LOCUS       sequence-313254-        1168 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of ""extended"" membrane scaffold protein 
            MSP1E2D1 for Nanodisc formation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1168)
  AUTHORS   Ritchie TK, Grinkova YV, Bayburt TH, Denisov IG, Zolnerciks JK, 
            Atkins WM, Sligar SG
  TITLE     Chapter 11 - Reconstitution of membrane proteins in phospholipid 
            bilayer nanodiscs.
  JOURNAL   Methods Enzymol. 2009;464:211-31. doi: 
            10.1016/S0076-6879(09)64011-8.
  PUBMED    19903557
REFERENCE   2  (bases 1 to 1168)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods 
            Enzymol."; date: "2009"; volume: "464:211-31. doi"; pages: " 
            10.1016/S0076-6879(09)64011-8"
COMMENT     Sequence Label: pMSP1E2D1
FEATURES             Location/Qualifiers
     source          1..1168
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             26..48
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             61..81
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=7xHis
                     /translation="HHHHHHH"
     CDS             103..123
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             848..865
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(918..936)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      932..979
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(1103..1122)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ttagggaaat tccctctaga ataattttgt ttaactttaa gaaggagata taccatgggt
       61 catcatcatc atcatcatca cgattatgat attcctacta ctgagaattt gtattttcag
      121 ggttctacct tcagtaaact tcgcgaacaa ctgggccccg tgacgcagga attctgggac
      181 aacctggaaa aagaaaccga gggactgcgt caggaaatgt ccaaagattt agaagaggtg
      241 aaggccaagg ttcagccata tctcgatgac tttcagaaaa aatggcagga agagatggaa
      301 ttatatcgtc aaaaggtgga accgctgcgt gcggaactgc aagagggggc acgccaaaaa
      361 ctccatgagc tccaagagaa gctcagccca tatctcgatg actttcagaa aaaatggcag
      421 gaagagatgg aattatatcg tcaaaaggtg gaaccgctgc gtgcggaact gcaagagggg
      481 gcacgccaaa aactccatga gctccaagag aagctcagcc cattaggcga agaaatgcgc
      541 gatcgcgccc gtgcacatgt tgatgcactc cggactcatt tggcgccgta ttcggatgaa
      601 cttcgccagc gtttggccgc acgtctcgag gcgctgaaag aaaacggggg tgcccgcttg
      661 gctgagtacc acgcgaaagc gacagaacac ctgagcacct tgagcgaaaa agcgaaaccg
      721 gcgctggaag atctacgcca gggcttattg cctgttcttg agagctttaa agtcagtttt
      781 ctgtcagctc tggaagaata tactaaaaag ctgaataccc agtaataagc ttgcggccgc
      841 actcgagcac caccaccacc accactgaga tccggctgct aacaaagccc gaaaggaagc
      901 tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg
      961 ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggattggcga atgggacgcg
     1021 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     1081 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     1141 gccggctttc ccgtcaagct ctaatcgg
//
