LOCUS       sequence-313358-        1575 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Overexpresses CHRNB1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1575)
  TITLE     These plasmids allow lentivirus-mediated overexpression of 
            understudied V5 tagged ion channels in mammalian cell lines.
REFERENCE   2  (bases 1 to 1575)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: IDG_CHRNB1_OE_1
FEATURES             Location/Qualifiers
     source          1..1575
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(1505..1529)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1531..1572
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atgaccccag gggctctgct gatgctgctg ggggcgctgg gggcgccgct cgccccaggc
       61 gtccgcggct cggaggcgga gggtcgactc cgggagaaac ttttctctgg ctatgatagc
      121 tccgtgcggc cagcgcggga ggtgggagac cgtgtcaggg tcagcgttgg tctcatcctg
      181 gcgcaactca tcagcctgaa cgagaaggat gaagagatga gcacaaaggt gtacttagac
      241 ctggagtgga ctgactacag gctgagctgg gaccctgcgg agcacgacgg catcgattcg
      301 ctccgcatca cggcggaatc cgtgtggctc cctgacgtgg tgctactgaa caacaatgat
      361 gggaattttg acgtggctct ggacattagc gtcgtggtgt cctccgacgg ctccgtgcgt
      421 tggcaacccc cgggcatcta tcgcagcagc tgcagcatcc aggtcaccta cttccccttc
      481 gactggcaga attgcactat ggtgttcagc tcctacagct acgacagctc ggaggtcagc
      541 ctgcagacag gcctgggtcc tgacgggcaa gggcatcagg aaatccacat tcatgaaggg
      601 actttcattg agaatggcca gtgggagatt atccacaagc cctctcggct aatccagcct
      661 ccaggcgatc ctaggggagg gagggaagga cagcgccagg aagtcatctt ctacctcatc
      721 atccgccgca agcctctctt ctacctggtc aacgtcattg ccccatgcat cctcatcact
      781 cttctggcca tcttcgtctt ctacctgcca ccagatgcag gagagaagat ggggctctca
      841 atctttgccc tgctgaccct tactgtgttc ctgctgctgc tggctgacaa agtacctgag
      901 acctcactat cagtacccat tattatcaag tacctcatgt ttaccatggt cctcgtcacc
      961 ttctcagtca tccttagtgt cgtggttctc aacctgcacc accgctcacc ccacacccac
     1021 caaatgcccc tttgggtccg tcagatcttc attcacaaac ttccgctgta cctgcgtcta
     1081 aaaaggccca aacccgagag agacctgatg ccggagcccc ctcactgttc ttctccagga
     1141 agtggctggg gtcggggaac agatgaatat ttcatccgga agccgccaag tgattttctc
     1201 ttccccaaac ccaataggtt ccagcctgaa ctgtctgccc ctgatctgcg gcgatttatc
     1261 gatggtccaa accgggctgt ggccctgctt ccggagctac gggaggtcgt ctcctctatc
     1321 agctacatcg ctcgacagct gcaggaacag gaggaccacg atgcgctgaa ggaggactgg
     1381 cagtttgtgg ccatggtagt ggaccgcctc ttcctgtgga ctttcatcat cttcaccagc
     1441 gttgggaccc tagtcatctt cctggacgcc acgtaccact tgccccctcc agaccccttt
     1501 ccttgcccaa ctttcttgta caaagtggtt ggtaagccta tccctaaccc tctcctcggt
     1561 ctcgattcta cgtag
//
