LOCUS       sequence-313365-        1986 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Overexpresses SCNN1D.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1986)
  TITLE     These plasmids allow lentivirus-mediated overexpression of 
            understudied V5 tagged ion channels in mammalian cell lines.
REFERENCE   2  (bases 1 to 1986)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: IDG_SCNN1D_OE_1
FEATURES             Location/Qualifiers
     source          1..1986
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(1916..1940)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1942..1983
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atggctgagc accgaagcat ggacgggaga atggaagcag ccacacgggg gggctctcac
       61 ctccaggctg cagcccagac gccccccagg ccggggccac catcagcacc accaccacca
      121 cccaaggagg ggcaccagga ggggctggtg gagctgcccg cctcgttccg ggagctgctc
      181 accttcttct gcaccaatgc caccatccac ggcgccatcc gcctggtctg ctcccgcggg
      241 aaccgcctca agacgacgtc ctgggggctg ctgtccctgg gagccctggt cgcgctctgc
      301 tggcagctgg ggctcctctt tgagcgtcac tggcaccgcc cggtcctcat ggccgtctct
      361 gtgcactcgg agcgcaagct gctcccgctg gtcaccctgt gtgacgggaa cccacgtcgg
      421 ccgagtccgg tcctccgcca tctggagctg ctggacgagt ttgccaggga gaacattgac
      481 tccctgtaca acgtcaacct cagcaaaggc agagccgccc tctccgccac tgtcccccgc
      541 cacgagcccc ccttccacct ggaccgggag atccgtctgc agaggctgag ccactcgggc
      601 agccgggtca gagtggggtt cagactgtgc aacagcacgg gcggcgactg cttttaccga
      661 ggctacacgt caggcgtggc ggctgtccag gactggtacc acttccacta tgtggatatc
      721 ctggccctgc tgcccgcggc atgggaggac agccacggga gccaggacgg ccacttcgtc
      781 ctctcctgca gttacgatgg cctggactgc caggcccgac agttccggac cttccaccac
      841 cccacctacg gcagctgcta cacggtcgat ggcgtctgga cagctcagcg ccccggcatc
      901 acccacggag tcggcctggt cctcagggtt gagcagcagc ctcacctccc tctgctgtcc
      961 acgctggccg gcatcagggt catggttcac ggccgtaacc acacgccctt cctggggcac
     1021 cacagcttca gcgtccggcc agggacggag gccaccatca gcatccgaga ggacgaggtg
     1081 caccggctcg ggagccccta cggccactgc accgccggcg gggaaggcgt ggaggtggag
     1141 ctgctacaca acacctccta caccaggcag gcctgcctgg tgtcctgctt ccagcagctg
     1201 atggtggaga cctgctcctg tggctactac ctccaccctc tgccggcggg ggctgagtac
     1261 tgcagctctg cccggcaccc tgcctgggga cactgcttct accgcctcta ccaggacctg
     1321 gagacccacc ggctcccctg tacctcccgc tgccccaggc cctgcaggga gtctgcattc
     1381 aagctctcca ctgggacctc caggtggcct tccgccaagt cagctggatg gactctggcc
     1441 acgctaggtg aacaggggct gccgcatcag agccacagac agaggagcag cctggccaaa
     1501 atcaacatcg tctaccagga gctcaactac cgctcagtgg aggaggcgcc cgtgtactcg
     1561 gtgccgcagc tgctctcggc catgggcagc ctctgcagcc tgtggtttgg ggcctccgtc
     1621 ctctccctcc tggagctcct ggagctgctg ctcgatgctt ctgccctcac cctggtgcta
     1681 ggcggccgcc ggctccgcag ggcgtggttc tcctggccca gagccagccc tgcctcaggg
     1741 gcgtccagca tcaagccaga ggccagtcag atgcccccgc ctgcaggcgg cacgtcagat
     1801 gacccggagc ccagcgggcc tcatctccca cgggtgatgc ttccaggggt tctggcggga
     1861 gtctcagccg aagagagctg ggctgggccc cagccccttg agactctgga caccttgcca
     1921 actttcttgt acaaagtggt tggtaagcct atccctaacc ctctcctcgg tctcgattct
     1981 acgtag
//
