LOCUS       sequence-313231-       10371 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Ubiquitously expresses iRFP713, Puromycin resistance gene, and 
            rtTAM2..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10371)
  AUTHORS   Nemoto A, Kobayashi R, Yoshimatsu S, Sato Y, Kondo T, Yoo AS, 
            Shiozawa S, Okano H
  TITLE     Direct Neuronal Reprogramming of Common Marmoset Fibroblasts by 
            ASCL1, microRNA-9/9*, and microRNA-124 Overexpression.
  JOURNAL   Cells. 2020 Dec 22;10(1). pii: cells10010006. doi: 
            10.3390/cells10010006.
  PUBMED    33375083
REFERENCE   2  (bases 1 to 10371)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cells."; 
            date: "2020-12-22"; volume: "10(1). pii: cells10010006. doi"; pages:
            " 10.3390/cells10010006"
COMMENT     Sequence Label: pPB-tetpA(R1R4)-iRPT
FEATURES             Location/Qualifiers
     source          1..10371
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(28..456)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(138..159)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     350..369
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     583..605
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     597..614
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     598..614
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     621..640
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        621..639
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   658..692
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     polyA_signal    967..1174
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    1185..1308
                     /gene="mutant version of attR"
                     /label=attR4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(1372..1677)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(1474..1493)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     CDS             complement(2019..2678)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2593..2612
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(join(2732..2738,2739..2756,2757..2762))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(2787..2911)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(2921..3235)
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     primer_bind     complement(2924..2948)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    2995..3013
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3031..3049
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3066..3084
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3102..3120
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3138..3156
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3173..3191
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3209..3227
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        3272..4434
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3503..4425
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4382..4402
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      4447..4456
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5401..5454
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             5461..6057
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5461..5480)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5957..5977
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             6058..6114
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             join(6115..6732,6733..6738,6739..6774,6775..6777,6778..6813,
                     6814..6816,6817..6852,6853..6861)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    6911..7499
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6964..6984)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7382..7393)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    7785..7906
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7822..7841)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7876..7895
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   8095..8157
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        complement(8181..8199)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(8220..8236)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8220..8236)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8233..8255)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8244..8260
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8268..8274,8275..8292,8293..8298))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8313..8334
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8451..8468)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8622..9210)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8702..8721)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9381..10172,10173..10241))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10004..10023
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10242..10346)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat
       61 tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga
      121 tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca
      181 acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct
      241 aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc
      301 cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag
      361 cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca
      421 cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc aggctgcgca
      481 actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg
      541 gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta
      601 aaacgacggc cagtgaattg taatacgact cactataggg cgaattgggt accttaaccc
      661 tagaaagata gtctgcgtaa aattgacgca tgcattcttg aaatattgct ctctctttct
      721 aaatagcgcg aatccgtcgc tgtgcattta ggacatctca gtcgccgctt ggagctcccg
      781 tgaggcgtgc ttgtcaatgc ggtaagtgtc actgattttg aactataacg accgcgtgag
      841 tcaaaatgac gcatgattat cttttacgtg acttttaaga tttaactcat acgataatta
      901 tattgttatt tcatgttcta cttacgtgat aacttattat atatatattt tcttgttata
      961 gatatctccc cagcatgcct gctattgtct tcccaatcct cccccttgct gtcctgcccc
     1021 accccacccc ccagaataga atgacaccta ctcagacaat gcgatgcaat ttcctcattt
     1081 tattaggaaa ggacagtggg agtggcacct tccagggtca aggaaggcac gggggagggg
     1141 caaacaacag atggctggca actagaaggc acagatcaag ctatcaactt tgtatagaaa
     1201 agttgaacga gaaacgtaaa atgatataaa tatcaatata ttaaattaga ttttgcataa
     1261 aaaacagact acataatact gtaaaacaca acatatccag tcactatggt cgacctgcag
     1321 actggctgtg tgtcgacctg cagactggct gtgtataagg gagcctgaca tttatattcc
     1381 ccagaacatc aggttaatgg cgtttttgat gtcattttcg cggtggctga gatcagccac
     1441 ttcttccccg ataacggaga ccggcacact ggccatatcg gtggtcatca tgcgccagct
     1501 ttcatccccg atatgcacca ccgggtaaag ttcacgggag actttatctg acagcagacg
     1561 tgcactggcc agggggatca ccatccgtcg cccgggcgtg tcaataatat cactctgtac
     1621 atccacaaac agacgataac ggctctctct tttataggtg taaaccttaa actgcatttc
     1681 accagcccct gttctcgtca gcaaaagagc cgttcatttc aataaaccgg gcgacctcag
     1741 ccatcccttc ctgattttcc gctttccagc gttcggcacg cagacgacgg gcttcattct
     1801 gcatggttgt gcttaccaga ccggagatat tgacatcata tatgccttga gcaactgata
     1861 gctgtcgctg tcaactgtca ctgtaatacg ctgcttcata gcatacctct ttttgacata
     1921 cttcgggtat acatatcagt atatattctt ataccgcaaa aatcagcgcg caaatacgca
     1981 tactgttatc tggcttttag taagccggat ccacgcgttt acgccccgcc ctgccactca
     2041 tcgcagtact gttgtaattc attaagcatt ctgccgacat ggaagccatc acaaacggca
     2101 tgatgaacct gaatcgccag cggcatcagc accttgtcgc cttgcgtata atatttgccc
     2161 atggtgaaaa cgggggcgaa gaagttgtcc atattggcca cgtttaaatc aaaactggtg
     2221 aaactcaccc agggattggc tgagacgaaa aacatattct caataaaccc tttagggaaa
     2281 taggccaggt tttcaccgta acacgccaca tcttgcgaat atatgtgtag aaactgccgg
     2341 aaatcgtcgt ggtattcact ccagagcgat gaaaacgttt cagtttgctc atggaaaacg
     2401 gtgtaacaag ggtgaacact atcccatatc accagctcac cgtctttcat tgccatacgg
     2461 aattccggat gagcattcat caggcgggca agaatgtgaa taaaggccgg ataaaacttg
     2521 tgcttatttt tctttacggt ctttaaaaag gccgtaatat ccagctgaac ggtctggtta
     2581 taggtacatt gagcaactga ctgaaatgcc tcaaaatgtt ctttacgatg ccattgggat
     2641 atatcaacgg tggtatatcc agtgattttt ttctccattt tagcttcctt agctcctgaa
     2701 aatctcgacg gatcctaact caaaatccac acattatacg agccggaagc ataaagtgta
     2761 aagcctgggg tgcctaatgc ggccgccata gtgactggat atgttgtgtt ttacagtatt
     2821 atgtagtctg ttttttatgc aaaatctaat ttaatatatt gatatttata tcattttacg
     2881 tttctcgttc agcttttttg tacaaacttg tgatgctagc ggcgatctga cggttcacta
     2941 aacgagctct gcttatatag gcctcccacc gtacacgcct acctcgacat acgttctcta
     3001 tcactgatag ggagtaaact cgacatacgt tctctatcac tgatagggat aaactcgaca
     3061 tacgttctct atcactgata gggagtaaac tcgacatacg ttctctatca ctgataggga
     3121 gtaaactcga catacgttct ctatcactga tagggagtaa actcgacatc gttctctatc
     3181 actgataggg agtaaactcg acatacgttc tctatcactg atagggagta aactcgattg
     3241 aagacgaaag ggccgaattc aagcttcgtg aggctccggt gcccgtcagt gggcagagcg
     3301 cacatcgccc acagtccccg agaagttggg gggaggggtc ggcaattgaa ccggtgccta
     3361 gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg tactggctcc gcctttttcc
     3421 cgagggtggg ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa
     3481 cgggtttgcc gccagaacac aggtaagtgc cgtgtgtggt tcccgcgggc ctggcctctt
     3541 tacgggttat ggcccttgcg tgccttgaat tacttccacc tggctccagt acgtgattct
     3601 tgatcccgag ctggagccag gggcgggcct tgcgctttag gagccccttc gcctcgtgct
     3661 tgagttgagg cctggcctgg gcgctggggc cgccgcgtgc gaatctggtg gcaccttcgc
     3721 gcctgtctcg ctgctttcga taagtctcta gccatttaaa atttttgatg acctgctgcg
     3781 acgctttttt tctggcaaga tagtcttgta aatgcgggcc aggatctgca cactggtatt
     3841 tcggtttttg gggccgcggg cggcgacggg gcccgtgcgt cccagcgcac atgttcggcg
     3901 aggcggggcc tgcgagcgcg gccaccgaga atcggacggg ggtagtctca agctggccgg
     3961 cctgctctgg tgcctggcct cgcgccgccg tgtatcgccc cgccctgggc ggcaaggctg
     4021 gcccggtcgg caccagttgc gtgagcggaa agatggccgc ttcccggccc tgctccaggg
     4081 ggctcaaaat ggaggacgcg gcgctcggga gagcgggcgg gtgagtcacc cacacaaagg
     4141 aaaggggcct ttccgtcctc agccgtcgct tcatgtgact ccacggagta ccgggcgccg
     4201 tccaggcacc tcgattagtt ctggagcttt tggagtacgt cgtctttagg ttggggggag
     4261 gggttttatg cgatggagtt tccccacact gagtgggtgg agactgaagt taggccagct
     4321 tggcacttga tgtaattctc cttggaattt gccctttttg agtttggatc ttggttcatt
     4381 ctcaagcctc agacagtggt tcaaagtttt tttcttccat ttcaggtgtc gtgaacacgc
     4441 taccgggcca ccatggccga agggagcgtg gcaagacagc ccgatctgct gacatgcgac
     4501 gacgaaccaa tccacattcc aggagctatc cagcctcatg gactgctgct ggctctggcc
     4561 gctgacatga ctatcgtggc aggctctgat aacctgccag agctgaccgg gctggctatc
     4621 ggagcactga ttggccggtc cgcagccgac gtgttcgatt ctgagactca caatagactg
     4681 accatcgccc tggctgaacc tggggctgca gtgggagccc caattaccgt cggcttcaca
     4741 atgcgaaagg acgctggctt tatcgggtcc tggcaccgcc atgatcagct gatttttctg
     4801 gagctggaac cacctcagag agacgtggca gagccacagg ctttctttcg gagaacaaac
     4861 agcgccatca ggcgcctgca ggccgctgag actctggaat ccgcttgcgc agccgctgca
     4921 caggaagtga ggaagatcac cgggttcgat cgcgtcatga tctacagatt cgcctcagac
     4981 tttagcggag aagtgatcgc agaagatcgc tgtgccgagg tcgaaagtaa actgggcctg
     5041 cattacccag cctcaaccgt gcctgcacag gcacgacggc tgtatacaat caaccccgtg
     5101 cggatcattc ccgacattaa ttacagacca gtgcccgtca cacctgacct gaacccagtg
     5161 actgggaggc ccatcgatct gagcttcgcc attctgcgca gcgtgtcccc tgtccacctg
     5221 gagtttatga ggaatatcgg aatgcatggc accatgtcta tcagtattct gagaggcgaa
     5281 aggctgtggg ggctgatcgt gtgccaccat cggacacctt actatgtgga cctggacggc
     5341 aggcaggctt gtgaactggt ggctcaggtg ctggcttggc agattggcgt gatggaagaa
     5401 gagggcagag gaagtcttct aacatgcggt gacgtggagg agaatcccgg ccctatgtct
     5461 atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc cagggccgta
     5521 cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgatccggac
     5581 cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac
     5641 atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag
     5701 agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt
     5761 tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag
     5821 cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc
     5881 agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg
     5941 gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc
     6001 gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgccgcc
     6061 acgaacttct ctctgttaaa gcaagcagga gacgtggaag aaaaccccgg tcctatgtct
     6121 agactggaca agagcaaagt cataaacggc gctctggaat tactcaatgg agtcggtatc
     6181 gaaggcctga cgacaaggaa actcgctcaa aagctgggag ttgagcagcc taccctgtac
     6241 tggcacgtga agaacaagcg ggccctgctc gatgccctgc caatcgagat gctggacagg
     6301 catcataccc acttctgccc cctggaaggc gagtcatggc aagactttct gcggaacaac
     6361 gccaagtcat tccgctgtgc tctcctctca catcgcgacg gggctaaagt gcatctcggc
     6421 acccgcccaa cagagaaaca gtacgaaacc ctggaaaatc agctcgcgtt cctgtgtcag
     6481 caaggcttct ccctggagaa cgcactgtac gctctgtccg ccgtgggcca ctttacactg
     6541 ggctgcgtat tggaggaaca ggagcatcaa gtagcaaaag aggaaagaga gacacctacc
     6601 accgattcta tgcccccact tctgagacaa gcaattgagc tgttcgaccg gcagggagcc
     6661 gaacctgcct tccttttcgg cctggaacta atcatatgtg gcctggagaa acagctaaag
     6721 tgcgaaagcg gcgggccggc cgacgccctt gacgattttg acttagacat gctcccagcc
     6781 gatgcccttg acgactttga ccttgatatg ctgcctgctg acgctcttga cgattttgac
     6841 cttgacatgc tccccgggta actaagtaag gatcctctag agttaacatc gagggatcaa
     6901 gcttatcgat aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa
     6961 ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat
     7021 tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta
     7081 tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc
     7141 aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt
     7201 ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg
     7261 ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaagctga cgtcctttcc
     7321 atggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc
     7381 ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct
     7441 tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgca
     7501 tcgataccgt cgagacctag aaaaacatgg agcaatcaca agtagcaata cagcagctac
     7561 caatgctgat tgtgcctggc tagaagcaca agaggaggag gaggtgggtt ttccagtcac
     7621 acctcaggta cctttaagac caatgactta caaggcagct gtagatctct agataactga
     7681 tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc
     7741 tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag
     7801 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     7861 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttaacgc tcgagtgcat
     7921 ggatcctttg ttactttata gaagaaattt tgagtttttg ttttttttta ataaataaat
     7981 aaacataaat aaattgtttg ttgaatttat tattagtatg taagtgtaaa tataataaaa
     8041 cttaatatct attcaaatta ataaataaac ctcgatatac agaccgataa aacacatgcg
     8101 tcaattttac gcatgattat ctttaacgta cgtcacaata tgattatctt tctagggtta
     8161 aagagctcca gcttttgttc cctttagtga gggttaattt cgagcttggc gtaatcatgg
     8221 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
     8281 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
     8341 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
     8401 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
     8461 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     8521 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     8581 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     8641 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     8701 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     8761 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     8821 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     8881 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     8941 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     9001 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     9061 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     9121 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     9181 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     9241 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     9301 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     9361 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     9421 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     9481 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     9541 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     9601 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     9661 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     9721 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     9781 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     9841 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     9901 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     9961 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
    10021 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
    10081 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
    10141 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
    10201 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
    10261 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
    10321 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc t
//
