LOCUS       sequence-313328-        3369 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Overexpresses TMC3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3369)
  TITLE     These plasmids allow lentivirus-mediated overexpression of 
            understudied V5 tagged ion channels in mammalian cell lines.
REFERENCE   2  (bases 1 to 3369)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: IDG_TMC3_OE_1
FEATURES             Location/Qualifiers
     source          1..3369
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(3299..3323)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3325..3366
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atgaaaacct cgaaggcatc ccagcgctat agaggcatcc ggagaaatgc cagccagtgc
       61 tacctctacc aggaatctct gctgctcagc aacttggatg acagctttag tgctgatgaa
      121 acaggggaca gcaatgatcc ggaacaaatc ttccagaata tacagttcca gaaagatctc
      181 atggcaaaca ttcgctgcag accctggact atgggacaga agctgagggc attgagacaa
      241 gcgaagaaca ttgtgctgaa gtttgaaggg aggctgacca ggacccgagg ctaccaagca
      301 gcaggtgcag agctctggcg gaaatttgct cgtctcgcct gtaactttgt ggtcatcttc
      361 attccctggg aaatgaggat aaagaaaatc gagagtcatt ttggatctgg cgttgcctcc
      421 tatttcatat tcttgagatg gttatttgga attaatattg tgctcaccat tatgacaggt
      481 gcttttattg tcattccaga gctgattgca ggccagccct ttggaagcac agccaggaag
      541 accatcccca aggagcaggt ttcgtctgcc caagacctgg acaccgtctg gtctctgggg
      601 ggctacctcc agtactctgt cctcttctac ggatattacg gcagggagag gaagatcggg
      661 agagctggct accggctgcc cttggcgtat ttcctagtgg ggatggcagt gtttgcttac
      721 agcttcatca ttcttttaaa aaagatggct aagaactccc gcacgagtct tgccagtgct
      781 tccaatgaaa actatacctt ctgctggcgg gtgttctgtg cctgggatta cctcattgga
      841 aacccagagg ctgcagagag caaaacagct gccatagtga acagcatcag ggaagctata
      901 ttggaagaac aggagaaaaa gaaaagcaaa aacctggcag tgaccatctg cctgaggatt
      961 attgccaaca tcctggtgct tctctcactg gctgggagca tttatctcat ctactttgtg
     1021 gtggaccggt cccagaagct ggagcagtcg aagaaggagc tgacactttg ggaaaagaat
     1081 gaggtcagtg tggtggtctc cctcgtcacc atgatagcac catcagcctt tgacctcatt
     1141 gctgccttag agatgtacca ccccaggacc acgctgcgct tccagcttgc aagggtcctt
     1201 gtgctgtatt tgggaaatct ctacagcctg atcattgctc tcctggacaa agttaacagc
     1261 atgagcattg aggaaatggc taccaaaaat aacacaagtc attggataga ttccaccacc
     1321 ttctttgcaa ccaggacagc tcctgaagaa gagaaatggt ccacatctcg gcctggaatg
     1381 gggctcagga gaaacaacac atgggccttg gaagagacca gcatttcagc ttataccatg
     1441 cctcttataa aggccaacaa gactagcctc cacactcaga gtccacaaga ccagtgctgg
     1501 gagacatacg ttggtcagga gatgctgaag ctctccatca ttgacatgct cttcaccgtg
     1561 gcgagcattc tgctcataga cttcttccga ggacttttcg tgcggtactt aagtgactac
     1621 tggtgttggg atctggaaag caagtttcct gaatatggag aattcaaaat agctgagaat
     1681 gtgctacatt tagtctacaa ccaaggaatg atttggatgg gggccttctt ctccccatgt
     1741 ctcccagcgt tcaacgttct caaactcatt gggctcatgt acctgagaag ctgggctgtg
     1801 ctgacctgca atgtgcccca ccagcaagta tttcgagcct caagatccaa caacttctac
     1861 ttggcaatgc tcctgtttat gctgtttctg tgcatgctgc caaccatttt tgctattgtc
     1921 cgatacaagc catctctaaa ctgtgggcca ttcagtggac aagagaaaat ttatgacatt
     1981 gtgtcagaaa cgattgagaa agactttcct gtgtggtttg gctccgtggt tggacacatc
     2041 agtagccccg tggtcatcct gcccgcagta ctcctgcttt tcatgctcat ctattatctc
     2101 cagagcatcg cacggtctct aaagctcagc aaccaccagc tcaaaatgca gatccaaaac
     2161 gcaagatcag aggataagaa aaaggttgcc cagatggtag aagcccgaat ccagacccag
     2221 gaagaaagca ccaagaagct tccaaacgac agtgatctta ccagccagct gtcctcagcg
     2281 cactcgggca caccccaaaa caacggcaac gtggcccatt ttgactcagg gagcagcaag
     2341 agtggcagga tagagacagt cgcacagtcc atgccccaga gcccgaggcc aggggacagg
     2401 gctcctagct cacctctccc tggggtcccc aaatccaggc tagagcatga aactaacagg
     2461 tatctgcacg gtctgtgtgc aagcaccagt gaccttcaca ggaacagatc gcgcacacct
     2521 atgacattta caacgcacat cgaagatgta cactcagaac ctcttttccg aaaagacttt
     2581 cagcaaatca accctcctca ccgtggacca caggcctcga ctttgctggc acagggtccc
     2641 aggccccacg cccccagata ctatgtcatt aatgaatatg actcttacaa gaaaaaacat
     2701 ctaaatgtct ggccagaaag acatttcaag atagatgctt caggtgacat tgtggagctg
     2761 taccccagga acgtgcgaca atatgcatcc cgggtccccc gccagcctcc ctccccacag
     2821 ctgagtgaag aagaggagga gacgccaagc agagattgga tcaaacggtc tcttccccca
     2881 cgctccctga tagacctccg tcgggctcct catttttata ttggggagag gtccgaaagt
     2941 cagacccggg atcccgagca ccaggggagg gtgcactaca agtcgtggaa tgaagatttt
     3001 gagggtcacc ttgagaggcc agcctatgtg cccagaaagc cacgatcccg gaatttccaa
     3061 tacccacagc cccctctgaa gcccagaggg aagcccaggt tcgagccatc cctcacggaa
     3121 tctgactccg tgtcggcagc atccagcagt gaccagcaga acagcagcgc tgaccagtac
     3181 ctgcaggtca cccacagcca gggcaggttc ccgaggtccg tgggccagcc cagcaggagg
     3241 aaggccaagt cggggcagga gctgaccgtg gatctggatg acttgatttg ttcaaatgtc
     3301 acagctttct tgtacaaagt ggttggtaag cctatcccta accctctcct cggtctcgat
     3361 tctacgtag
//
