LOCUS       sequence-313330-        2127 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Overexpresses CLCNKA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2127)
  TITLE     These plasmids allow lentivirus-mediated overexpression of 
            understudied V5 tagged ion channels in mammalian cell lines.
REFERENCE   2  (bases 1 to 2127)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: IDG_CLCNKA_OE_1
FEATURES             Location/Qualifiers
     source          1..2127
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(2057..2081)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2083..2124
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atggaggagt tggtggggct gcgtgagggc ttctcagggg accctgtgac tctgcaggag
       61 ctgtggggcc cctgtcccca catccgccga gccatccaag gtggcctgga gtggctaaag
      121 cagaaggtgt tccgcctggg agaagactgg tacttcctga tgaccctcgg ggtgctcatg
      181 gccctggtca gctatgccat gaactttgcc atcgggtgtg tggtccgagc acaccagtgg
      241 ctgtacaggg agattgggga cagccacctg ctccggtatc tttcctggac tgtgtaccct
      301 gtggccctcg tctctttctc ctcaggcttc tcccagagca tcacgccctc ctctggaggt
      361 tctggaatcc cggagctgaa gaccatgttg gcgggtgtga tcttggagga ctacctggat
      421 atcaagaact ttggggccaa ggtggtgggc ctctcctgca ccctggccac cggcagcacc
      481 ctgttcctgg gcaaagtggg ccctttcgtg cacctgtctg taatgatcgc tgcctacctg
      541 ggccgtgtgc gcaccacgac catcggggag cctgagaaca agagcaagca aaacgaaatg
      601 ctggtggcag cggcggcagt gggcgtggcc acagtctttg cagctccctt cagcggcgtc
      661 ctgttcagca tcgaggtcat gtcttcccac ttctctgtcc gggattactg gaggggcttc
      721 tttgcggcca cctgcggggc cttcatattc cggctcctgg cagtcttcaa cagcgagcag
      781 gagaccatca cctccctcta caagaccagt ttccgggtgg acgttccctt cgacctgcct
      841 gagatcttct tttttgtggc gctgggtggc atctgcggcg tcctgagctg tgcttacctc
      901 ttctgtcagc gaaccttcct cagcttcatc aagaccaatc ggttcagctc caaactgctg
      961 gctactagca agcctgtgta ctccgctctg gccaccttgc ttctcgcctc catcacctac
     1021 ccgcctggtg tgggccactt cctagcttct cggctgtcca tgaagcagca tctggactcg
     1081 ctgttcgaca accactcctg ggcgctgatg acccagaact ccagcccacc ctggcccgag
     1141 gagctcgacc cccagcacct ttggtgggaa tggtaccacc cgcggttcac catctttggg
     1201 acccttgcct tcttcctggt tatgaagttc tggatgctga ttctggccac caccatcccc
     1261 atgcctgccg ggtacttcat gcccatcttt atccttggag ctgccatcgg gcgcctcttg
     1321 ggagaggctc ttgccgtcgc cttccctgag ggcattgtga ctggaggggt taccaatccc
     1381 atcatgcccg gggggtatgc tctggcaggg gctgcagcct tctcaggggc tgtgacccac
     1441 accatctcca cggcgctgct ggcctttgag ctgaccggcc agatagtgca tgcactgccc
     1501 gtgctgatgg cggtgctggc agccaacgcc attgcacaga gctgccagcc ctccttctat
     1561 gatggcacca tcattgtcaa gaagctgcca tacctgccac ggattctggg ccgcaacatc
     1621 ggctcccacc atgtgagggt ggagcacttc atgaaccaca gcatcaccac actggccaag
     1681 gacacgccgc tggaggaggt ggtcaaggtt gtgacctcca cagacgtgac cgagtatccc
     1741 ctggtggaga gcacagagtc ccagatcctg gtaggcatcg tgcagagggc ccagctggtg
     1801 caggccctcc aggctgagcc tccttccagg gctccaggac accagtgtct ccaggacatc
     1861 ttggccaggg gctgccccac ggaaccagtg accctgacgc tattctcaga gaccaccttg
     1921 caccaggcac aaaacctctt taagctgttg aaccttcagt ccctcttcgt gacatcgcgg
     1981 ggcagagctg tgggctgcgt gtcctgggtg gagatgaaga aagcaatttc caacctgaca
     2041 aatccgccag ctccaaagac agctttcttg tacaaagtgg ttggtaagcc tatccctaac
     2101 cctctcctcg gtctcgattc tacgtag
//
