LOCUS       sequence-313293-       10259 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian Expression of Nuclear HOXD13 (+7A) IDR-YFP with SV40 NLS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10259)
  AUTHORS   Basu S, Mackowiak SD, Niskanen H, Knezevic D, Asimi V, Grosswendt S,
            Geertsema H, Ali S, Jerkovic I, Ewers H, Mundlos S, Meissner A, 
            Ibrahim DM, Hnisz D
  TITLE     Unblending of Transcriptional Condensates in Human Repeat Expansion 
            Disease.
  JOURNAL   Cell. 2020 May 28;181(5):1062-1079.e30. doi: 
            10.1016/j.cell.2020.04.018. Epub 2020 May 7.
  PUBMED    32386547
REFERENCE   2  (bases 1 to 10259)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.04.018"; journalName: "Cell"; date: 
            "2020-05-28- 28"; volume: "181"; issue: "5"; pages: "1062-1079.e30"
COMMENT     Sequence Label: pHR-HOXD13(+7A)-IDR-YFP-NLS
FEATURES             Location/Qualifiers
     source          1..10259
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    47..172
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    669..902
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1087..1131
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1416..1533
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1686..2093
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     CDS             join(2642..2644,2645..2647,2648..3358)
                     /codon_start=1
                     /product="enhanced YFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2687..2708)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2948..2967)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3295..3316
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             3368..3388
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    3489..4077
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3542..3562)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3960..3971)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4080..4096)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4081..4097)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             4187..4420
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(4445..4463)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4446..4463)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4753..4772)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(4986..5005)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5105..5127
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5165..5183)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5251..5355
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5356..5424,5425..6216)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGTLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5574..5593)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6387..6975
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6876..6895
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7129..7146
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(7216..7236)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7221..7550
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7401..7536
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          8684..8749
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             8879..8899
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    9324..9458
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9361..9380)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9415..9434
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             9626..10259
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 gtggcgcccg aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag
       61 gactcggctt gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc
      121 aaaaattttg actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa
      181 gcgggggaga attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa
      241 atataaatta aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc
      301 tggcctgtta gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct
      361 tcagacagga tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt
      421 gcatcaaagg atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca
      481 aaacaaaagt aagaccaccg cacagcaagc ggccggccgc tgatcttcag acctggagga
      541 ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa
      601 ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca
      661 gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca
      721 gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag
      781 aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc
      841 atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc
      901 ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct
      961 agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac
     1021 agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag
     1081 caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg
     1141 tttaacataa caaattggct gtggtatata aaattattca taatgatagt aggaggcttg
     1201 gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat
     1261 tcaccattat cgtttcagac ccacctccca accccgaggg gacccgacag gcccgaagga
     1321 atagaagaag aaggtggaga gagagacaga gacagatcca ttcgattagt gaacggatct
     1381 cgacggtatc gccaaatggc agtattcatc cacaatttta aaagaaaagg ggggattggg
     1441 gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa
     1501 ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga cagcagagat
     1561 ccagtttgga tcgataagct tgatatcgaa ttcctgcagc cccgataaaa taaaagattt
     1621 tatttagtct ccagaaaaag gggggaatga aagaccccac ctgtaggttt ggcaagctag
     1681 ctgcagtaac gccattttgc aaggcatgga aaaataccaa accaagaata gagaagttca
     1741 gatcaagggc gggtacatga aaatagctaa cgttgggcca aacaggatat ctgcggtgag
     1801 cagtttcggc cccggcccgg ggccaagaac agatggtcac cgcagtttcg gccccggccc
     1861 gaggccaaga acagatggtc cccagatatg gcccaaccct cagcagtttc ttaagaccca
     1921 tcagatgttt ccaggctccc ccaaggacct gaaatgaccc tgcgccttat ttgaattaac
     1981 caatcagcct gcttctcgct tctgttcgcg cgcttctgct tcccgagctc tataaaagag
     2041 ctcacaaccc ctcactcggc gcgccagtcc tccgacagac tgagtcgccc gggggggatc
     2101 tggagctctc gagaattctc acgcgtcaag tggagcaagg caggtggaca gtggatccgg
     2161 agctaccatg tcccgagccg gtagctggga catggacggc ctgcgagcgg acggaggcgg
     2221 agctggagga gctcccgcat caagtagtag ctcaagtgtg gccgctgccg cggctagcgg
     2281 acaatgtagg gggtttttgt cagcgcctgt ctttgcgggc acacattccg ggagggccgc
     2341 tgctgcggct gctgctgctg ctgcagccgc ggcggcagct gcagctgcgg ccgctgccgc
     2401 tagtggattt gcctatcctg ggacgagtga gcgcactggt tcatcatcct catcttcatc
     2461 cagtgcggta gtcgccgctc ggccagaagc accacctgca aaagagtgtc cggccccgac
     2521 gccagctgca gcagcagcgg caccgccttc agctcctgcg ggtaccggag gcgggatggt
     2581 ttccaaagga gaggaggata atatggctat aattaaagag tttatgcggt tcaaggtgca
     2641 tatggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga
     2701 cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta
     2761 cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac
     2821 cctcgtgacc accttcggct acggcctgat gtgcttcgcc cgctaccccg accacatgaa
     2881 gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt
     2941 cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct
     3001 ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca
     3061 caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa
     3121 cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc
     3181 cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca
     3241 ctacctgagc taccagtcca agctgagcaa agaccccaac gagaagcgcg atcacatggt
     3301 cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaaggg
     3361 aggcggtcca aaaaagaaga gaaaggtatg agcggccgca actcccacct gcaacatgcg
     3421 tgactgactg aggccgcgac tctagagtcg acctgcaggc atgcaagctt gatatcaagc
     3481 ttatcgataa tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact
     3541 atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg
     3601 cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg
     3661 aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa
     3721 cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc
     3781 ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg
     3841 ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt
     3901 ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt
     3961 cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc
     4021 cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcatc
     4081 gataccgtcg acctcgaggg aattaattcg agctcggtac ctttaagacc aatgacttac
     4141 aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt
     4201 cactcccaac gaagacaaga tctgcttttt gcttgtactg ggtctctctg gttagaccag
     4261 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc
     4321 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga
     4381 tccctcagac ccttttagtc agtgtggaaa atctctagca gcatctagaa ttaattccgt
     4441 gtattctata gtgtcaccta aatcgtatgt gtatgataca taaggttatg tattaattgt
     4501 agccgcgttc taacgacaat atgtacaagc ctaattgtgt agcatctggc ttactgaagc
     4561 agaccctatc atctctctcg taaactgccg tcagagtcgg tttggttgga cgaaccttct
     4621 gagtttctgg taacgccgtc ccgcacccgg aaatggtcag cgaaccaatc agcagggtca
     4681 tcgctagcca gatcctctac gccggacgca tcgtggccgg catcaccggc gccacaggtg
     4741 cggttgctgg cgcctatatc gccgacatca ccgatgggga agatcgggct cgccacttcg
     4801 ggctcatgag cgcttgtttc ggcgtgggta tggtggcagg ccccgtggcc gggggactgt
     4861 tgggcgccat ctccttgcat gcaccattcc ttgcggcggc ggtgctcaac ggcctcaacc
     4921 tactactggg ctgcttccta atgcaggagt cgcataaggg agagcgtcga atggtgcact
     4981 ctcagtacaa tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc
     5041 gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc
     5101 gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga
     5161 aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag
     5221 acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     5281 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     5341 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
     5401 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
     5461 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
     5521 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
     5581 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
     5641 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
     5701 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
     5761 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
     5821 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
     5881 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
     5941 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
     6001 ggaacacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
     6061 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
     6121 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
     6181 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     6241 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     6301 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     6361 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     6421 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     6481 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     6541 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     6601 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     6661 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     6721 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     6781 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     6841 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     6901 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     6961 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     7021 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     7081 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
     7141 agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
     7201 cattaatgca gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag
     7261 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc
     7321 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag
     7381 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
     7441 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc
     7501 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagcttggac
     7561 acaagacagg cttgcgagat atgtttgaga ataccacttt atcccgcgtc agggagaggc
     7621 agtgcgtaaa aagacgcgga ctcatgtgaa atactggttt ttagtgcgcc agatctctat
     7681 aatctcgcgc aacctatttt cccctcgaac actttttaag ccgtagataa acaggctggg
     7741 acacttcaca tgagcgaaaa atacatcgtc acctgggaca tgttgcagat ccatgcacgt
     7801 aaactcgcaa gccgactgat gccttctgaa caatggaaag gcattattgc cgtaagccgt
     7861 ggcggtctgt accgggtgcg ttactggcgc gtgaactggg tattcgtcat gtcgataccg
     7921 tttgtatttc cagctacgat cacgacaacc agcgcgagct taaagtgctg aaacgcgcag
     7981 aaggcgatgg cgaaggcttc atcgttattg atgacctggt ggataccggt ggtactgcgg
     8041 ttgcgattcg tgaaatgtat ccaaaagcgc actttgtcac catcttcgca aaaccggctg
     8101 gtcgtccgct ggttgatgac tatgttgttg atatcccgca agatacctgg attgaacagc
     8161 cgtgggatat gggcgtcgta ttcgtcccgc caatctccgg tcgctaatct tttcaacgcc
     8221 tggcactgcc gggcgttgtt ctttttaact tcaggcgggt tacaatagtt tccagtaagt
     8281 attctggagg ctgcatccat gacacaggca aacctgagcg aaaccctgtt caaaccccgc
     8341 tttaaacatc ctgaaacctc gacgctagtc cgccgcttta atcacggcgc acaaccgcct
     8401 gtgcagtcgg cccttgatgg taaaaccatc cctcactggt atcgcatgat taaccgtctg
     8461 atgtggatct ggcgcggcat tgacccacgc gaaatcctcg acgtccaggc acgtattgtg
     8521 atgagcgatg ccgaacgtac cgacgatgat ttatacgata cggtgattgg ctaccgtggc
     8581 ggcaactgga tttatgagtg ggccccggat ctttgtgaag gaaccttact tctgtggtgt
     8641 gacataattg gacaaactac ctacagagat ttaaagctct aaggtaaata taaaattttt
     8701 aagtgtataa tgtgttaaac tactgattct aattgtttgt gtattttaga ttccaaccta
     8761 tggaactgat gaatgggagc agtggtggaa tgcctttaat gaggaaaacc tgttttgctc
     8821 agaagaaatg ccatctagtg atgatgaggc tactgctgac tctcaacatt ctactcctcc
     8881 aaaaaagaag agaaaggtag aagaccccaa ggactttcct tcagaattgc taagtttttt
     8941 gagtcatgct gtgtttagta atagaactct tgcttgcttt gctatttaca ccacaaagga
     9001 aaaagctgca ctgctataca agaaaattat ggaaaaatat tctgtaacct ttataagtag
     9061 gcataacagt tataatcata acatactgtt ttttcttact ccacacaggc atagagtgtc
     9121 tgctattaat aactatgctc aaaaattgtg tacctttagc tttttaattt gtaaaggggt
     9181 taataaggaa tatttgatgt atagtgcctt gactagagat cataatcagc cataccacat
     9241 ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata
     9301 aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa
     9361 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     9421 tgtccaaact catcaatgta tcttatcatg tctggatcaa ctggataact caagctaacc
     9481 aaaatcatcc caaacttccc accccatacc ctattaccac tgccaattac ctgtggtttc
     9541 atttactcta aacctgtgat tcctctgaat tattttcatt ttaaagaaat tgtatttgtt
     9601 aaatatgtac tacaaactta gtagttggaa gggctaattc actcccaaag aagacaagat
     9661 atccttgatc tgtggatcta ccacacacaa ggctacttcc ctgattagca gaactacaca
     9721 ccagggccag gggtcagata tccactgacc tttggatggt gctacaagct agtaccagtt
     9781 gagccagata aggtagaaga ggccaataaa ggagagaaca ccagcttgtt acaccctgtg
     9841 agcctgcatg ggatggatga cccggagaga gaagtgttag agtggaggtt tgacagccgc
     9901 ctagcatttc atcacgtggc ccgagagctg catccggagt acttcaagaa ctgctgatat
     9961 cgagcttgct acaagggact ttccgctggg gactttccag ggaggcgtgg cctgggcggg
    10021 actggggagt ggcgagccct cagatcctgc atataagcag ctgctttttg cctgtactgg
    10081 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
    10141 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
    10201 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagca
//
