LOCUS       sequence-31336-e         902 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 902)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 902)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: NRASA
FEATURES             Location/Qualifiers
     source          1..902
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             42..59
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             72..92
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             638..655
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(708..726)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      722..769
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 ctctgaatat tttgtttact ttaagaagga gatataccat gcatcatcat catcatcaca
       61 gcagcggcag agaaaacttg tatttccagg gcatgactga gtacaaactg gtggtggttg
      121 gagcaggtgg tgttgggaaa agcgcactga caatccagct aatccagaac cactttgtag
      181 atgaatatga tcccaccata gaggattctt acagaaaaca agtggttata gatggtgaaa
      241 cctgtttgtt ggacatactg gatacagctg gacaagaaga gtacagtgcc atgagagacc
      301 aatacatgag gacaggcgaa ggcttcctct gtgtatttgc catcaataat agcaagtcat
      361 ttgcggatat taacctctac agggagcaga ttaagcgagt aaaagactcg gatgatgtac
      421 ctatggtgct agtgggaaac aagtgtgatt tgccaacaag gacagttgat acaaaacaag
      481 cccacgaact ggccaagagt tacgggattc cattcattga aacctcagcc aagaccagac
      541 agggtgttga agatgctttt tacacactgg taagagaaat acgccagtac cgaatgaaaa
      601 aactcaactg atgacgaagc ttgcggccgc actcgagcac caccaccacc accactgaga
      661 tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata
      721 actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg
      781 aactatatcc ggattggcga atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt
      841 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
      901 gc
//
