LOCUS       sequence-313269-       10672 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Baculovirus protein expression of His6-MBP-tev-Hs.NF1 ABC (2-1540).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10672)
  AUTHORS   Sherekar M, Han SW, Ghirlando R, Messing S, Drew M, Rabara D, 
            Waybright T, Juneja P, O'Neill H, Stanley CB, Bhowmik D, Ramanathan 
            A, Subramaniam S, Nissley DV, Gillette W, McCormick F, Esposito D
  TITLE     Biochemical and structural analyses reveal that the tumor suppressor
            neurofibromin (NF1) forms a high-affinity dimer.
  JOURNAL   J Biol Chem. 2020 Jan 24;295(4):1105-1119. doi: 
            10.1074/jbc.RA119.010934. Epub 2019 Dec 13.
  PUBMED    31836666
REFERENCE   2  (bases 1 to 10672)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.RA119.010934"; journalName: "J Biol Chem"; date: 
            "2020-01-24- 24"; volume: "295"; issue: "4"; pages: "1105-1119"
COMMENT     Sequence Label: R791-X12-636: His6-MBP-tev-Hs.NF1 ABC (2-1540)
FEATURES             Location/Qualifiers
     source          1..10672
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..92
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     17..34
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             146..163
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             167..1264
                     /codon_start=1
                     /gene="malE (mutated)"
                     /product="maltose binding protein from E. coli"
                     /label=MBP
                     /note="This version of the gene does not encode a signal 
                     sequence, so MBP will remain in the cytosol."
                     /translation="KIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLEE
                     KFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKLI
                     AYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIAA
                     DGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGET
                     AMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFLE
                     NYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAFW
                     YAVRTAVINAASGRQTVDEALKDAQT"
     regulatory      1121..1130
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     1238..1261
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
     protein_bind    1283..1307
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1307..1327
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     protein_bind    complement(5946..5970)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    6115..6249
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6152..6171)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6206..6225
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  6278..6443
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      6627..7081
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6714..6733)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6923..6944
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7107..7211
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7212..7280,7281..8072)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7430..7449)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8243..8831
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8732..8751
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8985..9002
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  9136..9360
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(9427..9960)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(10149..10154,10155..10171,10172..10177))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     promoter        complement(10540..10649)
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
ORIGIN
        1 atcatggaga taattaaaat gataaccatc tcgcaaataa ataagtattt tactgttttc
       61 gtaacagttt tgtaataaaa aaacctataa atattccgga ttattcatac cgtcccacca
      121 tcgggcgcgg atcaccatgg gatcccatca ccatcaccat cacggcaaaa tcgaagaagg
      181 taaactggta atctggatta acggcgataa aggctataac ggtctcgctg aagtcggtaa
      241 gaaattcgag aaagataccg gaattaaagt caccgttgag catccggata aactggaaga
      301 gaaattccca caggttgcgg caactggcga tggccctgac attatcttct gggcacacga
      361 ccgctttggt ggctacgctc aatctggcct gttggctgaa atcaccccgg acaaagcgtt
      421 ccaggacaag ctgtatccgt ttacctggga tgccgtacgt tacaacggca agctgattgc
      481 ttacccgatc gctgttgaag cgttatcgct gatttataac aaagatctgc tgccgaaccc
      541 gccaaaaacc tgggaagaga tcccggcgct ggataaagaa ctgaaagcga aaggtaagag
      601 cgcgctgatg ttcaacctgc aagaaccgta cttcacctgg ccgctgattg ctgctgacgg
      661 gggttatgcg ttcaagtatg aaaacggcaa gtacgacatt aaagacgtgg gcgtggataa
      721 cgctggcgcg aaagcgggtc tgaccttcct ggttgacctg attaaaaaca aacacatgaa
      781 tgcagacacc gattactcca tcgcagaagc tgcctttaat aaaggcgaaa cagcgatgac
      841 catcaacggc ccgtgggcat ggtccaacat cgacaccagc aaagtgaatt atggtgtaac
      901 ggtactgccg accttcaagg gtcaaccatc caaaccgttc gttggcgtgc tgagcgcagg
      961 tattaacgct gccagtccga acaaagagct ggcaaaagag ttcctcgaaa actatctgct
     1021 gactgatgaa ggtctggaag cggttaataa agacaaaccg ctgggtgccg tagcgctgaa
     1081 gtcttacgag gaagagttgg cgaaagatcc acgtattgcc gccaccatgg aaaacgccca
     1141 gaaaggtgaa atcatgccga acatcccgca gatgtccgct ttctggtatg ccgtgcgtac
     1201 tgcggtgatc aacgccgcca gcggtcgtca gactgtcgat gaagccctga aagacgcgca
     1261 gactaattcg ggatcctcga tcacaagttt gtacaaaaaa gttggcgaaa acctgtactt
     1321 ccaaggcgcc gcccacagac ctgtggaatg ggtgcaggcc gtggtgtcca gattcgacga
     1381 gcagctgccc atcaagaccg gacagcagaa tacacacacc aaggtgtcca ccgagcacaa
     1441 caaagagtgc ctgatcaaca tcagcaagta caagttcagc ctggtgatca gcggcctgac
     1501 caccatcctg aagaacgtga acaacatgcg gatcttcggc gaggccgccg agaagaacct
     1561 gtacctgagc cagctgatca tcctggacac cctggaaaag tgcctcgctg gacagcccaa
     1621 ggataccatg cggctggacg agacaatgct ggtgaagcag ctgctgcccg agatctgcca
     1681 cttcctgcac acctgccggg agggaaatca gcacgccgcc gagctgagaa atagcgccag
     1741 cggcgtgctg ttcagcctga gctgcaacaa cttcaacgcc gtgttcagcc ggatcagcac
     1801 ccggctgcag gaactgaccg tctgcagcga ggacaacgtg gacgtgcacg acatcgagct
     1861 gctgcagtac atcaacgtgg actgcgccaa gctgaagcgg ctgctgaaag agacagcctt
     1921 caagttcaag gccctgaaga aggtggccca gctggccgtg atcaacagcc tggaaaaagc
     1981 cttctggaac tgggtggaga actaccccga cgagttcacc aagctgtacc agatccccca
     2041 gaccgacatg gccgagtgcg ccgagaagct gttcgacctg gtggacggct tcgccgagag
     2101 caccaagaga aaggccgccg tgtggcccct gcagatcatc ctgctgatcc tgtgccccga
     2161 gatcatccag gacatctcca aggacgtggt ggacgagaac aacatgaaca agaagctgtt
     2221 tctggacagc ctgaggaagg ccctggccgg acacggcggc tctagacagc tgaccgagag
     2281 cgccgccatt gcctgcgtga agctgtgcaa ggccagcacc tacatcaact gggaggacaa
     2341 cagcgtgatc ttcctgctgg tgcagagcat ggtggtggac ctgaagaacc tgctgttcaa
     2401 ccccagcaag cccttcagca gaggcagcca gcccgccgac gtggacctga tgatcgactg
     2461 cctggtgtcc tgcttccgga tcagccccca caacaaccag cacttcaaga tctgcctggc
     2521 ccagaacagc cccagcacct tccactacgt gctggtgaac tccctgcacc ggatcatcac
     2581 caacagcgcc ctggactggt ggcccaagat cgacgccgtg tactgccaca gcgtggagct
     2641 gcggaacatg ttcggcgaga cactgcacaa ggccgtgcag ggatgtggag cccaccccgc
     2701 catcagaatg gcccccagcc tgaccttcaa agaaaaggtg acctccctga agttcaaaga
     2761 gaagcccacc gacctggaaa cccggtccta caagtacctg ctgctgtcca tggtgaagct
     2821 gatccacgcc gaccccaagc tgctgctgtg caaccctagg aagcagggcc ctgagacaca
     2881 gggcagcaca gccgagctga tcaccggcct ggtgcagctg gtgccccaga gccacatgcc
     2941 tgagatcgcc caggaagcca tggaagctct gctcgtcctg caccagctcg actccattga
     3001 tctgtggaac cccgacgccc ccgtggagac attctgggag atcagcagcc agatgctgtt
     3061 ctacatctgc aagaagctga ccagccatca gatgctgtcc agcaccgaga tcctgaagtg
     3121 gctgcgggag atcctgatct gccggaacaa gttcctgctg aagaacaagc aggccgaccg
     3181 gtccagctgc cactttctgc tgttttacgg cgtgggctgc gacatcccca gcagcggcaa
     3241 caccagccag atgagcatgg accacgagga actgctgaga acccctggcg ccagcctgag
     3301 aaagggcaag ggcaacagca gcatggacag cgccgctggc tgtagcggca ccccccctat
     3361 ctgtcggcag gcccagacaa aactggaagt cgctctgtac atgttcctgt ggaatcccga
     3421 taccgaggcc gtgctggtcg ccatgagctg cttccggcac ctgtgcgagg aagccgacat
     3481 cagatgcggc gtggatgagg tgtccgtgca caacctgctg cccaactaca acaccttcat
     3541 ggaattcgcc agcgtgagca acatgatgag caccggcaga gccgccctgc agaaacgggt
     3601 gatggccctg ctgcggagaa tcgagcaccc caccgccgga aatacagagg cctgggagga
     3661 tacccacgcc aagtgggagc aggccaccaa gctgatcctg aactacccca aggccaagat
     3721 ggaagatggc caggccgccg aaagcctgca caagaccatc gtgaagcggc ggatgagcca
     3781 cgtgtctggc ggcggaagca tcgacctgag cgacaccgac tccctgcaag aatggatcaa
     3841 catgaccggc tttctgtgtg ccctgggcgg agtgtgcctg cagcagcgga gcaattccgg
     3901 cctggccacc tacagccctc ctatgggccc cgtgagcgag aggaagggca gcatgatcag
     3961 cgtgatgtcc agcgagggca acgccgacac ccccgtgagc aagttcatgg accggctgct
     4021 gtccctgatg gtctgcaacc acgagaaagt gggcctgcag atccggacca acgtgaagga
     4081 cctggtcggc ctggaactga gccctgccct gtaccccatg ctgttcaata agctgaagaa
     4141 taccatctcc aagttcttcg acagccaggg ccaggtgctg ctgaccgaca ccaacaccca
     4201 gttcgtggag cagacaatcg ccatcatgaa gaatctgctg gacaaccaca ccgagggcag
     4261 ctctgaacac ctgggccagg ccagcatcga gacaatgatg ctgaacctgg tccgctacgt
     4321 gcgggtgctg ggcaacatgg tgcacgccat ccagatcaag acaaagctgt gccagctggt
     4381 cgaggtgatg atggccagac gggacgacct gagcttctgc caggaaatga agttccggaa
     4441 caagatggtg gagtacctga ccgactgggt gatgggcacc agcaaccagg ccgctgacga
     4501 cgacgtgaag tgcctgacca gggacctgga ccaggcttct atggaagctg tcgtcagtct
     4561 gctggccgga ctgcctctgc agcctgaaga aggtgacggc gtcgagctga tggaagccaa
     4621 gagccagctg ttcctgaagt acttcaccct gttcatgaac ctgctgaacg actgcagcga
     4681 ggtggaggat gagagcgccc agaccggcgg cagaaagcgg ggcatgagca gaaggctggc
     4741 ctccctgagg cactgtaccg tgctggccat gtccaatctg ctgaacgcca acgtggatag
     4801 cggcctgatg cacagcatcg gcctgggcta ccacaaggac ctgcagaccc gggccacctt
     4861 tatggaagtg ctgaccaaga tcctgcagca gggcaccgag ttcgacaccc tggccgagac
     4921 tgtgctggcc gacagattcg agagactggt ggaactggtg accatgatgg gcgaccaggg
     4981 cgagctgcct attgccatgg ccctggccaa cgtggtgccc tgtagccagt gggatgagct
     5041 ggccagggtc ctcgtcacac tgttcgacag ccggcacctg ctgtaccagc tgctgtggaa
     5101 tatgttcagc aaagaggtgg agctggccga ctccatgcag accctgttca gaggcaactc
     5161 cctggccagc aagatcatga ccttttgctt caaggtgtac ggcgccacct acctgcagaa
     5221 gctgctggac cccctgctga ggatcgtgat caccagcagc gactggcagc acgtgagctt
     5281 cgaggtggac cccaccagac tggaacccag cgagagcctg gaagagaacc agcggaacct
     5341 gctgcagatg accgagaagt tcttccacgc catcatcagc agcagctccg agttcccccc
     5401 ccagctgaga agcgtgtgcc actgcctgta ccaggtggtg tcccagcggt tcccccagaa
     5461 tagcatcggc gccgtgggca gcgccatgtt cctgcggttc atcaaccctg ccatcgtgag
     5521 cccttacgag gccggcatcc tggataagaa gccccctccc cggatcgaga ggggcctgaa
     5581 gctgatgagc aagattctgc agagcattgc caaccacgtg ctgtttacca aggaagagca
     5641 catgcggccc ttcaacgact tcgtgaagag caacttcgac gccgccaggc ggttcttcct
     5701 ggatatcgcc agcgactgcc ctaccagcga cgccgtgaac cacagcctgt ccttcatcag
     5761 cgacggcaac gtcctggccc tgcatagact gctgtggaac aaccaggaaa agatcggcca
     5821 gtacctgagc agcaaccggg accacaaggc tgtgggcagg cggcccttcg ataagatggc
     5881 caccctgctg gcctatctgg gccctcccga gcacaagcct gtggccgaca cccattggtc
     5941 tagctaatca actttcttgt acaaagtggt gatcgagcta gctagaagct tgtcgagaag
     6001 tactagagga tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac
     6061 ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg
     6121 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     6181 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
     6241 gtctggatct gatcactgct tgagcctagg agatccgaac cagataagtg aaatctagtt
     6301 ccaaactatt ttgtcatttt taattttcgt attagcttac gacgctacac ccagttccca
     6361 tctattttgt cactcttccc taaataatcc ttaaaaactc catttccacc cctcccagtt
     6421 cccaactatt ttgtccgccc acagcggggc atttttcttc ctgttatgtt tttaatcaaa
     6481 catcctgcca actccatgtg acaaaccgtc atcttcggct actttttctc tgtcacagaa
     6541 tgaaaatttt tctgtcatct cttcgttatt aatgtttgta attgactgaa tatcaacgct
     6601 tatttgcagc ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg
     6661 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
     6721 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
     6781 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
     6841 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
     6901 gttggagtcc acgttcttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
     6961 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
     7021 aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt
     7081 taggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     7141 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     7201 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     7261 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     7321 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     7381 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     7441 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     7501 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     7561 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     7621 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     7681 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     7741 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     7801 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     7861 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     7921 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     7981 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     8041 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     8101 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     8161 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     8221 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     8281 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     8341 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     8401 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     8461 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     8521 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     8581 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     8641 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     8701 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     8761 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     8821 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     8881 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     8941 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     9001 aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac
     9061 accgcataga ccagccgcgt aacctggcaa aatcggttac ggttgagtaa taaatggatg
     9121 ccctgcgtaa gcgggtgtgg gcggacaata aagtcttaaa ctgaacaaaa tagatctaaa
     9181 ctatgacaat aaagtcttaa actagacaga atagttgtaa actgaaatca gtccagttat
     9241 gctgtgaaaa agcatactgg acttttgtta tggctaaagc aaactcttca ttttctgaag
     9301 tgcaaattgc ccgtcgtatt aaagaggggc gtggccaagg gcatggtaaa gactatattc
     9361 gcggcgttgt gacaatttac cgaacaactc cgcggccggg aagccgatct cggcttgaac
     9421 gaattgttag gtggcggtac ttgggtcgat atcaaagtgc atcacttctt cccgtatgcc
     9481 caactttgta tagagagcca ctgcgggatc gtcaccgtaa tctgcttgca cgtagatcac
     9541 ataagcacca agcgcgttgg cctcatgctt gaggagattg atgagcgcgg tggcaatgcc
     9601 ctgcctccgg tgctcgccgg agactgcgag atcatagata tagatctcac tacgcggctg
     9661 ctcaaacttg ggcagaacgt aagccgcgag agcgccaaca accgcttctt ggtcgaaggc
     9721 agcaagcgcg atgaatgtct tactacggag caagttcccg aggtaatcgg agtccggctg
     9781 atgttgggag taggtggcta cgtctccgaa ctcacgaccg aaaagatcaa gagcagcccg
     9841 catggatttg acttggtcag ggccgagcct acatgtgcga atgatgccca tacttgagcc
     9901 acctaacttt gttttagggc gactgccctg ctgcgtaaca tcgttgctgc tgcgtaacat
     9961 cgttgctgct ccataacatc aaacatcgac ccacggcgta acgcgcttgc tgcttggatg
    10021 cccgaggcat agactgtaca aaaaaacagt cataacaagc catgaaaacc gccactgcgc
    10081 cgttaccacc gctgcgttcg gtcaaggttc tggaccagtt gcgtgagcgc atacgctact
    10141 tgcattacag tttacgaacc gaacaggctt atgtcaactg ggttcgtgcc ttcatccgtt
    10201 tccacggtgt gcgtcacccg gcaaccttgg gcagcagcga agtcgaggca tttctgtcct
    10261 ggctggcgaa cgagcgcaag gtttcggtct ccacgcatcg tcaggcattg gcggccttgc
    10321 tgttcttcta cggcaaggtg ctgtgcacgg atctgccctt gcttcaggag atcggtagac
    10381 ctcggccgtc gcggcgcttg ccggtggtgc tgaccccgga tgaagtggtt cgcatcctcg
    10441 gttttctgga aggcgagcat cgtttgttcg cccaggactc tagctatagt tctagtggtt
    10501 ggctactcga gatcccgggt gatcaagtct tcgtcgagtg attgtaaata aaatgtaatt
    10561 tacagtatag tattttaatt aatatacaaa tgatttgata ataattctta tttaactata
    10621 atatattgtg ttgggttgaa ttaaaggtcc gtatactccg gaatattaat ag
//
