LOCUS       sequence-313347-        1656 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Overexpresses CHRNA2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1656)
  TITLE     These plasmids allow lentivirus-mediated overexpression of 
            understudied V5 tagged ion channels in mammalian cell lines.
REFERENCE   2  (bases 1 to 1656)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: IDG_CHRNA2_OE_1
FEATURES             Location/Qualifiers
     source          1..1656
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(1586..1610)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1612..1653
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atgggcccct cctgtcctgt gttcctgtcc ttcacaaagc tcagcctgtg gtggctcctt
       61 ctgaccccag caggtggaga ggaagctaag cgcccacctc ccagggctcc tggagaccca
      121 ctctcctctc ccagtcccac ggcattgccg cagggaggct cgcataccga gactgaggac
      181 cggctcttca aacacctctt ccggggctac aaccgctggg cgcgcccggt gcccaacact
      241 tcagacgtgg tgattgtgcg ctttggactg tccatcgctc agctcatcga tgtggatgag
      301 aagaaccaaa tgatgaccac caacgtctgg ctaaaacagg agtggagcga ctacaaactg
      361 cgctggaacc ccactgattt tggcaacatc acatctctca gggtcccttc tgagatgatc
      421 tggatccccg acattgttct ctacaacaat gcagatgggg agtttgcagt gacccacatg
      481 accaaggccc acctcttctc cacgggcact gtgcactggg tgcccccggc catctacaag
      541 agctcctgca gcatcgacgt caccttcttc cccttcgacc agcagaactg caagatgaag
      601 tttggctcct ggacttatga caaggccaag atcgacctgg agcagatgga gcagactgtg
      661 gacctgaagg actactggga gagcggcgag tgggccatcg tcaatgccac gggcacctac
      721 aacagcaaga agtacgactg ctgcgccgag atctaccccg acgtcaccta cgccttcgtc
      781 atccggcggc tgccgctctt ctacaccatc aacctcatca tcccctgcct gctcatctcc
      841 tgcctcactg tgctggtctt ctacctgccc tccgactgcg gcgagaagat cacgctgtgc
      901 atttcggtgc tgctgtcact caccgtcttc ctgctgctca tcactgagat catcccgtcc
      961 acctcgctgg tcatcccgct catcggcgag tacctgctgt tcaccatgat cttcgtcacc
     1021 ctgtccatcg tcatcaccgt cttcgtgctc aatgtgcacc accgctcccc cagcacccac
     1081 accatgcccc actgggtgcg gggggccctt ctgggctgtg tgccccggtg gcttctgatg
     1141 aaccggcccc caccacccgt ggagctctgc caccccctac gcctgaagct cagcccctct
     1201 tatcactggc tggagagcaa cgtggatgcc gaggagaggg aggtggtggt ggaggaggag
     1261 gacagatggg catgtgcagg tcatgtggcc ccctctgtgg gcaccctctg cagccacggc
     1321 cacctgcact ctggggcctc aggtcccaag gctgaggctc tgctgcagga gggtgagctg
     1381 ctgctatcac cccacatgca gaaggcactg gaaggtgtgc actacattgc cgaccacctg
     1441 cggtctgagg atgctgactc ttcggtgaag gaggactgga agtatgttgc catggtcatc
     1501 gacaggatct tcctctggct gtttatcatc gtctgcttcc tggggaccat cggcctcttt
     1561 ctgcctccgt tcctagctgg aatgatcaca gctttcttgt acaaagtggt tggtaagcct
     1621 atccctaacc ctctcctcgg tctcgattct acgtag
//
