LOCUS       sequence-313240-       12790 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Episomally expresses KDM4D and GLIS1 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12790)
  AUTHORS   Yoshimatsu S, Nakajima M, Iguchi A, Sanosaka T, Sato T, Nakamura M, 
            Nakajima R, Arai E, Ishikawa M, Imaizumi K, Watanabe H, Okahara J, 
            Noce T, Takeda Y, Sasaki E, Behr R, Edamura K, Shiozawa S, Okano H
  TITLE     Non-viral Induction of Transgene-free iPSCs from Somatic Fibroblasts
            of Multiple Mammalian Species.
  JOURNAL   Stem Cell Reports. 2021 Mar 23. pii: S2213-6711(21)00132-6. doi: 
            10.1016/j.stemcr.2021.03.002.
  PUBMED    33798453
REFERENCE   2  (bases 1 to 12790)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Stem Cell 
            Reports. 2021 Mar 23. pii: S2213-6711(21)00132-6. doi: 
            10.1016/j.stemcr.2021.03.002."
COMMENT     Sequence Label: pCE-KdGl
FEATURES             Location/Qualifiers
     source          1..12790
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        382..659
                     /label=chicken beta-actin promoter
     intron          660..1676
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1600..1622
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1684..1703
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      1731..1740
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3306..3359
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     primer_bind     complement(5234..5253)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    5299..5354
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(5353..5372)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5715..5731)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5715..5731)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5728..5750)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5739..5755
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5763..5769,5770..5787,5788..5793))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5808..5829
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     CDS             6308..8233
                     /codon_start=1
                     /product="Epstein-Barr nuclear antigen 1, also known as 
                     EBNA-1"
                     /label=EBNA1
                     /note="crucial for latent viral infection, and for episomal
                     amplification of vectors containing the oriP origin (Okita 
                     et al., 2013)"
                     /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
                     GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
                     AGGGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGAGGAGAGGGGGGGGGXGGAGAGGGA
                     GGAGGAGAGGGAGAGGGAGGAGAGGGAGGAGGGGXGGGAGAGGGGGGGGAGAGGAGAGG
                     GAGGAGGAGAGGAGAGGAGXGGAGAGGGGGAGGGGAGGAGAGGAGGAGAGGGAGGAGAG
                     GGGGGGGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG
                     GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP
                     PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR
                     RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL
                     YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK
                     DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE
                     GEEGQE"
     rep_origin      8535..10324
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     primer_bind     complement(10865..10882)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11036..11624)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11116..11135)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11795..12586,12587..12655))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12418..12437
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12656..12760)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtcgaggtga gccccacgtt ctgcttcact ctccccatct
      421 cccccccctc cccaccccca attttgtatt tatttatttt ttaattattt tgtgcagcga
      481 tgggggcggg gggggggggg gggcgcgcgc cggggggggg gggggggggg gggggggggg
      541 ggggggggag gcggagaggt gcggcggcag ccaatcagag cggcgcgctc cgaaagtttc
      601 cttttatggc gaggcggcgg cggcggcggc cctataaaaa gcgaagcgcg cggcgggcgg
      661 gagtcgctgc gcgctgcctt cgccccgtgc cccgctccgc cgccgcctcg cgccgcccgc
      721 cccggctctg actgaccgcg ttactcccac aggtgagcgg gcgggacggc ccttctcctc
      781 cgggctgtaa ttagcgcttg gtttaatgac ggcttgtttc ttttctgtgg ctgcgtgaaa
      841 gccttgaggg gctccgggag ggccctttgt gcggggggag cggctcgggg ggtgcgtgcg
      901 tgtgtgtgtg cgtggggagc gccgcgtgcg gctccgcgct gcccggcggc tgtgagcgct
      961 gcgggcgcgg cgcggggctt tgtgcgctcc gcagtgtgcg cgaggggagc gcggccgggg
     1021 gcggtgcccc gcggtgcggg gggggctgcg aggggaacaa aggctgcgtg cggggtgtgt
     1081 gcgtgggggg gtgagcaggg ggtgtgggcg cgtcggtcgg gctgcaaccc cccctgcacc
     1141 cccctccccg agttgctgag cacggcccgg cttcgggtgc ggggctccgt acggggcgtg
     1201 gcgcggggct cgccgtgccg ggcggggggt ggcggcaggt gggggtgccg ggcggggcgg
     1261 ggccgcctcg ggccggggag ggctcggggg aggggcgcgg cggcccccgg agcgccggcg
     1321 gctgtcgagg cgcggcgagc cgcagccatt gccttttatg gtaatcgtgc gagagggcgc
     1381 agggacttcc tttgtcccaa atctgtgcgg agccgaaatc tgggaggcgc cgccgcaccc
     1441 cctctagcgg gcgcggggcg aagcggtgcg gcgccggcag gaaggaaatg ggcggggagg
     1501 gccttcgtgc gtcgccgcgc cgccgtcccc ttctccctct ccagcctcgg ggctgtccgc
     1561 ggggggacgg ctgccttcgg gggggacggg gcagggcggg gttcggcttc tggcgtgtga
     1621 ccggcggctc tagagcctct gctaaccatg ttcatgcctt cttctttttc ctacagctcc
     1681 tgggcaacgt gctggttatt gtgctgtctc atcattttgg caaagaattc gccaccatgg
     1741 aaagtatgaa gtctaaggcc aactgtgccc agaatccaaa ctgtaacata atgatatttt
     1801 atccaaccaa agaagagttt aatgattttg ataaatatat tgcttacatg gaatcccaag
     1861 gtgcacatag agctggcttg gctaagataa ttccaccgaa agaatggaaa gccagagaga
     1921 cctatgataa tatcagtgaa atcttaatag ccactcccct ccagcagatg gcctctgggc
     1981 gggcaggggt gtttactcaa taccataaaa agaagaaagc catgaccgtc ggggagtatc
     2041 gccatttggc aaatagtgaa aaatatcgga ctccaccaca cctgaatttc gaagatttgg
     2101 agcgaaaata ctggaagaac cgcatctata attcaccaat ttatggtgct gacatcagtg
     2161 gctccttgtt tgatgaaaac actaaacaat ggaatcttgg gcacctggga acaattcagg
     2221 acctgctgga gcaggaatgt ggggttgtca tagaaggcgt caatacaccc tacttgtact
     2281 ttggcatgtg gaagaccacg ttcgcttggc acacggagga catggacctt tacagcatca
     2341 actacctgca ccttggggag cccaaaactt ggtacgcagt gcccccagaa catggccagc
     2401 gcctggaacg cctggccagg gagctctttc caggcagttc ccgaggctgt gaggccttcc
     2461 tgcgccacaa ggtagccctc atctcaccta cagttctcaa agagaacggg attcctttta
     2521 atcgcatgac tcaggaggct ggggagttca tggtgacttt tccctatggc tatcatgcag
     2581 gcttcaatca tggcttcaat tgtgcagagg ccatcaattt tgccactcca cgatggattg
     2641 attatggcaa agtggcctct cagtgtagct gtggggaggc gagggtgacc ttttccatgg
     2701 acgccttcgt acgcatcctg caacccgagc gctatgagct gtggaaacgt gggcaagacc
     2761 gggcagttgt ggaccacatg gagcccagag tactagccag ccaagagctg aacacccaga
     2821 aggaagtcga gttgcctagg agagcagagt tgggcctgag acaactctcg ccccactggg
     2881 cccggcattc ccctttgcca atggctgccc gcagtgggac tcgctcccac acccttgtgt
     2941 gctcttcact cccacgccga tctgcagtca gtggcactgc tacgcagccc agagcggctg
     3001 ctgtccacag ctctaagaag cctaggtcaa ctctatcatc cacccctggt ccatctgcac
     3061 agattatcca cccgtcaaat ggcagacgtg gtcgtggtcg ccctcctcag aaactaagag
     3121 ctcagcagct gactctccag attccagcca aaaggcccct cttgctgggt acaacatgca
     3181 cagctttggg cccagaacct gagcccctgc ctgaggatgg ggctttgatg gacaagcctg
     3241 taccactgag ccctgggctc cagcatcctg ttaaggcttc tgggtgcaga tggacttctg
     3301 tgcccgaggg caggggaagt cttctaacat gcggggacgt ggaggaaaat cccggcccca
     3361 tggcagaggc ccgcacatcc ctgtctgccc actgtcgggg cccgctggcc actggcctgc
     3421 acccagacct ggacctcccg ggccgaagcc tcgccacccc tgcgccttcc tgctaccttc
     3481 tgggcagcga acccagctct ggcctgggcc tccagcccga gacccacctc cccgagggca
     3541 gcctgaagcg gtgctgtgtc ttgggcctac cccccacctc cccagcctcc tcctcaccct
     3601 gtgcctcctc cgacgtcacc tccatcatcc gctcctccca gacgtctctg gtcacctgtg
     3661 taaatggact ccggagcccc cctctggcgg gagatctggg gggcccttcc aagcgggccc
     3721 ggcctggccc tgcatcgacg gacagccatg agggcagctt gcaacttgaa gcctgccgga
     3781 aggcgagctt cctgaagcag gaacccgcgg atgagttttc agagctcttt gggcctcacc
     3841 agcagggcct gccgcccccc tatcccctgt ctcagttgcc gcctggccca agccttggag
     3901 gcctggggct gggcctggga ggcagggtgg tggccgggcg gcaggcgtgc cgctgggtgg
     3961 actgctgtgc agcctatgag cagcaggagg agctggtgcg gcacatcgag aagagccaca
     4021 ccgaccagcg caagggcgag gacttcacct gcttctgggc tggctgcgtg cgccgctaca
     4081 agcccttcaa cgcccgctac aagctgctca tccacatgcg agtgcactcg ggcgagaagc
     4141 ccaacaagtg catgtttgaa ggctgcagca aggccttctc acggctggag aacctcaaga
     4201 tccacctgag gagccacacg ggcgagaagc cgtacctgtg ccagcacccg ggttgccaga
     4261 aggccttcag caactccagc gaccgcgcca agcaccagcg cacccaccta gacacgaagc
     4321 cgtacgcctg tcagatccct ggctgctcca agcgctacac agaccccagc tccctccgca
     4381 agcacgtcaa ggcccattca gccaaagagc agcaggtgcg taagaagctg catgcgggcc
     4441 ctgacaccga ggccgacgtc ctgaccgagt gtctggtcct gcagcagctc cacacgtcca
     4501 cacagctggc tgccagcgac ggcaagggtg gctgtggcct gggccaggag ctgctcccag
     4561 gtgtgtatcc tggctccatc accccccata acggacttgc atcgggcctc ctgcccccag
     4621 cgcacgacgt accttccagg caccacccgc tggatgccac caccagttcc caccaccatc
     4681 tgtcccctct gcccatggct gagagcaccc gggatgggtt ggggcccggc ctcctctcac
     4741 caatagtcag ccccctgaag gggctggggc caccgccgct gcccccatcc tctcagagcc
     4801 attctccggg gggccagccc ttccccacac tccccagcaa gccgtcctac ccacccttcc
     4861 agagccctcc acccccgcct ctgcccagcc cacaaggtta ccagggcagt ttccactcca
     4921 tccagagttg cttcccctat ggcgactgct accggatggc tgaaccagca gccggtgggg
     4981 acggactggt cggggagacc cacggtttca accccctgcg gcccaatggc taccacagcc
     5041 tcagcacgcc cttgcctgcc acaggctatg aggccctggc tgaggcctca tgccccacag
     5101 cgctgccaca gcagccatct gaagatgtgg tgtccagcgg ccccgaggac tgtggcttct
     5161 tccccaatgg agcctttgac cactgcctgg gccacatccc ctccatctac acagacacct
     5221 gagcggccgc ggatcttttt ccctctgcca aaaattatgg ggacatcatg aagccccttg
     5281 agcatctgac ttctggctaa taaaggaaat ttattttcat tgcaatagtg tgttggaatt
     5341 ttttgtgtct ctcactcgga aggacatatg ggagggcaaa tcatttaaaa catcagaatg
     5401 agtatttggt ttagagtttg gcaacatatg cccatatgct ggctgccatg aacaaaggtt
     5461 ggctataaag aggtcatcag tatatgaaac agccccctgc tgtccattcc ttattccata
     5521 gaaaagcctt gacttgaggt tagatttttt ttatattttg ttttgtgtta tttttttctt
     5581 taacatccct aaaattttcc ttacatgttt tactagccag atttttcctc ctctcctgac
     5641 tactcccagt catagctgtc cctcttctct tatggagatc cctcgacctg cagcccaagc
     5701 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
     5761 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
     5821 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
     5881 cggatctcaa ttctcatgtt tgacagctta tcatcgataa gctgatcctc acaggccgca
     5941 cccagctttt cttccgttgc cccagtagca tctctgtctg gtgaccttga agaggaagag
     6001 gaggggtccc gagaatcccc atccctaccg tccagcaaaa agggggacga ggaatttgag
     6061 gcctggcttg aggctcagga cgcaaatctt gaggatgttc agcgggagtt ttccgggctg
     6121 cgagtaattg gtgatgagga cgaggatggt tcggaggatg gggaattttc agacctggat
     6181 ctgtctgaca gcgaccatga aggggatgag ggtggggggg ctgttggagg gggcaggagt
     6241 ctgcactccc tgtattcact gagcgtcgtc taataaagat gtctattgat ctcttttagt
     6301 gtgaatcatg tctgacgagg ggccaggtac aggacctgga aatggcctag gagagaaggg
     6361 agacacatct ggaccagaag gctccggcgg cagtggacct caaagaagag ggggtgataa
     6421 ccatggacga ggacggggaa gaggacgagg acgaggaggc ggaagaccag gagccccggg
     6481 cggctcagga tcagggccaa gacatagaga tggtgtccgg agaccccaaa aacgtccaag
     6541 ttgcattggc tgcaaaggga cccacggtgg aacaggagca ggagcaggag cgggaggggc
     6601 aggagcagga ggggcaggag caggaggagg ggcaggagca ggaggagggg caggaggggc
     6661 aggaggggca ggaggggcag gagcaggagg aggggcagga gcaggaggag gggcaggagg
     6721 ggcaggaggg gcaggagcag gaggaggggg aggaggagga ggaggggsag gaggggcagg
     6781 agcaggagga ggggcaggag gggcaggagg ggcaggagca ggaggagggg caggagcagg
     6841 aggaggggca ggaggggcag gagcaggagg aggggcagga ggggcaggag ggggaggags
     6901 aggaggaggg gcaggagcag gagggggagg agggggagga ggggcaggag caggaggggc
     6961 aggagcagga ggaggggcag gaggggcagg aggggcagga gcaggagggg caggagcagg
     7021 aggggcagga gsaggagggg caggagcagg agggggagga ggggcaggag gaggaggggc
     7081 aggaggggca ggagcaggag gggcaggagg ggcaggagca ggaggagggg caggaggggc
     7141 aggagcagga ggagggggag gagggggagg agcaggaggg gcaggagggg caggagcagg
     7201 aggggcagga ggggcaggag caggaggggc aggaggggca ggagcaggag gaggggcagg
     7261 agcaggaggg gcaggagcag gaggtggagg ccggggtcga ggaggcagtg gaggccgggg
     7321 tcgaggaggt agtggaggcc ggggtcgagg aggtagtgga ggccgccggg gtagaggacg
     7381 tgaaagagcc agggggggaa gtcgtgaaag agccaggggg agaggtcgtg gacgtggaga
     7441 aaagaggccc aggagtccca gtagtcagtc atcatcatcc gggtctccac cgcgcaggcc
     7501 ccctccaggt agaaggccat ttttccaccc tgtaggggaa gccgattatt ttgaatacca
     7561 ccaagaaggt ggcccagatg gtgagcctga cgtgcccccg ggagcgatag agcagggccc
     7621 cgcagatgac ccaggagaag gcccaagcac tggaccccgg ggtcagggtg atggaggcag
     7681 gcgcaaaaaa ggagggtggt ttggaaagca tcgtggtcaa ggaggttcca acccgaaatt
     7741 tgagaacatt gcagaaggtt taagagctct cctggctagg agtcacgtag aaaggactac
     7801 cgacgaagga acttgggtcg ccggtgtgtt cgtatatgga ggtagtaaga cctcccttta
     7861 caacctaagg cgaggaactg cccttgctat tccacaatgt cgtcttacac cattgagtcg
     7921 tctccccttt ggaatggccc ctggacccgg cccacaacct ggcccgctaa gggagtccat
     7981 tgtctgttat ttcatggtct ttttacaaac tcatatattt gctgaggttt tgaaggatgc
     8041 gattaaggac cttgttatga caaagcccgc tcctacctgc aatatcaggg tgactgtgtg
     8101 cagctttgac gatggagtag atttgcctcc ctggtttcca cctatggtgg aaggggctgc
     8161 cgcggagggt gatgacggag atgacggaga tgaaggaggt gatggagatg agggtgagga
     8221 agggcaggag tgatgtaact tgttaggaga cgccctcaat cgtattaaaa gccgtgtatt
     8281 cccccgcact aaagaataaa tccccagtag acatcatgcg tgctgttggt gtatttctgg
     8341 ccatctgtct tgtcaccatt ttcgtcctcc caacatgggg caattgggca tacccatgtt
     8401 gtcacgtcac tcagctccgc gctcaacacc ttctcgcgtt ggaaaacatt agcgacattt
     8461 acctggtgag caatcagaca tgcgacggct ttagcctggc ctccttaaat tcacctaaga
     8521 atgggagcaa ccagcaggaa aaggacaagc agcgaaaatt cacgccccct tgggaggtgg
     8581 cggcatatgc aaaggatagc actcccactc tactactggg tatcatatgc tgactgtata
     8641 tgcatgagga tagcatatgc tacccggata cagattagga tagcatatac tacccagata
     8701 tagattagga tagcatatgc tacccagata tagattagga tagcctatgc tacccagata
     8761 taaattagga tagcatatac tacccagata tagattagga tagcatatgc tacccagata
     8821 tagattagga tagcctatgc tacccagata tagattagga tagcatatgc tacccagata
     8881 tagattagga tagcatatgc tatccagata tttgggtagt atatgctacc cagatataaa
     8941 ttaggatagc atatactacc ctaatctcta ttaggatagc atatgctacc cggatacaga
     9001 ttaggatagc atatactacc cagatataga ttaggatagc atatgctacc cagatataga
     9061 ttaggatagc ctatgctacc cagatataaa ttaggatagc atatactacc cagatataga
     9121 ttaggatagc atatgctacc cagatataga ttaggatagc ctatgctacc cagatataga
     9181 ttaggatagc atatgctatc cagatatttg ggtagtatat gctacccatg gcaacattag
     9241 cccaccgtgc tctcagcgac ctcgtgaata tgaggaccaa caaccctgtg cttggcgctc
     9301 aggcgcaagt gtgtgtaatt tgtcctccag atcgcagcaa tcgcgcccct atcttggccc
     9361 gcccacctac ttatgcaggt attccccggg gtgccattag tggttttgtg ggcaagtggt
     9421 ttgaccgcag tggttagcgg ggttacaatc agccaagtta ttacaccctt attttacagt
     9481 ccaaaaccgc agggcggcgt gtgggggctg acgcgtgccc ccactccaca atttcaaaaa
     9541 aaagagtggc cacttgtctt tgtttatggg ccccattggc gtggagcccc gtttaatttt
     9601 cgggggtgtt agagacaacc agtggagtcc gctgctgtcg gcgtccactc tctttcccct
     9661 tgttacaaat agagtgtaac aacatggttc acctgtcttg gtccctgcct gggacacatc
     9721 ttaataaccc cagtatcata ttgcactagg attatgtgtt gcccatagcc ataaattcgt
     9781 gtgagatgga catccagtct ttacggcttg tccccacccc atggatttct attgttaaag
     9841 atattcagaa tgtttcattc ctacactagt atttattgcc caaggggttt gtgagggtta
     9901 tattggtgtc atagcacaat gccaccactg aaccccccgt ccaaatttta ttctgggggc
     9961 gtcacctgaa accttgtttt cgagcacctc acatacacct tactgttcac aactcagcag
    10021 ttattctatt agctaaacga aggagaatga agaagcaggc gaagattcag gagagttcac
    10081 tgcccgctcc ttgatcttca gccactgccc ttgtgactaa aatggttcac taccctcgtg
    10141 gaatcctgac cccatgtaaa taaaaccgtg acagctcatg gggtgggaga tatcgctgtt
    10201 ccttaggacc cttttactaa ccctaattcg atagcatatg cttcccgttg ggtaacatat
    10261 gctattgaat tagggttagt ctggatagta tatactacta cccgggaagc atatgctacc
    10321 cgtttagggt taacaagggg gccttataaa cactattgct aatgccctct tgagggtccg
    10381 cttatcggta gctacacagg cccctctgat tgacgttggt gtagcctccc gtagtcttcc
    10441 tgggcccctg ggaggtacat gtcccccagc attggtgtaa gagcttcagc caagagttac
    10501 acataaaggc aatgttgtgt tgcagtccac agactgcaaa gtctgctcca ggatgaaagc
    10561 cactcagtgt tggcaaatgt gcacatccat ttataaggat gtcaactaca gtcagagaac
    10621 ccctttgtgt ttggtccccc cccgtgtcac atgtggaaca gggcccagtt ggcaagttgt
    10681 accaaccaac tgaagggatt acatgcactg ccccgcgaag aaggggcaga gatgtcgtag
    10741 tcaggtttag ttcgtccggg gcggggatcg atcctctaga gtcgacgcta gcggatccgc
    10801 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
    10861 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
    10921 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
    10981 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
    11041 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
    11101 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
    11161 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
    11221 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
    11281 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
    11341 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
    11401 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
    11461 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
    11521 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
    11581 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
    11641 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
    11701 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
    11761 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
    11821 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
    11881 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
    11941 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
    12001 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
    12061 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
    12121 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
    12181 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
    12241 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
    12301 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
    12361 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
    12421 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
    12481 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
    12541 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
    12601 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
    12661 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
    12721 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
    12781 cacctgggtc
//
