LOCUS       sequence-31329-e         912 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 912)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 912)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PRDM1
FEATURES             Location/Qualifiers
     source          1..912
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             49..66
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             79..99
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             687..704
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(757..775)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      771..818
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 gaattccctc tgaatatttt gtttacttta agaaggagat ataccatgca tcatcatcat
       61 catcacagca gcggcagaga aaacttgtat ttccagggca aaatggatat ggaagacgcg
      121 gacatgaccc tgtggacgga ggccgaattt gaggaaaagt gcacttatat tgtgaatgac
      181 catccgtggg atagcggcgc agatggtggg acatctgttc aggctgaagc gagtttacca
      241 cgtaacttgc ttttcaaata cgccaccaat tcagaggaag tcatcggagt aatgtccaaa
      301 gagtatatac ctaagggcac gcgctttggt cccctcattg gggaaatcta cactaacgat
      361 acagtgccga aaaatgcaaa ccggaaatat ttctggagaa tttactcgcg tggagaactg
      421 caccatttta tcgacggctt caatgaggaa aagagcaact ggatgcgcta tgttaatccg
      481 gctcactctc cacgagagca aaacctggcg gcctgtcaga atggtatgaa catatacttt
      541 tataccatta aaccgatccc ggcaaatcag gaactgttag tctggtattg ccgtgatttc
      601 gctgagcgct tgcattaccc ttatcccggc gaacttacga tgatgaacct cacccaatga
      661 tgacgaagct tgcggccgca ctcgagcacc accaccacca ccactgagat ccggctgcta
      721 acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac
      781 cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg
      841 gattggcgaa tgggacgcgc cctgtagcgg cgcattaagc gcggcggggt gtggtggtta
      901 cgcgcagcgt ga
//
