LOCUS       sequence-313288-        1152 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of mutated membrane scaffold protein 
            (""ultra-bright"" MSP1D1) for Nanodisc formation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1152)
  AUTHORS   McLean MA, Denisov IG, Grinkova YV, Sligar SG
  TITLE     Dark, Ultra-Dark and Ultra-Bright Nanodiscs for membrane protein 
            investigations.
  JOURNAL   Anal Biochem. 2020 Oct 15;607:113860. doi: 10.1016/j.ab.2020.113860.
            Epub 2020 Aug 1.
  PUBMED    32750355
REFERENCE   2  (bases 1 to 1152)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Anal 
            Biochem."; date: "2020-10-15"; volume: "607:113860. doi"; pages: " 
            10.1016/j.ab.2020.113860. Epub 2020 Aug 1"
COMMENT     Sequence Label: pUB-MSP1D1
FEATURES             Location/Qualifiers
     source          1..1152
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             28..50
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             63..83
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=7xHis
                     /translation="HHHHHHH"
     CDS             105..125
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             715..732
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(785..803)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      799..846
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(970..989)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 attggcggaa attccctcta gaataatttt gtttaacttt aagaaggaga tataccatgg
       61 gtcatcatca tcatcatcat cacgattatg atattcctac tactgagaat ttgtattttc
      121 agggttctac cttcagtaaa cttcgcgaac aactgggccc cgtgacgcag gaattctggg
      181 acaacctgga aaaagaaacc gagggactgc gtcaggaaat gtccaaagat ttagaagagg
      241 tgaaggccaa ggttcagcca tatctcgatg actttcagaa aaaatggcag gaagagatgg
      301 aattatatcg tcaaaaggtg gaaccgctgc gtgcggaact gcaagagggg gcacgccaaa
      361 aactccatga gctctgggag aagctcagcc cattaggcga agaaatgcgc gatcgcgccc
      421 gtgcacatgt tgatgcactc cggactcatt tggcgccgta ttcggatgaa cttcgccagc
      481 gtttggccgc acgtctcgag gcgtggaaag aaaacggggg tgcccgcttg gctgagtacc
      541 acgcgaaagc gacagaacac ctgagcacct tgagcgaaaa agcgaaaccg gcgctggaag
      601 atctacgcca gggcttattg cctgttcttg agagctttaa agtcagtttt ctgtcagctc
      661 tggaagaatg gactaaaaag ctgaataccc agtaagcttg cggccgcact cgagcaccac
      721 caccaccacc actgagatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct
      781 gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt
      841 tttttgctga aaggaggaac tatatccgga ttggcgaatg ggacgcgccc tgtagcggcg
      901 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc
      961 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
     1021 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
     1081 accccaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
     1141 ttttcgccct tt
//
