LOCUS       sequence-313359-        1848 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Overexpresses CACNB1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1848)
  TITLE     These plasmids allow lentivirus-mediated overexpression of 
            understudied V5 tagged ion channels in mammalian cell lines.
REFERENCE   2  (bases 1 to 1848)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: IDG_CACNB1_OE_1
FEATURES             Location/Qualifiers
     source          1..1848
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(1778..1802)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1804..1845
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atgtcccggg gcccttaccc accctcccag gagatcccca tggaggtctt cgaccccagc
       61 ccgcagggca aatacagcaa gaggaaaggg cgattcaaac ggtcagatgg gagcacgtcc
      121 tcggatacca catccaacag ctttgtccgc cagggctcag cggagtccta caccagccgt
      181 ccatcagact ctgatgtatc tctggaggag gaccgggaag ccttaaggaa ggaagcagag
      241 cgccaggcat tagcgcagct cgagaaggcc aagaccaagc cagtggcatt tgctgtgcgg
      301 acaaatgttg gctacaatcc gtctccaggg gatgaggtgc ctgtgcaggg agtggccatc
      361 accttcgagc ccaaagactt cctgcacatc aaggagaaat acaataatga ctggtggatc
      421 gggcggctgg tgaaggaggg ctgtgaggtt ggcttcattc ccagccccgt caaactggac
      481 agccttcgcc tgctgcagga acagaagctg cgccagaacc gcctcggctc cagcaaatca
      541 ggcgataact ccagttccag tctgggagat gtggtgactg gcacccgccg ccccacaccc
      601 cctgccagtg ccaaacagaa gcagaagtcg acagagcatg tgccccccta tgacgtggtg
      661 ccttccatga ggcccatcat cctggtggga ccgtcgctca agggctacga ggttacagac
      721 atgatgcaga aagctttatt tgacttcttg aagcatcggt ttgatggcag gacctccatc
      781 actcgtgtga cggcagatat ttccctggct aagcgctcag ttctcaacaa ccccagcaaa
      841 cacatcatca ttgagcgctc caacacacgc tccagcctgg ctgaggtgca gagtgaaatc
      901 gagcgaatct tcgagctggc ccggaccctt cagttggtcg ctctggatgc tgacaccatc
      961 aatcacccag cccagctgtc caagacctcg ctggccccca tcattgttta catcaagatc
     1021 acctctccca aggtacttca aaggctcatc aagtcccgag gaaagtctca gtccaaacac
     1081 ctcaatgtcc aaatagcggc ctcggaaaag ctggcacagt gccaccctga aatgtttgac
     1141 atcatcctgg atgagaacca attggaggat gcctgcgagc atctggcgga gtacttggaa
     1201 gcctattgga aggccacaca cccgcccagc agcacgccac ccaatccgct gctgaaccgc
     1261 accatggcta ccgcagccct ggctgccagc cctgcccctg tctccaacct ccagggaccc
     1321 taccttgctt ccggggacca gccactggaa cgggccaccg gggagcacgc cagcatgcac
     1381 gagtacccag gggagctggg ccagccccca ggcctttacc ccagcagcca cccaccaggc
     1441 cgggcaggca cgctacgggc actgtcccgc caagacactt ttgatgccga cacccccggc
     1501 agccgaaact ctgcctacac ggagctggga gactcatgtg tggacatgga gactgacccc
     1561 tcagaggggc cagggcttgg agaccctgca gggggcggca cgcccccagc ccgacaggga
     1621 tcctgggagg acgaggaaga agactatgag gaagagctga ccgacaaccg gaaccggggc
     1681 cggaataagg cccgctactg cgctgagggt gggggtccag ttttggggcg caacaagaat
     1741 gagctggagg gctggggacg aggcgtctac attcgctgcc caactttctt gtacaaagtg
     1801 gttggtaagc ctatccctaa ccctctcctc ggtctcgatt ctacgtag
//
