LOCUS       sequence-313324-         717 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Overexpresses SCN3B.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 717)
  TITLE     These plasmids allow lentivirus-mediated overexpression of 
            understudied V5 tagged ion channels in mammalian cell lines.
REFERENCE   2  (bases 1 to 717)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: IDG_SCN3B_OE_1
FEATURES             Location/Qualifiers
     source          1..717
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(4..15)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(647..671)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             673..714
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atgcctgcct tcaatagatt gtttcccctg gcttctctcg tgcttatcta ctgggtcagt
       61 gtctgcttcc ctgtgtgtgt ggaagtgccc tcggagacgg aggccgtgca gggcaacccc
      121 atgaagctgc gctgcatctc ctgcatgaag agagaggagg tggaggccac cacggtggtg
      181 gaatggttct acaggcccga gggcggtaaa gatttcctta tttacgagta tcggaatggc
      241 caccaggagg tggagagccc ctttcagggg cgcctgcagt ggaatggcag caaggacctg
      301 caggacgtgt ccatcactgt gctcaacgtc actctgaacg actctggcct ctacacctgc
      361 aatgtgtccc gggagtttga gtttgaggcg catcggccct ttgtgaagac gacgcggctg
      421 atccccctaa gagtcaccga ggaggctgga gaggacttca cctctgtggt ctcagaaatc
      481 atgatgtaca tccttctggt cttcctcacc ttgtggctgc tcatcgagat gatatattgc
      541 tacagaaagg tctcaaaagc cgaagaggca gcccaagaaa acgcgtctga ctaccttgcc
      601 atcccatctg agaacaagga gaactctgcg gtaccagtgg aggaattgcc aactttcttg
      661 tacaaagtgg ttggtaagcc tatccctaac cctctcctcg gtctcgattc tacgtag
//
