LOCUS       sequence-31332-e         841 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 841)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 841)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: ARFGAP1
FEATURES             Location/Qualifiers
     source          1..841
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             56..73
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             83..100
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             514..531
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(584..602)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      598..645
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(769..788)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 attccctctg aatattttgt ttactttaag aaggagatat accatgggca gcagccatca
       61 tcatcatcat cacagcagcg gcctggttcc gcgtggtagt atggccagcc caagaaccag
      121 gaaggttctt aaagaagtca gggtgcagga tgagaacaac gtttgttttg agtgtggcgc
      181 gttcaatcct cagtgggtca gtgtgaccta cggcatctgg atctgcctgg agtgctcggg
      241 gagacaccgc gggcttgggg ttcacctcag ctttgtgcgc tctgttacta tggacaagtg
      301 gaaggacatt gagcttgaga agatgaaagc tggtgggaat gctaagttcc gagagttcct
      361 ggagtctcag gaggattacg atccttgctg gtccttgcag gagaagtaca acagcagagc
      421 cgcggccctc tttagggata aggtggtcgc tctggccgaa ggcagagagt ggtctctgga
      481 gtcatgatga cgaagcttgc ggccgcactc gagcaccacc accaccacca ctgagatccg
      541 gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta
      601 gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact
      661 atatccggat tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
      721 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt
      781 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc
      841 t
//
