LOCUS       sequence-313219-       10240 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10240)
  AUTHORS   Edie S, Zaghloul NA, Leitch CC, Klinedinst DK, Lebron J, Thole JF, 
            McCallion AS, Katsanis N, Reeves RH
  TITLE     Survey of Human Chromosome 21 Gene Expression Effects on Early 
            Development in Danio rerio.
  JOURNAL   G3 (Bethesda). 2018 Jul 2;8(7):2215-2223. doi: 
            10.1534/g3.118.200144.
  PUBMED    29760202
REFERENCE   2  (bases 1 to 10240)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.118"; 
            journalName: "G3 (Bethesda)"; date: "2018-07-2- 2"; volume: "8"; 
            issue: "7"; pages: "2215-2223"
COMMENT     Sequence Label: DSCAM_pCSdest
FEATURES             Location/Qualifiers
     source          1..10240
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..978
                     /label=CMV IE94 promoter
                     /note="enhancer/promoter region of simian cytomegalovirus 
                     major immediate early transcription unit IE94"
     promoter        1013..1031
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     1013..1030
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     protein_bind    1085..1109
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(7202..7226)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    7270..7404
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7294..7313)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7348..7367
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(7517..7537)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(7517..7535)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7556..7572)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7556..7572)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7569..7591)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7580..7596
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7604..7610,7611..7628,7629..7634))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7649..7670
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7787..7804)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7958..8546)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8038..8057)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8717..9508,9509..9577))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9340..9359
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9578..9682)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(9708..10163)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9845..9866)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     10057..10076
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 gccaattcaa tatggcgtat atggactcat gccaattcaa tatggtggat ctggacctgt
       61 gccaattcaa tatggcgtat atggactcgt gccaattcaa tatggtggat ctggacccca
      121 gccaattcaa tatggcggac ttggcaccat gccaattcaa tatggcggac ctggcactgt
      181 gccaactggg gaggggtcta cttggcacgg tgccaagttt gaggaggggt cttggccctg
      241 tgccaagtcc gccatattga attggcatgg tgccaataat ggcggccata ttggctatat
      301 gccaggatca atatataggc aatatccaat atggccctat gccaatatgg ctattggcca
      361 ggttcaatac tatgtattgg ccctatgcca tatagtattc catatatggg ttttcctatt
      421 gacgtagata gcccctccca atgggcggtc ccatatacca tatatggggc ttcctaatac
      481 cgcccatagc cactccccca ttgacgtcaa tggtctctat atatggtctt tcctattgac
      541 gtcatatggg cggtcctatt gacgtatatg gcgcctcccc cattgacgtc aattacggta
      601 aatggcccgc ctggctcaat gcccattgac gtcaatagga ccacccacca ttgacgtcaa
      661 tgggatggct cattgcccat tcatatccgt tctcacgccc cctattgacg tcaatgacgg
      721 taaatggccc acttggcagt acatcaatat ctattaatag taacttggca agtacattac
      781 tattggaagt acgccagggt acattggcag tactcccatt gacgtcaatg gcggtaaatg
      841 gcccgcgatg gctgccaagt acatccccat tgacgtcaat ggggaggggc aatgacgcaa
      901 atgggcgttc cattgacgta aatgggcggt aggcgtgcct aatgggaggt ctatataagc
      961 aatgctcgtt tagggaaccg ccattctgcc tggggacgtc ggagcaagct tgatttaggt
     1021 gacactatag aatacaagct acttgttctt tttgcaggat cccatcgatt cgaattcaag
     1081 gatcacaagt ttgtacaaaa aagcaggctc cgcggccgcc cccttcacca tgtggatact
     1141 ggctctctcc ttgttccaga gcttcgcgaa tgttttcagt gaagacctac actccagcct
     1201 ctactttgtc aatgcatctc tgcaagaggt agtgtttgcc agcaccacgg ggactctggt
     1261 gccctgcccc gcagcaggca tccctcctgt gactctcaga tggtacctag ccacgggcga
     1321 ggagatctac gatgtccccg ggatccgcca cgtccacccc aacggcactc tccaaatttt
     1381 ccccttccct ccttcaagct tcagtacctt aatccatgat aatacttatt attgcacagc
     1441 tgaaaatcct tcagggaaaa ttagaagtca ggatgtccac atcaaggctg ttttacggga
     1501 gccctataca gtccgtgtgg aggaccagaa aaccatgaga ggcaatgttg cggtcttcaa
     1561 gtgcattatc ccctcctcgg tggaggcgta catcactgtc gtctcatggg agaaagacac
     1621 tgtttcactt gtctcaggat ctagatttct catcacatcc acgggagcct tgtatattaa
     1681 agatgtacag aatgaagatg gattgtataa ctaccgctgc atcacgcggc atcgatacac
     1741 cggagagacg aggcagagca acagcgccag actttttgta tcagacccag cgaactcagc
     1801 cccatccata ctggatgggt ttgagcatcg caaagccatg gctgggcagc gtgtggagct
     1861 gccttgcaaa gcgctcgggc accctgagcc agattaccgc tggctgaagg acaacatgcc
     1921 cctggaactt tcagggaggt tccagaagac cgtgacgggg ctgctcattg agaacattcg
     1981 cccctcggac tcaggcagct atgtttgtga agtgtccaac agatacggaa ctgctaaggt
     2041 gataggccgc ctgtacgtga aacagccact gaaagccacc atcagtccca ggaaggttaa
     2101 aagcagcgtg ggtagccaag tttccttgtc ctgcagcgtg acaggaactg aggaccagga
     2161 actctcctgg taccgcaatg gtgaaatcct caaccctgga aaaaatgtga ggatcacagg
     2221 gatcaaccac gaaaacctta taatggatca catggtcaaa agtgacgggg gcgcatacca
     2281 gtgctttgtg cgcaaggaca agctgtccgc tcaagactat gtgcaggtgg tccttgaaga
     2341 tggaactccc aaaattattt ctgcctttag tgaaaaggtg gtgagtccag cagagccggt
     2401 ttcccttatg tgcaacgtga agggaacacc tttgcccacg atcacgtgga ccctggacga
     2461 tgacccgatt ctcaagggtg gcagtcaccg catcagccag atgatcacgt cggaggggaa
     2521 cgtggtcagc tacctgaaca tctccagctc ccaggtccgg gacgggggag tctaccgctg
     2581 cactgccaac aactcggcgg gagtcgtcct gtaccaggct cgaataaacg taagagggcc
     2641 tgcaagcatt cgaccaatga aaaacatcac agcaatagca ggacgggaca catacattca
     2701 ctgtcgtgtg attggctatc cgtattactc cattaaatgg tacaagaact ctaacctgct
     2761 tcctttcaac caccgccaag tggcatttga gaacaatgga actcttaaac tttcagatgt
     2821 gcaaaaggaa gtggacgagg gggagtacac gtgcaacgtg ttggttcaac cacaactctc
     2881 caccagccag agcgtccacg tgaccgtgaa agttccgcct ttcatacaac cctttgagtt
     2941 tccaagattc tccattgggc agcgggtctt catcccctgt gttgtggtct caggggactt
     3001 acccatcacg atcacctggc agaaggatgg ccggccaatc cctgggagcc ttggggtgac
     3061 cattgacaat attgacttca cgagctcctt gaggatttcc aatctctcgc tcatgcacaa
     3121 tgggaattac acctgcatag cccggaatga ggccgccgct gtggagcacc aaagccagtt
     3181 gattgtcaga gttcctccca agtttgtggt tcagccacgg gaccaggacg ggatttatgg
     3241 caaagcagtc atcctcaatt gttctgctga gggttaccct gtacctacca tcgtgtggaa
     3301 attctctaaa ggtgctgggg ttccccagtt ccagccaatt gccctaaatg gccgaatcca
     3361 agttctcagc aatgggtcgt tgctgatcaa gcatgtcgtg gaggaagaca gtggctacta
     3421 cctctgcaag gtcagcaacg atgtgggcgc agacgtcagc aagtccatgt acctcacggt
     3481 taaaattcct gcgatgataa catcctatcc aaatactacc ctggccacgc aggggcagaa
     3541 aaaggagatg agctgcacgg cgcatggtga gaagcccatt atagtccgct gggagaagga
     3601 ggaccgaatc attaaccctg agatggcccg ttatcttgtg tccaccaagg aggtgggaga
     3661 agaggtgatt tctactctgc agattttgcc aactgtgaga gaagattctg gtttcttttc
     3721 ctgccatgct attaattctt atggggagga ccgtggaata attcagctca cagtgcaaga
     3781 gcccccagac cctcccgaaa ttgagatcaa agatgtcaaa gcacgcacaa ttacgctcag
     3841 gtggaccatg gggtttgatg gaaacagtcc catcacaggc tacgatattg aatgcaaaaa
     3901 taaatcagac tcctgggatt ctgctcagag aaccaaagat gtttcccctc agctgaactc
     3961 ggccaccatc attgatatcc acccttcctc cacctacagc atccgcatgt acgccaagaa
     4021 ccggattggc aagagcgagc ccagcaacga gctcaccatc acggcggacg aggcagctcc
     4081 tgatggtcca cctcaggaag ttcacctgga gcctatatca tctcagagca tcagggtcac
     4141 atggaaggct cccaagaaac atttgcaaaa tgggattatc cgtggctacc aaataggtta
     4201 ccgagagtac agcactgggg gtaacttcca attcaacatt atcagtgtcg acaccagcgg
     4261 ggacagtgag gtttacaccc tggacaacct gaataagttc actcagtacg gcctggtggt
     4321 gcaggcctgt aaccgggccg gcacggggcc ttcttctcag gaaatcatca ccaccactct
     4381 cgaggatgtg cccagttacc cccccgaaaa tgtccaagcc atagcaacat caccagaaag
     4441 catatcaata tcctggtcca cactttccaa ggaagccttg aatggaattc tccaggggtt
     4501 cagagtcatt tactgggcca acctcatgga cggagagctg ggtgagatta aaaacatcac
     4561 caccacacag ccttcactgg agctggacgg gctggaaaag tacaccaact acagcatcca
     4621 ggtgctggcc ttcacccgcg caggagacgg ggtcaggagt gagcagatct tcacccggac
     4681 caaagaggat gttccaggtc ctcccgcggg tgtgaaggca gcggcggcct cagcctccat
     4741 ggtctttgtg tcctggcttc cccctctcaa gctgaacggc atcatccgaa agtacactgt
     4801 attctgctcc cacccctatc ccacagtgat cagcgagttt gaggcctctc ccgactcgtt
     4861 ttcctacaga attcccaacc tgagtaggaa tcgtcagtac agcgtctggg tggtggctgt
     4921 tacttcagcc ggaagaggca acagcagtga aatcatcaca gttgagccac tagcaaaagc
     4981 tcctgcacga atcctgacct tcagtgggac agtgactact ccatggatga aagacattgt
     5041 cttgccttgt aaggctgttg gggacccttc tcctgcagtc aaatggatga aagacagtaa
     5101 cgggacaccc agtctagtaa cgattgatgg gcggaggagc atctttagca acggaagctt
     5161 cattattcgc acggtgaaag cagaagactc cggctattac agctgcattg ccaataacaa
     5221 ctggggatct gatgaaatta ttttaaactt acaagtacaa gttccaccag atcagcctcg
     5281 gcttacagtc tccaagacca cgtcttcctc catcaccctt tcttggctcc ctggagacaa
     5341 cgggggcagc tctatcagag gatacatact gcagtactcc gaggacaata gtgagcagtg
     5401 ggggagtttt ccaatcagcc ccagcgaacg ttcctatcgc ttggaaaatc tcaaatgtgg
     5461 gacttggtat aagttcacac tgacagccca aaatggagtg ggcccagggc gcataagtga
     5521 aatcatagaa gcaaagacct taggaaaaga gccccagttc tcaaaggagc aggagctgtt
     5581 tgccagcatc aacaccacac gcgtgaggct gaacctcatt ggctggaatg atggcggctg
     5641 ccccatcacc tccttcacac tagagtacag gccctttggg accacagttt ggaccacagc
     5701 tcagaggacc tctctctcca agtcctacat cctgtatgac ctgcaggaag ccacctggta
     5761 tgagctgcag atgcgggtgt gcaacagtgc gggctgcgcg gagaagcagg ccaacttcgc
     5821 tacgctgaac tacgatggca gtacaattcc tccactcatt aagtcagttg tccaaaacga
     5881 agaagggctg acgaccaacg aggggctcaa gatgctggtg accatctcct gtatcctggt
     5941 gggggtcttg ctgctgtttg tgctcctgct ggttgtgcgg aggaggcggc gggagcagag
     6001 gctaaagagg ctgcgagatg caaagagttt agctgaaatg ctcatgagta agaatacccg
     6061 gacttcagat acgttaagca agcaacagca gaccctgcga atgcacatcg acatacccag
     6121 ggctcagctt ttgattgaag agagagacac gatggagacc attgatgatc gctccacggt
     6181 tctgttgacg gatgctgact ttggagaggc agctaagcag aagtccctga cggtcactca
     6241 cacggtccat taccaatcgg tgtctcaggc cactgggccc ttagtggatg tttcagacgc
     6301 tcggccggga acgaatccca ccaccaggag gaatgccaag gctgggccca cagcgagaaa
     6361 ccgctatgcc agccagtgga ccctcaaccg accccacccc accatctcag cacacaccct
     6421 caccacagac tggaggctgc caacacccag ggctgcagga tcagtagaca aagagagcga
     6481 cagttacagc gtcagcccct cgcaagacac agatcgagca agaagcagca tggtctccac
     6541 agaaagtgcc tcctccactt acgaagaact ggccagggcc tacgaacacg ccaagatgga
     6601 agagcaactg aggcacgcca agttcaccat cacggagtgc ttcatatcag acacgtcatc
     6661 ggagcagttg acggcaggga caaatgagta cacggacagt ctgacctcca gcaccccttc
     6721 cgaatcggga atctgcaggt tcactgcatc tccccccaaa cctcaggatg gaggaagagt
     6781 aatgaatatg gcagttccaa aggcacatcg gccaggtgac ctcatacatt tgcctccata
     6841 ccttagaatg gactttttgt taaaccgagg tggtccaggc accagcaggg acctgagctt
     6901 aggacaagca tgcttggaac ctcagaaaag ccggaccctg aagcgcccca cggtcctgga
     6961 gcccatcccg atggaagccg cctcctccgc ctcctccacg agagaaggac agtcgtggca
     7021 gccgggggcc gtggccacat tacctcagcg ggagggagca gagctgggac aggcagctaa
     7081 aatgagcagc tcccaagaat cactgctcga ctcccggggc catttgaaag gaaacaatcc
     7141 ttacgcaaaa tcttacaccc tggtagttta aacgaattcg agctcaaggg tgggcgcgcc
     7201 gacccagctt tcttgtacaa agtggtgatc ctctcgagcc tctagaacta tagtgagtcg
     7261 tattacgtag atccagacat gataagatac attgatgagt ttggacaaac cacaactaga
     7321 atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc
     7381 attataagct gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt
     7441 cagggggagg tgtgggaggt tttttaattc gcggccgcgg cgccaatgca ttgggcccgg
     7501 tacccagctt ttgttccctt tagtgagggt taattgcgcg cttggcgtaa tcatggtcat
     7561 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggga
     7621 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
     7681 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
     7741 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
     7801 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     7861 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     7921 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     7981 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     8041 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     8101 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     8161 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     8221 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     8281 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     8341 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
     8401 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     8461 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     8521 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     8581 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     8641 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     8701 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     8761 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     8821 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     8881 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     8941 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     9001 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     9061 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     9121 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     9181 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     9241 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     9301 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
     9361 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     9421 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     9481 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     9541 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     9601 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     9661 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctaaa ttgtaagcgt
     9721 taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata
     9781 ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt
     9841 tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg
     9901 aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt
     9961 ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc
    10021 ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg
    10081 cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct
    10141 taatgcgccg ctacagggcg cgtcccattc gccattcagg ctgcgcaact gttgggaagg
    10201 gcgatcggtg cgggcctctt cgctattacg ccagtcgacc
//
