LOCUS       sequence-313363-        2679 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Overexpresses CLCN6.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2679)
  TITLE     These plasmids allow lentivirus-mediated overexpression of 
            understudied V5 tagged ion channels in mammalian cell lines.
REFERENCE   2  (bases 1 to 2679)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: IDG_CLCN6_OE_1
FEATURES             Location/Qualifiers
     source          1..2679
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(2609..2633)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2635..2676
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atggcggggt gcagggggtc tctgtgctgc tgctgcaggt ggtgctgctg ctgcggtgag
       61 cgtgagaccc gcacccccga ggagctgacc atccttggag aaacacagga ggaggaggat
      121 gagattcttc caaggaaaga ctatgagagt ttggattatg atcgctgtat caatgaccct
      181 tacctggaag ttttggagac catggataat aagaaaggtc gaagatatga ggcggtgaag
      241 tggatggtgg tgtttgccat tggagtctgc actggcctgg tgggtctctt tgtggacttt
      301 tttgtgcgac tcttcaccca actcaagttc ggagtggtac agacatcggt ggaggagtgc
      361 agccagaaag gctgcctcgc tctgtctctc cttgaactcc tgggttttaa cctcaccttt
      421 gtcttcctgg caagcctcct tgttctcatt gagccggtgg cagcaggttc cgggataccc
      481 gaggtcaaat gctatctgaa tggcgtaaag gtgccaggaa tcgtccgtct ccggaccctg
      541 ctctgcaagg tccttggagt gctgttcagt gtggctggag ggctcttcgt ggggaaggaa
      601 ggccccatga tccacagtgg ttcggtggtg ggagctggcc tccctcagtt tcagagcatc
      661 tccttacgga agatccagtt taacttcccc tatttccgaa gcgacagaga caagagagac
      721 tttgtatcag caggagcggc tgctggagtt gctgcagctt tcggggcgcc aatcgggggt
      781 accttgttca gtctagagga gggttcgtcc ttctggaacc aagggctcac gtggaaagtg
      841 ctcttttgtt ccatgtctgc caccttcacc ctcaacttct tccattctgg gattcagttt
      901 ggaagctggg gttccttcca gctccctgga ttgctgaact ttggcgagtt taagtgctct
      961 gactctgata aaaaatgtca tctctggaca gctatggatt tgggtttctt cgtcgtgatg
     1021 ggggtcattg ggggcctcct gggagccaca ttcaactgtc tgaacaagag gcttgcaaag
     1081 taccgtatgc gaaacgtgca cccgaaacct aagctcgtca gagtcttaga gagcctcctt
     1141 gtgtctctgg taaccaccgt ggtggtgttt gtggcctcga tggtgttagg agaatgccga
     1201 cagatgtcct cttcgagtca aatcggtaat gactcattcc agctccaggt cacagaagat
     1261 gtgaattcaa gtatcaagac atttttttgt cccaatgata cctacaatga catggccaca
     1321 ctcttcttca acccgcagga gtctgccatc ctccagctct tccaccagga tggtactttc
     1381 agccccgtca ctctggcctt gttcttcgtt ctctatttct tgcttgcatg ttggacttac
     1441 ggcatttctg ttccaagtgg cctttttgtg ccttctctgc tgtgtggagc tgcttttgga
     1501 cgtttagttg ccaatgtcct aaaaagctac attggattgg gccacatcta ttcggggacc
     1561 tttgccctga ttggtgcagc ggctttcttg ggcggggtgg tccgcatgac catcagcctc
     1621 acggtcatcc tgatcgagtc caccaatgag atcacctacg ggctccccat catggtcaca
     1681 ctgatggtgg ccaaatggac aggggacttt ttcaataagg gcatttatga tatccacgtg
     1741 ggcctgcgag gcgtgccgct tctggaatgg gagacagagg tggaaatgga caagctgaga
     1801 gccagcgaca tcatggagcc caacctgacc tacgtctacc cgcacacccg catccagtct
     1861 ctggtgagca tcctgcgcac cacggtccac catgccttcc cggtggtcac agagaaccgc
     1921 ggtaacgaga aggagttcat gaagggcaac cagctcatca gcaacaacat caagttcaag
     1981 aaatccagca tcctcacccg ggctggcgag cagcgcaaac ggagccagtc catgaagtcc
     2041 tacccatcca gcgagctacg gaacatgtgt gatgagcaca tcgcctctga ggagccagcc
     2101 gagaaggagg acctcctgca gcagatgctg gaaaggagat acactcccta ccccaaccta
     2161 taccctgacc agtccccaag tgaagactgg accatggagg agcggttccg ccctctgacc
     2221 ttccacggcc tgatccttcg gtcgcagctt gtcaccctgc ttgtccgagg agtttgttac
     2281 tctgaaagcc agtcgagcgc cagccagccg cgcctctcct atgccgagat ggccgaggac
     2341 tacccgcggt accccgacat ccacgacctg gacctgacgc tgctcaaccc gcgcatgatc
     2401 gtggatgtca ccccatacat gaacccttcg cctttcaccg tctcgcccaa cacccacgtc
     2461 tcccaagtct tcaacctgtt cagaacgatg ggcctgcgcc acctgcccgt ggtgaacgct
     2521 gtgggagaga tcgtggggat catcacacgg cacaacctca cctatgaatt tctgcaggcc
     2581 cggctgaggc agcactacca gaccatcttg ccaactttct tgtacaaagt ggttggtaag
     2641 cctatcccta accctctcct cggtctcgat tctacgtag
//
