LOCUS       sequence-313260-       13418 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Baculovirus protein expression of His6-tev-Hs.NF1opt (1-2818) 
            isoform 2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13418)
  AUTHORS   Sherekar M, Han SW, Ghirlando R, Messing S, Drew M, Rabara D, 
            Waybright T, Juneja P, O'Neill H, Stanley CB, Bhowmik D, Ramanathan 
            A, Subramaniam S, Nissley DV, Gillette W, McCormick F, Esposito D
  TITLE     Biochemical and structural analyses reveal that the tumor suppressor
            neurofibromin (NF1) forms a high-affinity dimer.
  JOURNAL   J Biol Chem. 2020 Jan 24;295(4):1105-1119. doi: 
            10.1074/jbc.RA119.010934. Epub 2019 Dec 13.
  PUBMED    31836666
REFERENCE   2  (bases 1 to 13418)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.RA119.010934"; journalName: "J Biol Chem"; date: 
            "2020-01-24- 24"; volume: "295"; issue: "4"; pages: "1105-1119"
COMMENT     Sequence Label: R702-X80-635: His6-tev-Hs.NF1opt (1-2818) isof...
FEATURES             Location/Qualifiers
     source          1..13418
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..92
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     17..34
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             152..169
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     protein_bind    182..206
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             206..226
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     protein_bind    complement(8679..8703)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    8861..8995
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8898..8917)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8952..8971
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  9024..9189
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      9373..9827
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9460..9479)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9669..9690
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9853..9957
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9958..10026,10027..10818)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10176..10195)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10989..11577
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11478..11497
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     mobile_element  11882..12106
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(12173..12706)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(12895..12900,12901..12917,12918..12923))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     promoter        complement(13286..13395)
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
ORIGIN
        1 atcatggaga taattaaaat gataaccatc tcgcaaataa ataagtattt tactgttttc
       61 gtaacagttt tgtaataaaa aaacctataa atattccgga ttattcatac cgtcccacca
      121 tcgggcgcgg atcaccatgg gatctggatc ccaccatcac catcaccata gatcctcgat
      181 cacaagtttg tacaaaaaag ttggcgaaaa cctgtacttc caaggcgccg cccacagacc
      241 tgtggaatgg gtgcaggccg tggtgtccag attcgacgag cagctgccca tcaagaccgg
      301 acagcagaat acacacacca aggtgtccac cgagcacaac aaagagtgcc tgatcaacat
      361 cagcaagtac aagttcagcc tggtgatcag cggcctgacc accatcctga agaacgtgaa
      421 caacatgcgg atcttcggcg aggccgccga gaagaacctg tacctgagcc agctgatcat
      481 cctggacacc ctggaaaagt gcctcgctgg acagcccaag gataccatgc ggctggacga
      541 gacaatgctg gtgaagcagc tgctgcccga gatctgccac ttcctgcaca cctgccggga
      601 gggaaatcag cacgccgccg agctgagaaa tagcgccagc ggcgtgctgt tcagcctgag
      661 ctgcaacaac ttcaacgccg tgttcagccg gatcagcacc cggctgcagg aactgaccgt
      721 ctgcagcgag gacaacgtgg acgtgcacga catcgagctg ctgcagtaca tcaacgtgga
      781 ctgcgccaag ctgaagcggc tgctgaaaga gacagccttc aagttcaagg ccctgaagaa
      841 ggtggcccag ctggccgtga tcaacagcct ggaaaaagcc ttctggaact gggtggagaa
      901 ctaccccgac gagttcacca agctgtacca gatcccccag accgacatgg ccgagtgcgc
      961 cgagaagctg ttcgacctgg tggacggctt cgccgagagc accaagagaa aggccgccgt
     1021 gtggcccctg cagatcatcc tgctgatcct gtgccccgag atcatccagg acatctccaa
     1081 ggacgtggtg gacgagaaca acatgaacaa gaagctgttt ctggacagcc tgaggaaggc
     1141 cctggccgga cacggcggct ctagacagct gaccgagagc gccgccattg cctgcgtgaa
     1201 gctgtgcaag gccagcacct acatcaactg ggaggacaac agcgtgatct tcctgctggt
     1261 gcagagcatg gtggtggacc tgaagaacct gctgttcaac cccagcaagc ccttcagcag
     1321 aggcagccag cccgccgacg tggacctgat gatcgactgc ctggtgtcct gcttccggat
     1381 cagcccccac aacaaccagc acttcaagat ctgcctggcc cagaacagcc ccagcacctt
     1441 ccactacgtg ctggtgaact ccctgcaccg gatcatcacc aacagcgccc tggactggtg
     1501 gcccaagatc gacgccgtgt actgccacag cgtggagctg cggaacatgt tcggcgagac
     1561 actgcacaag gccgtgcagg gatgtggagc ccaccccgcc atcagaatgg cccccagcct
     1621 gaccttcaaa gaaaaggtga cctccctgaa gttcaaagag aagcccaccg acctggaaac
     1681 ccggtcctac aagtacctgc tgctgtccat ggtgaagctg atccacgccg accccaagct
     1741 gctgctgtgc aaccctagga agcagggccc tgagacacag ggcagcacag ccgagctgat
     1801 caccggcctg gtgcagctgg tgccccagag ccacatgcct gagatcgccc aggaagccat
     1861 ggaagctctg ctcgtcctgc accagctcga ctccattgat ctgtggaacc ccgacgcccc
     1921 cgtggagaca ttctgggaga tcagcagcca gatgctgttc tacatctgca agaagctgac
     1981 cagccatcag atgctgtcca gcaccgagat cctgaagtgg ctgcgggaga tcctgatctg
     2041 ccggaacaag ttcctgctga agaacaagca ggccgaccgg tccagctgcc actttctgct
     2101 gttttacggc gtgggctgcg acatccccag cagcggcaac accagccaga tgagcatgga
     2161 ccacgaggaa ctgctgagaa cccctggcgc cagcctgaga aagggcaagg gcaacagcag
     2221 catggacagc gccgctggct gtagcggcac cccccctatc tgtcggcagg cccagacaaa
     2281 actggaagtc gctctgtaca tgttcctgtg gaatcccgat accgaggccg tgctggtcgc
     2341 catgagctgc ttccggcacc tgtgcgagga agccgacatc agatgcggcg tggatgaggt
     2401 gtccgtgcac aacctgctgc ccaactacaa caccttcatg gaattcgcca gcgtgagcaa
     2461 catgatgagc accggcagag ccgccctgca gaaacgggtg atggccctgc tgcggagaat
     2521 cgagcacccc accgccggaa atacagaggc ctgggaggat acccacgcca agtgggagca
     2581 ggccaccaag ctgatcctga actaccccaa ggccaagatg gaagatggcc aggccgccga
     2641 aagcctgcac aagaccatcg tgaagcggcg gatgagccac gtgtctggcg gcggaagcat
     2701 cgacctgagc gacaccgact ccctgcaaga atggatcaac atgaccggct ttctgtgtgc
     2761 cctgggcgga gtgtgcctgc agcagcggag caattccggc ctggccacct acagccctcc
     2821 tatgggcccc gtgagcgaga ggaagggcag catgatcagc gtgatgtcca gcgagggcaa
     2881 cgccgacacc cccgtgagca agttcatgga ccggctgctg tccctgatgg tctgcaacca
     2941 cgagaaagtg ggcctgcaga tccggaccaa cgtgaaggac ctggtcggcc tggaactgag
     3001 ccctgccctg taccccatgc tgttcaataa gctgaagaat accatctcca agttcttcga
     3061 cagccagggc caggtgctgc tgaccgacac caacacccag ttcgtggagc agacaatcgc
     3121 catcatgaag aatctgctgg acaaccacac cgagggcagc tctgaacacc tgggccaggc
     3181 cagcatcgag acaatgatgc tgaacctggt ccgctacgtg cgggtgctgg gcaacatggt
     3241 gcacgccatc cagatcaaga caaagctgtg ccagctggtc gaggtgatga tggccagacg
     3301 ggacgacctg agcttctgcc aggaaatgaa gttccggaac aagatggtgg agtacctgac
     3361 cgactgggtg atgggcacca gcaaccaggc cgctgacgac gacgtgaagt gcctgaccag
     3421 ggacctggac caggcttcta tggaagctgt cgtcagtctg ctggccggac tgcctctgca
     3481 gcctgaagaa ggtgacggcg tcgagctgat ggaagccaag agccagctgt tcctgaagta
     3541 cttcaccctg ttcatgaacc tgctgaacga ctgcagcgag gtggaggatg agagcgccca
     3601 gaccggcggc agaaagcggg gcatgagcag aaggctggcc tccctgaggc actgtaccgt
     3661 gctggccatg tccaatctgc tgaacgccaa cgtggatagc ggcctgatgc acagcatcgg
     3721 cctgggctac cacaaggacc tgcagacccg ggccaccttt atggaagtgc tgaccaagat
     3781 cctgcagcag ggcaccgagt tcgacaccct ggccgagact gtgctggccg acagattcga
     3841 gagactggtg gaactggtga ccatgatggg cgaccagggc gagctgccta ttgccatggc
     3901 cctggccaac gtggtgccct gtagccagtg ggatgagctg gccagggtcc tcgtcacact
     3961 gttcgacagc cggcacctgc tgtaccagct gctgtggaat atgttcagca aagaggtgga
     4021 gctggccgac tccatgcaga ccctgttcag aggcaactcc ctggccagca agatcatgac
     4081 cttttgcttc aaggtgtacg gcgccaccta cctgcagaag ctgctggacc ccctgctgag
     4141 gatcgtgatc accagcagcg actggcagca cgtgagcttc gaggtggacc ccaccagact
     4201 ggaacccagc gagagcctgg aagagaacca gcggaacctg ctgcagatga ccgagaagtt
     4261 cttccacgcc atcatcagca gcagctccga gttccccccc cagctgagaa gcgtgtgcca
     4321 ctgcctgtac caggtggtgt cccagcggtt cccccagaat agcatcggcg ccgtgggcag
     4381 cgccatgttc ctgcggttca tcaaccctgc catcgtgagc ccttacgagg ccggcatcct
     4441 ggataagaag ccccctcccc ggatcgagag gggcctgaag ctgatgagca agattctgca
     4501 gagcattgcc aaccacgtgc tgtttaccaa ggaagagcac atgcggccct tcaacgactt
     4561 cgtgaagagc aacttcgacg ccgccaggcg gttcttcctg gatatcgcca gcgactgccc
     4621 taccagcgac gccgtgaacc acagcctgtc cttcatcagc gacggcaacg tcctggccct
     4681 gcatagactg ctgtggaaca accaggaaaa gatcggccag tacctgagca gcaaccggga
     4741 ccacaaggct gtgggcaggc ggcccttcga taagatggcc accctgctgg cctatctggg
     4801 ccctcccgag cacaagcctg tggccgacac ccattggtct agcctgaacc tgaccagcag
     4861 caagttcgag gaattcatga cccggcacca ggtgcacgag aaagaagagt ttaaagccct
     4921 gaaaaccctg agcatcttct accaggccgg cacctccaag gccggcaacc ccatcttcta
     4981 ctacgtggcc agaaggttca agacaggcca gatcaacggc gacctgctga tctaccacgt
     5041 gctgctgaca ctgaagccct actacgccaa gccctacgag atcgtggtgg atctgaccca
     5101 caccggcccc agcaaccggt tcaagaccga cttcctgagc aagtggttcg tggtgttccc
     5161 cggcttcgcc tacgataacg tgagcgccgt gtacatctac aactgcaaca gctgggtgcg
     5221 ggagtacacc aagtaccacg agcgcctgct gaccggcctg aagggctcca agcggctggt
     5281 gttcatcgac tgccccggca agctggccga gcacatcgag cacgagcagc agaaactgcc
     5341 tgccgccacc ctggccctgg aagaggacct gaaggtgttc cacaacgccc tgaagctggc
     5401 ccacaaggat acaaaggtgt ccatcaaagt gggcagcacc gccgtgcagg tgaccagcgc
     5461 cgagaggaca aaggtgctgg gccagagcgt gttcctgaac gacatctact acgcctccga
     5521 gatcgaggaa atctgcctgg tggatgagaa ccagttcacc ctgactatcg ccaaccaggg
     5581 cacccctctg accttcatgc accaggaatg cgaggccatc gtccagtcca tcatccacat
     5641 ccggaccaga tgggagctgt cccagcccga cagcatcccc cagcacacca agatccggcc
     5701 caaggatgtg cccggcaccc tgctgaatat cgctctgctg aatctgggca gcagcgaccc
     5761 ttctctgcgg agcgccgcct acaatctgct gtgcgccctg acctgcacct tcaacctgaa
     5821 gatcgagggc cagctgctgg aaaccagcgg cctgtgcatc cccgccaaca acaccctgtt
     5881 tatcgtgagc atcagcaaga ccctggccgc caacgagcct cacctgaccc tggaatttct
     5941 ggaagagtgc atcagcggct tcagcaagag cagcatcgag ctgaagcacc tgtgcctgga
     6001 atacatgacc ccctggctgt ctaatctggt ccggttctgc aagcacaacg acgacgccaa
     6061 gcggcagaga gtgaccgcca ttctggacaa gctgatcaca atgaccatca acgagaagca
     6121 gatgtacccc agcatccagg ccaagatctg gggcagcctg ggccagatca ccgatctgct
     6181 ggatgtggtg ctggacagct tcatcaagac cagcgccacc ggcggactgg gaagcatcaa
     6241 ggccgaggtg atggccgata cagccgtggc cctggctagc ggcaacgtga agctggtgtc
     6301 cagcaaggtg atcggccgga tgtgcaagat catcgacaag acctgcctga gccccacccc
     6361 taccctggaa cagcacctga tgtgggacga tatcgccatc ctggcccggt acatgctgat
     6421 gctgtccttc aacaatagcc tggacgtggc cgcccatctg ccctacctgt tccacgtggt
     6481 gacctttctg gtggccaccg gccctctgag cctgagagcc agcacccacg gcctggtgat
     6541 taacatcatc cactccctgt gcacctgctc ccagctgcac ttcagcgagg aaaccaagca
     6601 ggtgctgaga ctgagcctga ccgagttctc cctgcccaag ttctacctgc tgtttggcat
     6661 cagcaaagtg aagtctgccg ccgtgatcgc cttcaggtcc agctacaggg acagatcttt
     6721 ctcccccgga agctacgaga gagagacatt cgccctgaca agcctggaaa ccgtgacaga
     6781 ggccctgctg gaaatcatgg aagcctgcat gagagacatc cccacctgca agtggctcga
     6841 ccagtggacc gaactggccc agaggttcgc cttccagtac aaccccagcc tgcagcctag
     6901 agccctggtg gtgttcggct gcatctccaa gagagtgagc cacggccaga ttaagcagat
     6961 catcaggatc ctgtccaagg ccctggaaag ctgtctgaag ggccccgaca cctacaactc
     7021 ccaggtgctg atcgaggcca cagtgatcgc cctgaccaaa ctgcagccac tgctgaataa
     7081 ggacagcccc ctgcacaaag ccctgttctg ggtggccgtg gccgtgctgc agctggacga
     7141 ggtgaacctg tacagcgccg ggaccgctct gctggaacag aatctgcaca ccctggactc
     7201 cctgagaatc ttcaacgaca agagccccga ggaagtgttc atggccatcc ggaaccccct
     7261 ggaatggcac tgcaagcaga tggaccactt cgtgggcctg aacttcaaca gcaacttcaa
     7321 cttcgccctg gtcggacacc tgctgaaggg ctacagacac cccagcccag ccatcgtggc
     7381 caggaccgtg cggatcctgc ataccctgct gaccctggtg aacaagcacc ggaactgcga
     7441 caagtttgag gtgaacaccc agagcgtggc ctacctggct gccctgctga cagtgagcga
     7501 ggaagtgcgg agccggtgca gcctgaagca caggaagagc ctgctcctca ctgatatcag
     7561 catggaaaac gtgcccatgg acacctaccc catccaccac ggcgacccca gctacaggac
     7621 cctgaaagaa acccagccct ggtcctcccc taagggcagc gagggctatc tggccgccac
     7681 ctatcctacc gtcggccaga caagccctag ggcccgcaag tccatgagcc tggacatggg
     7741 ccagcctagc caggccaaca ccaagaaact gctgggcacc cggaagagct tcgaccacct
     7801 gatcagcgac accaaggccc ccaagaggca ggaaatggaa agcggcatca ccaccccccc
     7861 caagatgcgg agagtggccg agacagacta cgagatggaa acccagcgga tcagcagctc
     7921 ccagcagcac cctcacctgc ggaaagtgtc cgtgtccgag agcaatgtgc tgctggacga
     7981 agaggtgctg accgatccta agatccaggc cctgctgctg actgtcctgg ccaccctggt
     8041 gaagtacacc accgacgagt tcgatcagcg gatcctgtac gagtacctgg ccgaggccag
     8101 cgtggtgttt cccaaggtgt tccctgtggt gcacaatctg ctggatagca agatcaacac
     8161 cctgctgtct ctgtgccagg accccaacct gctgaatccc atccacggca tcgtgcagag
     8221 tgtggtgtac cacgaagaga gcccccctca gtaccagacc agctacctgc agtccttcgg
     8281 cttcaacggc ctgtggcggt ttgccggccc tttcagcaag cagacccaga tccccgacta
     8341 cgccgaactg atcgtgaagt tcctggacgc cctgatcgat acctacctgc ccggcatcga
     8401 cgaggaaacc tccgaggaat ctctgctgac ccccacctcc ccttatcctc cagccctgca
     8461 gtcccagctg tccatcaccg ccaacctgaa cctgtccaac tccatgacca gcctggccac
     8521 atctcagcac agccctggca tcgacaaaga aaacgtggag ctgtctccca ccaccggcca
     8581 ctgcaactcc ggcaggacca gacacggaag cgccagccag gtgcagaagc agagaagcgc
     8641 cggcagcttc aagcggaaca gcatcaagaa aatcgtgtaa tcaactttct tgtacaaagt
     8701 ggtgataatt aattaagatc gagctagcta gaagcttgtc gagaagtact agaggatcat
     8761 aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc
     8821 cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta
     8881 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     8941 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatctgatc
     9001 actgcttgag cctaggagat ccgaaccaga taagtgaaat ctagttccaa actattttgt
     9061 catttttaat tttcgtatta gcttacgacg ctacacccag ttcccatcta ttttgtcact
     9121 cttccctaaa taatccttaa aaactccatt tccacccctc ccagttccca actattttgt
     9181 ccgcccacag cggggcattt ttcttcctgt tatgttttta atcaaacatc ctgccaactc
     9241 catgtgacaa accgtcatct tcggctactt tttctctgtc acagaatgaa aatttttctg
     9301 tcatctcttc gttattaatg tttgtaattg actgaatatc aacgcttatt tgcagcctga
     9361 atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
     9421 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt
     9481 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
     9541 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt
     9601 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt
     9661 tcttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
     9721 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
     9781 acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttagg tggcactttt
     9841 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     9901 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
     9961 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
    10021 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
    10081 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
    10141 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
    10201 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
    10261 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
    10321 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
    10381 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
    10441 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
    10501 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
    10561 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
    10621 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
    10681 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
    10741 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
    10801 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
    10861 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
    10921 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
    10981 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
    11041 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
    11101 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
    11161 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
    11221 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
    11281 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
    11341 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
    11401 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
    11461 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
    11521 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
    11581 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
    11641 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
    11701 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
    11761 cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catagaccag
    11821 ccgcgtaacc tggcaaaatc ggttacggtt gagtaataaa tggatgccct gcgtaagcgg
    11881 gtgtgggcgg acaataaagt cttaaactga acaaaataga tctaaactat gacaataaag
    11941 tcttaaacta gacagaatag ttgtaaactg aaatcagtcc agttatgctg tgaaaaagca
    12001 tactggactt ttgttatggc taaagcaaac tcttcatttt ctgaagtgca aattgcccgt
    12061 cgtattaaag aggggcgtgg ccaagggcat ggtaaagact atattcgcgg cgttgtgaca
    12121 atttaccgaa caactccgcg gccgggaagc cgatctcggc ttgaacgaat tgttaggtgg
    12181 cggtacttgg gtcgatatca aagtgcatca cttcttcccg tatgcccaac tttgtataga
    12241 gagccactgc gggatcgtca ccgtaatctg cttgcacgta gatcacataa gcaccaagcg
    12301 cgttggcctc atgcttgagg agattgatga gcgcggtggc aatgccctgc ctccggtgct
    12361 cgccggagac tgcgagatca tagatataga tctcactacg cggctgctca aacttgggca
    12421 gaacgtaagc cgcgagagcg ccaacaaccg cttcttggtc gaaggcagca agcgcgatga
    12481 atgtcttact acggagcaag ttcccgaggt aatcggagtc cggctgatgt tgggagtagg
    12541 tggctacgtc tccgaactca cgaccgaaaa gatcaagagc agcccgcatg gatttgactt
    12601 ggtcagggcc gagcctacat gtgcgaatga tgcccatact tgagccacct aactttgttt
    12661 tagggcgact gccctgctgc gtaacatcgt tgctgctgcg taacatcgtt gctgctccat
    12721 aacatcaaac atcgacccac ggcgtaacgc gcttgctgct tggatgcccg aggcatagac
    12781 tgtacaaaaa aacagtcata acaagccatg aaaaccgcca ctgcgccgtt accaccgctg
    12841 cgttcggtca aggttctgga ccagttgcgt gagcgcatac gctacttgca ttacagttta
    12901 cgaaccgaac aggcttatgt caactgggtt cgtgccttca tccgtttcca cggtgtgcgt
    12961 cacccggcaa ccttgggcag cagcgaagtc gaggcatttc tgtcctggct ggcgaacgag
    13021 cgcaaggttt cggtctccac gcatcgtcag gcattggcgg ccttgctgtt cttctacggc
    13081 aaggtgctgt gcacggatct gcccttgctt caggagatcg gtagacctcg gccgtcgcgg
    13141 cgcttgccgg tggtgctgac cccggatgaa gtggttcgca tcctcggttt tctggaaggc
    13201 gagcatcgtt tgttcgccca ggactctagc tatagttcta gtggttggct actcgagatc
    13261 ccgggtgatc aagtcttcgt cgagtgattg taaataaaat gtaatttaca gtatagtatt
    13321 ttaattaata tacaaatgat ttgataataa ttcttattta actataatat attgtgttgg
    13381 gttgaattaa aggtccgtat actccggaat attaatag
//
