LOCUS       sequence-313320-        2352 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Overexpresses TMC5.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2352)
  TITLE     These plasmids allow lentivirus-mediated overexpression of 
            understudied V5 tagged ion channels in mammalian cell lines.
REFERENCE   2  (bases 1 to 2352)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: IDG_TMC5_OE_1
FEATURES             Location/Qualifiers
     source          1..2352
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(2282..2306)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2308..2349
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atgctgtccg atgaccacgt gaatgaaatc atcatacagg ttgagaatgt ttcctctggg
       61 gtccaaagcc acccatcctc aaatcagatt tttcaagaaa aggtgctgct agactcaagc
      121 atcaacatgg ttttgtcaat atctgacatt gatgtgatag actctcagac agtcagcaaa
      181 aggaatgacc aaaagggtaa ccaggtgctg cggttttcaa catctttgaa tgagtcgatg
      241 tctcagaccc ttcatagcct agaatgcatg ggcatagaca ctcctggttc ttcacatgaa
      301 actgttcaag gacagaagtt aatcgcatcc cttataccca tgacatccag agacagaatt
      361 aaagccatca ggaaccagcc aaggaccatg gaagagaaaa ggaaccttag gaaaatagtt
      421 gacaaagaaa aaagcaaaca gacccatcgt atccttcagc tcaattgctg tattcagtgt
      481 ctgaactcca tttcccgggc ttatcggaga tccaagaaca gcctgtcgga aattctgaat
      541 tccatcagcc tgtggcagaa gacgctgaag atcattggag gcaagtttgg aaccagcgtc
      601 ctctcctatt tcaactttct gagatggctt ttgaagttca acattttctc attcatcctg
      661 aacttcagct tcatcataat ccctcagttt accgtggcca aaaagaacac cctccagttc
      721 actgggctgg agtttttcac tggggtgggt tattttaggg acacagtgat gtactatggc
      781 ttttacacca attccaccat ccagcacggg aacagcgggg catcctacaa catgcagctg
      841 gcctacatct tcacaatcgg agcatgcttg accacctgct tcttcagttt gctgttcagc
      901 atggccaagt atttccggaa caacttcatt aatccccaca tttactccgg agggatcacc
      961 aagctgatct tttgctggga cttcactgtc actcatgaaa aagctgtgaa gctaaaacag
     1021 aagaatctta gcactgagat aagggagaac ctgtcagagc tccgtcagga gaattccaag
     1081 ttgacgttca atcagctgct gacccgcttc tctgcctaca tggtagcctg ggttgtctct
     1141 acaggagtgg ccatagcctg ctgtgcagcc gtttattacc tggctgagta caacttagag
     1201 ttcctgaaga cacacagtaa ccctggggcg gtgctgttac tgcctttcgt tgtgtcctgc
     1261 attaatctgg ccgtgccatg catctactcc atgttcaggc ttgtggagag gtacgagatg
     1321 ccacggcacg aagtctacgt tctcctgatc cgaaacatct ttttgaaaat atcaatcatt
     1381 ggcattcttt gttactattg gctcaacacc gtggccctgt ctggtgaaga gtgttgggaa
     1441 accctcattg gccaggacat ctaccggctc cttctgatgg attttgtgtt ctctttagtc
     1501 aattccttcc tgggggagtt tctgaggaga atcattggga tgcaactgat cacaagtctt
     1561 ggccttcagg agtttgacat tgccaggaac gttctagaac tgatctatgc acaaactctg
     1621 gtgtggattg gcatcttctt ctgccccctg ctgcccttta tccaaatgat tatgcttttc
     1681 atcatgttct actccaaaaa tatcagcctg atgatgaatt tccagcctcc gagcaaagcc
     1741 tggcgggcct cacagatgat gactttcttc atcttcttgc tctttttccc atccttcacc
     1801 ggggtcttgt gcaccctggc catcaccatc tggagattga agccttcagc tgactgtggc
     1861 ccttttcgag gtctgcctct cttcattcac tccatctaca gctggatcga caccctaagt
     1921 acacggcctg gctacctgtg ggttgtttgg atctatcgga acctcattgg aagtgtgcac
     1981 ttctttttca tcctcaccct cattgtgcta atcatcacct atctttactg gcagatcaca
     2041 gagggaagga agattatgat aaggctgctc catgagcaga tcattaatga gggcaaagat
     2101 aaaatgttcc tgatagaaaa attgatcaag ctgcaggata tggagaagaa agcaaacccc
     2161 agctcacttg ttctggaaag gagagaggtg gagcaacaag gctttttgca tttgggggaa
     2221 catgatggca gtcttgactt gcgatctaga agatcagttc aagaaggtaa tccaagggcc
     2281 tgcccaactt tcttgtacaa agtggttggt aagcctatcc ctaaccctct cctcggtctc
     2341 gattctacgt ag
//
