LOCUS       sequence-313256-        1181 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of mutated membrane scaffold protein 
            (""ultra-dark"" MSP1D1) for Nanodisc formation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1181)
  AUTHORS   McLean MA, Denisov IG, Grinkova YV, Sligar SG
  TITLE     Dark, Ultra-Dark and Ultra-Bright Nanodiscs for membrane protein 
            investigations.
  JOURNAL   Anal Biochem. 2020 Oct 15;607:113860. doi: 10.1016/j.ab.2020.113860.
            Epub 2020 Aug 1.
  PUBMED    32750355
REFERENCE   2  (bases 1 to 1181)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Anal 
            Biochem."; date: "2020-10-15"; volume: "607:113860. doi"; pages: " 
            10.1016/j.ab.2020.113860. Epub 2020 Aug 1"
COMMENT     Sequence Label: pUD-MSP1D1
FEATURES             Location/Qualifiers
     source          1..1181
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             27..49
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             62..82
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=7xHis
                     /translation="HHHHHHH"
     CDS             104..124
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             713..730
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(783..801)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      797..844
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(969..988)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 tttgaggaaa ttccctctag aataattttg tttaacttta agaaggagat ataccatggg
       61 tcatcatcat catcatcatc acgattatga tattcctact actgagaatt tgtattttca
      121 gggttctacc ttcagtaaac ttcgcgaaca actgggcccc gtgacgcagg aattcttcga
      181 caacctggaa aaagaaaccg agggactgcg tcaggaaatg tccaaagatt tagaagaggt
      241 gaaggccaag gttcagccat ttctcgatga ctttcagaaa aaattccagg aagagatgga
      301 attatttcgt caaaaggtgg aaccgctgcg tgcggaactg caagaggggg cacgccaaaa
      361 actccatgag ctccaagaga agctcagccc attaggcgaa gaaatgcgcg atcgcgcccg
      421 tgcacatgtt gatgcactcc ggactcattt ggcgccgttt tcggatgaac ttcgccagcg
      481 tttggccgca cgtctggagg cgctgaaaga aaacgggggt gcccgcttgg ctgagttcca
      541 cgcgaaagcg acagaacacc tgagcacctt gagcgaaaaa gcgaaaccgg cgctggaaga
      601 tctacgccag ggcttattgc ctgttcttga gagctttaaa gtcagttttc tgtcagctct
      661 ggaagaattt actaaaaagc tgaataccca gtaagcttgc ggccgactcg agcaccacca
      721 ccaccaccac tgagatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc
      781 caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt
      841 tttgctgaaa ggaggaacta tatccggatt ggcgaatggg acgcgccctg tagcggcgca
      901 ttaagcgcgg cggggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
      961 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
     1021 tcagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
     1081 cccaaaaact tgaattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
     1141 ttcgcccttt gacgttggag tcccgttctt taatagtgga c
//
