LOCUS       sequence-313267-       14788 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Non-infectious HIV-1 Clone with stop codon in Env (see NIH AIDS 
            Reagent Program Cat #11051) and E45A mutation in capsid (CA E45A)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14788)
  AUTHORS   Christensen DE, Ganser-Pornillos BK, Johnson JS, Pornillos O, 
            Sundquist WI
  TITLE     Reconstitution and visualization of HIV-1 capsid-dependent 
            replication and integration in vitro.
  JOURNAL   Science. 2020 Oct 9;370(6513). pii: 370/6513/eabc8420. doi: 
            10.1126/science.abc8420.
  PUBMED    33033190
REFERENCE   2  (bases 1 to 14788)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2020-10-9"; volume: "370(6513). pii: 370/6513/eabc8420. doi";
            pages: " 10.1126/science.abc8420"
COMMENT     Sequence Label: SG3-Delta-ENV CA E45A
FEATURES             Location/Qualifiers
     source          1..14788
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             212..468
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    515..640
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     CDS             1919..4930
                     /codon_start=1
                     /gene="pol"
                     /product="pol protein from human immunodeficiency virus 1"
                     /label=HIV-1 pol
                     /translation="FFREDLAFPQGKAREFSSEQTRANSSTRRELQVWGGDNNSPSEAG
                     AGRQGNVSLSFPQITLWQRPLVTIKIGGQLKEALLDTGADDTVLEEMNLPGRWKPKMIG
                     GIGGFIKVRQYDQIPIEICGHKAIGTVLVGPTPVNIIGRNLLTQIGCTLNFPISPIETV
                     PVKLKPGMDGPKVKQWPLTEEKIKALVEICTEMEKEGKISKIGPENPYNTPVFAIKKKD
                     STKWRKLVDFRELNKRTQDFWEVQLGIPHPAGLKKKKSVTVLDVGDAYFSVPLDKDFRK
                     YTAFTIPSINNETPGIRYQYNVLPQGWKGSPAIFQSSMTKILEPFRKQNPDIVIYQYMD
                     DLYVGSDLEIGQHRTKIEELRQHLLRWGFTTPDKKHQKEPPFLWMGYELHPDKWTVQPI
                     VLPEKDSWTVNDIQKLVGKLNWASQIYSGIKVKQLCKLLRGTKALTEVVTLTEEAELEL
                     AENREILKEPVHGVYYDPSKDLIAEIQKQGQGQWTYQIYQEPFKNLKTGKYARTRGAHT
                     NDVKQLTEAVQKITTECIIIWGKTPKFRLPIQKETWETWWTEYWQATWIPEWEFVNTPP
                     LVKLWYQLEKEPIEGAETFYVDGAANRETKLGKAGYVTNKGRQKVVTLTDTTNQKTELE
                     AIHLALQDSGLEVNIVTDSQYALGIIQAQPDKSESELVSQIIEQLIKKEKVYLAWVPAH
                     KGIGGNEQVDKLVSAGVRKVLFLDGIDKAQEEHEKYHSNWRAMASDFNLPPVVAKEIVA
                     SCDKCQLKGEAMHGQVDCSPGIWQLDCTHLEGKIILVAVHVASGYIEAEVIPAETGQET
                     AYFLLKLAGRWPVKTIHTDNGSNFTSTTVKAACWWAGIKQEFGIPYNPQSQGVVESMNK
                     ELKKIIGQVRDQAEHLKTAVQMAVFIHNFKRKGGIGGYSAGERIVDIIATDIQTKELQK
                     QITKIQNFRVYYRDNKDPLWKGPAKLLWKGEGAVVIQDNSDIKVVPRRKAKIIRDYGKQ
                     MAGDDCVASRQDED"
     misc_feature    4615..4732
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     RBS             6522..6533
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     misc_feature    7615..7848
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8033..8077
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     LTR             9309..9565
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     RBS             9766..9774
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     primer_bind     complement(10054..10073)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(10055..10073)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(10092..10108)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10092..10108)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10105..10127)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10116..10132
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10140..10146,10147..10164,10165..10170))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10185..10206
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10323..10340)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10494..11082)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10574..10593)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11253..12044,12045..12113))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11876..11895
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12114..12218)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      12245..12700
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(12332..12351)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     12542..12563
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     12826..12848
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     12840..12857
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     12841..12857
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gatagaagag gccaatgaag gagaaaacaa cagcttgcta caccctatga gccagcatgg
       61 gatggatgac ccggagagag aagtgctaga gtggaggttt gacagccgcc tagcatttca
      121 tcacatggcc cgagagctgc atccggagta ctacaagaac cgatgacatc gcgctttcta
      181 caagggactt tccgctgggg actttccagg ggaggcgtgg cctgggcggg accggggagt
      241 ggcgagccct cagatgctgc atataagcag ctgcttttgc ctgtactggg tctctctggt
      301 tagaccagat ctgagcctgg gagctctctg gctagctagg gaacccactg cttaagcctc
      361 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
      421 gctagagatc cctcataccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
      481 cagggaccgg aaagcgaaag agaaaccaga ggagctctct cgacgcagga ctcggcttgc
      541 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac
      601 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattgagc gggggaaaat
      661 tggataagtg ggagaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac
      721 atctagtatg ggcaagcagg gagctagaac gattcgcagt taatcccggc ctgttagaaa
      781 cagcagaagg ctgtagacaa atactgggac agctacaacc gtcccttcag acaggatcag
      841 aagaacttaa atcattatat aatacaatag cagtcctcta ttgtgtgcat caaatgatag
      901 atgtaaaaga caccaaggaa gctttagaga agatagagga agagcaaaac aacagtaaga
      961 aaaaagcaca gcaagcagca gctgacacag gaaacagcag ccaggtcagc cgaaattacc
     1021 ctatagtgca gaacatccag gggcaaatgg tacatcaggc catatcaccc agaactttaa
     1081 atgcatgggt aaaagtagta gaagagaagg ctttcagccc agaagtaata cccatgtttt
     1141 cagcattatc agcaggagcc accccacaag atttaaacac catgctaaac acagtggggg
     1201 gacatcaagc agctatgcaa atgttaaaag agaccatcaa tgaggaagct gcagaatggg
     1261 atagattgca tccagtgcaa gcagggcctg ttgcaccagg ccagatgaga gaaccaaggg
     1321 gaagtgacat agcaggaact actagtaccc ttcaggaaca aataggatgg atgacaaata
     1381 atccacctat cccagtagga gaaatctata aaagatggat aatcctggga ttaaataaaa
     1441 tagtaaggat gtatagtcct tctagcattc tggacataag acaaggacca aaggaatcct
     1501 ttagagacta tgtagacagg ttctataaaa ctttaagagc cgagcaagct tcacaggagg
     1561 taaaaaattg gatgacagaa accttgttgg tccaaaatgc gaacccagat tgtaagacca
     1621 ttttaaaagc attgggacca ggagctacac tagaggaaat gatgacagca tgtcagggag
     1681 tgggaggacc cggccacaaa gcaagagttt tggcggaagc aatgagccaa gtaacaaatt
     1741 cagccaccat aatgatgcag agaggcaatt ttaggaacca aagaaagact gttaagtgtt
     1801 tcaattgtgg caaagaaggg catatagcca gaaattgcaa ggcccctagg aaaaggggct
     1861 gctggaaatg tggaaaggaa ggacaccaaa tgaaagattg tacagagaga caggctaatt
     1921 ttttagggaa gatctggcct tcccacaagg gaaggccagg gaattttcct cagagcagac
     1981 tagagccaac agctccacca gaagagagct tcaggtttgg ggaggagaca acaactcccc
     2041 ctcagaagca ggagcgggaa gacaaggaaa tgtatccctt agcttccctc agatcactct
     2101 ttggcaacga cccctcgtca caataaagat aggggggcaa ctaaaggagg ctctattaga
     2161 tacaggagca gatgatacag tattagaaga aatgaatttg ccagggagat ggaagccaaa
     2221 aatgataggg ggaattggag gttttatcaa agtaagacag tatgatcaga tacccataga
     2281 aatctgtgga cataaagcta taggtacagt attagtagga cctacacctg tcaacataat
     2341 tggaagaaat ctgttgactc agattggttg cactctaaat tttcccatta gtcctattga
     2401 aactgtacca gtaaaattaa agccaggaat ggatggccca aaagttaaac aatggccatt
     2461 gacagaagaa aaaataaaag cattagtaga aatttgtaca gaaatggaaa aggaagggaa
     2521 gatttcaaaa attgggcctg aaaatccata caatactcca gtatttgcta taaagaaaaa
     2581 agacagtact aaatggagaa aattagtaga tttcagagaa cttaataaga ggactcaaga
     2641 cttctgggaa gttcaattag gaataccaca tcccgctgga ttaaaaaaga aaaaatcagt
     2701 aacagtacta gatgtgggtg atgcatattt ctcagttccc ttagataaag acttcaggaa
     2761 atatactgca tttaccatac ctagtataaa caatgagaca ccagggatta gatatcagta
     2821 caatgtgctc ccacagggat ggaaaggatc accagcaata ttccaaagta gcatgacaaa
     2881 aatcttagag ccttttagaa agcaaaatcc agacatagtt atctatcagt acatggatga
     2941 tttgtatgta ggatctgact tagaaatagg gcagcataga acaaaaatag aggaactgag
     3001 acaacatctg ttaaggtggg gatttaccac accagacaaa aaacatcaga aagaacctcc
     3061 attcctttgg atgggttatg aactccatcc tgataaatgg acagtacagc ctatagtgct
     3121 gccagaaaaa gacagctgga ctgtcaatga catacagaag ttagtgggaa aattgaattg
     3181 ggcaagtcag atttactcag ggatcaaagt gaagcagtta tgtaaactcc ttaggggaac
     3241 caaagcacta acagaagtag taacactaac agaagaagca gagctagaac tggcagaaaa
     3301 cagggaaatt ctaaaagaac cagtacatgg agtgtattat gacccatcaa aagacttaat
     3361 agcagaaata cagaaacagg ggcaaggcca atggacatat caaatttatc aagagccatt
     3421 taaaaatctg aaaacaggaa aatatgcaag aacgaggggt gcccacacta atgatgtaaa
     3481 acaattaaca gaggcagtgc aaaaaataac cacagaatgc ataataatat ggggaaaaac
     3541 tcctaaattt agactgccca tacaaaaaga aacatgggaa acatggtgga cagagtattg
     3601 gcaagccacc tggattcctg aatgggagtt tgtcaatacc cctcccttag tgaaattatg
     3661 gtaccagtta gagaaagagc ccatagaagg cgcagaaact ttctatgtag atggagcagc
     3721 taacagggag actaaattag gaaaagcagg atatgttact aacaaaggaa gacaaaaagt
     3781 tgtcacccta actgacacaa caaatcagaa gactgagtta gaagcaattc atctagcttt
     3841 gcaggattct ggattagaag taaacatagt aacagactca caatatgcat taggaatcat
     3901 tcaagcacaa ccagataaaa gtgaatcaga attagtcagt caaataatag agcagttaat
     3961 aaaaaaggaa aaggtctacc tggcatgggt accagcacac aaaggaattg gaggaaatga
     4021 acaagtagat aaattagtca gtgctggggt caggaaagta ctatttttag atggaataga
     4081 taaggcccaa gaagaacatg agaaatatca cagtaattgg agagccatgg ctagtgattt
     4141 taacttacca cctgtagtag caaaagaaat agtagccagc tgtgataaat gtcagctaaa
     4201 aggagaagcc atgcatggac aagtagactg tagtccagga atatggcaac tagattgcac
     4261 acatctagaa ggaaaaatta tcctggtggc ggttcatgta gccagtggat atatagaagc
     4321 agaagttatt ccagcagaga cagggcagga aacagcatac tttctcttaa aattagcagg
     4381 aagatggcca gtaaaaacaa tacatacaga caatggcagc aatttcacca gtaccacggt
     4441 taaggccgcc tgttggtggg cagggatcaa gcaggaattt ggcattccct acaatcccca
     4501 aagtcaagga gtagtagaat ctatgaataa agaattaaag aaaattatag gacaggtaag
     4561 agatcaggct gaacatctta aaacagcagt acaaatggca gtatttatcc acaattttaa
     4621 aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac
     4681 agacatacaa actaaagaac tacaaaaaca aattacaaaa attcaaaatt ttcgggttta
     4741 ttacagggac aacaaagatc cactttggaa aggaccagca aagcttctct ggaaaggtga
     4801 aggggcagta gtaatacaag ataatagtga cataaaagta gtgccaagaa gaaaagcaaa
     4861 gatcattaga gattatggaa aacagatggc aggtgatgat tgtgtggcaa gtagacagga
     4921 tgaggattag aacatggata agtttagtaa aacaccatat gtatatttca aggaaagcaa
     4981 agggatggtt ttatagacat cactatgaaa gcactcaccc aaaaataagt tcagaagtac
     5041 acatcccact aggggatgct agattggtaa taacaacata ttggggtctg catacaggag
     5101 aaagagattg gcatttgggt catggagtct ccgtagaatg gaggaaaaag agatatagca
     5161 cacaagtaga ccctgaccta gcagaccaac taattcatct gtattacttt gattgttttt
     5221 cagaatctgc cataagaaat gccatattag gacatatagt tagtcctagg tgtgaatatc
     5281 aagcaggaca taacaaggta ggatctctac agtacctagc actagcagca ttaataacac
     5341 caaaaaggat aaagccacct ttgcctagtg ttacaaaact aacagaggat agatggaaca
     5401 agccccagaa gaccaagggc cacagaggga gccatacaat gaatggacac tagagctttt
     5461 agaagaactt aagaatgaag ctgttagaca ttttcctagg atatggctcc atggcttagg
     5521 gcaatatatc tatgaaactt atggggatac ttgggcagga gtggaagccc tagtaagaac
     5581 tctgcaacaa ctgctgttta ctcttttcag aattgggtgt cgacatagca gaataggcat
     5641 tactcaacga agaagagcaa gaaatggagc cagtagatcc tagactagag ccctggaagc
     5701 atccaggaag ccagcctaaa actgcttgta ccaaatgcta ttgtaaaaag tgttgcttac
     5761 attgccaagt ttgtttcatg acaaaaggct taggcatctc ctatggcagg aagaagcgga
     5821 gacagcgacg aagagctcct caagacagtc aggctcatca agcttctcta tcaaagcagt
     5881 aagtagtgca tgtaatgcaa cctatacaaa tagcagcaat agtagcatta gtagtggtag
     5941 gaataatagc aatagttgtg tggtccatag tattaataga atataggaaa atattaagac
     6001 aaagaaaaat agacaggtta attaaaagaa taagtaaaag agcagaagac agtggcaatg
     6061 agagtgaagg agatcaggaa gaattatcag cacttgtgga gatggggcac catgctcttt
     6121 gggatattga tgatctatag tgctgcaggg aatttgtggg tcacagtata ttatggggta
     6181 cctgtatgga aagaagcaac caccactcta ttttgtgcat cagatgctaa agcatatgat
     6241 acagaggtac ataatgtttg ggccacacat gcctgtatac ccacagaccc caacccacaa
     6301 gagatagtat tagaaaatgt gacagaagat tttaacatgt ggaaaaataa catggtagaa
     6361 cagatgcagg aggatataat cagtttatgg gatcaaagcc taaagccatg tgtaaaatta
     6421 accccactct gtgttactct aaattgcact gatgagttga agaatgctac taataccact
     6481 agctagtact aataccccta gtggtagctg gaaaaagatg gaaagaggag aaataaagaa
     6541 ctgctctttc aatgtcttag gagataagaa gcagaaagca tatgcactct tttataaact
     6601 tgatgtagta ccaatagata atgataagaa tagtaccaaa tataggttga taagttgtaa
     6661 cacctcagtc actacacagg cctgtccaaa gatatccttt gagccaattc ccatacatta
     6721 ttgtgccccg gctggttttg cgattctaaa gtgtaaagat aagaagttca atggaacagg
     6781 atcatgtaaa aatgtcagca cagtacaatg tacacatgga attaagccag tagtatcaac
     6841 tcaactgctg ttaaatggca gcctagcaga agaagaggta gtaattagat ctgccaattt
     6901 ctcggacaat gctaaaacca taatagtaca gctgaaagaa gctgtagaaa ttaattgcac
     6961 aagacccaac aaaaaaacaa gaaaacgtat aactacggga ccagggagag tatattatac
     7021 aacaggagaa atagtaggag atataagaca agcacattgt aaccttagta gagcaaaatg
     7081 gaatgacact ttaagacaga tagttataaa attaagagaa caatttgaga ataaaacaat
     7141 agtctttaat caatcctcag gaggggaccc agaaattgta atgcatagtt ttaattgtgg
     7201 aggggaattt ttctactgta agtcaacaca actgtttaat agtacttggg ctgggaataa
     7261 tacttggaat agtagtgctg aaaggtcaga tgacactgga ggaaatatca cactcccatg
     7321 cagaataaaa caaattataa acatgtggca ggaagtagga aaagcaatgt atgcccctcc
     7381 catcagcgga caaattagat gtacatcaaa tattacaggg ctgctattaa caagagatgg
     7441 tggtacgagc gataccaata ccaccgagac cttcagacct ggaggaggag atatgaggga
     7501 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     7561 acccaccacg gcaaagagaa gagtggtgca aagagaaaaa agagcagtgg gaataggagc
     7621 tctgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgtt
     7681 gacggtacag gccagacaat tattgtctgg tatagtgcaa cagcagaaca atttgctgag
     7741 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     7801 ggcaagagtc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     7861 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     7921 atctctgaat cagatttggg ataacatgac ttggatgcag tgggaaagag aaattgaaaa
     7981 ttacacagac ttaatataca acttaattga agaatcgcag aaccagcaag aaaagaatga
     8041 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta caataacaaa
     8101 ctggctgtgg tatataaaaa tattcataat gatagtagga ggcttgatag gtttaagaat
     8161 agtttttact gtactttcta tagtgaatag agttaggcag ggatactcac cattgtcgtt
     8221 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatcg aagaagaagg
     8281 tggagagaga gacagagaca gatccggtcg attagtgaac ggattcttag cacttttctg
     8341 ggacgatctg cggagcctgt gcctcttcag ctaccaccgc ttgagagact taatcttggt
     8401 tgtaacgagg attgtggaac ttctgggacg cagggggtgg gaagccctca agtattggtg
     8461 gagtctccta cagtattgga gccaggaact aaagaatagt gctgttaact tgcttaatgt
     8521 cacagccata gcagtagctg agggaacaga tagggttata gaagtagtac aaagaactta
     8581 tagagctatt ctccacatac ctagaagaat aagacagggc ttggaaaggc ttttgctata
     8641 agatgggtgg caagtggtca aaacgtatgg agggtggatg gcatgctgta agggaaagaa
     8701 tgagacgagc tgagccagca gcagatgggg tgggagcagt atctagagac ctggaaaaac
     8761 atggagcaat cacaagtagc aatacagcag ctaacaatgc tgattgtgcc tggctggaag
     8821 cacaagagga ggaggaggtg ggttttccag tcagacctca ggtaccttta agaccaataa
     8881 cttacaaggc agctgtagat attagccact ttttaaaaga aaagggggga ctggaagggc
     8941 taattttctc ccaaagaaga caagatatcc ttgatctgtg gacctaccac acacaaggct
     9001 acttccctga ttggcagaac tacacaccag ggccagggat cagatatcca ctgacctttg
     9061 gatggtgttt caagctagta ccagttgatc cagagaagat agaagaggcc aatgaaggag
     9121 aaaacaacag cttgctacac cctatgagcc agcatgggat ggatgacccg gagagagaag
     9181 tgctagagtg gaggtttgac agccgcctag catttcatca catggcccga gagctgcatc
     9241 cggagtacta caagaaccga tgacatcgcg ctttctacaa gggactttcc gctggggact
     9301 ttccagggga ggcgtggcct gggcgggacc ggggagtggc gagccctcag atgctgcata
     9361 taagcagctg cttttgcctg tactgggtct ctctggttag accagatctg agcctgggag
     9421 ctctctggct agctagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     9481 caagtagtgt gtgcccgtct gttgtgtgac tctggtagct agagatccct catacccttt
     9541 tagtcagtgt ggaaaatctc tagcaaaaat tcctagctgc ctagaaataa aacactatgc
     9601 ttttgaataa catatgactt aaaagaagaa tttatgatgg ctattagaaa atattttaaa
     9661 ctgattgaaa atgaaggtga actacaatga tctagattgc tgctgctgaa atgctaagat
     9721 gaaatttata gccttaagtc tatgcattgg aaaagaaagc ttttttaagg aggtagaaaa
     9781 ggaataggaa atcaaaccta aagacagtag aagtaaagaa ataatgaaga caagagcaga
     9841 aattagtgaa tagaaagtaa tcttacatag agaaaattga caaagctaag aaaaagttgg
     9901 ttctttgaaa agattaatgt aattgaccac ttagcaaaac tgataaaagg aggatgggga
     9961 gggggagaga gaacataaat ttaaataata ggaattgaaa aatgtcacat cactacggat
    10021 cctctagagt cgacctgcag gcatgcaagc tttccctata gtgagtcgta ttagagcttg
    10081 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
    10141 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
    10201 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
    10261 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
    10321 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
    10381 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
    10441 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
    10501 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
    10561 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
    10621 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
    10681 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
    10741 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
    10801 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
    10861 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
    10921 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
    10981 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
    11041 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
    11101 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
    11161 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
    11221 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
    11281 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
    11341 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
    11401 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
    11461 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
    11521 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
    11581 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
    11641 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
    11701 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
    11761 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
    11821 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
    11881 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
    11941 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
    12001 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
    12061 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
    12121 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
    12181 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
    12241 cctgacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
    12301 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
    12361 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
    12421 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
    12481 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
    12541 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
    12601 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
    12661 tttaacgcga attttaacaa aatattaacg cttacaattt ccattcgcca ttcaggctgc
    12721 gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag
    12781 ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt
    12841 gtaaaacgac ggccagtgaa ttccttctat ggaatacttt ttaaatatat agtctgataa
    12901 tatataactg acacgttttc tgttgtttca taatggttga ttttctcagg gctacgtttc
    12961 tctatttttt cctgttctca aatcttaact ctccatctct gtcctcaagt ggtaagtgat
    13021 cttgtgtctt attttgaaat taaagtcatc aacaaattat ttcaactcat agatttatag
    13081 ttgtggaaga cttactattc aaatggaatt agaacgtttt gggggatagg atgcagtagt
    13141 tcttggtagt ccaagactct taataaaaca actagaaaag ccaggtaatt tacaaaattt
    13201 atatatttag cgatagagaa caatgggtgt aaaaatgatt aaagaaagca aaattttgca
    13261 ccctggggga atacctttca caagtgagct gagaggtctg cagctgatca ttgtttttcc
    13321 tgggtacaga gtagaggagg cctttgggct gcagcctgag tggcagattg gaaatatgag
    13381 gggcctttaa acacagagaa cgccctcagt atattcctaa attctgtagt tcagctgggc
    13441 ggaagagata aaccaaaatc tcagaaaagg agattgggat attttctagt cctatggtgt
    13501 tgagataaag attctctagg ggaagcgact tgagaacaat aggtgagagt tgaaagcgtt
    13561 tgaggaagtg gtgaatacac actgatgatt ttcaacacac ccagtttctt cccccgtaga
    13621 catttgccaa attctgaagc tgcttaggct agagagctaa accagaagtc tcttaaaaac
    13681 aaagcaattc ttatagtatc taggtaccta gaagataacg atctgctagc ttttgaaatg
    13741 aaggctctgc agagtctcat cctaggaaca gggacaaacc agaggtagtc tccttcatag
    13801 caaaactgct actcagcagt gttctatgat tctggcatta tagaggaagt agtaaaagta
    13861 tatgtatgaa aattgtgtta agtgaaggat gaataagaaa atatctaggg taaccactaa
    13921 aagaaaagaa tgtgtaacaa atgaatacag aaaaaaattg gatatagaat atgtgataaa
    13981 ttcagaagaa gacaagaaag aaaatataaa acaagcaggc aactaaatta gtaattatgt
    14041 tacatggaaa tgggctaaat atttcaagta aatggcaaaa atcatcagac tggacaaata
    14101 ataattacct aaatataagt acctagaaag attgagagaa aatgatgcga aaaggtatac
    14161 catccaaatg ctaactgaag gaaataagtg tggctatacc aatcaggcaa aggagacttt
    14221 aatacaataa acagtagtag aaaaagagaa ctacatttta tagtgtaagg ggattgatcc
    14281 accagagtta ccacaatcct ttatgtgtac ccagtaaaat agcccaaaca tctataaacc
    14341 aaaaatttta ttttgaacta aaagaaacag acaaatatgc cctcacaatg gatgcacata
    14401 tataggacat acatacatca caattagcag gcttgatcta attgtcatac gtagaacatt
    14461 gaaactatta atggtggtat taaccatctt ttcccttgca catggaaatt gtactagaat
    14521 tgaccataag ctagggccat gaacaaagcc tcagcaaata tcattgattt taataattga
    14581 atatgttgtt tgtattatag taaataaaaa taactagaaa attggaaggg ctaattatct
    14641 cccaaagaag acaagatatc cttgatctgt ggacctacca cacacaaggc tacttccctg
    14701 attggcagaa ctacacacca gggccaggga tcagatatcc actgaccttt ggatggtgtt
    14761 tcaagctagt accagttgat ccagagaa
//
