LOCUS       sequence-313227-       10108 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Doxycycline-dependently expresses human BCL2 and murine miR9/9*-124.
            Ubiquitously expresses iRFP713, Puromycin resistance gene, and 
            rtTAM2..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10108)
  AUTHORS   Nemoto A, Kobayashi R, Yoshimatsu S, Sato Y, Kondo T, Yoo AS, 
            Shiozawa S, Okano H
  TITLE     Direct Neuronal Reprogramming of Common Marmoset Fibroblasts by 
            ASCL1, microRNA-9/9*, and microRNA-124 Overexpression.
  JOURNAL   Cells. 2020 Dec 22;10(1). pii: cells10010006. doi: 
            10.3390/cells10010006.
  PUBMED    33375083
REFERENCE   2  (bases 1 to 10108)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cells."; 
            date: "2020-12-22"; volume: "10(1). pii: cells10010006. doi"; pages:
            " 10.3390/cells10010006"
COMMENT     Sequence Label: pPB-tetpA(hBCL2-miR9/9*-124)-iRPT
FEATURES             Location/Qualifiers
     source          1..10108
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..17
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     24..43
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        24..42
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   61..95
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     polyA_signal    370..577
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    588..608
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    1264..1288
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2027..2051)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(2061..2375)
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     primer_bind     complement(2064..2088)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    2135..2153
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2171..2189
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2206..2224
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2242..2260
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2278..2296
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2313..2331
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2349..2367
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        2412..3574
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2643..3565
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3522..3542
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      3587..3596
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4541..4594
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             4601..5197
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4601..4620)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5097..5117
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             5198..5254
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             join(5255..5872,5873..5878,5879..5914,5915..5917,5918..5953,
                     5954..5956,5957..5992,5993..6001)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    6051..6639
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6104..6124)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6522..6533)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    6925..7046
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6962..6981)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7016..7035
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   7235..7297
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        complement(7321..7339)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7360..7376)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7360..7376)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7373..7395)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7384..7400
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7408..7414,7415..7432,7433..7438))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7453..7474
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7591..7608)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7762..8350)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7842..7861)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8521..9312,9313..9381))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9144..9163
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9382..9486)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(9512..9967)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9649..9670)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9861..9880
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     join(10094..10108,1..8)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     join(10108,1..17)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
ORIGIN
        1 gtaaaacgac ggccagtgaa ttgtaatacg actcactata gggcgaattg ggtaccttaa
       61 ccctagaaag atagtctgcg taaaattgac gcatgcattc ttgaaatatt gctctctctt
      121 tctaaatagc gcgaatccgt cgctgtgcat ttaggacatc tcagtcgccg cttggagctc
      181 ccgtgaggcg tgcttgtcaa tgcggtaagt gtcactgatt ttgaactata acgaccgcgt
      241 gagtcaaaat gacgcatgat tatcttttac gtgactttta agatttaact catacgataa
      301 ttatattgtt atttcatgtt ctacttacgt gataacttat tatatatata ttttcttgtt
      361 atagatatct ccccagcatg cctgctattg tcttcccaat cctccccctt gctgtcctgc
      421 cccaccccac cccccagaat agaatgacac ctactcagac aatgcgatgc aatttcctca
      481 ttttattagg aaaggacagt gggagtggca ccttccaggg tcaaggaagg cacgggggag
      541 gggcaaacaa cagatggctg gcaactagaa ggcacagatc aagctatcaa ctttgtatag
      601 aaaagttggt gtcgcacagg atgggttcat tctagaattg tttctcccca cccttcctaa
      661 cttctctcgg atgtccttca agtgcagctg taggctccgc tcttggcatt caccgcgtgc
      721 cttaattgta tgacattaaa tcaaggtccg ctgtgaacac ggagagcaga gtctctgatc
      781 ttgatctaga agcaagtgac tcattgcaac tattactata attttctccc tgcccttcac
      841 cctctcttgg agctctaggc ttggagtccc tactcctaga gttgtagctc tgagcagtac
      901 tcccacctac tagaaggaga gccgaggtcg gatacctggt cccggcgtgg ccgctccgtc
      961 gagctggaat ccctgggtgg cgtgggcttc tctgggctcc tctggctcct gtggccaggg
     1021 cggccggccg ggccatcgca cgcagaagtt gtgagagtca tttctacttt cggttatcta
     1081 gctttatgac ggctctgtgg cactcataca gctagataac caaagagaga aacgggcctc
     1141 ccattcgccg gcaccgctgc cgcgctgtgg gccccacgtg ccgccgccga cccggcgcgg
     1201 gggcctgagc ggactccgct cccgcagccc cgggcgcacg cggagagcca gaggggaccg
     1261 acagccactt tgtacaagaa agctgggtct tacttgtggc ccagataggc acccagggtg
     1321 atgcaagctc ccaccagggc caaactgagc agagtcttca gagacagcca ggagaaatca
     1381 aacagaggcc gcatgctggg gccgtacagt tccacaaagg catcccagcc tccgttatcc
     1441 tggatccagg tgtgcaggtg ccggttcagg tactcagtca tccacagggc gatgttgtcc
     1501 accaggggcg acatctcccg gttgacgctc tccacacaca tgaccccacc gaactcaaag
     1561 aaggccacaa tcctccccca gttcaccccg tccctgaaga gctcctccac caccgtggca
     1621 aagcgtcccc gcgcggtgaa gggcgtcagg tgcagctggc tggacatctc ggcgaagtcg
     1681 cggcggtagc ggcgggagaa gtcgtcgccg gcctggcgga gggtcaggtg gaccacaggt
     1741 ggcaccgggc tgagcgcagg ccccgcggcg gcgccggggg cagccggggt ctgcagcggc
     1801 gaggtcctgg cgaccgggtc ccgggatgcg gctggatggg gcgtgtgccc gggctgggag
     1861 gagaagatgc ccggtgcggg ggcggccccc gggggcgcgg cgcccacatc tcccgcatcc
     1921 cactcgtagc ccctctgcga cagcttataa tggatgtact tcatcactat ctcccggtta
     1981 tcgtaccccg ttctcccagc gtgcgccatg gttctatctc cttcgaagcc tgcttttttg
     2041 tacaaacttg tgatgctagc ggcgatctga cggttcacta aacgagctct gcttatatag
     2101 gcctcccacc gtacacgcct acctcgacat acgttctcta tcactgatag ggagtaaact
     2161 cgacatacgt tctctatcac tgatagggat aaactcgaca tacgttctct atcactgata
     2221 gggagtaaac tcgacatacg ttctctatca ctgataggga gtaaactcga catacgttct
     2281 ctatcactga tagggagtaa actcgacatc gttctctatc actgataggg agtaaactcg
     2341 acatacgttc tctatcactg atagggagta aactcgattg aagacgaaag ggccgaattc
     2401 aagcttcgtg aggctccggt gcccgtcagt gggcagagcg cacatcgccc acagtccccg
     2461 agaagttggg gggaggggtc ggcaattgaa ccggtgccta gagaaggtgg cgcggggtaa
     2521 actgggaaag tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt
     2581 atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac
     2641 aggtaagtgc cgtgtgtggt tcccgcgggc ctggcctctt tacgggttat ggcccttgcg
     2701 tgccttgaat tacttccacc tggctccagt acgtgattct tgatcccgag ctggagccag
     2761 gggcgggcct tgcgctttag gagccccttc gcctcgtgct tgagttgagg cctggcctgg
     2821 gcgctggggc cgccgcgtgc gaatctggtg gcaccttcgc gcctgtctcg ctgctttcga
     2881 taagtctcta gccatttaaa atttttgatg acctgctgcg acgctttttt tctggcaaga
     2941 tagtcttgta aatgcgggcc aggatctgca cactggtatt tcggtttttg gggccgcggg
     3001 cggcgacggg gcccgtgcgt cccagcgcac atgttcggcg aggcggggcc tgcgagcgcg
     3061 gccaccgaga atcggacggg ggtagtctca agctggccgg cctgctctgg tgcctggcct
     3121 cgcgccgccg tgtatcgccc cgccctgggc ggcaaggctg gcccggtcgg caccagttgc
     3181 gtgagcggaa agatggccgc ttcccggccc tgctccaggg ggctcaaaat ggaggacgcg
     3241 gcgctcggga gagcgggcgg gtgagtcacc cacacaaagg aaaggggcct ttccgtcctc
     3301 agccgtcgct tcatgtgact ccacggagta ccgggcgccg tccaggcacc tcgattagtt
     3361 ctggagcttt tggagtacgt cgtctttagg ttggggggag gggttttatg cgatggagtt
     3421 tccccacact gagtgggtgg agactgaagt taggccagct tggcacttga tgtaattctc
     3481 cttggaattt gccctttttg agtttggatc ttggttcatt ctcaagcctc agacagtggt
     3541 tcaaagtttt tttcttccat ttcaggtgtc gtgaacacgc taccgggcca ccatggccga
     3601 agggagcgtg gcaagacagc ccgatctgct gacatgcgac gacgaaccaa tccacattcc
     3661 aggagctatc cagcctcatg gactgctgct ggctctggcc gctgacatga ctatcgtggc
     3721 aggctctgat aacctgccag agctgaccgg gctggctatc ggagcactga ttggccggtc
     3781 cgcagccgac gtgttcgatt ctgagactca caatagactg accatcgccc tggctgaacc
     3841 tggggctgca gtgggagccc caattaccgt cggcttcaca atgcgaaagg acgctggctt
     3901 tatcgggtcc tggcaccgcc atgatcagct gatttttctg gagctggaac cacctcagag
     3961 agacgtggca gagccacagg ctttctttcg gagaacaaac agcgccatca ggcgcctgca
     4021 ggccgctgag actctggaat ccgcttgcgc agccgctgca caggaagtga ggaagatcac
     4081 cgggttcgat cgcgtcatga tctacagatt cgcctcagac tttagcggag aagtgatcgc
     4141 agaagatcgc tgtgccgagg tcgaaagtaa actgggcctg cattacccag cctcaaccgt
     4201 gcctgcacag gcacgacggc tgtatacaat caaccccgtg cggatcattc ccgacattaa
     4261 ttacagacca gtgcccgtca cacctgacct gaacccagtg actgggaggc ccatcgatct
     4321 gagcttcgcc attctgcgca gcgtgtcccc tgtccacctg gagtttatga ggaatatcgg
     4381 aatgcatggc accatgtcta tcagtattct gagaggcgaa aggctgtggg ggctgatcgt
     4441 gtgccaccat cggacacctt actatgtgga cctggacggc aggcaggctt gtgaactggt
     4501 ggctcaggtg ctggcttggc agattggcgt gatggaagaa gagggcagag gaagtcttct
     4561 aacatgcggt gacgtggagg agaatcccgg ccctatgtct atgaccgagt acaagcccac
     4621 ggtgcgcctc gccacccgcg acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt
     4681 cgccgactac cccgccacgc gccacaccgt cgatccggac cgccacatcg agcgggtcac
     4741 cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc
     4801 ggacgacggc gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt
     4861 gttcgccgag atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca
     4921 acagatggaa ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac
     4981 cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg
     5041 agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa
     5101 cctccccttc tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg
     5161 accgcgcacc tggtgcatga cccgcaagcc cggtgccgcc acgaacttct ctctgttaaa
     5221 gcaagcagga gacgtggaag aaaaccccgg tcctatgtct agactggaca agagcaaagt
     5281 cataaacggc gctctggaat tactcaatgg agtcggtatc gaaggcctga cgacaaggaa
     5341 actcgctcaa aagctgggag ttgagcagcc taccctgtac tggcacgtga agaacaagcg
     5401 ggccctgctc gatgccctgc caatcgagat gctggacagg catcataccc acttctgccc
     5461 cctggaaggc gagtcatggc aagactttct gcggaacaac gccaagtcat tccgctgtgc
     5521 tctcctctca catcgcgacg gggctaaagt gcatctcggc acccgcccaa cagagaaaca
     5581 gtacgaaacc ctggaaaatc agctcgcgtt cctgtgtcag caaggcttct ccctggagaa
     5641 cgcactgtac gctctgtccg ccgtgggcca ctttacactg ggctgcgtat tggaggaaca
     5701 ggagcatcaa gtagcaaaag aggaaagaga gacacctacc accgattcta tgcccccact
     5761 tctgagacaa gcaattgagc tgttcgaccg gcagggagcc gaacctgcct tccttttcgg
     5821 cctggaacta atcatatgtg gcctggagaa acagctaaag tgcgaaagcg gcgggccggc
     5881 cgacgccctt gacgattttg acttagacat gctcccagcc gatgcccttg acgactttga
     5941 ccttgatatg ctgcctgctg acgctcttga cgattttgac cttgacatgc tccccgggta
     6001 actaagtaag gatcctctag agttaacatc gagggatcaa gcttatcgat aatcaacctc
     6061 tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc
     6121 tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca
     6181 ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg
     6241 tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca
     6301 ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg
     6361 cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg
     6421 acaattccgt ggtgttgtcg gggaagctga cgtcctttcc atggctgctc gcctgtgttg
     6481 ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg
     6541 accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc
     6601 ctcagacgag tcggatctcc ctttgggccg cctccccgca tcgataccgt cgagacctag
     6661 aaaaacatgg agcaatcaca agtagcaata cagcagctac caatgctgat tgtgcctggc
     6721 tagaagcaca agaggaggag gaggtgggtt ttccagtcac acctcaggta cctttaagac
     6781 caatgactta caaggcagct gtagatctct agataactga tcataatcag ccataccaca
     6841 tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat
     6901 aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa
     6961 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     7021 ttgtccaaac tcatcaatgt atcttaacgc tcgagtgcat ggatcctttg ttactttata
     7081 gaagaaattt tgagtttttg ttttttttta ataaataaat aaacataaat aaattgtttg
     7141 ttgaatttat tattagtatg taagtgtaaa tataataaaa cttaatatct attcaaatta
     7201 ataaataaac ctcgatatac agaccgataa aacacatgcg tcaattttac gcatgattat
     7261 ctttaacgta cgtcacaata tgattatctt tctagggtta aagagctcca gcttttgttc
     7321 cctttagtga gggttaattt cgagcttggc gtaatcatgg tcatagctgt ttcctgtgtg
     7381 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
     7441 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
     7501 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
     7561 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     7621 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     7681 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     7741 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     7801 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     7861 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     7921 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     7981 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     8041 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     8101 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     8161 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
     8221 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     8281 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     8341 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     8401 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     8461 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     8521 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     8581 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     8641 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     8701 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     8761 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     8821 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     8881 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     8941 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     9001 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     9061 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     9121 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
     9181 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     9241 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     9301 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     9361 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
     9421 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     9481 tccgcgcaca tttccccgaa aagtgccacc taaattgtaa gcgttaatat tttgttaaaa
     9541 ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa
     9601 atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac
     9661 aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag
     9721 ggcgatggcc cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt
     9781 aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg
     9841 gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca
     9901 agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag
     9961 ggcgcgtccc attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc
    10021 tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta
    10081 acgccagggt tttcccagtc acgacgtt
//
