LOCUS       sequence-31321-e         905 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 905)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 905)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: CBX7
FEATURES             Location/Qualifiers
     source          1..905
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             47..64
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             77..97
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             292..309
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(362..380)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      376..423
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      460..888
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(547..566)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     757..778
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 attccctctg aatattttgt ttactttaag aaggagatat accatgcatc atcatcatca
       61 tcacagcagc ggcagagaaa acttgtattt ccagggcgag caggtgttcg ccgtggagag
      121 catccggaag aagcgcgtgc ggaagggtaa agtcgagtat ctggtgaagt ggaaaggatg
      181 gcccccaaag tacagcacgt gggagccaga agagcacatc ttggaccccc gcctcgtcat
      241 ggcctacgag gagaaggagg agtgatgacg aagcttgcgg ccgcactcga gcaccaccac
      301 caccaccact gagatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc
      361 accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt
      421 ttgctgaaag gaggaactat atccggattg gcgaatggga cgcgccctgt agcggcgcat
      481 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
      541 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
      601 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
      661 ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt
      721 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
      781 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg
      841 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaatatt
      901 aacgc
//
