LOCUS       sequence-313297-       11747 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian Expression of Nuclear Opto HOXD13(+9A) IDR with SV40 NLS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11747)
  AUTHORS   Basu S, Mackowiak SD, Niskanen H, Knezevic D, Asimi V, Grosswendt S,
            Geertsema H, Ali S, Jerkovic I, Ewers H, Mundlos S, Meissner A, 
            Ibrahim DM, Hnisz D
  TITLE     Unblending of Transcriptional Condensates in Human Repeat Expansion 
            Disease.
  JOURNAL   Cell. 2020 May 28;181(5):1062-1079.e30. doi: 
            10.1016/j.cell.2020.04.018. Epub 2020 May 7.
  PUBMED    32386547
REFERENCE   2  (bases 1 to 11747)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.04.018"; journalName: "Cell"; date: 
            "2020-05-28- 28"; volume: "181"; issue: "5"; pages: "1062-1079.e30"
COMMENT     Sequence Label: pHR-HOXD13(+9A)-IDR-mCherry-CRY2-NLS
FEATURES             Location/Qualifiers
     source          1..11747
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    497..730
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             915..959
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1244..1361
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1514..1921
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     CDS             3130..4623
                     /codon_start=1
                     /product="N-terminal photolyase homology region of 
                     Arabidopsis thaliana cryptochrome 2"
                     /label=CRY2PHR
                     /note="binds to CIB1 upon exposure to blue light (Kennedy 
                     et al., 2010)"
                     /translation="MKMDKKTIVWFRRDLRIEDNPALAAAAHEGSVFPVFIWCPEEEGQ
                     FYPGRASRWWMKQSLAHLSQSLKALGSDLTLIKTHNTISAILDCIRVTGATKVVFNHLY
                     DPVSLVRDHTVKEKLVERGISVQSYNGDLLYEPWEIYCEKGKPFTSFNSYWKKCLDMSI
                     ESVMLPPPWRLMPITAAAEAIWACSIEELGLENEAEKPSNALLTRAWSPGWSNADKLLN
                     EFIEKQLIDYAKNSKKVVGNSTSLLSPYLHFGEISVRHVFQCARMKQIIWARDKNSEGE
                     ESADLFLRGIGLREYSRYICFNFPFTHEQSLLSHLRFFPWDADVDKFKAWRQGRTGYPL
                     VDAGMRELWATGWMHNRIRVIVSSFAVKFLLLPWKWGMKYFWDTLLDADLECDILGWQY
                     ISGSIPDGHELDRLDNPALQGAKYDPEGEYIRQWLPELARLPTEWIHHPWDAPLTVLKA
                     SGVELGTNYAKPIVDIDTARELLAKAISRTREAQIMIGAA"
     CDS             4720..4740
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    4805..5393
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4858..4878)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5276..5287)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5396..5412)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5397..5413)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             5503..5736
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(5761..5779)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(5762..5779)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(6069..6088)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(6302..6321)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6421..6443
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6481..6499)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6567..6671
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6672..6740,6741..7532)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGTLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6890..6909)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7703..8291
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8192..8211
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8445..8462
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(8532..8552)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8537..8866
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8717..8852
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          10000..10065
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             10195..10215
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    10640..10774
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10677..10696)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10731..10750
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             10942..11575
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    11622..11747
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
ORIGIN
        1 agtattaagc gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga
       61 aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca
      121 gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa
      181 ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc
      241 tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag
      301 gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg ccggccgctg atcttcagac
      361 ctggaggagg agatatgagg gacaattgga gaagtgaatt atataaatat aaagtagtaa
      421 aaattgaacc attaggagta gcacccacca aggcaaagag aagagtggtg cagagagaaa
      481 aaagagcagt gggaatagga gctttgttcc ttgggttctt gggagcagca ggaagcacta
      541 tgggcgcagc gtcaatgacg ctgacggtac aggccagaca attattgtct ggtatagtgc
      601 agcagcagaa caatttgctg agggctattg aggcgcaaca gcatctgttg caactcacag
      661 tctggggcat caagcagctc caggcaagaa tcctggctgt ggaaagatac ctaaaggatc
      721 aacagctcct ggggatttgg ggttgctctg gaaaactcat ttgcaccact gctgtgcctt
      781 ggaatgctag ttggagtaat aaatctctgg aacagatttg gaatcacacg acctggatgg
      841 agtgggacag agaaattaac aattacacaa gcttaataca ctccttaatt gaagaatcgc
      901 aaaaccagca agaaaagaat gaacaagaat tattggaatt agataaatgg gcaagtttgt
      961 ggaattggtt taacataaca aattggctgt ggtatataaa attattcata atgatagtag
     1021 gaggcttggt aggtttaaga atagtttttg ctgtactttc tatagtgaat agagttaggc
     1081 agggatattc accattatcg tttcagaccc acctcccaac cccgagggga cccgacaggc
     1141 ccgaaggaat agaagaagaa ggtggagaga gagacagaga cagatccatt cgattagtga
     1201 acggatctcg acggtatcgc caaatggcag tattcatcca caattttaaa agaaaagggg
     1261 ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa
     1321 ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca
     1381 gcagagatcc agtttggatc gataagcttg atatcgaatt cctgcagccc cgataaaata
     1441 aaagatttta tttagtctcc agaaaaaggg gggaatgaaa gaccccacct gtaggtttgg
     1501 caagctagct gcagtaacgc cattttgcaa ggcatggaaa aataccaaac caagaataga
     1561 gaagttcaga tcaagggcgg gtacatgaaa atagctaacg ttgggccaaa caggatatct
     1621 gcggtgagca gtttcggccc cggcccgggg ccaagaacag atggtcaccg cagtttcggc
     1681 cccggcccga ggccaagaac agatggtccc cagatatggc ccaaccctca gcagtttctt
     1741 aagacccatc agatgtttcc aggctccccc aaggacctga aatgaccctg cgccttattt
     1801 gaattaacca atcagcctgc ttctcgcttc tgttcgcgcg cttctgcttc ccgagctcta
     1861 taaaagagct cacaacccct cactcggcgc gccagtcctc cgacagactg agtcgcccgg
     1921 gggggatctg gagctctcga gaattctcac gcgtcaagtg gagcaaggca ggtggacagt
     1981 ggatccggag ctaccatgtc ccgagccggt agctgggaca tggacggcct gcgagcggac
     2041 ggaggcggag ctggaggagc tcccgcatca agtagtagct caagtgtggc cgctgccgcg
     2101 gctagcggac aatgtagggg gtttttgtca gcgcctgtct ttgcgggcac acattccggg
     2161 agggccgctg ctgcggctgc tgctgctgct gcagccgcgg ctgcagctgc agctgcggcc
     2221 gcagcagctg ccgctagtgg atttgcctat cctgggacga gtgagcgcac tggttcatca
     2281 tcctcatctt catccagtgc ggtagtcgcc gctcggccag aagcaccacc tgcaaaagag
     2341 tgtccggccc cgacgccagc tgcagcagca gcggcaccgc cttcagctcc tgcgggtacc
     2401 ggaggcggga tggtttccaa aggagaggag gataatatgg ctataattaa agagtttatg
     2461 cggttcaagg tgcatatgga gggatctgtg aacggtcacg agttcgaaat cgagggcgaa
     2521 ggcgaggggc gaccctatga agggacacag acggccaaac tgaaggtgac caagggtggc
     2581 cccctgcctt tcgcctggga catcctgtca ccccagttta tgtacggctc taaggcttac
     2641 gttaagcacc ctgccgatat tccggactac ttgaaactga gcttccctga agggttcaag
     2701 tgggaacggg taatgaattt tgaggatggc ggtgtagtca cagttaccca ggacagctct
     2761 cttcaggacg gagaatttat ctataaggtt aaacttcggg gcactaactt cccatcagac
     2821 ggccccgtga tgcagaagaa aactatgggc tgggaagcca gctcagaacg catgtatccc
     2881 gaggacggag cactgaaggg agaaatcaag cagcgactca agctgaagga tgggggacat
     2941 tatgatgctg aggtcaaaac cacctataag gccaagaaac ccgttcagct tcctggggca
     3001 tacaacgtga atatcaaact ggatattaca tcccataacg aagactatac catcgtggaa
     3061 cagtacgagc gggccgaggg cagacatagc acagggggca tggatgaatt gtacaaggcg
     3121 gccacgcgta tgaagatgga caaaaagact atagtttggt ttagaagaga cctaaggatt
     3181 gaggataatc ctgcattagc agcagctgct cacgaaggat ctgtttttcc tgtcttcatt
     3241 tggtgtcctg aagaagaagg acagttttat cctggaagag cttcaagatg gtggatgaaa
     3301 caatcacttg ctcacttatc tcaatccttg aaggctcttg gatctgacct cactttaatc
     3361 aaaacccaca acacgatttc agcgatcttg gattgtatcc gcgttaccgg tgctacaaaa
     3421 gtcgtcttta accacctcta tgatcctgtt tcgttagttc gggaccatac cgtaaaggag
     3481 aagctggtgg aacgtgggat ctctgtgcaa agctacaatg gagatctatt gtatgaaccg
     3541 tgggagatat actgcgaaaa gggcaaacct tttacgagtt tcaattctta ctggaagaaa
     3601 tgcttagata tgtcgattga atccgttatg cttcctcctc cttggcggtt gatgccaata
     3661 actgcagcgg ctgaagcgat ttgggcgtgt tcgattgaag aactagggct ggagaatgag
     3721 gccgagaaac cgagcaatgc gttgttaact agagcttggt ctccaggatg gagcaatgct
     3781 gataagttac taaatgagtt catcgagaag cagttgatag attatgcaaa gaacagcaag
     3841 aaagttgttg ggaattctac ttcactactt tctccgtatc tccatttcgg ggaaataagc
     3901 gtcagacacg ttttccagtg tgcccggatg aaacaaatta tatgggcaag agataagaac
     3961 agtgaaggag aagaaagtgc agatcttttt cttaggggaa tcggtttaag agagtattct
     4021 cggtatatat gtttcaactt cccgtttact cacgagcaat cgttgttgag tcatcttcgg
     4081 tttttccctt gggatgctga tgttgataag ttcaaggcct ggagacaagg caggaccggt
     4141 tatccgttgg tggatgccgg aatgagagag ctttgggcta ccggatggat gcataacaga
     4201 ataagagtga ttgtttcaag ctttgctgtg aagtttcttc tccttccatg gaaatgggga
     4261 atgaagtatt tctgggatac acttttggat gctgatttgg aatgtgacat ccttggctgg
     4321 cagtatatct ctgggagtat ccccgatggc cacgagcttg atcgcttgga caatcccgcg
     4381 ttacaaggcg ccaaatatga cccagaaggt gagtacataa ggcaatggct tcccgagctt
     4441 gcgagattgc caactgaatg gatccatcat ccatgggacg ctcctttaac cgtactcaaa
     4501 gcttctggtg tggaactcgg aacaaactat gcgaaaccca ttgtagacat cgacacagct
     4561 cgtgagctac tagctaaagc tatttcaaga acccgtgaag cacagatcat gatcggagca
     4621 gcagcccggg atccaccggt cgccaccggt tcagggagcg gatccggctc agggagtgcg
     4681 gccggaactc ccacctgcaa catgcgtgac ggaggcggtc caaaaaagaa gagaaaggta
     4741 tgactgaggc cgcgactcta gagtcgacct gcaggcatgc aagcttgata tcaagcttat
     4801 cgataatcaa cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt
     4861 tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc
     4921 ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga
     4981 gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc
     5041 cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct
     5101 ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg
     5161 gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct
     5221 gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc
     5281 cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg
     5341 tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcatcgata
     5401 ccgtcgacct cgagggaatt aattcgagct cggtaccttt aagaccaatg acttacaagg
     5461 cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact
     5521 cccaacgaag acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct
     5581 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     5641 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     5701 tcagaccctt ttagtcagtg tggaaaatct ctagcagcat ctagaattaa ttccgtgtat
     5761 tctatagtgt cacctaaatc gtatgtgtat gatacataag gttatgtatt aattgtagcc
     5821 gcgttctaac gacaatatgt acaagcctaa ttgtgtagca tctggcttac tgaagcagac
     5881 cctatcatct ctctcgtaaa ctgccgtcag agtcggtttg gttggacgaa ccttctgagt
     5941 ttctggtaac gccgtcccgc acccggaaat ggtcagcgaa ccaatcagca gggtcatcgc
     6001 tagccagatc ctctacgccg gacgcatcgt ggccggcatc accggcgcca caggtgcggt
     6061 tgctggcgcc tatatcgccg acatcaccga tggggaagat cgggctcgcc acttcgggct
     6121 catgagcgct tgtttcggcg tgggtatggt ggcaggcccc gtggccgggg gactgttggg
     6181 cgccatctcc ttgcatgcac cattccttgc ggcggcggtg ctcaacggcc tcaacctact
     6241 actgggctgc ttcctaatgc aggagtcgca taagggagag cgtcgaatgg tgcactctca
     6301 gtacaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca acacccgctg
     6361 acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct
     6421 ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg
     6481 gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt
     6541 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     6601 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     6661 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     6721 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     6781 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     6841 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     6901 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     6961 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     7021 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     7081 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     7141 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     7201 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     7261 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggaa
     7321 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     7381 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     7441 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     7501 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     7561 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     7621 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     7681 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     7741 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     7801 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     7861 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     7921 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     7981 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     8041 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     8101 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     8161 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     8221 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     8281 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     8341 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     8401 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     8461 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     8521 aatgcagctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg
     8581 cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg
     8641 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc
     8701 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
     8761 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt
     8821 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc ttggacacaa
     8881 gacaggcttg cgagatatgt ttgagaatac cactttatcc cgcgtcaggg agaggcagtg
     8941 cgtaaaaaga cgcggactca tgtgaaatac tggtttttag tgcgccagat ctctataatc
     9001 tcgcgcaacc tattttcccc tcgaacactt tttaagccgt agataaacag gctgggacac
     9061 ttcacatgag cgaaaaatac atcgtcacct gggacatgtt gcagatccat gcacgtaaac
     9121 tcgcaagccg actgatgcct tctgaacaat ggaaaggcat tattgccgta agccgtggcg
     9181 gtctgtaccg ggtgcgttac tggcgcgtga actgggtatt cgtcatgtcg ataccgtttg
     9241 tatttccagc tacgatcacg acaaccagcg cgagcttaaa gtgctgaaac gcgcagaagg
     9301 cgatggcgaa ggcttcatcg ttattgatga cctggtggat accggtggta ctgcggttgc
     9361 gattcgtgaa atgtatccaa aagcgcactt tgtcaccatc ttcgcaaaac cggctggtcg
     9421 tccgctggtt gatgactatg ttgttgatat cccgcaagat acctggattg aacagccgtg
     9481 ggatatgggc gtcgtattcg tcccgccaat ctccggtcgc taatcttttc aacgcctggc
     9541 actgccgggc gttgttcttt ttaacttcag gcgggttaca atagtttcca gtaagtattc
     9601 tggaggctgc atccatgaca caggcaaacc tgagcgaaac cctgttcaaa ccccgcttta
     9661 aacatcctga aacctcgacg ctagtccgcc gctttaatca cggcgcacaa ccgcctgtgc
     9721 agtcggccct tgatggtaaa accatccctc actggtatcg catgattaac cgtctgatgt
     9781 ggatctggcg cggcattgac ccacgcgaaa tcctcgacgt ccaggcacgt attgtgatga
     9841 gcgatgccga acgtaccgac gatgatttat acgatacggt gattggctac cgtggcggca
     9901 actggattta tgagtgggcc ccggatcttt gtgaaggaac cttacttctg tggtgtgaca
     9961 taattggaca aactacctac agagatttaa agctctaagg taaatataaa atttttaagt
    10021 gtataatgtg ttaaactact gattctaatt gtttgtgtat tttagattcc aacctatgga
    10081 actgatgaat gggagcagtg gtggaatgcc tttaatgagg aaaacctgtt ttgctcagaa
    10141 gaaatgccat ctagtgatga tgaggctact gctgactctc aacattctac tcctccaaaa
    10201 aagaagagaa aggtagaaga ccccaaggac tttccttcag aattgctaag ttttttgagt
    10261 catgctgtgt ttagtaatag aactcttgct tgctttgcta tttacaccac aaaggaaaaa
    10321 gctgcactgc tatacaagaa aattatggaa aaatattctg taacctttat aagtaggcat
    10381 aacagttata atcataacat actgtttttt cttactccac acaggcatag agtgtctgct
    10441 attaataact atgctcaaaa attgtgtacc tttagctttt taatttgtaa aggggttaat
    10501 aaggaatatt tgatgtatag tgccttgact agagatcata atcagccata ccacatttgt
    10561 agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat
    10621 gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa
    10681 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
    10741 caaactcatc aatgtatctt atcatgtctg gatcaactgg ataactcaag ctaaccaaaa
    10801 tcatcccaaa cttcccaccc cataccctat taccactgcc aattacctgt ggtttcattt
    10861 actctaaacc tgtgattcct ctgaattatt ttcattttaa agaaattgta tttgttaaat
    10921 atgtactaca aacttagtag ttggaagggc taattcactc ccaaagaaga caagatatcc
    10981 ttgatctgtg gatctaccac acacaaggct acttccctga ttagcagaac tacacaccag
    11041 ggccaggggt cagatatcca ctgacctttg gatggtgcta caagctagta ccagttgagc
    11101 cagataaggt agaagaggcc aataaaggag agaacaccag cttgttacac cctgtgagcc
    11161 tgcatgggat ggatgacccg gagagagaag tgttagagtg gaggtttgac agccgcctag
    11221 catttcatca cgtggcccga gagctgcatc cggagtactt caagaactgc tgatatcgag
    11281 cttgctacaa gggactttcc gctggggact ttccagggag gcgtggcctg ggcgggactg
    11341 gggagtggcg agccctcaga tcctgcatat aagcagctgc tttttgcctg tactgggtct
    11401 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
    11461 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
    11521 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
    11581 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
    11641 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
    11701 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtc
//
