LOCUS       sequence-31325-e         871 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 871)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 871)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: CBX1
FEATURES             Location/Qualifiers
     source          1..871
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             37..54
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             67..87
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             279..296
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(349..367)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      363..410
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      447..827
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(534..553)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     744..765
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 aatattttgt ttactttaag aaggagatat accatgcatc atcatcatca tcacagcagc
       61 ggcagagaaa acttgtattt ccagggcgaa tatgtggtgg aaaaagttct cgaccgtcga
      121 gtggtaaagg gcaaagtgga gtacctccta aagtggaagg gattctcaga tgaggacaac
      181 acatgggagc cagaagagaa cctggattgc cccgacctca ttgctgagtt tctgcagtca
      241 cagaaaacat gatgacgaag cttgcggccg cactcgagca ccaccaccac caccactgag
      301 atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat
      361 aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag
      421 gaactatatc cggattggcg aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg
      481 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
      541 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
      601 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
      661 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
      721 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
      781 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
      841 aaatgagctg atttaacaaa aatttaacgc g
//
