LOCUS       sequence-313335-        2013 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Overexpresses ASIC4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2013)
  TITLE     These plasmids allow lentivirus-mediated overexpression of 
            understudied V5 tagged ion channels in mammalian cell lines.
REFERENCE   2  (bases 1 to 2013)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: IDG_ASIC4_OE_1
FEATURES             Location/Qualifiers
     source          1..2013
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(1943..1967)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1969..2010
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atgctgagcg gagcggctgg ggctgcgcgg cgtggcggag cagcgctcgc tccctcgctc
       61 actcgctcgc tcgcagggac acacgcaggg gctgacagct gtgctggtgc tgataaggga
      121 agccacaagg agacgatcga ggagagagac aagcggcagc agaggcagca gcggcagagg
      181 cagcaccagg gctgcggagc tgctgggagt gggagtgact cccccacctc gggcccccac
      241 cctgtccctg tcctcttccc gcttgccctg agtttagaag agcagccgct gccaccactg
      301 ccactcggga gggcaccagg gctgctggct agggagggac agggcaggga ggctctggcc
      361 agtcccagca gccggggaca gatgccgatc gagattgtgt gcaaaatcaa atttgctgag
      421 gaggatgcga aacccaagga gaaggaggca ggggatgagc agagcctcct cggggctgtt
      481 gcccctggag cagccccccg agacctggcc acctttgcca gcaccagcac cctgcatgga
      541 ctgggccggg cctgtggccc aggcccccac ggactgcgca gaaccctgtg ggcactggcc
      601 ctactcacct cgctggctgc cttcctgtac caggcggctg gcctggcccg gggctacctg
      661 acccggcctc acctggtggc aatggacccc gctgccccag ccccagtggc gggcttcccg
      721 gctgtcaccc tctgcaatat caaccgcttc cggcattcgg cactcagcga tgccgacatc
      781 ttccacctgg ccaatctgac agggctgccc cccaaagacc gggatgggca ccgtgcggct
      841 ggcctgcgct acccagagcc tgacatggta gacatcctca accgcactgg ccaccagctc
      901 gccgacatgc ttaagagctg caacttcagt gggcatcact gctccgccag caacttctct
      961 gtggtctata ctcgctatgg gaagtgttac accttcaacg cggacccgcg gagctcgctg
     1021 cccagccggg cagggggcat gggcagtggc ctggagatca tgctggacat ccagcaggag
     1081 gagtacctgc ccatctggag ggagacaaat gagacgtcgt ttgaggcagg tattcgggtg
     1141 cagatccaca gccaggagga gccgccctac atccaccagc tggggttcgg ggtgtcccca
     1201 ggcttccaga cctttgtgtc ctgccaggaa cagcggctga cctacctgcc ccagccctgg
     1261 ggcaactgcc gcgcagagag tgagctcagg gagcctgagc ttcagggcta ctcggcctac
     1321 agtgtgtctg cctgccggct gcgctgtgaa aaggaggccg tgcttcagcg ctgccactgc
     1381 cggatggtgc acatgccaga ctccctgggt gggggccctg agggcccgtg cttctgcccc
     1441 accccctgca acctgacacg ctatgggaaa gagatctcca tggtcaggat ccccaacagg
     1501 ggctcagccc ggtacctggc gaggaagtac aaccgcaacg agacctacat acgggagaac
     1561 ttcctggtcc tagatgtctt ctttgaggcc ctgacctctg aagccatgga gcagcgagca
     1621 gcctatggcc tgtcagccct gctgggagac ctcgggggac agatgggcct gttcattggg
     1681 gccagcatcc tcacgttgct ggagatcctc gactacatct atgaggtgtc ctgggatcga
     1741 ctgaagcggg tatggaggcg tcccaagacc cccctgcgga cctccactgg gggcatctcc
     1801 actttggggc ttcaggagct gaaggaacag agtccctgcc cgagcctggg ccgagcggag
     1861 ggtggggggg tcagcagtct gctccccaat caccaccacc cccacggtcc cccaggaggt
     1921 ctctttgaag attttgcttg ctacccagct ttcttgtaca aagtggttgg taagcctatc
     1981 cctaaccctc tcctcggtct cgattctacg tag
//
