LOCUS       sequence-313197-        9192 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insert: Pfus1tet-citrine-tCyc1. The fluorescent protein Citrine is 
            inducible by alpha-factor and repressible by TetR. Backbone: pRG207 
            (addGene). Marker:Lys2..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9192)
  AUTHORS   Lormeau C, Rudolf F, Stelling J
  TITLE     A rationally engineered decoder of transient intracellular signals.
  JOURNAL   Nat Commun. 2021 Mar 25;12(1):1886. doi: 10.1038/s41467-021-22190-4.
  PUBMED    33767179
REFERENCE   2  (bases 1 to 9192)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-03-25"; volume: "12(1):1886. doi"; pages: " 
            10.1038/s41467-021-22190-4"
COMMENT     Sequence Label: pCL27-decoder
FEATURES             Location/Qualifiers
     source          1..9192
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     160..182
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     174..191
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     175..191
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     198..217
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        198..216
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             910..1626
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="MRSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     terminator      1667..1916
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(1683..1701)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     promoter        complement(2079..2097)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2118..2134)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2118..2134)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2131..2153)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2142..2158
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(2532..2549)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2703..3291)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2783..2802)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3470..4261,4262..4330))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4093..4112
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4331..4435)
                     /gene="bla"
                     /label=AmpR promoter
     CDS             complement(4832..9010)
                     /codon_start=1
                     /gene="S. cerevisiae LYS2"
                     /product="alpha-aminoadipate reductase, required for lysine
                     biosynthesis"
                     /label=LYS2
                     /note="yeast auxotrophic marker"
                     /translation="MTNEKVWIEKLDNPTLSVLPHDFLRPQQEPYTKQATYSLQLPQLD
                     VPHDSFSNKYAVALSVWAALIYRVTGDDDIVLYIANNKILRFNIQPTWSFNELYSTINN
                     ELNKLNSIEANFSFDELAEKIQSCQDLERTPQLFRLAFLENQDFKLDEFKHHLVDFALN
                     LDTSNNAHVLNLIYNSLLYSNERVTIVADQFTQYLTAALSDPSNCITKISLITASSKDS
                     LPDPTKNLGWCDFVGCIHDIFQDNAEAFPERTCVVETPTLNSDKSRSFTYRDINRTSNI
                     VAHYLIKTGIKRGDVVMIYSSRGVDLMVCVMGVLKAGATFSVIDPAYPPARQTIYLGVA
                     KPRGLIVIRAAGQLDQLVEDYINDELEIVSRINSIAIQENGTIEGGKLDNGEDVLAPYD
                     HYKDTRTGVVVGPDSNPTLSFTSGSEGIPKGVLGRHFSLAYYFNWMSKRFNLTENDKFT
                     MLSGIAHDPIQRDMFTPLFLGAQLYVPTQDDIGTPGRLAEWMSKYGCTVTHLTPAMGQL
                     LTAQATTPFPKLHHAFFVGDILTKRDCLRLQTLAENCRIVNMYGTTETQRAVSYFEVKS
                     KNDDPNFLKKLKDVMPAGKGMLNVQLLVVNRNDRTQICGIGEIGEIYVRAGGLAEGYRG
                     LPELNKEKFVNNWFVEKDHWNYLDKDNGEPWRQFWLGPRDRLYRTGDLGRYLPNGDCEC
                     CGRADDQVKIRGFRIELGEIDTHISQHPLVRENITLVRKNADNEPTLITFMVPRFDKPD
                     DLSKFQSDVPKEVETDPIVKGLIGYHLLSKDIRTFLKKRLASYAMPSLIVVMDKLPLNP
                     NGKVDKPKLQFPTPKQLNLVAENTVSETDDSQFTNVEREVRDLWLSILPTKPASVSPDD
                     SFFDLGGHSILATKMIFTLKKKLQVDLPLGTIFKYPTIKAFAAEIDRIKSSGGSSQGEV
                     VENVTANYAEDAKKLVETLPSSYPSREYFVEPNSAEGKTTINVFVTGVTGFLGSYILAD
                     LLGRSPKNYSFKVFAHVRAKDEEAAFARLQKAGITYGTWNEKFASNIKVVLGDLSKSQF
                     GLSDEKWMDLANTVDIIIHNGALVHWVYPYAKLRDPNVISTINVMSLAAVGKPKFFDFV
                     SSTSTLDTEYYFNLSDKLVSEGKPGILESDDLMDSASGLTGGYGQSKWAAEYIIRRAGE
                     RGLRGCIVRPGYVTGASANGSSNTDDFLLRFLKGSVQLGKIPDIENSVNMVPVDHVARV
                     VVATSLNPPKENELAVAQVTGHPRILFKDYLYTLHDYGYDVEIESYSKWKKSLEASVID
                     RNEENALYPLLHMVLDNLPESTKAPELDDRNAVASLKKDTAWTGVDWSNGIGVTPEEVG
                     IYIAFLNKVGFLPPPTHNDKLPLPSIELTQAQISLVASGAGARGSSAAA"
     promoter        complement(join(9011..9192,1..58))
                     /gene="S. cerevisiae LYS2"
                     /label=LYS2 promoter
ORIGIN
        1 gacattgagt cacacgcgaa aaaccgccgg aattttttat gtaattgcaa gtgcaattcc
       61 gctggcaaaa ctattgaaga gttttcctcg ctaaaactgt gcgatgcctc tcgatcggat
      121 gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa
      181 cgacggccag tgaattgtaa tacgactcac tatagggcga attggagaaa cagaataata
      241 tataaacctg tataatataa ccttgaagac tatatttctt ttcgcggccg cctatagggc
      301 gaattggagc tccctaggac gatgattcag ttcgccttct atcctttgtt tacgtatttg
      361 tttatatata taactttatt tttttttatt aattgggctg caagacaatt ttgttgtcag
      421 tgatgcctca atccttcttt tgcttccata tttaccatgt ggaccctttc aaaacagagt
      481 tgtatctctg caggatgccc tttttgacgt attgaatggc ataattgcac tgtcactttt
      541 cgcgctgtct cattttggtg cgatgatgaa acaaacatga aacgtctgta atttgaaaca
      601 aataacgtaa ttctcgggat tggttttatt taaatgacaa tgtaagagtg gctttgtaag
      661 gtatgtgttg ctctagaaaa ggttgaaacc agttccctga aattattccc ctatctatca
      721 ttgatagata taaaagtcta tcattgatag agtaattctg taaatctatt tcttaaactt
      781 cttaaattct acttttatag ttagtctttt ttttagtttt aaaacaccaa gaacttagtt
      841 tcgaataaac acacataaac aaacaaaact agtggatccc ccgggctgca ggaattcgat
      901 atcaagctta tgagatctaa aggtgaagaa ttattcactg gtgttgtccc aattttggtt
      961 gaattagatg gtgatgttaa tggtcacaaa ttttctgtct ccggtgaagg tgaaggtgat
     1021 gctacttacg gtaaattgac cttaaaattt atttgtacta ctggtaaatt gccagttcca
     1081 tggccaacct tagtcactac tttaggttat ggtttgatgt gttttgctag atacccagat
     1141 catatgaaac aacatgactt tttcaagtct gccatgccag aaggttatgt tcaagaaaga
     1201 actatttttt tcaaagatga cggtaactac aagaccagag ctgaagtcaa gtttgaaggt
     1261 gataccttag ttaatagaat cgaattaaaa ggtattgatt ttaaagaaga tggtaacatt
     1321 ttaggtcaca aattggaata caactataac tctcacaatg tttacatcat ggctgacaaa
     1381 caaaagaatg gtatcaaagt taacttcaaa attagacaca acattgaaga tggttctgtt
     1441 caattagctg accattatca acaaaatact ccaattggtg atggtccagt cttgttacca
     1501 gacaaccatt acttatccta tcaatctaag ttatccaaag atccaaacga aaagagagac
     1561 cacatggtct tgttagaatt tgttactgct gctggtatta cccatggtat ggatgaattg
     1621 tacaaaggat cctaactcga gacaggcccc ttttcctttg tctatatcat gtaattagtt
     1681 atgtcacgct tacattcacg ccctcctccc acatccgctc taaccgaaaa ggaaggagtt
     1741 agacaacctg aagtctaggt ccctatttat tttttttaat agttatgtta gtattaagaa
     1801 cgttatttat atttcaaatt tttctttttt ttctgtacaa acgcgtgtac gcatgtaaca
     1861 ttatactgaa aaccttgctt gagaaggttt tgggacgctc gaaggcttta atttgcaagg
     1921 ttcgcagttt acactctcat cgtcgctctc atcatcgctt ccgttgttgt tttccttagt
     1981 agcgtctgct tggtacccag cttcggccgt atttacagat aacgatgtcg ttattatata
     2041 tatatatata tattgtcact tgtacccagc ttttgttccc tttagtgagg gttaatttcg
     2101 agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaaca
     2161 gctgagaagc ggtcagcaag aagaaaatcg aataatcagt ttcaatgaat atagacgtaa
     2221 caaaggcaca aaaaagaaaa caagtagata tgtcatacat atatatatat ttcaggcagc
     2281 aagtgaccat caaccaggaa gtgtgaaata gcaaagaact gtttacttct tcagacttag
     2341 aaagctcttc ccataggaga aagcaactgc tttcctttgt agtaattgat tgtcttttca
     2401 gcccttgacg caacaccgtc cgctgtgaaa tcgaacaatt tgtaaatttc aggcgcgcca
     2461 tcctgcaggc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
     2521 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     2581 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     2641 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     2701 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     2761 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     2821 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     2881 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     2941 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     3001 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     3061 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     3121 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
     3181 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     3241 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     3301 ttgatctttt gtttaaacct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     3361 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     3421 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     3481 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     3541 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     3601 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     3661 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     3721 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     3781 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     3841 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     3901 tcggtcctcc gatggttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     3961 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     4021 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     4081 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     4141 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     4201 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     4261 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     4321 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     4381 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     4441 ttccccgaaa agtgccacct gacgtcgaaa gaacgcggca ttagcaacat ttgcgaagcg
     4501 aacgattttg cgaactgcta ttaattaatt ggcgcgcctg tgaagatcac aaggttttgc
     4561 attgaggata gtatagaagc aagaatcatt gaattacagg aaaaaaaggc aaatatgatt
     4621 catgctacaa taaaccaaga tgaagctgcc attagcagac taacgccagc cgatttacag
     4681 ttcttattca ataactaata ttttattctc ttattatata ttattctcgg agtttttaag
     4741 tgacatcacc cgaaaagaag ctaagtcttt ctcctaattc atatttaatt attgtacatg
     4801 gacatatgca tatcatacgt aatgctcaac cttaagctgc tgcggagctt ccacgagcac
     4861 cagcacctga agcaactaga cttatttgcg cttgagttag ttctatactt ggcagtggaa
     4921 gtttgtcatt atgagttggt ggaggtaaaa atccaacctt gtttaaaaat gcaatatata
     4981 taccaacctc ttctggagta acacctattc cattagacca atcaacacct gtccatgcgg
     5041 tgtctttctt taaagatgcc acggcgttcc tatcgtctag ttccggagct ttggtacttt
     5101 caggtaagtt gtctaagacc atgtgtagca aaggatacaa cgcattttct tcattcctgt
     5161 caataacaga cgcctccaat gatttcttcc atttagaata gctttcgatt tcgacatcgt
     5221 aaccataatc gtgtaaagta tacaagtagt ctttgaataa tattcttggg tgacccgtta
     5281 cttgagcaac ggccaattca ttttctttgg gaggattcaa agacgtagca acaacaacac
     5341 gagcaacatg atctactgga accatattca cggaattttc gatgtctgga atcttaccta
     5401 attggactga acctttcaaa aatctcaata agaaatcatc tgtgtttgaa gaaccattgg
     5461 cagaggcacc tgttacgtaa cctggtctga caatacaccc acgtaggccc ctttcacctg
     5521 cacgtctaat gatgtactca gcagcccatt tggactgacc atatccacca gtgagcccgc
     5581 ttgcagagtc cattaaatcg tctgattcta aaatgcctgg cttcccttcg ctaacaagtt
     5641 tatctgacaa attaaagtag tattcagtgt caagagtaga agtggaggaa acaaagtcaa
     5701 agaactttgg cttgccgacg gcggctaagc tcataacatt gatagttgaa ataacatttg
     5761 gatctctcaa tttggcgtat ggataaaccc agtgaactaa cgcaccatta tggataatta
     5821 tatcaactgt gtttgccaaa tccatccact tctcatctga aagaccaaat tggcttttag
     5881 ataaatcgcc taatacaact ttaatatttg aggcaaattt ttcgttccaa gtaccatagg
     5941 tgatacctgc cttttgtaat cttgcaaatg cagcttcttc atccttggcc ctgacgtggg
     6001 caaacacttt gaaactgtag ttctttggag aacgtcctaa caaatctgca aggatgtagg
     6061 agcccagaaa tcctgtgaca ccagtaacaa acacattaat tgttgttttt ccttcggcac
     6121 tattaggttc aacaaaatat tcacgagagg ggtacgaact tggtagcgtc tcaaccaatt
     6181 tcttggcgtc ttccgcataa tttgcagtga cattttcgac gacctcacct tgagatgatc
     6241 cacccgatga tttaattctg tcaatttccg cggcaaaggc ctttatcgtt ggatacttga
     6301 aaattgtgcc caatggtaaa tcaacttgca gctttttctt taaggtaaaa atcattttgg
     6361 tagccaagat agaatgacca cctaaatcga aaaacgaatc atctggtgat acagatgctg
     6421 gcttggtagg taatatactt aaccataagt ctctaacctc gcgctcaaca ttggtaaact
     6481 gagagtcgtc agtttcagaa actgtatttt cagctaccaa atttaattgc ttgggagttg
     6541 ggaattgaag tttaggctta tcaactttac catttggatt caatggtagt ttatccataa
     6601 ccacaatcaa ggaaggcata gcatagctag ccaatctttt ctttaagaaa gtcctgatgt
     6661 ccttggataa aagatggtaa ccgattaagc ccttaactat agggtcagtt tcaacctcct
     6721 ttggaacatc actttggaac ttagacaagt catctggctt gtcaaatctt gggaccataa
     6781 atgtgatcaa tgttggctca ttgtcggcat ttttgcgaac taaagtaatg ttttctctta
     6841 ccaatggatg ttgggaaatg tgcgtatcta tttctcctaa ttcgattctg aacccacgaa
     6901 ttttaacttg atcatcagcc ctaccgcaac attcacagtc accgtttggt agataacgac
     6961 ctaaatcacc cgttctgtac aatctatctc ttggacctaa ccagaattgt ctccaaggtt
     7021 caccattatc cttatccaaa taattccagt gatctttttc aacaaaccag ttgttcacaa
     7081 atttttcttt attcaattct ggtaatcctc tataaccttc ggccaaacca cctgcacgaa
     7141 cataaatctc acctatttcg ccaataccac atatttgagt acggtcgttc ctgttaacaa
     7201 ctaatagctg aacgttcaac atacctttac cagcaggcat gacatctttc aattttttca
     7261 aaaagtttgg atcgtcattt tttgatttaa cttcgaaata agaaactgca cgctgtgttt
     7321 cagtagtacc gtacatatta acaatacggc aattttctgc caaggtttgt aacctcagac
     7381 aatcacgttt tgttaaaatg tcacccacaa agaacgcatg atgtaactta gggaatggtg
     7441 tagtagcttg ggcagtaagt aattgaccca tggcaggtgt taaatgggta actgtgcaac
     7501 catacttact catccattcc gctaaacggc ccggtgtacc aatatcatct tgagtaggga
     7561 catacaattg ggcacctaaa aataatggtg taaacatatc tctttgaatt ggatcatgtg
     7621 caataccgct cagcattgtg aatttatcat tttctgttaa gttgaacctt ttggacatcc
     7681 aattgaaata ataagccaag gaaaaatgtc taccaagaac acccttagga ataccttcgg
     7741 aaccagatgt gaaagatagg gttgggttgg aatctggtcc aactacaaca cctgttctgg
     7801 tgtctttgta gtgatcgtat ggagccaaaa catcctcgcc attgtccaat ttgccacctt
     7861 caatagtacc attttcttga atagcgatgg aattgattct tgaaacaatc tccaattcat
     7921 cattgatgta atcttctact aattgatcca attgtccagc agctctaata acaatcaacc
     7981 cacgtggttt agcaacacct aagtaaatgg tttgtctggc tgggggatat gcagggtcga
     8041 taactgaaaa ggttgcgcca gctttcaaga cacccatcac acataccatc aaatccacac
     8101 ccctagaaga atagatcatc actacatcac ctcttttgat acctgtttta atcaaataat
     8161 gggcaactat gttagaagtg cggttgatgt cgcgataagt gaaagaacgg gacttgtcgg
     8221 aatttagtgt tggagtctcc acaacacagg ttctctctgg gaaggcttca gcattgtcct
     8281 ggaaaatgtc gtgaatacac cccacgaaat cgcaccagcc caagttctta gttggatcag
     8341 gtaaactatc cttggatgat gcggtgatca gagagatttt agttatgcaa ttggatggat
     8401 cgcttagcgc agcagtcaaa tattgagtaa attggtccgc aacaatggtt actctttcat
     8461 tcgaatacag taagctgtta taaattaagt tcaaaacatg cgcattatta ctggtatcca
     8521 aattcaaagc aaagtccact aaatgatgct tgaactcgtc taatttgaaa tcttggtttt
     8581 ccaaaaaggc caaacggaac aactgagggg tcctttccaa gtcttggcaa ctttgaattt
     8641 tttcagctag ctcgtcaaag gaaaaattgg cctcaataga attgagcttg ttcaactcat
     8701 tgttaattgt agaatacagc tcattaaatg accacgttgg ttgaatattg aatcttaaga
     8761 ttttgttatt cgcaatataa agaacaatat catcgtcacc agttactcta tatatcaatg
     8821 cagcccatac actcaaagcg acagcgtatt tgttagaaaa actatcatga ggcacatcga
     8881 gctgaggtag ctgtaacgaa tatgtagctt gtttcgtata aggttcttgt tgtgggcgta
     8941 aaaagtcatg tggtaacact gaaagagttg gattatccaa cttctctatc cagacctttt
     9001 cgttagtcat tagtgagtaa ctctgtgata tgtctctata attagcagtt tttcactgaa
     9061 attccaggaa aggtaataaa ctcagatttt tttttatact attggctgct tgttacttat
     9121 atatcttgaa cttctcccag cgggtcttca aatacatttg ggcgatgttc atgttcatta
     9181 ggcaggtatt tc
//
