LOCUS       sequence-313327-        1494 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Overexpresses GABRR1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1494)
  TITLE     These plasmids allow lentivirus-mediated overexpression of 
            understudied V5 tagged ion channels in mammalian cell lines.
REFERENCE   2  (bases 1 to 1494)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: IDG_GABRR1_OE_1
FEATURES             Location/Qualifiers
     source          1..1494
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(1424..1448)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1450..1491
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atgagatttg gcatctttct tttgtggtgg ggatgggttt tggccactga aagcagaatg
       61 cactggcccg gaagagaagt ccacgagatg tctaagaaag gcagtaggcc ccaaagacaa
      121 agacgagaag tacatgaaga tgcccacaag caagtcagcc caattctgag acgaagtcct
      181 gacatcacca aatcgcctct gacaaagtca gaacagcttc tgaggataga tgaccatgat
      241 ttcagcatga ggcctggctt tggaggccct gccattcctg ttggtgtgga tgtgcaggtg
      301 gagagtttgg atagcatctc agaggttgac atggacttta cgatgaccct ctacctgagg
      361 cactactgga aggacgagag gctgtctttt ccaagcacca acaacctcag catgacgttt
      421 gatggccggc tggtcaagaa gatctgggtc cctgacatgt ttttcgtgca ctccaaacgc
      481 tccttcatcc acgacaccac cacagacaac gtcatgttgc gggtccagcc tgatgggaaa
      541 gtgctctata gtctcagggt tacagtaact gcaatgtgca acatggactt cagccgattt
      601 cccttggaca cacaaacgtg ctctcttgaa attgaaagct atgcctatac agaagatgac
      661 ctcatgctgt actggaaaaa gggcaatgac tccttaaaga cagatgaacg gatctcactc
      721 tcccagttcc tcattcagga attccacacc accaccaaac tggctttcta cagcagcaca
      781 ggctggtaca accgtctcta cattaatttc acgttgcgtc gccacatctt cttcttcttg
      841 ctccaaactt atttccccgc taccctgatg gtcatgctgt cctgggtgtc cttctggatc
      901 gaccgcagag ccgtgcctgc cagagtcccc ttaggtatca caacggtgct gaccatgtcc
      961 accatcatca cgggcgtgaa tgcctccatg ccgcgcgtct cctacatcaa ggccgtggac
     1021 atctacctct gggtcagctt tgtgttcgtg ttcctctcgg tgctggagta tgcggccgtc
     1081 aactacctga ccactgtgca ggagaggaag gaacagaagc tgcgggagaa gcttccctgc
     1141 accagcggat tacctccgcc ccgcactgca atgctggacg gcaactacag tgatggggag
     1201 gtgaatgacc tggacaacta catgccagag aatggagaga agcccgacag gatgatggtg
     1261 cagctgaccc tggcctcaga gaggagctcc ccacagagga aaagtcagag aagcagctat
     1321 gtgagcatga gaatcgacac ccacgccatt gataaatact ccaggatcat ctttccagca
     1381 gcatacattt tattcaattt aatatactgg tctattttct ccttgccaac tttcttgtac
     1441 aaagtggttg gtaagcctat ccctaaccct ctcctcggtc tcgattctac gtag
//
