LOCUS       sequence-313344-        2901 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Overexpresses CLCA2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2901)
  TITLE     These plasmids allow lentivirus-mediated overexpression of 
            understudied V5 tagged ion channels in mammalian cell lines.
REFERENCE   2  (bases 1 to 2901)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: IDG_CLCA2_OE_1
FEATURES             Location/Qualifiers
     source          1..2901
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(2831..2855)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2857..2898
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atgacccaaa ggagcattgc aggtcctatt tgcaacctga agtttgtgac tctcctggtt
       61 gccttaagtt cagaactccc attcctggga gctggagtac agcttcaaga caatgggtat
      121 aatggattgc tcattgcaat taatcctcag gtacctgaga atcagaacct catctcaaac
      181 attaaggaaa tgataactga agcttcattt tacctattta atgctaccaa gagaagagta
      241 tttttcagaa atataaagat tttaatacct gccacatgga aagctaataa taacagcaaa
      301 ataaaacaag aatcatatga aaaggcaaat gtcatagtga ctgactggta tggggcacat
      361 ggagatgatc catacaccct acaatacaga gggtgtggaa aagagggaaa atacattcat
      421 ttcacaccta atttcctact gaatgataac ttaacagctg gctacggatc acgaggccga
      481 gtgtttgtcc atgaatgggc ccacctccgt tggggtgtgt tcgatgagta taacaatgac
      541 aaacctttct acataaatgg gcaaaatcaa attaaagtga caaggtgttc atctgacatc
      601 acaggcattt ttgtgtgtga aaaaggtcct tgcccccaag aaaactgtat tattagtaag
      661 ctttttaaag aaggatgcac ctttatctac aatagcaccc aaaatgcaac tgcatcaata
      721 atgttcatgc aaagtttatc ttctgtggtt gaattttgta atgcaagtac ccacaaccaa
      781 gaagcaccaa acctacagaa ccagatgtgc agcctcagaa gtgcatggga tgtaatcaca
      841 gactctgctg actttcacca cagctttccc atgaatggga ctgagcttcc acctcctccc
      901 acattctcgc ttgtacaggc tggtgacaaa gtggtctgtt tagtgctgga tgtgtccagc
      961 aagatggcag aggctgacag actccttcaa ctacaacaag ccgcagaatt ttatttgatg
     1021 cagattgttg aaattcatac cttcgtgggc attgccagtt tcgacagcaa aggagagatc
     1081 agagcccagc tacaccaaat taacagcaat gatgatcgaa agttgctggt ttcatatctg
     1141 cccaccactg tatcagctaa aacagacatc agcatttgtt cagggcttaa gaaaggattt
     1201 gaggtggttg aaaaactgaa tggaaaagct tatggctctg tgatgatatt agtgaccagc
     1261 ggagatgata agcttcttgg caattgctta cccactgtgc tcagcagtgg ttcaacaatt
     1321 cactccattg ccctgggttc atctgcagcc ccaaatctgg aggaattatc acgtcttaca
     1381 ggaggtttaa agttctttgt tccagatata tcaaactcca atagcatgat tgatgctttc
     1441 agtagaattt cctctggaac tggagacatt ttccagcaac atattcagct tgaaagtaca
     1501 ggtgaaaatg tcaaacctca ccatcaattg aaaaacacag tgactgtgga taatactgtg
     1561 ggcaacgaca ctatgtttct agttacgtgg caggccagtg gtcctcctga gattatatta
     1621 tttgatcctg atggacgaaa atactacaca aataatttta tcaccaatct aacttttcgg
     1681 acagctagtc tttggattcc aggaacagct aagcctgggc actggactta caccctgaac
     1741 aatacccatc attctctgca agccctgaaa gtgacagtga cctctcgcgc ctccaactca
     1801 gctgtgcccc cagccactgt ggaagccttt gtggaaagag acagcctcca ttttcctcat
     1861 cctgtgatga tttatgccaa tgtgaaacag ggattttatc ccattcttaa tgccactgtc
     1921 actgccacag ttgagccaga gactggagat cctgttacgc tgagactcct tgatgatgga
     1981 gcaggtgctg atgttataaa aaatgatgga atttactcga ggtatttttt ctcctttgct
     2041 gcaaatggta gatatagctt gaaagtgcat gtcaatcact ctcccagcat aagcacccca
     2101 gcccactcta ttccagggag tcatgctatg tatgtaccag gttacacagc aaacggtaat
     2161 attcagatga atgctccaag gaaatcagta ggcagaaatg aggaggagcg aaagtggggc
     2221 tttagccgag tcagctcagg aggctccttt tcagtgctgg gagttccagc tggcccccac
     2281 cctgatgtgt ttccaccatg caaaattatt gacctggaag ctgtaaaagt agaagaggaa
     2341 ttgaccctat cttggacagc acctggagaa gactttgatc agggccaggc tacaagctat
     2401 gaaataagaa tgagtaaaag tctacagaat atccaagatg actttaacaa tgctatttta
     2461 gtaaatacat caaagcgaaa tcctcagcaa gctggcatca gggagatatt tacgttctca
     2521 ccccaaattt ccacgaatgg acctgaacat cagccaaatg gagaaacaca tgaaagccac
     2581 agaatttatg ttgcaatacg agcaatggat aggaactcct tacagtctgc tgtatctaac
     2641 attgcccagg cgcctctgtt tattcccccc aattctgatc ctgtacctgc cagagattat
     2701 cttatattga aaggagtttt aacagcaatg ggtttgatag gaatcatttg ccttattata
     2761 gttgtgacac atcatacttt aagcaggaaa aagagagcag acaagaaaga gaatggaaca
     2821 aaattattat acccaacttt cttgtacaaa gtggttggta agcctatccc taaccctctc
     2881 ctcggtctcg attctacgta g
//
