LOCUS       sequence-313246-        5511 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression plasmid for SARS-CoV-2 N (nucleocapsid).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5511)
  TITLE     SARS-CoV-2 protein Mammalian expression constructs, tagged, for 
            protein expression and/or purification
REFERENCE   2  (bases 1 to 5511)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGBW-m4134546
FEATURES             Location/Qualifiers
     source          1..5511
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        306..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             1840..1860
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             1861..1905
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     polyA_signal    1958..2079
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1969..1988)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2023..2042
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     terminator      2194..2240
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      2270..2406
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    2451..2522
                     /label=3' beta-globin insulator
                     /note="insulator downstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     rep_origin      complement(2748..3336)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2828..2847)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             join(4082..4150,4151..4942)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4300..4319)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     terminator      4955..5091
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    5243..5314
                     /label=5' beta-globin insulator
                     /note="insulator upstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     terminator      complement(5319..5365)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     polyA_signal    5383..5431
                     /note="synthetic polyadenylation signal"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catgctgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatca gatctttgtc
      541 gatcctacca tccactcgac acacccgcca gcggccgcca ccatgtctga taatggacca
      601 cagaaccaac gcaatgcacc ccgaatcact tttggcggtc cgtccgacag taccgggagc
      661 aaccagaacg gcgaaagatc gggagctcgt tcaaaacagc ggaggcctca agggttaccc
      721 aataacacgg ccagctggtt cacagcgttg acccagcatg gcaaggagga cctcaagttc
      781 cctcggggtc agggggtccc aataaataca aacagttccc ctgatgacca aattggatac
      841 tatagacgcg ctactaggag gatccgtggc ggggatggaa aaatgaagga cctttctccc
      901 agatggtact tttattacct gggtactggc ccagaggccg gtctaccgta tggagcaaac
      961 aaagatggca ttatttgggt ggccacggaa ggagcactga ataccccaaa ggatcacatc
     1021 ggcacccgga atccagccaa caatgctgct atcgttctgc aactccccca ggggacaaca
     1081 ttgcctaaag gcttctatgc cgagggcagt agaggtggca gccaggcgtc ctcacgcagc
     1141 tcttctcgat ctcggaacag ctcaagaaat tccactcccg ggagttcccg aggaaccagc
     1201 ccagcaagga tggctggcaa tggtggagat gccgctctgg ccctacttct gctggaccgc
     1261 ctcaatcagt tagaatcaaa gatgtcgggg aaggggcagc aacagcaggg acagaccgta
     1321 actaaaaaga gtgcagcgga ggcctctaaa aagcctcggc agaaacgtac ggcaacaaag
     1381 gcttacaacg tgacccaggc ctttggccgt agaggacccg aacagacaca agggaacttt
     1441 ggtgaccaag agttgataag gcagggaacc gactacaaac actggcctca gatcgcccaa
     1501 ttcgcaccga gcgcgagcgc atttttcgga atgtcacgaa ttggcatgga ggtgacacct
     1561 tcgggaacct ggctgacata cactggcgcc ataaagctcg atgacaaaga tcccaacttc
     1621 aaggatcaag tcatcttgct gaataagcat attgacgcgt ataaaacttt tccacccacg
     1681 gaaccgaaga aggacaagaa aaagaaagct gatgagacac aggcgcttcc acaaaggcag
     1741 aaaaagcagc agacggttac tctactgccc gccgcagacc tagatgactt ctccaaacag
     1801 cttcagcagt ctatgagtag cgccgactca acccaagctg aaaaccttta ctttcagtcg
     1861 aaagaaaccg cggcagctaa gttcgagagg cagcacatgg acagttaagc tagcttgact
     1921 gactgagata cagcgtacct tcagctcaca gacatgataa gatacattga tgagtttgga
     1981 caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt
     2041 gctttatttg taaccattat aagctgcaat aaacaagtta acaacaacaa ttgcattcat
     2101 tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt aaagcaagta aaacctctac
     2161 aaatgtggta ttggcccatc tctatcggta tcgtagcata accccttggg gcctctaaac
     2221 gggtcttgag gggttttttg tgcccctcgg gccggattgc tatctaccgg cattggcgca
     2281 gaaaaaaatg cctgatgcga cgctgcgcgt cttatactcc cacatatgcc agattcagca
     2341 acggatacgg cttccccaac ttgcccactt ccatacgtgt cctccttacc agaaatttat
     2401 ccttaaggtc gtcagctatc ctgcaggcga tctctcgatt tcgatcaaga cattccttta
     2461 atggtctttt ctggacacca ctaggggtca gaagtagttc atcaaacttt cttccctccc
     2521 taatctcatt ggttaccttg ggctatcgaa acttaattaa ccagtcaagt cagctacttg
     2581 gcgagatcga cttgtctggg tttcgactac gctcagaatt gcgtcagtca agttcgatct
     2641 ggtccttgct attgcacccg ttctccgatt acgagtttca tttaaatcat gtgagcaaaa
     2701 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     2761 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     2821 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     2881 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     2941 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     3001 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     3061 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     3121 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     3181 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     3241 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     3301 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     3361 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     3421 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     3481 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     3541 gcgatctgtc tatttcgttc atccatagtt gcatttaaat ttccgaactc tccaaggccc
     3601 tcgtcggaaa atcttcaaac ctttcgtccg atccatcttg caggctacct ctcgaacgaa
     3661 ctatcgcaag tctcttggcc ggccttgcgc cttggctatt gcttggcagc gcctatcgcc
     3721 aggtattact ccaatcccga atatccgaga tcgggatcac ccgagagaag ttcaacctac
     3781 atcctcaatc ccgatctatc cgagatccga ggaatatcga aatcggggcg cgcctggtgt
     3841 accgagaacg atcctctcag tgcgagtctc gacgatccat atcgttgctt ggcagtcagc
     3901 cagtcggaat ccagcttggg acccaggaag tccaatcgtc agatattgta ctcaagcctg
     3961 gtcacggcag cgtaccgatc tgtttaaacc tagatattga tagtctgatc ggtcaacgta
     4021 taatcgagtc ctagcttttg caaacatcta tcaagagaca ggatcagcag gaggctttcg
     4081 catgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
     4141 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
     4201 gcgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
     4261 cgaagaacgc tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
     4321 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
     4381 ggttgagtat tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
     4441 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     4501 tggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
     4561 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     4621 gcctgtagca atggcaacaa ccttgcgtaa actattaact ggcgaactac ttactctagc
     4681 ttcccggcaa cagttgatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     4741 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     4801 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     4861 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     4921 ctcactgatt aagcattggt aaccgattct aggtgcattg gcgcagaaaa aaatgcctga
     4981 tgcgacgctg cgcgtcttat actcccacat atgccagatt cagcaacgga tacggcttcc
     5041 ccaacttgcc cacttccata cgtgtcctcc ttaccagaaa tttatcctta agatcccgaa
     5101 tcgtttaaac tcgactctgg ctctatcgaa tctccgtcgt ttcgagctta cgcgaacagc
     5161 cgtggcgctc atttgctcgt cgggcatcga atctcgtcag ctatcgtcag cttacctttt
     5221 tggcagcgat cgcggctccc gacatcttgg accattagct ccacaggtat cttcttccct
     5281 ctagtggtca taacagcagc ttcagctacc tctcaattca aaaaacccct caagacccgt
     5341 ttagaggccc caaggggtta tgctatcaat cgttgcgtta cacacacaaa aaaccaacac
     5401 acatccatct tcgatggata gcgattttat tatctaactg ctgatcgagt gtagccagat
     5461 ctagtaatca attacggggt cattagttca tagcccatat atggagttcc g
//
