINVFVTGVTGFLGSYILAD
                     LLGRSPKNYSFKVFAHVRAKDEEAAFARLQKAGITYGTWNEKFASNIKVVLGDLSKSQF
                     GLSDEKWMDLANTVDIIIHNGALVHWVYPYAKLRDPNVISTINVMSLAAVGKPKFFDFV
                     SSTSTLDTEYYFNLSDKLVSEGKPGILESDDLMDSASGLTGGYGQSKWAAEYIIRRAGE
                     RGLRGCIVRPGYVTGASANGSSNTDDFLLRFLKGSVQLGKIPDIENSVNMVPVDHVARV
                     VVATSLNPPKENELAVAQVTGHPRILFKDYLYTLHDYGYDVEIESYSKWKKSLEASVID
                     RNEENALYPLLHMVLDNLPESTKAPELDDRNAVASLKKDTAWTGVDWSNGIGVTPEEVG
                     IYIAFLNKVGFLPPPTHNDKLPLPSIELTQAQISLVASGAGARGSSAAA"
     promoter        4990..5094
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5095..5163,5164..5955)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5313..5332)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6134..6722
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6623..6642
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6876..6893
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7267..7283
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7272..7294
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7291..7307
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7291..7307
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        7328..7346
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     terminator      complement(7509..7758)
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     7724..7742
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     CDS             complement(7799..8515)
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="MRSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
ORIGIN
        1 tgtttctcca attcgcccta tagtgagtcg tattacaatt cactggccgt cgttttacaa
       61 cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccgatc
      121 gagaggcatc gcacagtttt agcgaggaaa actcttcaat agttttgcca gcggaattgc
      181 acttgcaatt acataaaaaa ttccggcggt ttttcgcgtg tgactcaatg tcgaaatacc
      241 tgcctaatga acatgaacat cgcccaaatg tatttgaaga cccgctggga gaagttcaag
      301 atatataagt aacaagcagc caatagtata aaaaaaaatc tgagtttatt acctttcctg
      361 gaatttcagt gaaaaactgc taattataga gacatatcac agagttactc actaatgact
      421 aacgaaaagg tctggataga gaagttggat aatccaactc tttcagtgtt accacatgac
      481 tttttacgcc cacaacaaga accttatacg aaacaagcta catattcgtt acagctacct
      541 cagctcgatg tgcctcatga tagtttttct aacaaatacg ctgtcgcttt gagtgtatgg
      601 gctgcattga tatatagagt aactggtgac gatgatattg ttctttatat tgcgaataac
      661 aaaatcttaa gattcaatat tcaaccaacg tggtcattta atgagctgta ttctacaatt
      721 aacaatgagt tgaacaagct caattctatt gaggccaatt tttcctttga cgagctagct
      781 gaaaaaattc aaagttgcca agacttggaa aggacccctc agttgttccg tttggccttt
      841 ttggaaaacc aagatttcaa attagacgag ttcaagcatc atttagtgga ctttgctttg
      901 aatttggata ccagtaataa tgcgcatgtt ttgaacttaa tttataacag cttactgtat
      961 tcgaatgaaa gagtaaccat tgttgcggac caatttactc aatatttgac tgctgcgcta
     1021 agcgatccat ccaattgcat aactaaaatc tctctgatca ccgcatcatc caaggatagt
     1081 ttacctgatc caactaagaa cttgggctgg tgcgatttcg tggggtgtat tcacgacatt
     1141 ttccaggaca atgctgaagc cttcccagag agaacctgtg ttgtggagac tccaacacta
     1201 aattccgaca agtcccgttc tttcacttat cgcgacatca accgcacttc taacatagtt
     1261 gcccattatt tgattaaaac aggtatcaaa agaggtgatg tagtgatgat ctattcttct
     1321 aggggtgtgg atttgatggt atgtgtgatg ggtgtcttga aagctggcgc aaccttttca
     1381 gttatcgacc ctgcatatcc cccagccaga caaaccattt acttaggtgt tgctaaacca
     1441 cgtgggttga ttgttattag agctgctgga caattggatc aattagtaga agattacatc
     1501 aatgatgaat tggagattgt ttcaagaatc aattccatcg ctattcaaga aaatggtact
     1561 attgaaggtg gcaaattgga caatggcgag gatgttttgg ctccatacga tcactacaaa
     1621 gacaccagaa caggtgttgt agttggacca gattccaacc caaccctatc tttcacatct
     1681 ggttccgaag gtattcctaa gggtgttctt ggtagacatt tttccttggc ttattatttc
     1741 aattggatgt ccaaaaggtt caacttaaca gaaaatgata aattcacaat gctgagcggt
     1801 attgcacatg atccaattca aagagatatg tttacaccat tatttttagg tgcccaattg
     1861 tatgtcccta ctcaagatga tattggtaca ccgggccgtt tagcggaatg gatgagtaag
     1921 tatggttgca cagttaccca tttaacacct gccatgggtc aattacttac tgcccaagct
     1981 actacaccat tccctaagtt acatcatgcg ttctttgtgg gtgacatttt aacaaaacgt
     2041 gattgtctga ggttacaaac cttggcagaa aattgccgta ttgttaatat gtacggtact
     2101 actgaaacac agcgtgcagt ttcttatttc gaagttaaat caaaaaatga cgatccaaac
     2161 tttttgaaaa aattgaaaga tgtcatgcct gctggtaaag gtatgttgaa cgttcagcta
     2221 ttagttgtta acaggaacga ccgtactcaa atatgtggta ttggcgaaat aggtgagatt
     2281 tatgttcgtg caggtggttt ggccgaaggt tatagaggat taccagaatt gaataaagaa
     2341 aaatttgtga acaactggtt tgttgaaaaa gatcactgga attatttgga taaggataat
     2401 ggtgaacctt ggagacaatt ctggttaggt ccaagagata gattgtacag aacgggtgat
     2461 ttaggtcgtt atctaccaaa cggtgactgt gaatgttgcg gtagggctga tgatcaagtt
     2521 aaaattcgtg ggttcagaat cgaattagga gaaatagata cgcacatttc ccaacatcca
     2581 ttggtaagag aaaacattac tttagttcgc aaaaatgccg acaatgagcc aacattgatc
     2641 acatttatgg tcccaagatt tgacaagcca gatgacttgt ctaagttcca aagtgatgtt
     2701 ccaaaggagg ttgaaactga ccctatagtt aagggcttaa tcggttacca tcttttatcc
     2761 aaggacatca ggactttctt aaagaaaaga ttggctagct atgctatgcc ttccttgatt
     2821 gtggttatgg ataaactacc attgaatcca aatggtaaag ttgataagcc taaacttcaa
     2881 ttcccaactc ccaagcaatt aaatttggta gctgaaaata cagtttctga aactgacgac
     2941 tctcagttta ccaatgttga gcgcgaggtt agagacttat ggttaagtat attacctacc
     3001 aagccagcat ctgtatcacc agatgattcg tttttcgatt taggtggtca ttctatcttg
     3061 gctaccaaaa tgatttttac cttaaagaaa aagctgcaag ttgatttacc attgggcaca
     3121 attttcaagt atccaacgat aaaggccttt gccgcggaaa ttgacagaat taaatcatcg
     3181 ggtggatcat ctcaaggtga ggtcgtcgaa aatgtcactg caaattatgc ggaagacgcc
     3241 aagaaattgg ttgagacgct accaagttcg tacccctctc gtgaatattt tgttgaacct
     3301 aatagtgccg aaggaaaaac aacaattaat gtgtttgtta ctggtgtcac aggatttctg
     3361 ggctcctaca tccttgcaga tttgttagga cgttctccaa agaactacag tttcaaagtg
     3421 tttgcccacg tcagggccaa ggatgaagaa gctgcatttg caagattaca aaaggcaggt
     3481 atcacctatg gtacttggaa cgaaaaattt gcctcaaata ttaaagttgt attaggcgat
     3541 ttatctaaaa gccaatttgg tctttcagat gagaagtgga tggatttggc aaacacagtt
     3601 gatataatta tccataatgg tgcgttagtt cactgggttt atccatacgc caaattgaga
     3661 gatccaaatg ttatttcaac tatcaatgtt atgagcttag ccgccgtcgg caagccaaag
     3721 ttctttgact ttgtttcctc cacttctact cttgacactg aatactactt taatttgtca
     3781 gataaacttg ttagcgaagg gaagccaggc attttagaat cagacgattt aatggactct
     3841 gcaagcgggc tcactggtgg atatggtcag tccaaatggg ctgctgagta catcattaga
     3901 cgtgcaggtg aaaggggcct acgtgggtgt attgtcagac caggttacgt aacaggtgcc
     3961 tctgccaatg gttcttcaaa cacagatgat ttcttattga gatttttgaa aggttcagtc
     4021 caattaggta agattccaga catcgaaaat tccgtgaata tggttccagt agatcatgtt
     4081 gctcgtgttg ttgttgctac gtctttgaat cctcccaaag aaaatgaatt ggccgttgct
     4141 caagtaacgg gtcacccaag aatattattc aaagactact tgtatacttt acacgattat
     4201 ggttacgatg tcgaaatcga aagctattct aaatggaaga aatcattgga ggcgtctgtt
     4261 attgacagga atgaagaaaa tgcgttgtat cctttgctac acatggtctt agacaactta
     4321 cctgaaagta ccaaagctcc ggaactagac gataggaacg ccgtggcatc tttaaagaaa
     4381 gacaccgcat ggacaggtgt tgattggtct aatggaatag gtgttactcc agaagaggtt
     4441 ggtatatata ttgcattttt aaacaaggtt ggatttttac ctccaccaac tcataatgac
     4501 aaacttccac tgccaagtat agaactaact caagcgcaaa taagtctagt tgcttcaggt
     4561 gctggtgctc gtggaagctc cgcagcagct taaggttgag cattacgtat gatatgcata
     4621 tgtccatgta caataattaa atatgaatta ggagaaagac ttagcttctt ttcgggtgat
     4681 gtcacttaaa aactccgaga ataatatata ataagagaat aaaatattag ttattgaata
     4741 agaactgtaa atcggctggc gttagtctgc taatggcagc ttcatcttgg tttattgtag
     4801 catgaatcat atttgccttt ttttcctgta attcaatgat tcttgcttct atactatcct
     4861 caatgcaaaa ccttgtgatc ttcacaggcg cgccaattaa ttaatagcag ttcgcaaaat
     4921 cgttcgcttc gcaaatgttg ctaatgccgc gttctttcga cgtcaggtgg cacttttcgg
     4981 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     5041 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
     5101 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
     5161 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
     5221 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
     5281 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
     5341 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     5401 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     5461 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac catcggagga
     5521 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     5581 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
     5641 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     5701 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     5761 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     5821 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     5881 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     5941 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     6001 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     6061 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtaggttt aaacaaaaga
     6121 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     6181 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     6241 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     6301 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     6361 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     6421 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     6481 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     6541 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     6601 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     6661 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     6721 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     6781 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     6841 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     6901 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcctgc
     6961 aggatggcgc gcctgaaatt tacaaattgt tcgatttcac agcggacggt gttgcgtcaa
     7021 gggctgaaaa gacaatcaat tactacaaag gaaagcagtt gctttctcct atgggaagag
     7081 ctttctaagt ctgaagaagt aaacagttct ttgctatttc acacttcctg gttgatggtc
     7141 acttgctgcc tgaaatatat atatatgtat gacatatcta cttgttttct tttttgtgcc
     7201 tttgttacgt ctatattcat tgaaactgat tattcgattt tcttcttgct gaccgcttct
     7261 cagctgttgt gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca
     7321 agctcgaaat taaccctcac taaagggaac aaaagctggg tacaagtgac aatatatata
     7381 tatatatata ataacgacat cgttatctgt aaatacggcc gaagctgggt accaagcaga
     7441 cgctactaag gaaaacaaca acggaagcga tgatgagagc gacgatgaga gtgtaaactg
     7501 cgaaccttgc aaattaaagc cttcgagcgt cccaaaacct tctcaagcaa ggttttcagt
     7561 ataatgttac atgcgtacac gcgtttgtac agaaaaaaaa gaaaaatttg aaatataaat
     7621 aacgttctta atactaacat aactattaaa aaaaataaat agggacctag acttcaggtt
     7681 gtctaactcc ttccttttcg gttagagcgg atgtgggagg agggcgtgaa tgtaagcgtg
     7741 acataactaa ttacatgata tagacaaagg aaaaggggcc tgtctcgagt taggatcctt
     7801 tgtacaattc atccatacca tgggtaatac cagcagcagt aacaaattct aacaagacca
     7861 tgtggtctct cttttcgttt ggatctttgg ataacttaga ttgataggat aagtaatggt
     7921 tgtctggtaa caagactgga ccatcaccaa ttggagtatt ttgttgataa tggtcagcta
     7981 attgaacaga accatcttca atgttgtgtc taattttgaa gttaactttg ataccattct
     8041 tttgtttgtc agccatgatg taaacattgt gagagttata gttgtattcc aatttgtgac
     8101 ctaaaatgtt accatcttct ttaaaatcaa taccttttaa ttcgattcta ttaactaagg
     8161 tatcaccttc aaacttgact tcagctctgg tcttgtagtt accgtcatct ttgaaaaaaa
     8221 tagttctttc ttgaacataa ccttctggca tggcagactt gaaaaagtca tgttgtttca
     8281 tatgatctgg gtatctagca aaacacatca aaccataacc taaagtagtg actaaggttg
     8341 gccatggaac tggcaattta ccagtagtac aaataaattt taaggtcaat ttaccgtaag
     8401 tagcatcacc ttcaccttca ccggagacag aaaatttgtg accattaaca tcaccatcta
     8461 attcaaccaa aattgggaca acaccagtga ataattcttc acctttagat ctcataagct
     8521 tgatatcgaa ttcctgcagc ccgggggatc cactagtttt gtttgtttat gtgtgtttat
     8581 tcgaaactaa gttcttggtg ttttaaaact aaaaaaaaga ctaactataa aagtagaatt
     8641 taagaagttt aagaaataga tttacagaat tactctatca atgatagact tttatatcta
     8701 tcaatgatag ataggggaat aatttcaggg aactggtttc aaccttttct agaccctggg
     8761 tgtatataca gttatgctgt gagtatatac agcacctaga ccctgggtgt atatacagtt
     8821 atgctgtgag tatatacagc acctagaccc tgggtgtata tacagttatg ctgtgagtat
     8881 atacagcacc tagaccctgg gtgtatatac agttatgctg tgagtatata cagcacctag
     8941 ggagctccaa ttcgccctat aggcggccgc gaaaagaaat atagtcttca aggttatatt
     9001 atacaggttt atatattatt c
//
