LOCUS       sequence-313308-        8650 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector expressing gRNA targeting KAT7 (gRNA ID. A10).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8650)
  AUTHORS   Au YZ, Gu M, De Braekeleer E, Gozdecka M, Aspris D, Tarumoto Y, 
            Cooper J, Yu J, Ong SH, Chen X, Tzelepis K, Huntly BJP, Vassiliou G,
            Yusa K
  TITLE     KAT7 is a genetic vulnerability of acute myeloid leukemias driven by
            MLL rearrangements.
  JOURNAL   Leukemia. 2021 Apr;35(4):1012-1022. doi: 10.1038/s41375-020-1001-z. 
            Epub 2020 Aug 6.
  PUBMED    32764680
REFERENCE   2  (bases 1 to 8650)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41375-020-1001-z"; journalName: "Leukemia"; date: 
            "2021-04"; volume: "35"; issue: "4"; pages: "1012-1022"
COMMENT     Sequence Label: pKLV2-U6gRNA5(gKAT7-A10)-PGKpuro2ABFP-W
FEATURES             Location/Qualifiers
     source          1..8650
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     36..55
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             143..739
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(143..162)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     639..659
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             758..811
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             812..1513
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     primer_bind     complement(1573..1593)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1991..2002)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(2454..2471)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2460..2684
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(2710..2730)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(2710..2728)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2749..2765)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2749..2765)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2762..2784)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2773..2789
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2797..2803,2804..2821,2822..2827))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2842..2863
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2980..2997)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3151..3739)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3231..3250)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3910..4701,4702..4770))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4533..4552
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4771..4875)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(4901..5356)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5038..5059)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5250..5269
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5483..5505
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5497..5514
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5498..5514
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5524..5543
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        5524..5542
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        5858..6056
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6011..6031
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             6074..6254
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6301..6426
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6925..7158
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7343..7387
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7685..7802
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        7877..8125
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     7877..7897
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     8048..8067
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     promoter        join(8254..8650,1..103)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(8289..8312)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
ORIGIN
        1 cggggcgggc gcccgaaggt cctccggagg cccggcattc tgcacgcttc aaaagcgcac
       61 gtctgccgcg ctgttctcct cttcctcatc tccgggcctt tcgacctgca tccatctaga
      121 tctcgagcag ctgaagctta ccatgaccga gtacaagccc acggtgcgcc tcgccacccg
      181 cgacgacgtc cccagggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac
      241 gcgccacacc gtcgatccgg accgccacat cgagcgggtc accgagctgc aagaactctt
      301 cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt
      361 ggcggtctgg accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc
      421 gcgcatggcc gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct
      481 ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga
      541 ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg
      601 cgccggggtg cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg
      661 gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat
      721 gacccgcaag cccggtgccg gcggcgggtc cggaggagag ggcagaggaa gtctcctaac
      781 atgcggtgac gtggaggaga atcctggccc aatgagcgag ctgattaagg agaacatgca
      841 catgaagctg tacatggagg gcaccgtgga caaccatcac ttcaagtgca catccgaggg
      901 cgaaggcaag ccctacgagg gcacccagac catgagaatc aaggtggtcg agggcggccc
      961 tctccccttc gccttcgaca tcctggctac tagcttcctc tacggcagca agaccttcat
     1021 caaccacacc cagggcatcc ccgacttctt caagcagtcc ttccctgagg gcttcacatg
     1081 ggagagagtc accacatacg aggacggggg cgtgctgacc gctacccagg acaccagcct
     1141 ccaggacggc tgcctcatct acaacgtcaa gatcagaggg gtgaacttca catccaacgg
     1201 ccctgtgatg cagaagaaaa cactcggctg ggaggccttc accgagacgc tgtaccccgc
     1261 tgacggcggc ctggaaggca gaaacgacat ggccctgaag ctcgtgggcg ggagccatct
     1321 gatcgcaaac atcaagacca catatagatc caagaaaccc gctaagaacc tcaagatgcc
     1381 tggcgtctac tatgtggact acagactgga aagaatcaag gaggccaaca acgagaccta
     1441 cgtcgagcag cacgaggtgg cagtggccag atactgcgac ctccctagca aactggggca
     1501 caagcttaat tgagcggccg ctcaacctct ggattacaaa atttgtgaaa gattgactgg
     1561 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta
     1621 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct
     1681 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt
     1741 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac
     1801 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg
     1861 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc
     1921 gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg
     1981 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct
     2041 gcggcctctt ccgcgtctac gccttcgccc tcagacgagt cggatctccc tttgggccgc
     2101 ctccccgcag gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt
     2161 tttaaaagaa aaggggggac tggaagggct aattcactcc caaagaagtc aagatctgct
     2221 ttttgcctgt actgggtctc tctggttaga ccagagtctc tctggttaga ccagatctga
     2281 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
     2341 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
     2401 agaccctttt agtcagtgtg gaaaatctct agcagtttaa acccgctgat cagcctcgac
     2461 tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct
     2521 ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct
     2581 gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg
     2641 ggaagtcaat agcaggcatg ctggggatgc ggtgggctct atgggcggcc gttaattaag
     2701 cttttgttcc ctttagtgag ggttaattgc gcgcttggcg taatcatggt catagctgtt
     2761 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg ggagcataaa
     2821 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
     2881 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
     2941 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
     3001 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     3061 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     3121 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     3181 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     3241 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     3301 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     3361 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     3421 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     3481 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     3541 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     3601 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     3661 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     3721 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     3781 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     3841 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     3901 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     3961 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     4021 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     4081 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     4141 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     4201 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     4261 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     4321 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     4381 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     4441 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     4501 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
     4561 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     4621 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     4681 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     4741 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     4801 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     4861 aataggggtt ccgcgcacat ttccccgaaa agtgccacct aaattgtaag cgttaatatt
     4921 ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa
     4981 atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca
     5041 gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc
     5101 gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg
     5161 aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg
     5221 ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg
     5281 gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg
     5341 ccgctacagg gcgcgtccca ttcgccattc aggctgcgca actgttggga agggcgatcg
     5401 gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta
     5461 agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgagcgc
     5521 gcgtaatacg actcactata gggcgaattg actagtacat aacttacggt aaatggcccg
     5581 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata
     5641 gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc
     5701 cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac
     5761 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg
     5821 cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc
     5881 aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc
     5941 aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc
     6001 gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagcgcg
     6061 ttttgcctgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
     6121 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
     6181 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     6241 gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag
     6301 ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga
     6361 ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag
     6421 agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca
     6481 gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga
     6541 ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag
     6601 ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca
     6661 accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag
     6721 atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgg ccgcgctgat
     6781 cttcagacct ggaggaggag atatgaggga caattggaga agtgaattat ataaatataa
     6841 agtagtaaaa attgaaccat taggagtagc acccaccaag gcaaagagaa gagtggtgca
     6901 gagagaaaaa agagcagtgg gaataggagc tttgttcctt gggttcttgg gagcagcagg
     6961 aagcactatg ggcgcagcgt caatgacgct gacggtacag gccagacaat tattgtctgg
     7021 tatagtgcag cagcagaaca atttgctgag ggctattgag gcgcaacagc atctgttgca
     7081 actcacagtc tggggcatca agcagctcca ggcaagaatc ctggctgtgg aaagatacct
     7141 aaaggatcaa cagctcctgg ggatttgggg ttgctctgga aaactcattt gcaccactgc
     7201 tgtgccttgg aatgctagtt ggagtaataa atctctggaa cagatttgga atcacacgac
     7261 ctggatggag tgggacagag aaattaacaa ttacacaagc ttaatacact ccttaattga
     7321 agaatcgcaa aaccagcaag aaaagaatga acaagaatta ttggaattag ataaatgggc
     7381 aagtttgtgg aattggttta acataacaaa ttggctgtgg tatataaaat tattcataat
     7441 gatagtagga ggcttggtag gtttaagaat agtttttgct gtactttcta tagtgaatag
     7501 agttaggcag ggatattcac cattatcgtt tcagacccac ctcccaaccc cgaggggacc
     7561 cgacaggccc gaaggaatag aagaagaagg tggagagaga gacagagaca gatccattcg
     7621 attagtgaac ggatcggcac tgcgtgcgcc aattctgcag acaaatggca gtattcatcc
     7681 acaattttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca
     7741 taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa attcaaaatt
     7801 ttcgggttta ttacagggac agcagagatc cagtttggtt agtaccgggc cctacgcgtc
     7861 caaggtcggg caggaagagg gcctatttcc catgattcct tcatatttgc atatacgata
     7921 caaggctgtt agagagataa ttagaattaa tttgactgta aacacaaaga tattagtaca
     7981 aaatacgtga cgtagaaagt aataatttct tgggtagttt gcagttttaa aattatgttt
     8041 taaaatggac tatcatatgc ttaccgtaac ttgaaagtat ttcgatttct tggctttata
     8101 tatcttgtgg aaaggacgaa acaccgccgc tatgagcttg ataccgttta agagctatgc
     8161 tggaaacagc atagcaagtt taaataaggc tagtccgtta tcaacttgaa aaagtggcac
     8221 cgagtcggtg ctttttttgg atccaattct accgggtagg ggaggcgctt ttcccaaggc
     8281 agtctggagc atgcgcttta gcagccccgc tgggcacttg gcgctacaca agtggcctct
     8341 ggcctcgcac acattccaca tccaccggta ggcgccaacc ggctccgttc tttggtggcc
     8401 ccttcgcgcc accttctact cctcccctag tcaggaagtt cccccccgcc ccgcagctcg
     8461 cgtcgtgcag gacgtgacaa atggaagtag cacgtctcac tagtctcgtg cagatggaca
     8521 gcaccgctga gcaatggaag cgggtaggcc tttggggcag cggccaatag cagctttgct
     8581 ccttcgcttt ctgggctcag aggctgggaa ggggtgggtc cgggggcggg ctcaggggcg
     8641 ggctcagggg
//
