LOCUS       sequence-313339-        2013 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Overexpresses KCND1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2013)
  TITLE     These plasmids allow lentivirus-mediated overexpression of 
            understudied V5 tagged ion channels in mammalian cell lines.
REFERENCE   2  (bases 1 to 2013)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: IDG_KCND1_OE_1
FEATURES             Location/Qualifiers
     source          1..2013
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             982..993
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     protein_bind    complement(1943..1967)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1969..2010
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atggcggcag gcctggccac gtggctgcct tttgctcggg cagcagcagt gggctggctg
       61 cccctggccc agcaacccct gcccccggca ccgggggtga aggcatctcg aggagatgag
      121 gttctggtgg tgaacgtgag cggacggcgc tttgagactt ggaagaatac gctggaccgc
      181 tacccagaca ccttgctggg cagctcggag aaggaattct tctacgatgc tgactcaggc
      241 gagtacttct tcgatcgcga ccctgacatg ttccgccatg tgctgaactt ctaccgaacg
      301 gggcggctgc attgcccacg gcaggagtgc atccaggcct tcgacgaaga gctggctttc
      361 tacggcctgg ttcccgagct agtcggtgac tgctgccttg aagagtatcg ggaccgaaag
      421 aaggagaatg ccgagcgcct ggcagaggat gaggaggcag agcaggccgg ggacggccca
      481 gccctgccag caggcagctc cctgcggcag cggctctggc gggccttcga gaatccacac
      541 acgagcaccg cagccctcgt tttctactat gtgaccggct tcttcatcgc cgtgtcggtc
      601 atcgccaatg tggtggagac catcccatgc cgcggctctg cacgcaggtc ctcaagggag
      661 cagccctgtg gcgaacgctt cccacaggcc tttttctgca tggacacagc ctgtgtactc
      721 atattcacag gtgaatacct cctgcggctg tttgccgccc ccagccgttg ccgcttcctg
      781 cggagtgtca tgagcctcat cgacgtggtg gccatcctgc cctactacat tgggcttttg
      841 gtgcccaaga acgacgatgt ctctggcgcc tttgtcaccc tgcgtgtgtt ccgggtgttt
      901 cgcatcttca agttctccag gcactcacag ggcttgagga ttctgggcta cacactcaag
      961 agctgtgcct ctgagctggg ctttctcctc ttttccctaa ccatggccat catcatcttt
     1021 gccactgtca tgttttatgc tgagaagggc acaaacaaga ccaactttac aagcatccct
     1081 gcggccttct ggtataccat tgtcaccatg accacgcttg gctacggaga catggtgccc
     1141 agcaccattg ctggcaagat tttcgggtcc atctgctcac tcagtggcgt cttggtcatt
     1201 gccctgcctg tgccagtcat tgtgtccaac tttagccgca tctaccacca gaaccagcgg
     1261 gctgacaagc gccgagcaca gcagaaggtg cgcttggcaa ggatccgatt ggcaaagagt
     1321 ggtaccacca atgccttcct gcagtacaag cagaatgggg gccttgagga cagcggcagt
     1381 ggcgaggaac aggctctttg tgtcaggaac cgttctgcct ttgaacagca acatcaccac
     1441 ttgctgcact gtctagagaa gacaacgtgc catgagttca cagatgagct caccttcagt
     1501 gaagccctgg gagccgtctc gccgggtggc cgcaccagcc gtagcacctc tgtgtcttcc
     1561 cagccagtgg gacccggaag cctgctgtct tcttgctgcc ctcgcagggc caagcgccgc
     1621 gccatccgcc ttgccaactc cactgcctca gtcagccgtg gcagcatgca ggagctggac
     1681 atgctggcag ggctgcgcag gagccatgcc cctcagagcc gctccagcct caatgccaag
     1741 ccccatgaca gccttgacct gaactgcgac agccgggact tcgtggctgc cattatcagc
     1801 atccctaccc ctcctgccaa caccccagat gagagccaac cttcctcccc tggcggcggt
     1861 ggcagggccg gcagcaccct caggaactcc agcctgggta ccccttgcct cttccccgag
     1921 actgtcaaga tctcatccct gtgcccaact ttcttgtaca aagtggttgg taagcctatc
     1981 cctaaccctc tcctcggtct cgattctacg tag
//
