LOCUS       sequence-313195-       13477 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Baculovirus protein expression of His6-Hs.NF1opt (1-2839) isoform 1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13477)
  AUTHORS   Sherekar M, Han SW, Ghirlando R, Messing S, Drew M, Rabara D, 
            Waybright T, Juneja P, O'Neill H, Stanley CB, Bhowmik D, Ramanathan 
            A, Subramaniam S, Nissley DV, Gillette W, McCormick F, Esposito D
  TITLE     Biochemical and structural analyses reveal that the tumor suppressor
            neurofibromin (NF1) forms a high-affinity dimer.
  JOURNAL   J Biol Chem. 2020 Jan 24;295(4):1105-1119. doi: 
            10.1074/jbc.RA119.010934. Epub 2019 Dec 13.
  PUBMED    31836666
REFERENCE   2  (bases 1 to 13477)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.RA119.010934"; journalName: "J Biol Chem"; date: 
            "2020-01-24- 24"; volume: "295"; issue: "4"; pages: "1105-1119"
COMMENT     Sequence Label: R702-X38-635: His6-Hs.NF1opt (1-2839) isoform 1
FEATURES             Location/Qualifiers
     source          1..13477
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..92
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     17..34
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             152..169
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     protein_bind    182..206
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      209..218
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(8738..8762)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    8920..9054
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8957..8976)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9011..9030
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  9083..9248
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      9432..9886
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9519..9538)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9728..9749
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9912..10016
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10017..10085,10086..10877)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10235..10254)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11048..11636
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11537..11556
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     mobile_element  11941..12165
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(12232..12765)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(12954..12959,12960..12976,12977..12982))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     promoter        complement(13345..13454)
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
ORIGIN
        1 atcatggaga taattaaaat gataaccatc tcgcaaataa ataagtattt tactgttttc
       61 gtaacagttt tgtaataaaa aaacctataa atattccgga ttattcatac cgtcccacca
      121 tcgggcgcgg atcaccatgg gatctggatc ccaccatcac catcaccata gatcctcgat
      181 cacaagtttg tacaaaaaag caggctgcgc caccatggcc gcccacagac ctgtggaatg
      241 ggtgcaggcc gtggtgtcca gattcgacga gcagctgccc atcaagaccg gacagcagaa
      301 tacacacacc aaggtgtcca ccgagcacaa caaagagtgc ctgatcaaca tcagcaagta
      361 caagttcagc ctggtgatca gcggcctgac caccatcctg aagaacgtga acaacatgcg
      421 gatcttcggc gaggccgccg agaagaacct gtacctgagc cagctgatca tcctggacac
      481 cctggaaaag tgcctcgctg gacagcccaa ggataccatg cggctggacg agacaatgct
      541 ggtgaagcag ctgctgcccg agatctgcca cttcctgcac acctgccggg agggaaatca
      601 gcacgccgcc gagctgagaa atagcgccag cggcgtgctg ttcagcctga gctgcaacaa
      661 cttcaacgcc gtgttcagcc ggatcagcac ccggctgcag gaactgaccg tctgcagcga
      721 ggacaacgtg gacgtgcacg acatcgagct gctgcagtac atcaacgtgg actgcgccaa
      781 gctgaagcgg ctgctgaaag agacagcctt caagttcaag gccctgaaga aggtggccca
      841 gctggccgtg atcaacagcc tggaaaaagc cttctggaac tgggtggaga actaccccga
      901 cgagttcacc aagctgtacc agatccccca gaccgacatg gccgagtgcg ccgagaagct
      961 gttcgacctg gtggacggct tcgccgagag caccaagaga aaggccgccg tgtggcccct
     1021 gcagatcatc ctgctgatcc tgtgccccga gatcatccag gacatctcca aggacgtggt
     1081 ggacgagaac aacatgaaca agaagctgtt tctggacagc ctgaggaagg ccctggccgg
     1141 acacggcggc tctagacagc tgaccgagag cgccgccatt gcctgcgtga agctgtgcaa
     1201 ggccagcacc tacatcaact gggaggacaa cagcgtgatc ttcctgctgg tgcagagcat
     1261 ggtggtggac ctgaagaacc tgctgttcaa ccccagcaag cccttcagca gaggcagcca
     1321 gcccgccgac gtggacctga tgatcgactg cctggtgtcc tgcttccgga tcagccccca
     1381 caacaaccag cacttcaaga tctgcctggc ccagaacagc cccagcacct tccactacgt
     1441 gctggtgaac tccctgcacc ggatcatcac caacagcgcc ctggactggt ggcccaagat
     1501 cgacgccgtg tactgccaca gcgtggagct gcggaacatg ttcggcgaga cactgcacaa
     1561 ggccgtgcag ggatgtggag cccaccccgc catcagaatg gcccccagcc tgaccttcaa
     1621 agaaaaggtg acctccctga agttcaaaga gaagcccacc gacctggaaa cccggtccta
     1681 caagtacctg ctgctgtcca tggtgaagct gatccacgcc gaccccaagc tgctgctgtg
     1741 caaccctagg aagcagggcc ctgagacaca gggcagcaca gccgagctga tcaccggcct
     1801 ggtgcagctg gtgccccaga gccacatgcc tgagatcgcc caggaagcca tggaagctct
     1861 gctcgtcctg caccagctcg actccattga tctgtggaac cccgacgccc ccgtggagac
     1921 attctgggag atcagcagcc agatgctgtt ctacatctgc aagaagctga ccagccatca
     1981 gatgctgtcc agcaccgaga tcctgaagtg gctgcgggag atcctgatct gccggaacaa
     2041 gttcctgctg aagaacaagc aggccgaccg gtccagctgc cactttctgc tgttttacgg
     2101 cgtgggctgc gacatcccca gcagcggcaa caccagccag atgagcatgg accacgagga
     2161 actgctgaga acccctggcg ccagcctgag aaagggcaag ggcaacagca gcatggacag
     2221 cgccgctggc tgtagcggca ccccccctat ctgtcggcag gcccagacaa aactggaagt
     2281 cgctctgtac atgttcctgt ggaatcccga taccgaggcc gtgctggtcg ccatgagctg
     2341 cttccggcac ctgtgcgagg aagccgacat cagatgcggc gtggatgagg tgtccgtgca
     2401 caacctgctg cccaactaca acaccttcat ggaattcgcc agcgtgagca acatgatgag
     2461 caccggcaga gccgccctgc agaaacgggt gatggccctg ctgcggagaa tcgagcaccc
     2521 caccgccgga aatacagagg cctgggagga tacccacgcc aagtgggagc aggccaccaa
     2581 gctgatcctg aactacccca aggccaagat ggaagatggc caggccgccg aaagcctgca
     2641 caagaccatc gtgaagcggc ggatgagcca cgtgtctggc ggcggaagca tcgacctgag
     2701 cgacaccgac tccctgcaag aatggatcaa catgaccggc tttctgtgtg ccctgggcgg
     2761 agtgtgcctg cagcagcgga gcaattccgg cctggccacc tacagccctc ctatgggccc
     2821 cgtgagcgag aggaagggca gcatgatcag cgtgatgtcc agcgagggca acgccgacac
     2881 ccccgtgagc aagttcatgg accggctgct gtccctgatg gtctgcaacc acgagaaagt
     2941 gggcctgcag atccggacca acgtgaagga cctggtcggc ctggaactga gccctgccct
     3001 gtaccccatg ctgttcaata agctgaagaa taccatctcc aagttcttcg acagccaggg
     3061 ccaggtgctg ctgaccgaca ccaacaccca gttcgtggag cagacaatcg ccatcatgaa
     3121 gaatctgctg gacaaccaca ccgagggcag ctctgaacac ctgggccagg ccagcatcga
     3181 gacaatgatg ctgaacctgg tccgctacgt gcgggtgctg ggcaacatgg tgcacgccat
     3241 ccagatcaag acaaagctgt gccagctggt cgaggtgatg atggccagac gggacgacct
     3301 gagcttctgc caggaaatga agttccggaa caagatggtg gagtacctga ccgactgggt
     3361 gatgggcacc agcaaccagg ccgctgacga cgacgtgaag tgcctgacca gggacctgga
     3421 ccaggcttct atggaagctg tcgtcagtct gctggccgga ctgcctctgc agcctgaaga
     3481 aggtgacggc gtcgagctga tggaagccaa gagccagctg ttcctgaagt acttcaccct
     3541 gttcatgaac ctgctgaacg actgcagcga ggtggaggat gagagcgccc agaccggcgg
     3601 cagaaagcgg ggcatgagca gaaggctggc ctccctgagg cactgtaccg tgctggccat
     3661 gtccaatctg ctgaacgcca acgtggatag cggcctgatg cacagcatcg gcctgggcta
     3721 ccacaaggac ctgcagaccc gggccacctt tatggaagtg ctgaccaaga tcctgcagca
     3781 gggcaccgag ttcgacaccc tggccgagac tgtgctggcc gacagattcg agagactggt
     3841 ggaactggtg accatgatgg gcgaccaggg cgagctgcct attgccatgg ccctggccaa
     3901 cgtggtgccc tgtagccagt gggatgagct ggccagggtc ctcgtcacac tgttcgacag
     3961 ccggcacctg ctgtaccagc tgctgtggaa tatgttcagc aaagaggtgg agctggccga
     4021 ctccatgcag accctgttca gaggcaactc cctggccagc aagatcatga ccttttgctt
     4081 caaggtgtac ggcgccacct acctgcagaa gctgctggac cccctgctga ggatcgtgat
     4141 caccagcagc gactggcagc acgtgagctt cgaggtggac cccaccagac tggaacccag
     4201 cgagagcctg gaagagaacc agcggaacct gctgcagatg accgagaagt tcttccacgc
     4261 catcatcagc agcagctccg agttcccccc ccagctgaga agcgtgtgcc actgcctgta
     4321 ccaggccacc tgccacagcc tgctgaacaa ggccaccgtg aaagagaaga aagaaaacaa
     4381 gaaaagcgtg gtgtcccagc ggttccccca gaatagcatc ggcgccgtgg gcagcgccat
     4441 gttcctgcgg ttcatcaacc ctgccatcgt gagcccttac gaggccggca tcctggataa
     4501 gaagccccct ccccggatcg agaggggcct gaagctgatg agcaagattc tgcagagcat
     4561 tgccaaccac gtgctgttta ccaaggaaga gcacatgcgg cccttcaacg acttcgtgaa
     4621 gagcaacttc gacgccgcca ggcggttctt cctggatatc gccagcgact gccctaccag
     4681 cgacgccgtg aaccacagcc tgtccttcat cagcgacggc aacgtcctgg ccctgcatag
     4741 actgctgtgg aacaaccagg aaaagatcgg ccagtacctg agcagcaacc gggaccacaa
     4801 ggctgtgggc aggcggccct tcgataagat ggccaccctg ctggcctatc tgggccctcc
     4861 cgagcacaag cctgtggccg acacccattg gtctagcctg aacctgacca gcagcaagtt
     4921 cgaggaattc atgacccggc accaggtgca cgagaaagaa gagtttaaag ccctgaaaac
     4981 cctgagcatc ttctaccagg ccggcacctc caaggccggc aaccccatct tctactacgt
     5041 ggccagaagg ttcaagacag gccagatcaa cggcgacctg ctgatctacc acgtgctgct
     5101 gacactgaag ccctactacg ccaagcccta cgagatcgtg gtggatctga cccacaccgg
     5161 ccccagcaac cggttcaaga ccgacttcct gagcaagtgg ttcgtggtgt tccccggctt
     5221 cgcctacgat aacgtgagcg ccgtgtacat ctacaactgc aacagctggg tgcgggagta
     5281 caccaagtac cacgagcgcc tgctgaccgg cctgaagggc tccaagcggc tggtgttcat
     5341 cgactgcccc ggcaagctgg ccgagcacat cgagcacgag cagcagaaac tgcctgccgc
     5401 caccctggcc ctggaagagg acctgaaggt gttccacaac gccctgaagc tggcccacaa
     5461 ggatacaaag gtgtccatca aagtgggcag caccgccgtg caggtgacca gcgccgagag
     5521 gacaaaggtg ctgggccaga gcgtgttcct gaacgacatc tactacgcct ccgagatcga
     5581 ggaaatctgc ctggtggatg agaaccagtt caccctgact atcgccaacc agggcacccc
     5641 tctgaccttc atgcaccagg aatgcgaggc catcgtccag tccatcatcc acatccggac
     5701 cagatgggag ctgtcccagc ccgacagcat cccccagcac accaagatcc ggcccaagga
     5761 tgtgcccggc accctgctga atatcgctct gctgaatctg ggcagcagcg acccttctct
     5821 gcggagcgcc gcctacaatc tgctgtgcgc cctgacctgc accttcaacc tgaagatcga
     5881 gggccagctg ctggaaacca gcggcctgtg catccccgcc aacaacaccc tgtttatcgt
     5941 gagcatcagc aagaccctgg ccgccaacga gcctcacctg accctggaat ttctggaaga
     6001 gtgcatcagc ggcttcagca agagcagcat cgagctgaag cacctgtgcc tggaatacat
     6061 gaccccctgg ctgtctaatc tggtccggtt ctgcaagcac aacgacgacg ccaagcggca
     6121 gagagtgacc gccattctgg acaagctgat cacaatgacc atcaacgaga agcagatgta
     6181 ccccagcatc caggccaaga tctggggcag cctgggccag atcaccgatc tgctggatgt
     6241 ggtgctggac agcttcatca agaccagcgc caccggcgga ctgggaagca tcaaggccga
     6301 ggtgatggcc gatacagccg tggccctggc tagcggcaac gtgaagctgg tgtccagcaa
     6361 ggtgatcggc cggatgtgca agatcatcga caagacctgc ctgagcccca cccctaccct
     6421 ggaacagcac ctgatgtggg acgatatcgc catcctggcc cggtacatgc tgatgctgtc
     6481 cttcaacaat agcctggacg tggccgccca tctgccctac ctgttccacg tggtgacctt
     6541 tctggtggcc accggccctc tgagcctgag agccagcacc cacggcctgg tgattaacat
     6601 catccactcc ctgtgcacct gctcccagct gcacttcagc gaggaaacca agcaggtgct
     6661 gagactgagc ctgaccgagt tctccctgcc caagttctac ctgctgtttg gcatcagcaa
     6721 agtgaagtct gccgccgtga tcgccttcag gtccagctac agggacagat ctttctcccc
     6781 cggaagctac gagagagaga cattcgccct gacaagcctg gaaaccgtga cagaggccct
     6841 gctggaaatc atggaagcct gcatgagaga catccccacc tgcaagtggc tcgaccagtg
     6901 gaccgaactg gcccagaggt tcgccttcca gtacaacccc agcctgcagc ctagagccct
     6961 ggtggtgttc ggctgcatct ccaagagagt gagccacggc cagattaagc agatcatcag
     7021 gatcctgtcc aaggccctgg aaagctgtct gaagggcccc gacacctaca actcccaggt
     7081 gctgatcgag gccacagtga tcgccctgac caaactgcag ccactgctga ataaggacag
     7141 ccccctgcac aaagccctgt tctgggtggc cgtggccgtg ctgcagctgg acgaggtgaa
     7201 cctgtacagc gccgggaccg ctctgctgga acagaatctg cacaccctgg actccctgag
     7261 aatcttcaac gacaagagcc ccgaggaagt gttcatggcc atccggaacc ccctggaatg
     7321 gcactgcaag cagatggacc acttcgtggg cctgaacttc aacagcaact tcaacttcgc
     7381 cctggtcgga cacctgctga agggctacag acaccccagc ccagccatcg tggccaggac
     7441 cgtgcggatc ctgcataccc tgctgaccct ggtgaacaag caccggaact gcgacaagtt
     7501 tgaggtgaac acccagagcg tggcctacct ggctgccctg ctgacagtga gcgaggaagt
     7561 gcggagccgg tgcagcctga agcacaggaa gagcctgctc ctcactgata tcagcatgga
     7621 aaacgtgccc atggacacct accccatcca ccacggcgac cccagctaca ggaccctgaa
     7681 agaaacccag ccctggtcct cccctaaggg cagcgagggc tatctggccg ccacctatcc
     7741 taccgtcggc cagacaagcc ctagggcccg caagtccatg agcctggaca tgggccagcc
     7801 tagccaggcc aacaccaaga aactgctggg cacccggaag agcttcgacc acctgatcag
     7861 cgacaccaag gcccccaaga ggcaggaaat ggaaagcggc atcaccaccc cccccaagat
     7921 gcggagagtg gccgagacag actacgagat ggaaacccag cggatcagca gctcccagca
     7981 gcaccctcac ctgcggaaag tgtccgtgtc cgagagcaat gtgctgctgg acgaagaggt
     8041 gctgaccgat cctaagatcc aggccctgct gctgactgtc ctggccaccc tggtgaagta
     8101 caccaccgac gagttcgatc agcggatcct gtacgagtac ctggccgagg ccagcgtggt
     8161 gtttcccaag gtgttccctg tggtgcacaa tctgctggat agcaagatca acaccctgct
     8221 gtctctgtgc caggacccca acctgctgaa tcccatccac ggcatcgtgc agagtgtggt
     8281 gtaccacgaa gagagccccc ctcagtacca gaccagctac ctgcagtcct tcggcttcaa
     8341 cggcctgtgg cggtttgccg gccctttcag caagcagacc cagatccccg actacgccga
     8401 actgatcgtg aagttcctgg acgccctgat cgatacctac ctgcccggca tcgacgagga
     8461 aacctccgag gaatctctgc tgacccccac ctccccttat cctccagccc tgcagtccca
     8521 gctgtccatc accgccaacc tgaacctgtc caactccatg accagcctgg ccacatctca
     8581 gcacagccct ggcatcgaca aagaaaacgt ggagctgtct cccaccaccg gccactgcaa
     8641 ctccggcagg accagacacg gaagcgccag ccaggtgcag aagcagagaa gcgccggcag
     8701 cttcaagcgg aacagcatca agaaaatcgt gtgatgaacc cagctttctt gtacaaagtg
     8761 gtgataatta attaagatcg agctagctag aagcttgtcg agaagtacta gaggatcata
     8821 atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc
     8881 ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat
     8941 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
     9001 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatctgatca
     9061 ctgcttgagc ctaggagatc cgaaccagat aagtgaaatc tagttccaaa ctattttgtc
     9121 atttttaatt ttcgtattag cttacgacgc tacacccagt tcccatctat tttgtcactc
     9181 ttccctaaat aatccttaaa aactccattt ccacccctcc cagttcccaa ctattttgtc
     9241 cgcccacagc ggggcatttt tcttcctgtt atgtttttaa tcaaacatcc tgccaactcc
     9301 atgtgacaaa ccgtcatctt cggctacttt ttctctgtca cagaatgaaa atttttctgt
     9361 catctcttcg ttattaatgt ttgtaattga ctgaatatca acgcttattt gcagcctgaa
     9421 tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
     9481 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
     9541 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
     9601 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
     9661 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
     9721 cttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct
     9781 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
     9841 caaaaattta acgcgaattt taacaaaata ttaacgctta caatttaggt ggcacttttc
     9901 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     9961 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
    10021 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
    10081 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
    10141 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
    10201 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
    10261 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
    10321 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
    10381 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
    10441 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
    10501 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
    10561 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
    10621 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
    10681 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
    10741 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
    10801 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
    10861 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
    10921 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
    10981 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
    11041 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
    11101 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
    11161 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
    11221 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
    11281 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
    11341 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
    11401 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
    11461 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
    11521 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
    11581 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
    11641 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
    11701 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
    11761 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
    11821 gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atagaccagc
    11881 cgcgtaacct ggcaaaatcg gttacggttg agtaataaat ggatgccctg cgtaagcggg
    11941 tgtgggcgga caataaagtc ttaaactgaa caaaatagat ctaaactatg acaataaagt
    12001 cttaaactag acagaatagt tgtaaactga aatcagtcca gttatgctgt gaaaaagcat
    12061 actggacttt tgttatggct aaagcaaact cttcattttc tgaagtgcaa attgcccgtc
    12121 gtattaaaga ggggcgtggc caagggcatg gtaaagacta tattcgcggc gttgtgacaa
    12181 tttaccgaac aactccgcgg ccgggaagcc gatctcggct tgaacgaatt gttaggtggc
    12241 ggtacttggg tcgatatcaa agtgcatcac ttcttcccgt atgcccaact ttgtatagag
    12301 agccactgcg ggatcgtcac cgtaatctgc ttgcacgtag atcacataag caccaagcgc
    12361 gttggcctca tgcttgagga gattgatgag cgcggtggca atgccctgcc tccggtgctc
    12421 gccggagact gcgagatcat agatatagat ctcactacgc ggctgctcaa acttgggcag
    12481 aacgtaagcc gcgagagcgc caacaaccgc ttcttggtcg aaggcagcaa gcgcgatgaa
    12541 tgtcttacta cggagcaagt tcccgaggta atcggagtcc ggctgatgtt gggagtaggt
    12601 ggctacgtct ccgaactcac gaccgaaaag atcaagagca gcccgcatgg atttgacttg
    12661 gtcagggccg agcctacatg tgcgaatgat gcccatactt gagccaccta actttgtttt
    12721 agggcgactg ccctgctgcg taacatcgtt gctgctgcgt aacatcgttg ctgctccata
    12781 acatcaaaca tcgacccacg gcgtaacgcg cttgctgctt ggatgcccga ggcatagact
    12841 gtacaaaaaa acagtcataa caagccatga aaaccgccac tgcgccgtta ccaccgctgc
    12901 gttcggtcaa ggttctggac cagttgcgtg agcgcatacg ctacttgcat tacagtttac
    12961 gaaccgaaca ggcttatgtc aactgggttc gtgccttcat ccgtttccac ggtgtgcgtc
    13021 acccggcaac cttgggcagc agcgaagtcg aggcatttct gtcctggctg gcgaacgagc
    13081 gcaaggtttc ggtctccacg catcgtcagg cattggcggc cttgctgttc ttctacggca
    13141 aggtgctgtg cacggatctg cccttgcttc aggagatcgg tagacctcgg ccgtcgcggc
    13201 gcttgccggt ggtgctgacc ccggatgaag tggttcgcat cctcggtttt ctggaaggcg
    13261 agcatcgttt gttcgcccag gactctagct atagttctag tggttggcta ctcgagatcc
    13321 cgggtgatca agtcttcgtc gagtgattgt aaataaaatg taatttacag tatagtattt
    13381 taattaatat acaaatgatt tgataataat tcttatttaa ctataatata ttgtgttggg
    13441 ttgaattaaa ggtccgtata ctccggaata ttaatag
//
